scholarly journals Draft Genome Resources for the Phytopathogenic Fungi Monilinia fructicola, M. fructigena, M. polystroma, and M. laxa, the Causal Agents of Brown Rot

2018 ◽  
Vol 108 (10) ◽  
pp. 1141-1142 ◽  
Author(s):  
Yazmín Rivera ◽  
Kurt Zeller ◽  
Subodh Srivastava ◽  
Jeremy Sutherland ◽  
Marco Galvez ◽  
...  
Keyword(s):  
Draft Genome ◽  
Brown Rot ◽  
Phytopathogenic Fungi ◽  
Monilinia Fructicola ◽  
Pome Fruits ◽  
Rot Disease ◽  
Genome Assemblies

Fungi in the genus Monilinia cause brown rot disease of stone and pome fruits. Here, we report the draft genome assemblies of four important phytopathogenic species: M. fructicola, M. fructigena, M. polystroma, and M. laxa. The draft genome assemblies were 39 Mb (M. fructigena), 42 Mb (M. laxa), 43 Mb (M. fructicola), and 45 Mb (M. polystroma) with as few as 550 contigs (M. laxa). These are the first draft genome resources publicly available for M. laxa, M. fructigena, and M. polystroma.

Cloning and differential expression of a plum single repeat-MYB, PdMYB3, in compatible and incompatible interactions during fungal infection

2013 ◽  
Vol 93 (4) ◽  
pp. 599-605 ◽  
Author(s):  
Ashraf El-kereamy ◽  
Subramanian Jayasankar
Keyword(s):  
Fungal Infection ◽  
Differential Expression ◽  
Potential Role ◽  
Brown Rot ◽  
Myb Transcription Factor ◽  
Economic Damage ◽  
Monilinia Fructicola ◽  
Resistant Varieties ◽  
Rot Disease ◽  
Incompatible Interactions

El-kereamy, A. and Jayasankar, S. 2013. Cloning and differential expression of a plum single repeat-MYB, PdMYB3, in compatible and incompatible interactions during fungal infection. Can. J. Plant Sci. 93: 599–605. Enhancing resistance to pathogen attack through conventional breeding is a major challenge, especially in perennial species. Monilinia fructicola fungal infection causes brown rot disease, resulting in economic damage of stone fruits at flowering, pre- and post-harvest stages. The molecular mechanism of resistance to this disease is still not known. In the present study, we cloned and analyzed the expression of a novel MYB transcription factor from European plums (PdMYB3) induced in response to M. fructicola fungal infection. The identified PdMYB3 is a single repeat-MYB protein that contains a conserved SHAQKYF motif. Monilinia fructicola infection induces the expression of PdMYB3 in fruits of four cultivars within 24 h; however, it is differentially expressed in the susceptible and resistant varieties. By comparing four different cultivars we found that PdMYB3 is induced in much higher levels in the susceptible cultivars than the resistant ones. In addition the PdMYB3 expression is higher in the early stages of fruit development prior to pit hardening, suggesting a potential role for PdMYB3 during this stage. Promoter analysis revealed the presence of some hormone cis-elements suggesting a possible role for PdMYB3 gene in transmitting a signal from the hormonal pathways to downstream components during host-pathogen interactions.

scholarly journals Mating System in the Brown Rot Pathogens Monilinia fructicola, M. laxa, and M. fructigena

2018 ◽  
Vol 108 (11) ◽  
pp. 1315-1325 ◽  
Author(s):  
Domenico Abate ◽  
Rita M. De Miccolis Angelini ◽  
Caterina Rotolo ◽  
Stefania Pollastro ◽  
Francesco Faretra
Keyword(s):  
Mating System ◽  
Population Biology ◽  
Constitutive Expression ◽  
Brown Rot ◽  
Antisense Transcription ◽  
Inter Simple Sequence Repeat ◽  
Monilinia Fructicola ◽  
Specific Pcr ◽  
Rot Disease ◽  
Flanking Regions

Monilinia fructicola, M. laxa, and M. fructigena are the most important pathogens responsible for brown rot disease of stone and pome fruits. Information on their mating system and sexual behavior is scant. A mating-type-specific PCR-based assay was developed and applied to 155 Monilinia isolates from 10 countries and 10 different host plants. We showed that single isolates carry only one of two opposite idiomorphs at the MAT1 locus consistent with a heterothallic mating system for all three species. MAT1-1 and MAT1-2 mating types were detected in similar proportions in samples of isolates of each species and hence there do not appear to be genetic obstacles to the occurrence of sexual reproduction in their populations. Inter simple sequence repeat markers suggested that asexual reproduction is prevalent, but that sexual recombination occurs in M. fructicola populations in Italy. The genetic architectures of the MAT1 loci of the three pathogens were analyzed. MAT1-1 and MAT1-2 idiomorphs are flanked upstream and downstream by the APN2 and SLA2 genes and resemble those of Botrytis cinerea and other heterothallic fungi in the family Sclerotiniaceae. Each idiomorph contains a specific couple of genes, MAT1-1-1 (with alpha-box domain) and MAT1-1-5 in MAT1-1, and MAT1-2-1 (with HMG-box domain) and MAT1-2-10 in MAT1-2. Small gene fragments (dMAT1-1-1 and dMAT1-2-1) from the opposite idiomorph were detected close to their flanking regions. Constitutive expression of the four MAT1 genes during vegetative growth was ascertained by transcriptomic analysis (RNA-Seq). Antisense transcription of the MAT1-1-1 and MAT1-2-1 genes and intergenic transcribed regions of the MAT1 locus were detected. These results represent new insights into the mating systems of these three economically-important pathogens which could contribute to improve the knowledge on their population biology.

scholarly journals New High-Quality Draft Genome of the Brown Rot Fungal Pathogen Monilinia fructicola

2019 ◽  
Vol 11 (10) ◽  
pp. 2850-2855 ◽  
Author(s):  
Rita Milvia De Miccolis Angelini ◽  
Gianfranco Romanazzi ◽  
Stefania Pollastro ◽  
Caterina Rotolo ◽  
Francesco Faretra ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Fungal Pathogen ◽  
Reference Genome ◽  
Draft Genome ◽  
Brown Rot ◽  
Monilinia Fructicola ◽  
Pome Fruit ◽  
High Quality ◽  
Draft Genome Assembly ◽  
Sequencing Technologies

Abstract Brown rot is a worldwide fungal disease of stone and pome fruit that is caused by several Monilinia species. Among these, Monilinia fructicola can cause severe preharvest and postharvest losses, especially for stone fruit. Here, we present a high-quality draft genome assembly of M. fructicola Mfrc123 strain obtained using both Illumina and PacBio sequencing technologies. The genome assembly comprised 20 scaffolds, including 29 telomere sequences at both ends of 10 scaffolds, and at a single end of 9 scaffolds. The total length was 44.05 Mb, with a scaffold N50 of 2,592 kb. Annotation of the M. fructicola assembly identified a total of 12,118 genes and 13,749 proteins that were functionally annotated. This newly generated reference genome is expected to significantly contribute to comparative analysis of genome biology and evolution within Monilinia species.

scholarly journals Characterization of Monilinia fructicola Strains Resistant to Both Propiconazole and Boscalid

Plant Disease ◽  
2013 ◽  
Vol 97 (5) ◽  
pp. 645-651 ◽  
Author(s):  
F. Chen ◽  
X. Liu ◽  
S. Chen ◽  
E. Schnabel ◽  
G. Schnabel
Keyword(s):  
Succinate Dehydrogenase ◽  
Disease Incidence ◽  
Mobile Element ◽  
Brown Rot ◽  
Monilinia Fructicola ◽  
Quinone Outside Inhibitor ◽  
Dehydrogenase Enzyme ◽  
Rot Disease ◽  
Succinate Dehydrogenase Inhibitor ◽  
Spartanburg County

In 2011 and 2012, significant brown rot disease caused by Monilinia fructicola was observed in a peach orchard in Spartanburg County, SC, despite preharvest fungicide applications of demethylation inhibitor (DMI), quinone outside inhibitor (QoI), and succinate dehydrogenase inhibitor (SDHI) fungicides. All 22 isolates obtained in 2011 from this orchard were sensitive to the QoI fungicide, azoxystrobin, and the methyl benzimidazole carbamate (MBC) fungicide, thiophanate-methyl. Five were resistant to the DMI fungicide, propiconazole, and were selected, together with five propiconazole-sensitive isolates, for further investigations. One of the 10 isolates was resistant to propiconazole but sensitive to the SDHI fungicide, boscalid (EC50 = 0.42 μg/ml), 3 were resistant to propiconazole with intermediate sensitivity to boscalid (EC50 0.72 to 2.1 μg/ml); 2 were sensitive to propiconazole with intermediate sensitivity to boscalid; 3 were sensitive to propiconazole but resistant to boscalid (EC50 ≥ 2.1 μg/ml); and 1 (isolate MD22) was resistant to both propiconazole and boscalid. Disease incidence on detached fruit treated with formulated propiconazole or boscalid was significantly higher for MD22 compared to a sensitive control isolate. Continued monitoring of fungicide resistance in the same orchard in 2012 revealed an increase of isolates resistant to propiconazole from 22.7% in 2011 to 34.7%, and an increase of isolates resistant to both propiconazole and boscalid from 4.5% in 2011 to 18.4%. Propiconazole resistance was always associated with the presence of the ‘Mona’ mobile element located upstream of the sterol 14α-demethylase (MfCYP51) gene. To investigate whether mutations in the subunits of the succinate dehydrogenase enzyme were involved in boscalid resistance, significant portions of the M. fructicola SdhA, SdhB, SdhC, and SdhD genes were cloned and analyzed for 2 sensitive, 2 boscalid-resistant, and 6 dual-resistant isolates. Although sequence variation was found among the isolates, no single change correlated with resistance. Interestingly, analysis of isolates collected from orchards in 2001 and 2002, prior to the registration of boscalid, revealed a range of sensitivities to boscalid (EC50 0.03 to 3.46 μg/ml) including boscalid-resistant isolates. The presence of boscalid-resistant isolates in the baseline population was unexpected and requires further investigation.

Antifungal mechanisms of lavender essential oil in the inhibition of rot disease caused by Monilinia fructicola in postharvest flat peaches

2021 ◽  
Author(s):  
Xiaolin Xiong ◽  
Lingling Zhang ◽  
Xingyan Li ◽  
Qingzhi Zeng ◽  
Rongrong Deng ◽  
...  
Keyword(s):  
Essential Oil ◽  
Membrane Damage ◽  
Phytopathogenic Fungi ◽  
Monilinia Fructicola ◽  
Inhibitory Effects ◽  
Rot Disease ◽  
Main Components ◽  
Fungistatic Effect

As a natural antimicrobial agent, lavender essential oil (LEO) is generally recognized to be safe and effective in the inhibition of phytopathogenic fungi. Direct contact and fumigation (in vivo and in vitro) were used to study the fungistatic effect of LEO on Monilinia fructicola. Additionally, the effect on the ultrastructure of cells and degree of destruction of the cell membrane of M. fructicola were revealed. In addition, the effects of LEO on the expression levels of particular apoptosis-related genes in M. fructicola cells were detected and GC-MS was used to analyse the main components of LEO. LEO had a good inhibitory efficacy against M. fructicola in flat peaches, with almost complete growth inhibition with 800 μL / L. These effects were associated with leakage of cytoplasm contents, hyphal distortion and spore disruption. Moreover, the expression of apoptosis RTG1 and RLM1 genes increased on LEO treatment. These results demonstrate that LEO can inhibit M. fructicola by inducing cytoplasmic membrane damage and cell apoptosis of fungi and that the major ingredients of LEO are monoterpenes and sesquiterpenes which are presumed to contribute to the inhibitory effects.

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