DIHYDROPYRIDINES INFLUENCE INTRACELLULAR SIGNALING IN VASCULAR SMOOTH MUSCLE CELLS BY INTERFERING DIRECTLY WITH TRANSLOCATION AND MEMBRANE BINDING OF PKC

2000 ◽  
Vol 18 ◽  
pp. S10
Author(s):  
H. Haller ◽  
C. Maasch ◽  
C. Lindschau ◽  
F. C. Luft
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Intracellular Signaling ◽  
Membrane Binding ◽  
Muscle Cells

Micro-RNA Regulation of Vascular Smooth Muscle Cells and Its Significance in Cardiovascular Diseases

2021 ◽  
Author(s):  
Duong Ngoc Diem Nguyen ◽  
William M Chilian ◽  
Shamsul Mohd Zain ◽  
Muhammad Fauzi Daud ◽  
Yuh Fen Pung
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Intracellular Signaling ◽  
Neointimal Hyperplasia ◽  
Muscle Cells ◽  
Micro Rna ◽  
Phenotypic Switching ◽  
Micro Rnas

Cardiovascular disease (CVD) is among the leading causes of death worldwide. Micro-RNAs (miRNAs), regulatory molecules that repress protein expression, have attracted considerable attention in CVD research. The vasculature plays a big role in CVD development and progression and dysregulation of vascular cells underlies the root of many vascular diseases. This review provides a brief introduction of the biogenesis of miRNAs and exosomes, followed by overview of the regulatory mechanisms of miRNAs in vascular smooth muscle cells (VSMCs) intracellular signaling during phenotypic switching, senescence, calcification and neointimal hyperplasia. Evidence of extracellular signaling of VSMCs and other cells via exosomal and circulating miRNAs was also presented. Lastly, current drawbacks and limitations of miRNA studies in CVD research and potential ways to overcome these disadvantages were discussed in detail. In-depth understanding of VSMC regulation via miRNAs will add substantial knowledge and advance research in diagnosis, disease progression and/or miRNA-derived therapeutic approaches in CVD research.

scholarly journals Intracellular Signaling of Angiotensin II-induced p70 S6 Kinase Phosphorylation at Ser411in Vascular Smooth Muscle Cells

1999 ◽  
Vol 274 (52) ◽  
pp. 36843-36851 ◽  
Author(s):  
Satoru Eguchi ◽  
Hiroaki Iwasaki ◽  
Hikaru Ueno ◽  
Gerald D. Frank ◽  
Evangeline D. Motley ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Intracellular Signaling ◽  
Muscle Cells ◽  
S6 Kinase ◽  
P70 S6 Kinase ◽  
Kinase Phosphorylation

scholarly journals Cell shape regulates subcellular organelle location to control early Ca2+ signal dynamics in vascular smooth muscle cells

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
R. C. Calizo ◽  
M. K. Bell ◽  
A. Ron ◽  
M. Hu ◽  
S. Bhattacharya ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Plasma Membrane ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Intracellular Signaling ◽  
Cell Shape ◽  
Muscle Cells ◽  
Phenotypic Changes ◽  
Subcellular Organization

Abstract The shape of the cell is connected to its function; however, we do not fully understand underlying mechanisms by which global shape regulates a cell’s functional capabilities. Using theory, experiments and simulation, we investigated how physiologically relevant cell shape changes affect subcellular organization, and consequently intracellular signaling, to control information flow needed for phenotypic function. Vascular smooth muscle cells going from a proliferative and motile circular shape to a contractile fusiform shape show changes in the location of the sarcoplasmic reticulum, inter-organelle distances, and differential distribution of receptors in the plasma membrane. These factors together lead to the modulation of signals transduced by the M3 muscarinic receptor/Gq/PLCβ pathway at the plasma membrane, amplifying Ca2+ dynamics in the cytoplasm, and the nucleus resulting in phenotypic changes, as determined by increased activity of myosin light chain kinase in the cytoplasm and enhanced nuclear localization of the transcription factor NFAT. Taken together, our observations show a systems level phenomenon whereby global cell shape affects subcellular organization to modulate signaling that enables phenotypic changes.

scholarly journals Cell shape regulates subcellular organelle location to control early Ca2+ signal dynamics in Vascular Smooth Muscle Cells

2017 ◽  
Author(s):  
R. C. Calizo ◽  
M. K. Bell ◽  
A. Ron ◽  
M. Hu ◽  
S. Bhattacharya ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Plasma Membrane ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Intracellular Signaling ◽  
Cell Shape ◽  
Muscle Cells ◽  
Phenotypic Changes ◽  
Subcellular Organization

ABSTRACTThe shape of the cell is connected to its function; however, we do not fully understand underlying mechanisms by which global shape regulates a cell’s functional capabilities. Using theory, experiments and simulation, we investigated how physiologically relevant cell shape changes affect subcellular organization, and consequently intracellular signaling, to control information flow needed for phenotypic function. Vascular smooth muscle cells going from a proliferative and motile circular shape to a contractile fusiform shape show changes in the location of the sarcoplasmic reticulum, inter-organelle distances and differential distribution of receptors in the plasma membrane. These factors together lead to the modulation of signals transduced by the M3 muscarinic receptor/Gq/PLCβ pathway at the plasma membrane, amplifying Ca2+ dynamics in the cytoplasm and the nucleus resulting in phenotypic changes, as determined by increased activity of myosin light chain kinase in the cytoplasm and enhanced nuclear localization of the transcription factor NFAT. Taken together, our observations show a systems level phenomenon whereby global cell shape affects subcellular organization to modulate signaling that enables phenotypic changes.

Evidence for functionally active protease-activated receptor-3 (PAR-3) in human vascular smooth muscle cells

2003 ◽  
Vol 90 (10) ◽  
pp. 704-709 ◽  
Author(s):  
Rainer Spanbroek ◽  
Katharina Lötzer ◽  
Andreas Richard Habenicht ◽  
Karsten Schrör ◽  
Ellen Bretschneider
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Intracellular Signaling ◽  
Muscle Cells ◽  
Calcium Signal ◽  
Extracellular Signal ◽  
Active Protease ◽  
Cellular Dna

SummaryThe present study investigates whether vascular smooth muscle cells of the human saphenous vein (SMC) express a functionally active protease-activated receptor-3 (PAR-3). PAR-3 mRNA was detected by RT-PCR. In the presence of thrombin, a rapid and transient increase in PAR-3 mRNA was observed. Stimulation of SMC with thrombin or the synthetic PAR-3-activating peptide, TFRGAP, resulted in transient mobilization of intracellular calcium. After a preceding challenge with thrombin, the calcium signal to TFRGAP was abolished, suggesting cleavage and subsequent desensitization of PAR-3 by thrombin. Activation of PAR-3 by TFRGAP elicited a time-dependent activation of the extracellular-signal-regulated kinase (ERK)-1/2 with a maximum response 10-20 min after stimulation. At 200 µM, TFRGAP increased 3H]-thymidine incorporation into cellular DNA about two-fold. These data indicate that PAR-3 is expressed in human SMC and triggers intracellular signaling. Thus, in the SMC PAR-3 might contribute to thrombin-induced responses.

Sign in / Sign up

Export Citation Format

Share Document