Fluorescent Nanodiamonds for Detecting Free-Radical Generation in Real Time during Shear Stress in Human Umbilical Vein Endothelial Cells

ACS Sensors ◽  
2021 ◽  
Author(s):  
Rokshana Sharmin ◽  
Thamir Hamoh ◽  
Alina Sigaeva ◽  
Aldona Mzyk ◽  
Viraj G. Damle ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Free Radical ◽  
Real Time ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Free Radical Generation ◽  
Fluorescent Nanodiamonds ◽  
Radical Generation ◽  
Umbilical Vein Endothelial Cells

Measurement and characterization of free radical generation in reoxygenated human endothelial cells

1994 ◽  
Vol 266 (3) ◽  
pp. C700-C708 ◽  
Author(s):  
J. L. Zweier ◽  
P. Kuppusamy ◽  
S. Thompson-Gorman ◽  
D. Klunk ◽  
G. A. Lutty
Keyword(s):  
Endothelial Cells ◽  
Free Radical ◽  
Umbilical Vein ◽  
Human Endothelial Cells ◽  
Paramagnetic Resonance ◽  
Free Radical Generation ◽  
Tissue Ischemia ◽  
Radical Generation ◽  
Umbilical Vein Endothelial Cells

The endothelial cell is thought to be an important site of free radical generation in ischemic tissues. It has been demonstrated that endothelial cells from several species generate a burst of free radical generation upon reoxygenation; however, it has been suggested that human endothelial cells are not similarly capable of generating free radicals on reoxygenation. In view of the central importance of revascularization with accompanying reoxygenation in the clinical treatment of tissue ischemia/infarction, we have performed studies to determine the presence, mechanism, and kinetics of free radical generation in human endothelial cells. Therefore, we subjected cultured human umbilical vein endothelial cells to anoxia followed by reoxygenation. Cell suspensions of 10(7) cells/ml were subjected to varying periods of anoxia and reoxygenation. On reoxygenation with addition of a 50 mM concentration of the spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), after 90 min of anoxia an electron paramagnetic resonance (EPR) signal was observed consisting of 2 components: a quartet 1:2:2:1 DMPO-OH signal, aN = aH = 14.9 G, and a six-peaked DMPO-R signal, aN = 15.6 G aH = 22.9 G, whereas cells in air gave no signal. The observed signal was quenched by superoxide dismutase (SOD) or catalase. Deferoxamine decreased the measured radical signals by 40%. Cyclooxygenase blockers did not decrease radical generation, but the xanthine oxidase blocker oxypurinol did decrease radical generation by 60%.

Gradients in cytoplasmic calcium concentration ([Ca2+]i) in migrating human umbilical vein endothelial cells (HUVECs) stimulated by shear-stress

Life Sciences ◽  
1999 ◽  
Vol 65 (24) ◽  
pp. 2643-2651 ◽  
Author(s):  
Norihide Yoshikawa ◽  
Hideo Ariyoshi ◽  
Yasuhisa Aono ◽  
Masato Sakon ◽  
Tomio Kawasaki ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Calcium Concentration ◽  
Umbilical Vein ◽  
Cytoplasmic Calcium ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells
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