scholarly journals In Vitro Production of Haploid Germ Cells from Fresh or Frozen-Thawed Testicular Cells of Neonatal Bulls1

2001 ◽  
Vol 65 (3) ◽  
pp. 873-878 ◽  
Author(s):  
Dong Ryul Lee ◽  
Michael T. Kaproth ◽  
John E. Parks
Keyword(s):  
Germ Cells ◽  
In Vitro Production ◽  
Testicular Cells ◽  
Vitro Production

scholarly journals Utilization of Oocytes Collected from Preserved Ovarian for In Vitro Production of Cat Embryos

2018 ◽  
Vol 10 (1) ◽  
pp. 160-168
Author(s):  
Kartini Eriani ◽  
Arief Boediono ◽  
Sony Heru Sumarsono ◽  
Al Azhar
Keyword(s):  
Germ Cells ◽  
Ovarian Tissue ◽  
Fertilization Rate ◽  
Culture Technique ◽  
In Vitro Production ◽  
Vitro Fertilization ◽  
Vitro Production ◽  
Preservation Technique ◽  
Medium Culture

Preservation of ovarian tissue from severely injured or dead valuable animals has the potential to preserve female germ cells of animals. The ability to mature and fertilize of oocytes from preserved ovary of endangered species will allow us to sustain genetic and global biodiversities. The aims of this study were to investigate the viability of oocytes collected from the preserved ovary and its potential utilization for the production of cat embryos followed by in vitro maturation and fertilization. Ovary was preserved immediately in phosphate buffer saline (PBS) at 4 °C for 24 or 48 hours. The quality and viability of oocytes after the maturation process were identified microscopically using aceto-orcein staining. Biological function of the oocytes was evaluated by using in vitro culture technique for the maturation and fertilization rate in CR1aa medium culture. The results showed that the percentage of oocytes collected from preserved ovary for 24 and 48 hours that remained at the stage of metaphase-II were 29.4% and 21.9% respectively. Fertilization rates produced in the IVF using oocytes collected from ovary preserved for 24 or 48 hours were significantly lower (30%) than that of unpreserved control (36.7%). In conclusion, female germ cells of cat ovary preserved at 4 °C in PBS for 2 days were still viable for in vitro fertilization and thus can be utilized for in vitro production of cat embryos. Information obtained can be used as a basis of knowledge of using a combination of physiological reagent and cold-based preservation technique in modern reproductive technology for animals.

scholarly journals PROSPECTS FOR THE USING OF SILICON-CONTAINING COMPOUNDS IN THE TECHNOLOGY OF IN VITRO MATURATION OF ANIMAL OOCYTES

2021 ◽  
Author(s):  
T.I. Kuzmina ◽  
D.N. Tatarskaya ◽  
V. YU Kravtsov
Keyword(s):  
Germ Cells ◽  
In Vitro Maturation ◽  
Bos Taurus ◽  
Culture Media ◽  
In Vitro Production ◽  
Positive Effects ◽  
Highly Dispersed Silica ◽  
Sus Scrofa Domesticus ◽  
Vitro Production

In vitro production of animal embryos is an important tool for solving male and female infertility problems in animals. Modeling of extracorporeal maturation systems of oocytes using siliconcontaining compounds in the composition of culture media revealed the peculiarities of the realization of their effects on somatic and germ cells of ovarian follicles, depending on the structure. Highly dispersed silica nanoparticles (nHDS) positive effects on the fertility of female gametes in Bos Taurus and Sus Scrofa Domesticus. The gel substrate of silica (silicon dimethylglycerolate - DMGC) does not cause an increase in the level of embryos cleavage, while it does not have cytoand genotoxicity when tested on somatic and germ cells of antral follicles.Key words: siliconcontaining compounds, in vitro maturation, animal oocytes.

In vitro production of haploid sperm cells from male germ cells of foetal cattle

2010 ◽  
Vol 118 (2-4) ◽  
pp. 103-109 ◽  
Author(s):  
Wu-Zi Dong ◽  
Jin-Lian Hua ◽  
Wen-Zheng Shen ◽  
Zhong-Ying Dou
Keyword(s):  
Germ Cells ◽  
Sperm Cells ◽  
In Vitro Production ◽  
Male Germ Cells ◽  
Vitro Production

In vitro production of functional haploid sperm cells from male germ cells of Saanen dairy goat

2017 ◽  
Vol 90 ◽  
pp. 120-128 ◽  
Author(s):  
Shoulong Deng ◽  
Xiuxia Wang ◽  
Zhipeng Wang ◽  
Suren Chen ◽  
Yuqian Wang ◽  
...  
Keyword(s):  
Germ Cells ◽  
Dairy Goat ◽  
Sperm Cells ◽  
In Vitro Production ◽  
Male Germ Cells ◽  
Vitro Production

THE IN VITRO PRODUCTION OF ANDROGENS BY FOLLICULAR TISSUE OF RABBITS

1964 ◽  
Vol 47 (2) ◽  
pp. 306-313 ◽  
Author(s):  
Denis Gospodarowicz
Keyword(s):  
In Vitro Production ◽  
Vitro Production

ABSTRACT Incubation in vitro of rabbit follicles in separate experiments with dehydroepiandrosterone-14C (DHEA-14C), progesterone-14C and pregnenolone-3H in the presence of FSH gave the following results: 39 % of the radioactivity of DHEA-14C is converted to androstenedione and testosterone, while only 3 % of the radioactivity of either progesterone-14C or pregnenolone-3H is found in the androgen fraction. From the ratio of testosterone to androstenedione formed from the three precursors, the results are interpreted to mean that DHEA and pregnenolone, and not progesterone, are precursors of androgens in the follicle.

Oxygenation of 18-hydroxycorticosterone as the final reaction for aldosterone biosynthesis

1984 ◽  
Vol 107 (3) ◽  
pp. 395-400 ◽  
Author(s):  
Itaru Kojima ◽  
Etsuro Ogata ◽  
Hiroshi Inano ◽  
Bun-ichi Tamaoki
Keyword(s):  
Carbon Monoxide ◽  
Hydroxysteroid Dehydrogenase ◽  
Dependent Manner ◽  
In Vitro Production ◽  
Mitochondrial Preparation ◽  
Final Reaction ◽  
Vitro Production ◽  
Dose Dependent ◽  
Cytochrome P 450

Abstract. Incubation of 18-hydroxycorticosterone with the sonicated mitochondrial preparation of bovine adrenal glomerulosa tissue leads to the production of aldosterone, as measured by radioimmunoassay. The in vitro production of aldosterone from 18-hydroxycorticosterone requires both molecular oxygen and NADPH, and is inhibited by carbon monoxide. Cytochrome P-450 inhibitors such as metyrapone, SU 8000. SU 10603, SKF 525A, amphenone B and spironolactone decrease the biosynthesis of aldosterone from 18-hydroxycorticosterone. These results support the conclusion that the final reaction in aldosterone synthesis from 18-hydroxycorticosterone is catalyzed by an oxygenase, but not by 18-hydroxysteroid dehydrogenase. By the same preparation, the production of [3H]aldosterone but not [3H]18-hydroxycorticosterone from [1,2-3H ]corticosterone is decreased in a dose-dependent manner by addition of non-radioactive 18-hydroxycorticosterone.

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