Factors controlling smooth muscle proliferation and airway remodelling

2002 ◽  
Vol 2 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Judith L. Black ◽  
Peter R.A. Johnson
Keyword(s):  
Smooth Muscle ◽  
Airway Remodelling ◽  
Smooth Muscle Proliferation

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

Smooth Muscle Proliferation in Chronically Injured Canine Pulmonary Arteries Is Reduced by a Potent Platelet Aggregation Inhibitor U-53,059

1985 ◽  
Vol 53 (03) ◽  
pp. 351-355 ◽  
Author(s):  
Robert G Schaub ◽  
James C Keith ◽  
Carol A Simmons ◽  
Clarence A Rawlings
Keyword(s):  
Smooth Muscle ◽  
Platelet Aggregation ◽  
Dirofilaria Immitis ◽  
Leukocyte Adhesion ◽  
Pulmonary Arteries ◽  
Cell Loss ◽  
Endothelial Cell Loss ◽  
Dense Granules ◽  
Smooth Muscle Proliferation ◽  
Aggregation Inhibitor

Summary Dirofilaria immitis (DI) infection chronically injures canine pulmonary arteries. This injury produces endothelial cell loss, platelet/leukocyte adhesion, and smooth muscle proliferation. In the present study we assessed the effect of the cyclooxygenase inhibitor, U-53,059, on platelet function, platelet kinetics, coagulation, and smooth muscle proliferation in DI infected dogs.Platelet aggregation to the combination of arachidonic acid/ ADP was significantly inhibited by U-53,059. Coagulation and hematologic parameters were not effected by either DI infection or U-53,059 treatment. Platelet survival and the number of platelet dense granules were reduced in DI infection. Quantification of the lesions demonstrated that U-53,059 reduced both severity and density compared to non-treated dogs. U-53,059 is a potent and effective inhibitor of platelet aggregation which modifies smooth muscle proliferation produced by chronic vascular injury.

scholarly journals Complementary vasoactivity and matrix remodelling in arterial adaptations to altered flow and pressure

2008 ◽  
Vol 6 (32) ◽  
pp. 293-306 ◽  
Author(s):  
A Valentín ◽  
L Cardamone ◽  
S Baek ◽  
J.D Humphrey
Keyword(s):  
Smooth Muscle ◽  
Continuum Theory ◽  
Muscle Contractility ◽  
Smooth Muscle Contractility ◽  
Rates Of Change ◽  
Biomechanical Loading ◽  
Smooth Muscle Proliferation ◽  
And Function ◽  
Diverse Data ◽  
Induced Changes

Arteries exhibit a remarkable ability to adapt to sustained alterations in biomechanical loading, probably via mechanisms that are similarly involved in many arterial pathologies and responses to treatment. Of particular note, diverse data suggest that cell and matrix turnover within vasoaltered states enables arteries to adapt to sustained changes in blood flow and pressure. The goal herein is to show explicitly how altered smooth muscle contractility and matrix growth and remodelling work together to adapt the geometry, structure, stiffness and function of a representative basilar artery. Towards this end, we employ a continuum theory of constrained mixtures to model evolving changes in the wall, which depend on both wall shear stress-induced changes in vasoactive molecules (which alter smooth muscle proliferation and synthesis of matrix) and intramural stress-induced changes in growth factors (which alter cell and matrix turnover). Simulations show, for example, that such considerations help explain the different rates of experimentally observed adaptations to increased versus decreased flows as well as differences in rates of change in response to increased flows or pressures.

scholarly journals The geranyl acetophenone tHGA attenuates human bronchial smooth muscle proliferation via inhibition of AKT phosphorylation

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Hui Min Yap ◽  
Yu Zhao Lee ◽  
Hanis Hazeera Harith ◽  
Chau Ling Tham ◽  
Manraj Singh Cheema ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Bronchial Smooth Muscle ◽  
Akt Phosphorylation ◽  
Smooth Muscle Proliferation

Rosiglitazone reduces serum homocysteine levels, smooth muscle proliferation, and intimal hyperplasia in Sprague-Dawley rats fed a high methionine diet

Metabolism ◽  
2005 ◽  
Vol 54 (5) ◽  
pp. 645-652 ◽  
Author(s):  
Subramanyam N. Murthy ◽  
Demian F. Obregon ◽  
Natasha N. Chattergoon ◽  
Neil A. Fonseca ◽  
Debasis Mondal ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Intimal Hyperplasia ◽  
Sprague Dawley ◽  
Serum Homocysteine ◽  
Sprague Dawley Rats ◽  
Smooth Muscle Proliferation

scholarly journals Poldip2 knockdown inhibits vascular smooth muscle proliferation and neointima formation by regulating the expression of PCNA and p21

2018 ◽  
Vol 99 (3) ◽  
pp. 387-398 ◽  
Author(s):  
Srinivasa Raju Datla ◽  
Lula L. Hilenski ◽  
Bonnie Seidel-Rogol ◽  
Anna E. Dikalova ◽  
Mark Harousseau ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Neointima Formation ◽  
Smooth Muscle Proliferation

Autocrine production of matrix metalloproteinase-2 is required for human airway smooth muscle proliferation

1999 ◽  
Vol 277 (6) ◽  
pp. L1109-L1117 ◽  
Author(s):  
Simon Johnson ◽  
Alan Knox
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Airway Smooth Muscle ◽  
Fetal Bovine Serum ◽  
Platelet Derived Growth Factor ◽  
Airway Smooth Muscle Cells ◽  
Human Airway Smooth Muscle ◽  
Human Airway ◽  
Smooth Muscle Proliferation ◽  
Fetal Bovine

Airway smooth muscle proliferation is important in asthma and is dependent on pro- and antimitogenic factors and cell-matrix interactions. Here we show an antiproliferative effect of protease inhibitors on human airway smooth muscle due to inhibition of autocrine-derived matrix metalloproteinase (MMP)-2. Proliferation in response to fetal bovine serum, thrombin, and platelet-derived growth factor was inhibited by the broad-spectrum protease inhibitor Complete and the MMP inhibitors EDTA and Ro-31-9790 but not by cysteine or serine protease inhibitors. Conditioned medium from airway smooth muscle cells contained 72-kDa gelatinase that was secreted by growth-arrested cells and increased by fetal bovine serum but not by thrombin or platelet-derived growth factor. Immunostaining of cultured human airway smooth muscle cells and normal lung biopsies confirmed this gelatinase to be MMP-2. Our results suggest a novel role for MMP-2 as an important autocrine factor required for airway smooth muscle proliferation. Inhibition of MMPs could provide a target for the prevention of smooth muscle hyperplasia and airway remodeling in asthma.

scholarly journals Loss of Spry1 attenuates vascular smooth muscle proliferation by impairing mitogen‐mediated changes in cell cycle regulatory circuits

2017 ◽  
Vol 119 (4) ◽  
pp. 3267-3279 ◽  
Author(s):  
Xuehui Yang ◽  
Yan Gong ◽  
Qing He ◽  
Jonathan D. Licht ◽  
Lucy Liaw ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Regulatory Circuits ◽  
Smooth Muscle Proliferation
Sign in / Sign up

Export Citation Format

Share Document