scholarly journals Histone Deacetylase Inhibitors Upregulate Natriuretic Peptide Receptor‐A Gene Transcription via Histone Modifications and Sp1 Acetylation

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Prerna Kumar ◽  
Smitha Neerukonda ◽  
Vickie Nguyen ◽  
Kailash N Pandey
Keyword(s):  
Histone Deacetylase ◽  
Natriuretic Peptide ◽  
Gene Transcription ◽  
Histone Modifications ◽  
Histone Deacetylase Inhibitors ◽  
Natriuretic Peptide Receptor ◽  
Peptide Receptor ◽  
Natriuretic Peptide Receptor A

scholarly journals Regulation of natriuretic peptide receptor-A gene expression and stimulation of its guanylate cyclase activity by transcription factor Ets-1

2009 ◽  
Vol 29 (1) ◽  
pp. 57-70 ◽  
Author(s):  
Prerna Kumar ◽  
Gevoni Bolden ◽  
Kiran K. Arise ◽  
Stephen T. Krazit ◽  
Kailash N. Pandey
Keyword(s):  
Natriuretic Peptide ◽  
Gene Transcription ◽  
Guanylate Cyclase ◽  
Promoter Activity ◽  
Target Cells ◽  
Mrna Levels ◽  
Natriuretic Peptide Receptor ◽  
Peptide Receptor ◽  
Natriuretic Peptide Receptor A ◽  
Npr1 Gene

ANP (atrial natriuretic peptide) exerts its biological effects by binding to GC (guanylate cyclase)-A/NPR (natriuretic peptide receptor)-A, which generates the second messenger cGMP. The molecular mechanism mediating Npr1 (coding for GC-A/NPRA) gene regulation and expression is not well understood. The objective of the present study was to elucidate the mechanism by which Ets-1 [Ets (E twenty-six) transformation-specific sequence] contributes to the regulation of Npr1 gene transcription and expression. Chromatin immunoprecipitation and gel-shift assays confirmed the in vivo and in vitro binding of Ets-1 to the Npr1 promoter. Overexpression of Ets-1 enhanced significantly Npr1 mRNA levels, protein expression, GC activity and ANP-stimulated intracellular accumulation of cGMP in transfected cells. Depletion of endogenous Ets-1 by siRNA (small interfering RNA) dramatically decreased promoter activity by 80%. Moreover, methylation of the Npr1 promoter region (−356 to +55) reduced significantly the promoter activity and hypermethylation around the Ets-1 binding sites directly reduced Ets-1 binding to the Npr1 promoter. Collectively, the present study demonstrates that Npr1 gene transcription and GC activity of the receptor are critically controlled by Ets-1 in target cells.

Physical Mapping of the Human Connexin 40 (GJA5), Flavin-Containing Monooxygenase 5, and Natriuretic Peptide Receptor A Genes on 1q21

Genomics ◽  
1997 ◽  
Vol 39 (3) ◽  
pp. 409-411 ◽  
Author(s):  
Bruce D. Gelb ◽  
Jian Zhang ◽  
Philip D. Cotter ◽  
Irina F. Gershin ◽  
Robert J. Desnick
Keyword(s):  
Natriuretic Peptide ◽  
Physical Mapping ◽  
Natriuretic Peptide Receptor ◽  
Peptide Receptor ◽  
Connexin 40 ◽  
Natriuretic Peptide Receptor A

Localization by Photoaffinity Labeling of Natriuretic Peptide Receptor-A Binding Domain†

Biochemistry ◽  
1996 ◽  
Vol 35 (39) ◽  
pp. 12950-12956 ◽  
Author(s):  
Normand McNicoll ◽  
Jean Gagnon ◽  
Jean-Jacques Rondeau ◽  
Huy Ong ◽  
André De Léan
Keyword(s):  
Natriuretic Peptide ◽  
Photoaffinity Labeling ◽  
Binding Domain ◽  
Natriuretic Peptide Receptor ◽  
Peptide Receptor ◽  
Natriuretic Peptide Receptor A
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