scholarly journals Cellular Compensation for Chronic Decreases in Cyclic AMP Concentration: Gαs‐Deficient Dendritic Cells as a Model and Implications for Allergic Disorder Therapeutics

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Amy M. Chinn ◽  
Jihyung Lee ◽  
Eyal Raz ◽  
Paul A. Insel
Keyword(s):  
Dendritic Cells ◽  
Cyclic Amp ◽  
Allergic Disorder

scholarly journals Cyclic AMP Modulates the Functional Plasticity of Immature Dendritic Cells by Inhibiting Src-like Kinases through Protein Kinase A-mediated Signaling

2004 ◽  
Vol 279 (31) ◽  
pp. 32507-32514 ◽  
Author(s):  
Mario Galgani ◽  
Veronica De Rosa ◽  
Salvatore De Simone ◽  
Antonio Leonardi ◽  
Ugo D'Oro ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Protein Kinase A ◽  
Functional Plasticity ◽  
Immature Dendritic Cells ◽  
Kinase A

CCL2 mitigates cyclic AMP‐suppressed Th2 immune response in human dendritic cells

Allergy ◽  
2020 ◽  
Vol 75 (8) ◽  
pp. 2108-2111
Author(s):  
Byoungjae Kim ◽  
Ji Woo Yeon ◽  
Ji Hyung Lee ◽  
Hyun‐Ji Lee ◽  
Junhyoung Byun ◽  
...  
Keyword(s):  
Immune Response ◽  
Dendritic Cells ◽  
Cyclic Amp ◽  
Th2 Immune Response ◽  
Human Dendritic Cells

scholarly journals Cholera Toxin and Heat-Labile Enterotoxin Activate Human Monocyte-Derived Dendritic Cells and Dominantly Inhibit Cytokine Production through a Cyclic AMP-Dependent Pathway

2002 ◽  
Vol 70 (10) ◽  
pp. 5533-5539 ◽  
Author(s):  
Kenneth C. Bagley ◽  
Sayed F. Abdelwahab ◽  
Robert G. Tuskan ◽  
Timothy R. Fouts ◽  
George K. Lewis
Keyword(s):  
Dendritic Cells ◽  
Cyclic Amp ◽  
Cholera Toxin ◽  
Human Monocyte ◽  
Cell Types ◽  
Interleukin 12 ◽  
Necrosis Factor Alpha ◽  
Heat Labile

ABSTRACT Cholera toxin (CT) and heat-labile enterotoxin (LT) are powerful mucosal adjuvants whose cellular targets and mechanism of action are unknown. There is emerging evidence that dendritic cells (DC) are one of the principal cell types that mediate the adjuvant effects of these toxins in vivo. Here we investigate the effects of CT and LT on the maturation of human monocyte-derived DC (MDDC) in vitro. We found that an enzymatically active A domain is necessary for both CT and LT to induce the maturation of MDDC and that this activation is strictly cyclic AMP (cAMP) dependent. ADP-ribosylation-defective derivatives of these toxins failed to induce maturation of MDDC, whereas dibutyryl-cyclic-3′,5′-AMP and Forskolin mimic the maturation of MDDC induced by CT and LT. In addition, an inhibitor of cAMP-dependent kinases, Rp-8-Br-cAMPs, blocked the ability of CT, LT, and Forskolin to activate MDDC. CT, LT, dibutyryl-cyclic-3′,5′-AMP, and Forskolin also dominantly inhibit interleukin 12 and tumor necrosis factor alpha production by MDDC in the presence of saturating concentrations of lipopolysaccharide. Taken together, these results show that the effects of CT and LT on MDDC are mediated by cAMP.

scholarly journals Monocyte Selectivity and Tissue Localization Suggests a Role for Breast and Kidney–Expressed Chemokine (Brak) in Macrophage Development

2001 ◽  
Vol 194 (6) ◽  
pp. 855-862 ◽  
Author(s):  
Isabel Kurth ◽  
Katharina Willimann ◽  
Patrick Schaerli ◽  
Thomas Hunziker ◽  
Ian Clark-Lewis ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cyclic Amp ◽  
Pertussis Toxin ◽  
Bordetella Pertussis ◽  
Normal Skin ◽  
Dermal Fibroblasts ◽  
Blood Leukocytes ◽  
Monocyte Chemoattractant ◽  
Skin Keratinocytes ◽  
Bordetella Pertussis Toxin

Although numerous chemokines act on monocytes, none of them is specific for these cells. Here, we show that breast and kidney–expressed chemokine (BRAK) is a highly selective monocyte chemoattractant. Migration efficacy and Bordetella pertussis toxin–sensitive Ca2+ mobilization responses to BRAK were strongly enhanced after treatment of monocytes with the cyclic AMP–elevating agents prostaglandin E2 and forskolin. BRAK is the first monocyte-selective chemokine, as other types of blood leukocytes or monocyte-derived dendritic cells and macrophages did not respond. Expression in normal skin keratinocytes and dermal fibroblasts as well as lamina propria cells in normal intestinal tissues suggests a homeostatic rather than an inflammatory function for this chemokine. In addition, macrophages were frequently found to colocalize with BRAK-producing fibroblasts. We propose that BRAK is involved in the generation of tissue macrophages by recruiting extravasated precursors to fibroblasts, which are known to secrete essential cytokines for macrophage development.

Cytochemical Evidence for Presynatic β-Adrenergic Regulation of Secretion in the Developing Rat Submandibular Gland

1977 ◽  
Vol 35 ◽  
pp. 430-431
Author(s):  
L.S. Cutler
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Submandibular Gland ◽  
Neonatal Rat ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Parotid Glands ◽  
Adrenergic Agonist ◽  
Rat Submandibular Gland

Many studies previously have shown that the B-adrenergic agonist isoproterenol and the a-adrenergic agonist norepinephrine will stimulate secretion by the adult rat submandibular (SMG) and parotid glands. Recent data from several laboratories indicates that adrenergic agonists bind to specific receptors on the secretory cell surface and stimulate membrane associated adenylate cyclase activity which generates cyclic AMP. The production of cyclic AMP apparently initiates a cascade of events which culminates in exocytosis. During recent studies in our laboratory it was observed that the adenylate cyclase activity in plasma membrane fractions derived from the prenatal and early neonatal rat submandibular gland was retractile to stimulation by isoproterenol but was stimulated by norepinephrine. In addition, in vitro secretion studies indicated that these prenatal and neonatal glands would not secrete peroxidase in response to isoproterenol but would secrete in response to norepinephrine. In contrast to these in vitro observations, it has been shown that the injection of isoproterenol into the living newborn rat results in secretion of peroxidase by the SMG (1).

Dendritic cells of the human epidermis: immuno-electron microscopic studies of la antigen reactivity

1978 ◽  
Vol 36 (2) ◽  
pp. 644-645
Author(s):  
G. Rowden ◽  
M. G. Lewis ◽  
T. M. Phillips
Keyword(s):  
Dendritic Cells ◽  
Langerhans Cells ◽  
Cell Types ◽  
Cell Type ◽  
Electron Microscopic ◽  
La Antigen ◽  
Microscopic Studies ◽  
A Cell ◽  
C3 Receptors ◽  
Antigen Reactivity

Langerhans cells of mammalian stratified squamous epithelial have proven to be an enigma since their discovery in 1868. These dendritic suprabasal cells have been considered as related to melanocytes either as effete cells, or as post divisional products. Although grafting experiments seemed to demonstrate the independence of the cell types, much confusion still exists. The presence in the epidermis of a cell type with morphological features seemingly shared by melanocytes and Langerhans cells has been especially troublesome. This so called "indeterminate", or " -dendritic cell" lacks both Langerhans cells granules and melanosomes, yet it is clearly not a keratinocyte. Suggestions have been made that it is related to either Langerhans cells or melanocyte. Recent studies have unequivocally demonstrated that Langerhans cells are independent cells with immune function. They display Fc and C3 receptors on their surface as well as la (immune region associated) antigens.

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