scholarly journals Low Shear Stress Regulating Autophagy Mediated by the p38 Mitogen Activated Protein Kinase and p53 Pathways in Human Umbilical Vein Endothelial Cells

2018 ◽  
Vol 131 (9) ◽  
pp. 1132-1133 ◽  
Author(s):  
Hui-Zhen Liu ◽  
Li Li ◽  
Shao-Liang Chen ◽  
Jian-Rui Wei ◽  
Jun-Xia Zhang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Protein Kinase ◽  
Umbilical Vein ◽  
Mitogen Activated Protein Kinase ◽  
Human Umbilical Vein ◽  
Mitogen Activated Protein ◽  
Low Shear Stress ◽  
Umbilical Vein Endothelial Cells ◽  
Low Shear

Angiotensin II effect on calcium mobilization and mitogen-activated protein kinase activation in human umbilical vein endothelial cells

2003 ◽  
Vol 3 (56) ◽  
pp. 201-208 ◽  
Author(s):  
Mercedes Montiel ◽  
M. Idoia Gámez ◽  
J. Jesús García-Vallejo ◽  
Enrique Pérez de la Blanca ◽  
Elisa Martín ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Angiotensin Ii ◽  
Protein Kinase ◽  
Umbilical Vein ◽  
Mitogen Activated Protein Kinase ◽  
Kinase Activation ◽  
Human Umbilical Vein ◽  
Protein Kinase Activation ◽  
Mitogen Activated Protein ◽  
Umbilical Vein Endothelial Cells

Thrombin induces increased expression and secretion of angiopoietin-2 from human umbilical vein endothelial cells

Blood ◽  
2002 ◽  
Vol 99 (5) ◽  
pp. 1646-1650 ◽  
Author(s):  
Yao-Qi Huang ◽  
Jian-Jun Li ◽  
Liang Hu ◽  
Merlin Lee ◽  
Simon Karpatkin
Keyword(s):  
Endothelial Cells ◽  
Protein Synthesis ◽  
Messenger Rna ◽  
Umbilical Vein ◽  
Mitogen Activated Protein Kinase ◽  
Northern Analysis ◽  
Human Umbilical Vein ◽  
Mitogen Activated Protein ◽  
Umbilical Vein Endothelial Cells ◽  
Angiopoietin 2

Angiogenesis is required for tumor growth and metastasis. It has recently been suggested that thrombin is a potent promoter of angiogenesis. We therefore examined the possibility that thrombin could be inducing the expression of angiopoietin-2 (Ang-2), necessary for remodeling. Human umbilical vein endothelial cells were incubated with or without thrombin (1 U/mL) for 1 to 24 hours and then examined for messenger RNA (mRNA) by Northern analysis. Enhanced mRNA expression (about 4-fold over baseline) was noted at 4 hours. Enhanced expression of Ang-2 mRNA was secondary to enhanced transcription (about 4-fold), with no effect on stabilization. Enhanced Ang-2 mRNA transcription was inhibited by H7 and PD98059, indicating the requirement of serine/threonine kinases as well as the mitogen-activated protein kinase pathway. Up-regulation of mRNA was associated with enhanced Ang-2 protein synthesis and secretion as assayed by immunoblot. Thrombin-induced secreted Ang-2 inhibited the binding of recombinant 35S–Ang-1 to its Tie-2–Fc receptor, demonstrating functionality. Hirudin reversed this effect, demonstrating thrombin specificity. Thus, thrombin-induced tumorigenesis and metastasis is associated with enhanced Ang-2 protein synthesis and secretion via enhanced transcription of Ang-2. This could help explain how thrombin promotes angiogenesis.

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