Comparative study of in vitro expanded somatic stem cells from different sources (732.3)

The aim of the present study was to investigate whether a somatic stem cell derived from the salivary gland can be an efficient donor cell for pig cloning. Somatic stem cells (salivary gland progenitor cells, SGP) were isolated from the salivary gland of a 4-month old male pig (Matsumoto et al. 2004). Briefly, tissue sections of salivary gland were gently digested by collagenase/hyaluronidase and dispersed into single cells. Isolated cells (5 × 104) were cultured on a type I collagen-coated dish in William's medium E; then colonies having epithelial-like morphology were picked to establish the primary culture of SGP cells (CD49, intracellular laminin-positive) which have the potential to differentiate in vitro into hepatic and pancreatic endocrine cells after spherical body formation in vitro. SGP cells to be used as nuclear donors were cultured for 2 days under serum starvation. Fetal fibroblast (FF) cells were used as control nuclear donors. IVM oocytes were obtained from abattoir ovaries and matured in NCSU23. Donor cells were fused with the enucleated recipient oocytes by a single DC pulse of 190 V/mm for 10 μs in 0.28 M mannitol + 0.15 mM MgSO4. Reconstructed embryos were electrically activated by DC pulse of 150 V/mm for 100 μs in 0.28 M mannitol + 0.05 mM CaCl2 + 0.1 mM MgSO4 at 1–1.5 h after the NT, followed by cytochalasin B treatment for 3 h. Development of the NT embryos was assessed in vitro by fixing and staining at either 2 h post NT or after culture for 7 days in NCSU23, or in vivo by transfer to the oviducts of estrus-synchronized recipient gilts. Incidence of premature chromosome condensation was similar regardless of donor cell type. Development of the NT embryos reconstructed with SGP to the blastocyst stage was significantly higher compared to that of the FF group (38/137, 27.7% vs. 19/168, 11.3%, respectively; P < 0.05). Transfer of 278 cloned embryos reconstructed with SGP to two recipients resulted in the production of three live piglets. Production efficiency of piglets from the cloned embryos reconstructed with FF was 2/263. Based on the in vitro development of the reconstructed embryos, SGP is a promising nuclear donor cell for pig cloning; further transfer experiments are to be carried out. This study was supported by PROBRAIN.

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MicroRNA-Enriched Exosomes from Different Sources of Mesenchymal Stem Cells Can Differentially Modulate Functions of Immune Cells and Neurogenesis

Biomedicines ◽

10.3390/biomedicines10010069 ◽

2021 ◽

Vol 10 (1) ◽

pp. 69

Author(s):

Naina Soni ◽

Suchi Gupta ◽

Surender Rawat ◽

Vishnu Krishnakumar ◽

Sujata Mohanty ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Expression Profile ◽

Immune Cells ◽

Biologically Active ◽

Rna Seq ◽

Adult Mesenchymal Stem Cells ◽

Neutrophil Survival ◽

Different Sources

Adult Mesenchymal stem cells-derived exosomes carry several biologically active molecules that play prominent roles in controlling disease manifestations. The content of these exosomes, their functions, and effect on the immune cells may differ depending on their tissue sources. Therefore, in this study, we purified the exosomes from three different sources and, using the RNA-Seq approach, highly abundant microRNAs were identified and compared between exosomes and parental cells. The effects of exosomes on different immune cells were studied in vitro by incubating exosomes with PBMC and neutrophils and assessing their functions. The expression levels of several miRNAs varied within the different MSCs and exosomes. Additionally, the expression profile of most of the miRNAs was not similar to that of their respective sources. Exosomes isolated from different sources had different abilities to induce the process of neurogenesis and angiogenesis. Moreover, these exosomes demonstrated their varying effect on PBMC proliferation, neutrophil survival, and NET formation, highlighting their versatility and broad interaction with immune cells. The knowledge gained from this study will improve our understanding of the miRNA landscape of exosomes from hMSCs and provide a resource for further improving our understanding of exosome cargo and their interaction with immune cells.

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In vitro osteogenic differentiation of stem cells with different sources on composite scaffold containing natural bioceramic and polycaprolactone

Artificial Cells Nanomedicine and Biotechnology ◽

10.1080/21691401.2018.1553785 ◽

2019 ◽

Vol 47 (1) ◽

pp. 300-307 ◽

Cited By ~ 8

Author(s):

Fatemeh Sadat Hosseini ◽

Fatemeh Soleimanifar ◽

Abdolreza Ardeshirylajimi ◽

Saeid Vakilian ◽

Majid Mossahebi-Mohammadi ◽

...

Keyword(s):

Stem Cells ◽

Osteogenic Differentiation ◽

Composite Scaffold ◽

Different Sources

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In vitro differentiation of unrestricted somatic stem cells into functional hepatic-like cells displaying a hepatocyte-like glucose metabolism

Journal of Cellular Physiology ◽

10.1002/jcp.22237 ◽

2010 ◽

Vol 225 (2) ◽

pp. 545-554 ◽

Cited By ~ 23

Author(s):

Simon Waclawczyk ◽

Anja Buchheiser ◽

Ulrich Flögel ◽

Teja F. Radke ◽

Gesine Kögler

Keyword(s):

Stem Cells ◽

Glucose Metabolism ◽

In Vitro Differentiation ◽

Somatic Stem Cells ◽

Unrestricted Somatic Stem Cells

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Unrestricted somatic stem cells: interaction with CD34+ cells in vitro and in vivo, expression of homing genes and exclusion of tumorigenic potential

Cytotherapy ◽

10.3109/14653249.2010.523076 ◽

2011 ◽

Vol 13 (3) ◽

pp. 357-365 ◽

Cited By ~ 11

Author(s):

Kathrin Sonja Jeltsch ◽

Teja Falk Radke ◽

Stephanie Laufs ◽

Frank Anton Giordano ◽

Heike Allgayer ◽

...

Keyword(s):

Stem Cells ◽

Somatic Stem Cells ◽

Tumorigenic Potential ◽

Cd34 Cells ◽

In Vivo Expression ◽

Unrestricted Somatic Stem Cells

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Potential of Newborn and Adult Stem Cells for the Production of Vascular Constructs Using the Living Tissue Sheet Approach

BioMed Research International ◽

10.1155/2015/168294 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10 ◽

Cited By ~ 5

Author(s):

Jean-Michel Bourget ◽

Robert Gauvin ◽

David Duchesneau ◽

Murielle Remy ◽

François A. Auger ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Adult Stem Cells ◽

Dermal Fibroblasts ◽

Mechanical Resistance ◽

Self Organized ◽

Associated Proteins ◽

Different Sources ◽

Potential Use

Bypass surgeries using native vessels rely on the availability of autologous veins and arteries. An alternative to those vessels could be tissue-engineered vascular constructs made by self-organized tissue sheets. This paper intends to evaluate the potential use of mesenchymal stem cells (MSCs) isolated from two different sources: (1) bone marrow-derived MSCs and (2) umbilical cord blood-derived MSCs. When culturedin vitro, a proportion of those cells differentiated into smooth muscle cell- (SMC-) like cells and expressed contraction associated proteins. Moreover, these cells assembled into manipulable tissue sheets when cultured in presence of ascorbic acid. Tubular vessels were then produced by rolling those tissue sheets on a mandrel. The architecture, contractility, and mechanical resistance of reconstructed vessels were compared with tissue-engineered media and adventitia produced from SMCs and dermal fibroblasts, respectively. Histology revealed a collagenous extracellular matrix and the contractile responses measured for these vessels were stronger than dermal fibroblasts derived constructs although weaker than SMCs-derived constructs. The burst pressure of bone marrow-derived vessels was higher than SMCs-derived ones. These results reinforce the versatility of the self-organization approach since they demonstrate that it is possible to recapitulate a contractile media layer from MSCs without the need of exogenous scaffolding material.

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Comparative study of in vitro expansion of bone marrow-derived mesenchymal stem cells

Biotechnology Letters ◽

10.1007/s10529-012-1083-4 ◽

2012 ◽

Vol 35 (3) ◽

pp. 463-469 ◽

Cited By ~ 2

Author(s):

Stefan Peter ◽

Andy M. Scutt ◽

Phillip C. Wright ◽

Catherine A. Biggs

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Comparative Study ◽

In Vitro Expansion

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In VitroandIn VivoHepatic Differentiation of Adult Somatic Stem Cells and Extraembryonic Stem Cells for Treating End Stage Liver Diseases

Stem Cells International ◽

10.1155/2015/871972 ◽

2015 ◽

Vol 2015 ◽

pp. 1-11 ◽

Cited By ~ 20

Author(s):

Chenxia Hu ◽

Lanjuan Li

Keyword(s):

Stem Cells ◽

Liver Tissue ◽

Liver Diseases ◽

Embryonic Stem ◽

Somatic Stem Cells ◽

Hepatic Differentiation ◽

Short Supply ◽

End Stage ◽

Promising Source

The shortage of liver donors is a major handicap that prevents most patients from receiving liver transplantation and places them on a waiting list for donated liver tissue. Then, primary hepatocyte transplantation and bioartificial livers have emerged as two alternative treatments for these often fatal diseases. However, another problem has emerged. Functional hepatocytes for liver regeneration are in short supply, and they will dedifferentiate immediatelyin vitroafter they are isolated from liver tissue. Alternative stem-cell-based therapeutic strategies, including hepatic stem cells (HSCs), embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and mesenchymal stem cells (MSCs), are more promising, and more attention has been devoted to these approaches because of the high potency and proliferation ability of the cells. This review will focus on the general characteristics and the progress in hepatic differentiation of adult somatic stem cells and extraembryonic stem cellsin vitroandin vivofor the treatment of end stage liver diseases. The hepatic differentiation of stem cells would offer an ideal and promising source for cell therapy and tissue engineering for treating liver diseases.

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