scholarly journals Effect of Interleukin‐10 and Laminar Shear Stress on African American Human Umbilical Vein Endothelial Cells

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Dianne Babbitt ◽  
Ji‐Seok Kim ◽  
Steven Forrester ◽  
Michael Brown ◽  
Joon‐Young Park
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
African American ◽  
Umbilical Vein ◽  
Interleukin 10 ◽  
Laminar Shear Stress ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells ◽  
Laminar Shear

Effects of Laminar Shear Stress on Mitochondrial Biogenesis in Human Umbilical Vein Endothelial Cells

2011 ◽  
Vol 43 (Suppl 1) ◽  
pp. 464
Author(s):  
BOA KIM ◽  
Ji-Seok Kim ◽  
Deborah L. Feairheller ◽  
Kathleen M. Sturgeon ◽  
Michael D. Brown ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Mitochondrial Biogenesis ◽  
Umbilical Vein ◽  
Laminar Shear Stress ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells ◽  
Laminar Shear

scholarly journals Primary cilia of human endothelial cells disassemble under laminar shear stress

2004 ◽  
Vol 164 (6) ◽  
pp. 811-817 ◽  
Author(s):  
Carlo Iomini ◽  
Karla Tejada ◽  
Wenjun Mo ◽  
Heikki Vaananen ◽  
Gianni Piperno
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Primary Cilia ◽  
Umbilical Vein ◽  
Intraflagellar Transport ◽  
Mechanical Stimuli ◽  
Laminar Shear Stress ◽  
Human Endothelial Cells ◽  
Umbilical Vein Endothelial Cells ◽  
Laminar Shear

We identified primary cilia and centrosomes in cultured human umbilical vein endothelial cells (HUVEC) by antibodies to acetyl-α-tubulin and capillary morphogenesis gene-1 product (CMG-1), a human homologue of the intraflagellar transport (IFT) protein IFT-71 in Chlamydomonas. CMG-1 was present in particles along primary cilia of HUVEC at interphase and around the oldest basal body/centriole at interphase and mitosis. To study the response of primary cilia and centrosomes to mechanical stimuli, we exposed cultured HUVEC to laminar shear stress (LSS). Under LSS, all primary cilia disassembled, and centrosomes were deprived of CMG-1. We conclude that the exposure to LSS ends the IFT in cultured endothelial cells.

scholarly journals Effect of Interleukin-10 and Laminar Shear Stress on Endothelial Nitric Oxide Synthase and Nitric Oxide in African American Human Umbilical Vein Endothelial Cells

2015 ◽  
Vol 25 (4) ◽  
pp. 413 ◽  
Author(s):  
Dianne M. Babbitt ◽  
Ji-Seok Kim ◽  
Steven J. Forrester ◽  
Michael D. Brown ◽  
Joon-Young Park
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
African American ◽  
Umbilical Vein ◽  
Interleukin 10 ◽  
Laminar Shear Stress ◽  
Experimental Conditions ◽  
Umbilical Vein Endothelial Cells ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

<p><strong>Background: </strong>African Americans have a pre­disposition to heightened systemic inflamma­tion and a high prevalence of hypertension.</p><p><strong>Objective: </strong>The purpose of this study was to evaluate the influence of interleukin-10 (IL- 10) and laminar shear stress (LSS) on African American endothelial cells by measuring to­tal endothelial nitric oxide synthase (eNOS) protein expression and its phosphorylated form (p-eNOS) at Serine 1177, and nitric oxide (NO) levels, in response to IL-10 incubation and high physiological levels of LSS, used as an <em>in vitro </em>mimetic for aerobic exercise training (AEXT).</p><p><strong>Design: </strong>Human umbilical vein endothelial cells (HUVEC) from an African American donor were cultured. The experimental conditions included <em>Static</em>, <em>Static with IL-10 Incubation, LSS at 20 dynes/cm</em><em>2</em><em>, and LSS at 20 dynes/cm</em><em>2 </em><em>with IL-10 Incubation</em>. West­ern blotting was used to measure eNOS and p-eNOS protein expression in the cells. A modified Griess assay was used to measure NO metabolites in the cell culture media.</p><p><strong>Results: </strong>There were significant increases in p-eNOS, eNOS, and NO in the <em>LSS at 20 dynes/cm</em><em>2 </em>and <em>LSS at 20 dynes/cm</em><em>2 </em><em>with IL-10 Incubation </em>experimental conditions when compared to the <em>Static </em>experimental condition. There were no other statistically significant differences demonstrating that IL- 10 did not have an additive effect on eNOS activity in our study.</p><p><strong>Conclusion: </strong>The significant increases in p-eNOS, eNOS, and NO as a result of LSS in African American HUVECs suggest that AEXT may be a viable, nonpharmacologic method to improve vascular inflamma­tion status and vasodilation, and thereby contribute to hypertension reduction in the African American population. <em>Ethn Dis. </em>2015;25(4):413-418; doi:10.18865/ ed.25.4.413</p>

Gradients in cytoplasmic calcium concentration ([Ca2+]i) in migrating human umbilical vein endothelial cells (HUVECs) stimulated by shear-stress

Life Sciences ◽  
1999 ◽  
Vol 65 (24) ◽  
pp. 2643-2651 ◽  
Author(s):  
Norihide Yoshikawa ◽  
Hideo Ariyoshi ◽  
Yasuhisa Aono ◽  
Masato Sakon ◽  
Tomio Kawasaki ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Calcium Concentration ◽  
Umbilical Vein ◽  
Cytoplasmic Calcium ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells
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