scholarly journals Variation in UV-mediated damage recovery among Pseudoidium neolycopersici isolates: possible mechanisms.

2021 ◽  
Author(s):  
Ranjana Pathak ◽  
Aruppillai Suthaparan
Keyword(s):  
Blue Light ◽  
Domain Architecture ◽  
Regulatory Elements ◽  
Core Domain ◽  
Conidia Germination ◽  
Biological Responses ◽  
Pseudoidium Neolycopersici ◽  
Uv C

The cryptochrome/photolyase family (CPF) consists of a diversified class of flavoproteins that are evolutionarily related. Although their domain architecture is highly conserved, they perform entirely different physiological functions. Previous studies have confirmed the presence of a functional photolyase in Pseudoidium neolycopersici, which repairs UV-C-induced DNA damage by using near UV-A/blue light as an energy source. Similar doses of UV-C treatment followed by incubation with dark or blue light was tested on conidia germination of five isolates of P. neolycopersici collected from different regions of Norway and the Netherlands. The results showed variations in the effect of UV on germination and germination recovery under blue light incubation after UV treatment. Evolutionary studies confirmed that P. neolycopersici photolyase is highly conserved among different isolates of P. neolycopersici and among different species. All CPF members have a core domain consisting of an identical cofactor Flavin adenine dinucleotide (FAD), and an additional photoantenna chromophore. An amino acid sequence analysis demonstrated that CPF members have highly conserved C-terminals compared with their N-terminals, as FAD binds in the C-terminal region. When compared with other CPF members, varying lengths of N- and C-terminals were noticed in P. neolycopersici photolyase and putative cryptochrome, respectively. Further research using comparative genomics targeting pyrimidine base composition, the role of regulatory elements including promotor architecture, and the characterization of optical properties of native photolyase among isolates may help to explain the differences in the biological responses of conidia germination of P. neolycopersici treated with UV.

Deciphering the specific role of Gαi/o isoforms: functional selective oxytocin ligands and somatostatin SST5 receptor mutants

2013 ◽  
Vol 41 (1) ◽  
pp. 166-171 ◽  
Author(s):  
Marta Busnelli ◽  
Erika Peverelli ◽  
Giovanna Mantovani ◽  
Anna Spada ◽  
Bice Chini
Keyword(s):  
Somatostatin Receptor ◽  
Receptor Activation ◽  
G Protein Coupled Receptors ◽  
Receptor Coupling ◽  
Biological Responses ◽  
Selective Ligands ◽  
Specific Effector ◽  
G Protein Coupled

Receptor coupling to different G-proteins and β-arrestins has been described for a number of GPCRs (G-protein-coupled receptors), suggesting a multi-state model of receptor activation in which each receptor can assume a number of different active conformations, each capable of promoting the coupling to a specific effector. Consistently, functional-selective ligands and biased agonists have been described to be able to induce and/or stabilize only a subset of specific active conformations. Furthermore, GPCR mutants deficient in selective coupling have been reported. Functional selective ligands and receptor mutants thus constitute unique tools to dissect the specific roles of different effectors, in particular among the Gi/o family. In the present mini-review, we focus on (i) the identification of functional selective OXT (oxytocin)-derived peptides capable of activating single Gi/o isoforms, namely Gi1 or Gi3; and (ii) the characterization of an SS (somatostatin) receptor SST5 mutant selectively impaired in its GoA coupling. These analogues and receptor mutants represent unique tools for examining the contribution of Gi/o isoforms in complex biological responses and open the way for the development of drugs with peculiar selectivity profiles.

scholarly journals TCTP from Loxosceles Intermedia (Brown Spider) Venom Contributes to the Allergic and Inflammatory Response of Cutaneous Loxoscelism

Cells ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 1489 ◽  
Author(s):  
Marianna Boia-Ferreira ◽  
Kamila G. Moreno ◽  
Alana B. C. Basílio ◽  
Lucas P. da Silva ◽  
Larissa Vuitika ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Dependent Manner ◽  
Dermonecrotic Toxin ◽  
Biological Responses ◽  
Brown Spider ◽  
Releasing Factors

LiTCTP is a toxin from the Translationally Controlled Tumor Protein (TCTP) family identified in Loxosceles brown spider venoms. These proteins are known as histamine-releasing factors (HRF). TCTPs participate in allergic and anaphylactic reactions, which suggest their potential role as therapeutic targets. The histaminergic effect of TCTP is related to its pro-inflammatory functions. An initial characterization of LiTCTP in animal models showed that this toxin can increase the microvascular permeability of skin vessels and induce paw edema in a dose-dependent manner. We evaluated the role of LiTCTP in vitro and in vivo in the inflammatory and allergic aspects that undergo the biological responses observed in Loxoscelism, the clinical condition after an accident with Loxosceles spiders. Our results showed LiTCTP recombinant toxin (LiRecTCTP) as an essential synergistic factor for the dermonecrotic toxin actions (LiRecDT1, known as the main toxin in the pathophysiology of Loxoscelism), revealing its contribution to the exacerbated inflammatory response clinically observed in envenomated patients.

Digital x-ray mapping of nanometer-size supported bimetallic catalysts

1988 ◽  
Vol 46 ◽  
pp. 530-531
Author(s):  
L. T. Germinario
Keyword(s):  
Background Radiation ◽  
Metal Cluster ◽  
Single Element ◽  
Beam Diameter ◽  
X Rays ◽  
X Ray ◽  
Major Interest ◽  
Induced Changes

Understanding the role of metal cluster composition in determining catalytic selectivity and activity is of major interest in heterogeneous catalysis. The electron microscope is well established as a powerful tool for ultrastructural and compositional characterization of support and catalyst. Because the spatial resolution of x-ray microanalysis is defined by the smallest beam diameter into which the required number of electrons can be focused, the dedicated STEM with FEG is the instrument of choice. The main sources of errors in energy dispersive x-ray analysis (EDS) are: (1) beam-induced changes in specimen composition, (2) specimen drift, (3) instrumental factors which produce background radiation, and (4) basic statistical limitations which result in the detection of a finite number of x-ray photons. Digital beam techniques have been described for supported single-element metal clusters with spatial resolutions of about 10 nm. However, the detection of spurious characteristic x-rays away from catalyst particles produced images requiring several image processing steps.

Consideraciones en torno a la reducción solipsista

1970 ◽  
Vol 11 ◽  
Author(s):  
Natalia Carolina Petrillo

ResumenEn el presente trabajo se intentará mostrar que la fenomenología no conduce a una postura solipsista. Para ello, se caracterizará en qué consiste el solipsismo. Luego, se intentará refutar a lo que se ha de llamar “solipsismo metafísico” y “solipsismo gnoseológico”, con el objetivo principal de poner de manifiesto el fundamento de motivación para la salida de la ficción solipsista.Palabras claves:Phenomenology – solipsim – empatía - HusserlAbstractWith the aim of showing that phenomenology does not lead in solipsism, I will first attempt a characterization of it. Then, I will attempt a refutation of the so-called “metaphysical” and “epistemological” solipsisms. Finally, the nature and role of Husserl´s solipsistic fiction is examined, and the grounds that motivate the overcoming of this standpoint are disclosed.key wordsFenomenología – solipsismo - empathy – Husserl

ART STUDIOS – A WAY TO DEVELOPE CHILDREN’S IMAGINATION

2020 ◽  
Vol 11 (1) ◽  
pp. 144-148
Author(s):  
Liuba Zlatkova ◽  
Keyword(s):  
Fairy Tale ◽  
Psychological Effects ◽  
Complete Product ◽  
The Arts ◽  
The Creation ◽  
Role Of Parents

The report describes the steps for creating a musical tale by children in the art studios of „Art Workshop“, Shumen. These studios are led by students volunteers related to the arts from pedagogical department of Shumen University, and are realized in time for optional activities in the school where the child studies. The stages of creating a complete product with the help of different arts are traced – from the birth of the idea; the creation of a fairy tale plot by the children; the characterization of the fairy-tale characters; dressing them in movement, song and speech; creating sets and costumes and creating a finished product to present on stage. The role of parents as a link and a necessary helper for children and leaders is also considered, as well as the positive psychological effects that this cooperation creates.

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