scholarly journals Development and molecular characterization of wheat-Aegilops peregrina Introgression Lines with resistance to leaf rust and stripe rust

2017 ◽  
Author(s):  
Deepika Narang ◽  
Satinder Kaur ◽  
Jyoti Saini ◽  
Parveen Chhuneja
Keyword(s):  
Leaf Rust ◽  
Molecular Characterization ◽  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Puccinia Triticina ◽  
Introgression Lines ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Plant Stage

AbstractA wild non-progenitor species from wheat tertiary gene pool Aegilops peregrina accession pau3519 (UUSS) was used for introgression of leaf rust and stripe rust resistance in bread wheat. It was crossed and backcrossed with hexaploid wheat line Chinese Spring PhI to develop two homozygous BC2F6 wheat-Ae. peregrina introgression lines (ILs) viz. IL pau16058 and IL pau16061 through induced homoeologous recombination. Homozygous lines were screened against six Puccinia triticina and two Puccinia striiformis f. sp. tritici pathotypes at the seedling stage and a mixture of prevalent pathotypes of both rust pathogens at the adult plant stage. IL pau16061 showed resistance to leaf rust only while IL pau16058 was resistant to both leaf and stripe rust pathotypes throughout plant life. Molecular characterization of these ILs aided in defining the introgressed regions. Identification of linked markers with advance genomic technologies will aid in marker assisted pyramiding of alien genes in cultivated wheat background.

scholarly journals Development and Molecular Characterization of Wheat-Aegilops peregrina Introgression Lines with Resistance to Leaf Rust and Stripe Rust

2017 ◽  
Vol 32 (1) ◽  
pp. 59-70 ◽  
Author(s):  
Deepika Narang ◽  
Satinder Kaur ◽  
Jyoti Saini ◽  
Parveen Chhuneja
Keyword(s):  
Leaf Rust ◽  
Molecular Characterization ◽  
Stripe Rust ◽  
Introgression Lines

scholarly journals A Genome-Wide Association Study of Resistance to Puccinia striiformis f. sp. hordei and P. graminis f. sp. tritici in Barley and Development of Resistant Germplasm

2020 ◽  
Vol 110 (5) ◽  
pp. 1082-1092 ◽  
Author(s):  
Javier Hernandez ◽  
Alicia del Blanco ◽  
Tanya Filichkin ◽  
Scott Fisk ◽  
Lynn Gallagher ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Stem Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Seedling Stage ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Adult Plant Stage ◽  
A Genome ◽  
Plant Stage

Stripe rust (incited by Puccinia striiformis f. sp. hordei) and stem rust (incited by P. graminis f. sp. tritici) are two of the most important diseases affecting barley. Building on prior work involving the introgression of the resistance genes rpg4/Rpg5 into diverse genetic backgrounds and the discovery of additional quantitative trait locus (QTLs) for stem rust resistance, we generated an array of germplasm in which we mapped resistance to stripe rust and stem rust. Stem rust races TTKSK and QCCJB were used for resistance mapping at the seedling and adult plant stages, respectively. Resistance to stripe rust, at the adult plant stage, was determined by QTLs on chromosomes 1H, 4H, and 5H that were previously reported in the literature. The rpg4/Rpg5 complex was validated as a source of resistance to stem rust at the seedling stage. Some parental germplasm, selected as potentially resistant to stem rust or susceptible but having other positive attributes, showed resistance at the seedling stage, which appears to be allelic to rpg4/Rpg5. The rpg4/Rpg5 complex, and this new allele, were not sufficient for adult plant resistance to stem rust in one environment. A QTL on 5H, distinct from Rpg5 and a previously reported resistance QTL, was required for resistance at the adult plant stage in all environments. This QTL is coincident with the QTL for stripe rust resistance. Germplasm with mapped genes/QTLs conferring resistance to stripe and stem rust was identified and is available as a resource to the research and breeding communities.

Inheritance and QTL analysis of durable resistance to stripe and leaf rusts in an Australian cultivar, Triticum aestivum 'Cook'

Genome ◽  
2005 ◽  
Vol 48 (1) ◽  
pp. 97-107 ◽  
Author(s):  
A Navabi ◽  
J P Tewari ◽  
R P Singh ◽  
B McCallum ◽  
A Laroche ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Stripe Rust ◽  
Qtl Analysis ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Durable Resistance ◽  
Puccinia Triticina ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Temperature Sensitive

An F4-derived F6 recombinant inbred line population (n = 148) of a cross between the durable stripe (yellow) rust (caused by Puccinia striiformis) and leaf (brown) rust (caused by Puccinia triticina) resistant cultivar, Triticum aestivum 'Cook', and susceptible genotype Avocet-YrA was phenotyped at several locations in Canada and Mexico under artificial epidemics of leaf or stripe rusts and genotyped using amplified fragment length polymorphism (AFLP) and microsatellite markers. Durable adult plant resistance to stripe and leaf rusts in 'Cook' is inherited quantitatively and was based on the additive interaction of linked and (or) pleiotropic slow-rusting genes Lr34 and Yr18 and the temperature-sensitive stripe rust resistance gene, YrCK, with additional genetic factors. Identified QTLs accounted for 18% to 31% of the phenotypic variation in leaf and stripe rust reactions, respectively. In accordance with the high phenotypic associations between leaf and stripe rust resistance, some of the identified QTLs appeared to be linked and (or) pleiotropic for both rusts across tests. Although a QTL was identified on chromosome 7D with significant effects on both rusts at some testing locations, it was not possible to refine the location of Lr34 or Yr18 because of the scarcity of markers in this region. The temperature-sensitive stripe rust resistance response, conditioned by the YrCK gene, significantly contributed to overall resistance to both rusts, indicating that this gene also had pleiotropic effects.Key words: wheat, rust diseases, Puccinia striiformis, Puccinia triticina, durable resistance, leaf-tip necrosis, QTL analysis.

Evaluation of Central Asian wheat germplasm for stripe rust resistance

2017 ◽  
Vol 16 (2) ◽  
pp. 178-184 ◽  
Author(s):  
Alma Kokhmetova ◽  
Ram C. Sharma ◽  
Shynbolat Rsaliyev ◽  
Kanat Galymbek ◽  
Kanagat Baymagambetova ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Breeding Lines ◽  
Central Asian ◽  
Plant Stage ◽  
Breeding Programmes ◽  
New Varieties

AbstractStripe rust, caused by Puccinia striiformis f.sp. tritici (Pst), is an important disease of winter wheat in Central Asia. Stripe rust races contain diverse virulence/avirulence patterns and change rapidly. Therefore the objectives of this research were to: (i) examine current pathotype variability of Pst races collected from Kazakhstan and Uzbekistan and (ii) evaluate stripe rust resistance in leading cultivars and advanced breeding lines targeted to those regions. Analyses of 152 Pst samples showed diverse virulence patterns with avirulence to Yr5, Yr10 and Yr15 being common. Most of identified races are among the rare. Analysis of a mixed Pst population showed 10 distinct pathotypes with frequencies ranged from 1.2 to 8.7%. The virulence patterns ranged from least ‘31–1.5’ and X-1.5 to highly virulent ‘86 + E16’. Seedling evaluation of 62 genotypes using the 10 pathotypes showed variations for resistance. Bunyodkor and Barhayot showed resistance to all pathotypes. Five Yr genes were postulated. Yr1 in KR12-5075, and Yr6 in KR11-03 and KR12-5003 were postulated. Yr5 combined with Yr10 and Yr15 genes were determined in Bunyodkor. The wheat genotypes also showed different levels of resistance in adult plant stage under field conditions. Twenty genotypes showed <20% severity in both Kazakhstan and Uzbekistan. The disease severity on several genotypes differed in this countries, suggesting different Pst populations in the two countries. Several resistant genotypes were identified, which should be further evaluated for release as new varieties or used in breeding programmes. Two resistant lines from this study were identified as new varieties in Georgia and Uzbekistan.

scholarly journals Inheritance of Leaf Rust and Stripe Rust Resistance in the Brazilian Wheat Cultivar ‘Toropi’

Plant Disease ◽  
2016 ◽  
Vol 100 (6) ◽  
pp. 1132-1137 ◽  
Author(s):  
Silvia B. Rosa ◽  
Brent McCallum ◽  
Anita Brûlé-Babel ◽  
Colin Hiebert ◽  
Stephen Shorter ◽  
...  
Keyword(s):  
New Zealand ◽  
Leaf Rust ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Doubled Haploid Population

Leaf rust (Puccinia triticina) and stripe rust (P. striiformis f. tritici) affect wheat production worldwide. Brazilian ‘Toropi’ wheat has demonstrated durable leaf rust resistance in South America since its release in 1965. It was previously found to have up to two adult plant leaf rust resistance genes. The leaf and stripe rust resistance of Toropi were studied by analyzing a doubled-haploid population made by crossing with susceptible ‘Thatcher’. Toropi expressed good resistance to leaf rust in Canada, Brazil, and New Zealand. Based on field and greenhouse testing, the leaf rust resistance of Toropi is conferred by two race-nonspecific complementary adult plant genes and a race-specific adult plant gene. The stripe rust resistance of Toropi analyzed in New Zealand and in Canada is based on up to two resistance genes. Toropi should provide an important contribution to rust resistance because it expressed good leaf rust and stripe rust resistance in different parts of the world.

scholarly journals Molecular mapping as a tool for pre-emptive breeding for resistance to the exotic barley pathogen, Puccinia striiformis f. sp. hordei

2003 ◽  
Vol 54 (12) ◽  
pp. 1351 ◽  
Author(s):  
M. Cakir ◽  
M. Spackman ◽  
C. R. Wellings ◽  
N. W. Galwey ◽  
D. B. Moody ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Molecular Mapping ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Sources Of Resistance ◽  
Plant Stage ◽  
The Usa ◽  
Alternative Sources ◽  
Barley Stripe Rust

Barley stripe rust (BSR), caused by Puccinia striiformis f. sp. hordei, has been a major disease problem to the barley industry worldwide that has not, to date, been detected in Australia. This paper describes the mapping of stripe rust resistance genes in Tallon/Kaputar (TK) and Arapiles/Franklin (AF) populations. The paper also reports on the usefulness of markers associated with alternative sources of resistance previously identified in the varieties Orca and Shyri in the USA. Stripe rust screening was conducted at the adult plant stage in Toluca, Mexico, for 2 years. Two major quantitative trait loci (QTLs) were found on chromosomes 2H and 5H in both populations. One region on chromosome 5H was highly significantly associated with resistance to stripe rust (R2 = 68% and 34% in TK and AF, respectively). The QTL on chromosome 2H accounted for 36% of the variation in TK and 10% of the variation in the AF population. These associations were consistent over both years. Further work will involve screening for additional markers in the target regions to identify polymorphism that can be used to select for multiple resistances in the absence of the pathogen.

Characterization of genetic loci conferring adult plant resistance to leaf rust and stripe rust in spring wheat

Genome ◽  
2006 ◽  
Vol 49 (8) ◽  
pp. 977-990 ◽  
Author(s):  
H M William ◽  
R P Singh ◽  
J Huerta-Espino ◽  
G Palacios ◽  
K Suenaga
Keyword(s):  
Leaf Rust ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Adult Plant Resistance ◽  
Puccinia Striiformis ◽  
Bulked Segregant Analysis ◽  
Puccinia Triticina ◽  
Adult Plant ◽  
Single Chromosome ◽  
Slow Rusting

Leaf (brown) and stripe (yellow) rusts, caused by Puccinia triticina and Puccinia striiformis, respectively, are fungal diseases of wheat (Triticum aestivum) that cause significant yield losses annually in many wheat-growing regions of the world. The objectives of our study were to characterize genetic loci associated with resistance to leaf and stripe rusts using molecular markers in a population derived from a cross between the rust-susceptible cultivar 'Avocet S' and the resistant cultivar 'Pavon76'. Using bulked segregant analysis and partial linkage mapping with AFLPs, SSRs and RFLPs, we identified 6 independent loci that contributed to slow rusting or adult plant resistance (APR) to the 2 rust diseases. Using marker information available from existing linkage maps, we have identified additional markers associated with resistance to these 2 diseases and established several linkage groups in the 'Avocet S' × 'Pavon76' population. The putative loci identified on chromosomes 1BL, 4BL, and 6AL influenced resistance to both stripe and leaf rust. The loci on chromosomes 3BS and 6BL had significant effects only on stripe rust, whereas another locus, characterized by AFLP markers, had minor effects on leaf rust only. Data derived from Interval mapping indicated that the loci identified explained 53% of the total phenotypic variation (R2) for stripe rust and 57% for leaf rust averaged across 3 sets of field data. A single chromosome recombinant line population segregating for chromosome 1B was used to map Lr46/Yr29 as a single Mendelian locus. Characterization of slow-rusting genes for leaf and stripe rust in improved wheat germplasm would enable wheat breeders to combine these additional loci with known slow-rusting loci to generate wheat cultivars with higher levels of slow-rusting resistance.Key words: Puccinia triticina, Puccinia striiformis, Triticum aestivum, bulked segregant analysis, single chromosome recombinant lines, linkage mapping, adult plant resistance.

scholarly journals QTL Mapping of Adult-Plant Resistance to Leaf and Stripe Rust in Wheat Cross SW 8588/Thatcher using the Wheat 55K SNP Array

Plant Disease ◽  
2019 ◽  
Vol 103 (12) ◽  
pp. 3041-3049 ◽  
Author(s):  
Peipei Zhang ◽  
Xing Li ◽  
Takele-Weldu Gebrewahid ◽  
Hexing Liu ◽  
Xianchun Xia ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Ssr Markers ◽  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Durable Resistance ◽  
Snp Array ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Sichuan Province ◽  
Adult Plant

Wheat leaf rust (caused by Puccinia triticina) and stripe rust (caused by Puccinia striiformis f. sp. tritici) cause large production losses in many regions of the world. The objective of this study was to identify quantitative trait loci (QTL) for resistance to leaf rust and stripe rust in a recombinant inbred line population derived from a cross between wheat cultivars SW 8588 and Thatcher. The population and parents were genotyped with the Wheat 55K SNP Array and SSR markers and phenotyped for leaf rust severity at Zhoukou in Henan Province and Baoding in Hebei Province. Stripe rust responses were also evaluated at Chengdu in Sichuan Province, and at Baoding. Seven and six QTL were detected for resistance to leaf rust and stripe rust, respectively. Four QTL on chromosomes 1BL, 2AS, 5AL, and 7BL conferred resistance to both rusts. The QTL on 1BL and 2AS were identified as Lr46/Yr29 and Lr37/Yr17, respectively. QLr.hebau-2DS from Thatcher, identified as Lr22b that was previously thought to be ineffective in China, contributed a large effect for leaf rust resistance. QLr.hebau-5AL/QYr.hebau-5AL, QLr.hebau-3BL, QLr.hebau-6DS, QYr.hebau-4BS, and QYr.hebau-6DS are likely to be new QTL, but require further validation. Kompetitive allele-specific PCR (KASP) markers for QLr.hebau-2DS and QLr.hebau-5AL/QYr.hebau-5AL were successfully developed and validated in a diverse wheat panel from Sichuan Province, indicating their usefulness under different genetic backgrounds. These QTL and their closely linked SNP and SSR markers will be useful for fine mapping, candidate gene discovery, and marker-assisted selection in breeding for durable resistance to both leaf and stripe rusts.

Inheritance of temperature-sensitive leaf rust resistance and adult plant stripe rust resistance in common wheat cultivar PBW343

Euphytica ◽  
2008 ◽  
Vol 166 (2) ◽  
pp. 277-282 ◽  
Author(s):  
Dibendu Datta ◽  
S. K. Nayar ◽  
M. Prashar ◽  
S. C. Bhardwaj
Keyword(s):  
Leaf Rust ◽  
Common Wheat ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Wheat Cultivar ◽  
Leaf Rust Resistance ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Temperature Sensitive ◽  
Common Wheat Cultivar
Sign in / Sign up

Export Citation Format

Share Document