Understanding the mechanisms by which Reactive Oxygen Species (ROS) activate mononuclear phagocytes in inflammatory diseases of the lung.

Apocynin is a phenolic compound isolated from the plant Picrorhiza kurroa Royle ex Benth. Such a compound has been extensively investigated for its therapeutic potential in diseases involving inflammatory processes or oxidative stress due to its ability to inhibit the NADPH oxidase multienzyme complex. This complex consists of two transmembrane proteins (Nox2 and p22phox) and four cytosolic regulatory proteins (p67phox, p47phox, p40phox, and GTPase-Rac) and their activation occurs after the stimulation of phagocytic cells by the mediation of the enzyme myeloperoxidase (MPO). NADPH oxidase is the only enzyme complex that is intended for the production of superoxide anion that is precursor of highly oxidizing substances classified as reactive oxygen species (ROS). NADPH oxidase is an enzyme complex that produces superoxide anion from molecular oxygen. Ta the same time, the superoxide anion is a precursor to reactive oxygen species (ROS) catalyzed by enzymes.These oxidative species, when in excess, can induce burst, causing irreparable tissue damage. They can act by modifying the redox state of DNA, protein or lipid molecules, playing a central role in the development of chronic pathologies and various health complications. One can cite vascular problems, hyperglycemia, diabetes, hypertension, Alzheimer's disease, and cancer, among others. Apocynin, previously activated by MPO, blocks the enzyme complex and prevents the formation of these oxidative species. Therefore, the central biological function of compound is to modulate the action of NADPH oxidase, promoting a positive effect in the prevention/remediation of inflammatory diseases.

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The triggering of fibrinogen receptors on mononuclear phagocytes of healthy subjects and of a thrombasthenia Glanzmann patient promotes the generation of reactive oxygen species

Blood Coagulation & Fibrinolysis ◽

10.1097/00001721-199010000-00003 ◽

1990 ◽

Vol 1 (5) ◽

pp. 363-374 ◽

Cited By ~ 1

Author(s):

C. Trezzini ◽

L. Schalch ◽

T. W. Jungi

Keyword(s):

Reactive Oxygen Species ◽

Healthy Subjects ◽

Reactive Oxygen ◽

Mononuclear Phagocytes ◽

Oxygen Species

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Generation of reactive oxygen species by umbilical blood cells and immune status of newborns at risk of infectious inflammatory diseases

Bulletin of Experimental Biology and Medicine ◽

10.1007/s10517-006-0359-x ◽

2006 ◽

Vol 142 (3) ◽

pp. 333-337 ◽

Cited By ~ 3

Author(s):

V. G. Safronova ◽

N. K. Matveeva ◽

V. N. Mal’tseva ◽

O. E. Bondar’ ◽

N. V. Avkhacheva ◽

...

Keyword(s):

Reactive Oxygen Species ◽

At Risk ◽

Blood Cells ◽

Immune Status ◽

Inflammatory Diseases ◽

Reactive Oxygen ◽

Oxygen Species ◽

Umbilical Blood ◽

Umbilical Blood Cells

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Food-borne Lactiplantibacillus plantarum protect normal intestinal cells against inflammation by modulating reactive oxygen species and IL-23/IL-17 axis

Scientific Reports ◽

10.1038/s41598-020-73201-1 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Roberta Prete ◽

Natalia Garcia-Gonzalez ◽

Carla D. Di Mattia ◽

Aldo Corsetti ◽

Natalia Battista

Keyword(s):

Reactive Oxygen Species ◽

Inflammatory Diseases ◽

Reactive Oxygen ◽

Normal Mucosa ◽

Protective Role ◽

Intestinal Cells ◽

Oxygen Species ◽

Inflammatory Damage ◽

Food Borne ◽

Pre Treatment

Abstract Food-associated Lactiplantibacillus plantarum (Lpb. plantarum) strains, previously classified as Lactobacillus plantarum, are a promising strategy to face intestinal inflammatory diseases. Our study was aimed at clarifying the protective role of food-borne Lpb. plantarum against inflammatory damage by testing the scavenging microbial ability both in selected strains and in co-incubation with normal mucosa intestinal cells (NCM460). Here, we show that Lpb. plantarum endure high levels of induced oxidative stress through partially neutralizing reactive oxygen species (ROS), whereas they elicit their production when co-cultured with NCM460. Moreover, pre-treatment with food-borne Lpb. plantarum significantly reduce pro-inflammatory cytokines IL-17F and IL-23 levels in inflamed NCM460 cells. Our results suggest that food-vehicled Lpb. plantarum strains might reduce inflammatory response in intestinal cells by directly modulating local ROS production and by triggering the IL-23/IL-17 axis with future perspectives on health benefits in the gut derived by the consumption of functional foods enriched with selected strains.

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