In vitro production of type 1 and type 2 cytokines by peripheral blood mononuclear cells from high-risk HIV-negative intravenous drug users

AIDS ◽  
1995 ◽  
Vol 9 (7) ◽  
pp. 691-694 ◽  
Author(s):  
Wilma Barcellini ◽  
Gian Paolo Rizzardi ◽  
Claudio Velati ◽  
Maria Orietta Borghi ◽  
Cristina Fain ◽  
...  
Keyword(s):  
Drug Users ◽  
Mononuclear Cells ◽  
In Vitro Production ◽  
Peripheral Blood Mononuclear ◽  
Type 2 Cytokines ◽  
Vitro Production ◽  
Hiv Negative

scholarly journals Effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patients

2006 ◽  
Vol 124 (4) ◽  
pp. 219-222 ◽  
Author(s):  
Maria Cristina Foss-Freitas ◽  
Norma Tiraboschi Foss ◽  
Eduardo Antonio Donadi ◽  
Milton Cesar Foss
Keyword(s):  
Metabolic Control ◽  
Mononuclear Cells ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
In Vitro Proliferation ◽  
Peripheral Blood Mononuclear ◽  
Type 2 Diabetic

CONTEXT AND OBJECTIVE: Diabetes mellitus is a clinical syndrome that frequently leads to the development of chronic complications and high susceptibility to infections. It is probably due to defective immunological defense, which may be related to metabolic control of the disease. The aim of this study was to evaluate the effect of metabolic control on immune-cell behavior in type 1 and type 2 diabetic patients. For this, the in vitro proliferation of peripheral blood mononuclear cells (PBMC) was analyzed in patients with inadequate and adequate metabolic control. DESIGN AND SETTING: Experimental/laboratory study at a university hospital. METHODS: Eleven type 1 and thirteen type 2 diabetic patients were studied, together with 21 healthy individuals divided in two groups (11/10), who were matched by sex and age with those diabetic patients. PBMC cultures stimulated with concanavalin-A (Con-A) were used to measure ³H-thymidine incorporation after 72 hours of cell culturing. For patients with inadequate metabolic control, culturing was performed on the first day of patient hospitalization and again after intensive treatment to achieve adequate control. RESULTS: The proliferation index for Con-A-stimulated cultures from type 1 diabetic patients was significantly greater than that for cultures from healthy individuals and type 2 diabetic patients, independent of metabolic control. A negative correlation between the proliferation cell index and body mass index and serum C-reactive protein levels was also observed. CONCLUSION: The increase in the proliferation capacity of type 1 diabetic T lymphocytes was probably not caused by hyperglycemia and/or insulinopenia related to inadequate metabolic control.

scholarly journals Inflammatory cytokines in vitro production are associated with Ala16Val superoxide dismutase gene polymorphism of peripheral blood mononuclear cells

Cytokine ◽  
2012 ◽  
Vol 60 (1) ◽  
pp. 30-33 ◽  
Author(s):  
Marco Aurélio Echart Montano ◽  
Ivana Beatrice Mânica da Cruz ◽  
Marta Maria Medeiros Frescura Duarte ◽  
Cristina da Costa Krewer ◽  
Maria Izabel de Ugalde Marques da Rocha ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Gene Polymorphism ◽  
Inflammatory Cytokines ◽  
Mononuclear Cells ◽  
In Vitro Production ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Superoxide Dismutase Gene ◽  
Vitro Production
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