scholarly journals Assisted reproductive technologies in the common marmoset: an integral species for developing nonhuman primate models of human diseases†

2017 ◽  
Vol 96 (2) ◽  
pp. 277-287 ◽  
Author(s):  
Jenna Kropp ◽  
Andrea Di Marzo ◽  
Thaddeus Golos
Keyword(s):  
Nonhuman Primate ◽  
Assisted Reproductive Technologies ◽  
Common Marmoset ◽  
Reproductive Technologies ◽  
Human Diseases ◽  
The Common

scholarly journals Testing Efficacy of Administration of the Antiaging Drug Rapamycin in a Nonhuman Primate, the Common Marmoset

2014 ◽  
Vol 70 (5) ◽  
pp. 577-588 ◽  
Author(s):  
Suzette Tardif ◽  
Corinna Ross ◽  
Phillip Bergman ◽  
Elizabeth Fernandez ◽  
Marty Javors ◽  
...  
Keyword(s):  
Nonhuman Primate ◽  
Common Marmoset ◽  
The Common

Establishment of graded spinal cord injury model in a nonhuman primate: The common marmoset

2005 ◽  
Vol 80 (2) ◽  
pp. 172-181 ◽  
Author(s):  
A. Iwanami ◽  
J. Yamane ◽  
H. Katoh ◽  
M. Nakamura ◽  
S. Momoshima ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Nonhuman Primate ◽  
Common Marmoset ◽  
Spinal Cord Injury Model ◽  
Injury Model ◽  
Cord Injury ◽  
The Common

The Common Marmoset as a Target Preclinical Primate Model for Cytokine and Gene Therapy Studies

Blood ◽  
1999 ◽  
Vol 93 (9) ◽  
pp. 2839-2848 ◽  
Author(s):  
Hitoshi Hibino ◽  
Kenzaburo Tani ◽  
Kenji Ikebuchi ◽  
Ming-Shiuan Wu ◽  
Hajime Sugiyama ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Gene Transfer ◽  
Progenitor Cells ◽  
Nonhuman Primate ◽  
Peripheral Blood ◽  
Common Marmoset ◽  
Transduction Efficiency ◽  
Hematopoietic Stem ◽  
The Common

Nonhuman primate models are useful to evaluate the safety and efficacy of new therapeutic modalities, including gene therapy, before the inititation of clinical trials in humans. With the aim of establishing safe and effective approaches to therapeutic gene transfer, we have been focusing on a small New World monkey, the common marmoset, as a target preclinical model. This animal is relatively inexpensive and easy to breed in limited space. First, we characterized marmoset blood and bone marrow progenitor cells (BMPCs) and showed that human cytokines were effective to maintain and stimulate in culture. We then examined their susceptibility to transduction by retroviral vectors. In a mixed culture system containing both marmoset stromal cells and retroviral producer cells, the transduction efficiency into BMPCs and peripheral blood progenitor cells (PBPCs) was 12% to 24%. A series of marmosets then underwent transplantation with autologous PBPCs transduced with a retroviral vector carrying the multidrug resistance 1 gene (MDR1) and were followed for the persistence of these cells in vivo. Proviral DNA was detectable by polymerase chain reaction (PCR) in peripheral blood granulocytes and lymphocytes in the recipients of gene transduced progenitors up to 400 days posttransplantation. To examine the function of the MDR1 gene in vivo, recipient maromsets were challenged with docetaxel, an MDR effluxed drug, yet the overall level of gene transfer attained in vivo (<1% in peripheral blood granulocytes) was not sufficient to prevent the neutropenia induced by docetaxel treatment. Using this model, we safely and easily performed a series of in vivo studies in our small animal center. Our results show that this small nonhuman primate, the common marmoset, is a useful model for the evaluation of gene transfer methods targeting hematopoietic stem cells.

Advanced follicle development in xenografted prepubertal ovarian tissue: the common marmoset as a nonhuman primate model for ovarian tissue transplantation

2011 ◽  
Vol 95 (4) ◽  
pp. 1428-1434 ◽  
Author(s):  
Viktoria von Schönfeldt ◽  
Ramesh Chandolia ◽  
Ludwig Kiesel ◽  
Eberhard Nieschlag ◽  
Stefan Schlatt ◽  
...  
Keyword(s):  
Nonhuman Primate ◽  
Ovarian Tissue ◽  
Common Marmoset ◽  
Tissue Transplantation ◽  
Follicle Development ◽  
Nonhuman Primate Model ◽  
Primate Model ◽  
Ovarian Tissue Transplantation ◽  
The Common

scholarly journals Characterization of Obese Phenotypes in a Small Nonhuman Primate, the Common Marmoset (Callithrix jacchus )

Obesity ◽  
2009 ◽  
Vol 17 (8) ◽  
pp. 1499-1505 ◽  
Author(s):  
Suzette D. Tardif ◽  
Michael L. Power ◽  
Corinna N. Ross ◽  
Julienne N. Rutherford ◽  
Donna G. Layne-Colon ◽  
...  
Keyword(s):  
Nonhuman Primate ◽  
Common Marmoset ◽  
Callithrix Jacchus ◽  
The Common

scholarly journals Efficient marmoset genome engineering by autologous embryo transfer and CRISPR/Cas9 technology

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yukiko Abe ◽  
Harumi Nakao ◽  
Motoki Goto ◽  
Moe Tamano ◽  
Michinori Koebis ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Genome Engineering ◽  
Assisted Reproductive Technologies ◽  
Small Body ◽  
Common Marmoset ◽  
Reproductive Technologies ◽  
Reproductive Capacity ◽  
Primate Species ◽  
Novel Method

AbstractGenetic engineering of non-human primates, which are most closely related to humans, has been expected to generate ideal animal models for human genetic diseases. The common marmoset (Callithrix jacchus) is a non-human primate species adequate for the production of genetically modified animals because of their small body size and high reproductive capacity. Autologous embryo transfer (AET) is routinely utilized in assisted reproductive technologies for humans but not for experimental animals. This study has developed a novel method for efficiently producing mutant marmosets using AET and CRISPR/Cas9 systems. The embryos were recovered from oviducts of naturally mated females, injected with Cas9/guide RNA, and transferred into the oviducts of the donors. This AET method can reduce the time for in vitro culture of embryos to less than 30 min. This method uses an embryo donor as the recipient, thus reducing the number of animals and allowing for “Reduction” in the 3R principles of humane experimental technique. Furthermore, this method can utilize nulliparous females as well as parous females. We applied our novel method and generated the 6 marmosets carrying mutations in the fragile X mental retardation 1 (FMR1) gene using only 18 females including 14 nulliparous females.

scholarly journals Exploring the Innate Immunological Response of an Alternative Nonhuman Primate Model of Infectious Disease; the Common Marmoset

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
M. Nelson ◽  
M. Loveday
Keyword(s):  
Infectious Disease ◽  
Immune Response ◽  
Nonhuman Primate ◽  
Immune Cell ◽  
Common Marmoset ◽  
Cell Types ◽  
Nonhuman Primate Model ◽  
Primate Model ◽  
The Common ◽  
Activation Status

The common marmoset (Callithrix jacchus) is increasingly being utilised as a nonhuman primate model for human disease, ranging from autoimmune to infectious disease. In order to fully exploit these models, meaningful comparison to the human host response is necessary. Commercially available reagents, primarily targeted to human cells, were utilised to assess the phenotype and activation status of key immune cell types and cytokines in naive and infected animals. Single cell suspensions of blood, spleen, and lung were examined. Generally, the phenotype of cells was comparable between humans and marmosets, with approximately 63% of all lymphocytes in the blood of marmosets being T cells, 25% B-cells, and 12% NK cells. The percentage of neutrophils in marmoset blood were more similar to human values than mouse values. Comparison of the activation status of cells following experimental systemic or inhalational infection exhibited different trends in different tissues, most obvious in cell types active in the innate immune response. This work significantly enhances the ability to understand the immune response in these animals and fortifies their use as models of infectious disease.

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