The Role of Environmental Changes on Monospecies Biofilm Formation on Root Canal Wall by Enterococcus faecalis

2005 ◽  
Vol 31 (12) ◽  
pp. 867-872 ◽  
Author(s):  
S. George ◽  
A. Kishen ◽  
P. Song
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Biofilm Formation ◽  
Environmental Changes ◽  
Canal Wall

scholarly journals Role of extracellular DNA in Enterococcus faecalis biofilm formation and its susceptibility to sodium hypochlorite

2019 ◽  
Vol 27 ◽  
Author(s):  
Mi-Kyung YU ◽  
Mi-Ah KIM ◽  
Vinicius ROSA ◽  
Yun-Chan HWANG ◽  
Massimo DEL FABBRO ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Biofilm Formation ◽  
Extracellular Dna

scholarly journals Pathogenicity Biofilm formation of Enterococcus faecalis

2020 ◽  
Vol 5 (1) ◽  
pp. 47-50
Author(s):  
Nur Asmah
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Biofilm Formation ◽  
Laboratory Diagnosis ◽  
Toxin Production ◽  
Limited Information ◽  
Periapical Lesions ◽  
Alkaline Conditions ◽  
Autolytic Activity ◽  
Acid Reaction

Biofilm formation is closely related to the pathogenicity of E. faecalis in persistent root canal infections. Limited information is available about the ability and characteristics of E. faecalis biofilm-forming in the persistent pathogenicity of root canal infections. Based on these problems, the purpose of this paper is to improve the molecular understanding of E. faecalis on virulence factors associated with biofilm formation against persistent root canal infections to support laboratory diagnosis and therapy of oral E. faecalis. In conclusion, the release of cytokines triggers the dlt gene of LTA to improving: homeostasis, autolytic activity, and bacterial envelope properties. The role of cytolysin activated by the cylLL and cylLS genes improving the survival ability of E. Faecalis. Hyaluronidase will facilitate other bacteria to migrate from the root canal to periapical lesions resulting in the triggering of toxin production, which will increase damage and inflammation in the host. Enterococcus faecalis, through dentine matrix formation, hydrolyze collagen and induce apatite deposition in developing biofilms. Besides, these bacteria can also increase their tolerance to antimicrobials by blocking the inflammatory response's acid reaction. Alkaline conditions will neutralize the lactic acid secreted by osteoclasts to absorb hard tissue

scholarly journals CRISPR-cas system in the acquisition of virulence genes in dental-root canal and hospital-acquired isolates of Enterococcus faecalis

2019 ◽  
Author(s):  
Pourya Gholizadeh ◽  
Mohammad Aghazadeh ◽  
Reza Ghotaslou ◽  
Mohammad Ahangarzadeh Rezaee ◽  
Tahereh Pirzadeh ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Biofilm Formation ◽  
Virulence Genes ◽  
Virulence Determinants ◽  
Root Canals ◽  
Causative Agents ◽  
Life Threatening ◽  
Selective Forces ◽  
Hospital Acquired

Abstract Introduction Enterococcus faecalis is one of the important causative agents of nosocomial and life-threatening infections in human. Several studies have demonstrated that the presence of CRISPR- cas is associated with antibiotic susceptibility and lack of virulence traits. In this study, we aimed to assess the phenotypic and genotypic virulence determinants in relation to CRISPR elements from the dental-root canals and hospital-acquired isolates of E. faecalis .Methods and materials Eighty-eight hospital-acquired and 73 dental-root canal isolates of E. faecalis were assessed in this study. Phenotypic screening of the isolates included biofilm formation, and gelatinase and hemolysis activities. Genotypical screening using PCR was further used to evaluate the presence of CRISPR elements and different virulence-associated genes such as efaA , esp , cylA , hyl , gelE , ace , ebpR , and asa1 .Results Biofilm formation, and gelatinase and hemolysis activity were detected in 93.8%, 29.2% and 19.2% of the isolates, respectively. The most prevalent virulence-associated gene was ace , which was followed by efaA , whereas cylA was the least identified. The presence of CRISPR1- cas , orphan CRISPR2 and CRISPR3- cas was determined in 13%, 55.3% and 17.4% of the isolates, respectively. CRISPR elements were significantly more prevalent in the dental-root canals isolates. An inverse significant correlation was found between CRISPR- cas loci, esp and gelE , while direct correlations were observed in the case of cylA , hyl , gelE (among CRISPR-loci 1 and 3), asa1 , ace , biofilm formation, and hemolysis activity.Conclusion Findings, therefore, indicate that CRISPR- cas might prevent the acquisition of some respective pathogenicity factors in some isolates, though not all; so selective forces could not influence pathogenic traits.

scholarly journals Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating Materials

2019 ◽  
Author(s):  
Behnam Bolhari ◽  
Abbas Bahador ◽  
Mehrfam Khoshkhounejad ◽  
Mahsa Sobhi Afshar ◽  
Mohammad Moghaddaszadeh
Keyword(s):  
Enterococcus Faecalis ◽  
Virulence Factors ◽  
Root Canal ◽  
Statistical Power ◽  
P Value ◽  
Human Teeth ◽  
Root Canals ◽  
Gutta Percha ◽  
Post Hoc

Objectives: The aim of this study was to determine the effect of MTAD on the expression of virulence factors of Enterococcus faecalis (E.faecalis) considering the role of Gutta-percha/AH26 or Resilon/RealSeal SE as root canal obturating materials. Materials and Methods: One-hundred and forty-four single-rooted human teeth were instrumented to a standardized apical size. Root canals were infected by E.faecalis (ATCC 29212). Ninety teeth were irrigated with MTAD and randomly divided into three groups. In two groups, root canals were obturated by either Gutta-percha/AH26 or Resilon/RealSeal SE. Root canals were kept unobturated in the third group. The remaining 54 teeth received no final irrigation. All groups were then subdivided into three timepoint subgroups in which dentin powder was obtained from each sample to determine the expression of specific virulence factors of E.faecalis (efa, esp, gel, fsr) using real-time reverse transcription polymerase chain reaction (RT-PCR). Statistical analysis was performed by one-way analysis of variance (ANOVA) and Tukey’s post-hoc test. The statistical power was set at P-value ≤0.05. Results: MTAD was effective against the expression of most of the tested virulence factors, and Gutta-percha/AH26 increased the antibacterial efficacy of MTAD. Conclusions: MTAD could inhibit the expression of some known virulence factors of E.faecalis at the majority of tested timepoints. This may partly explain some of the mechanisms of antimicrobial efficacy of MTAD against this resistant microorganism which is known as one of the main causes of failure of root canal treatment.

scholarly journals Role of (p)ppGpp in Biofilm Formation by Enterococcus faecalis

2011 ◽  
Vol 78 (5) ◽  
pp. 1627-1630 ◽  
Author(s):  
Luis E. Chávez de Paz ◽  
José A. Lemos ◽  
Claes Wickström ◽  
Christine M. Sedgley
Keyword(s):  
Cell Viability ◽  
Enterococcus Faecalis ◽  
Proteolytic Activity ◽  
Biofilm Formation ◽  
Extended Period ◽  
Content Type ◽  
Diminished Capacity ◽  
Growth Profiles

ABSTRACTEnterococcus faecalisstrain OG1RF and its (p)ppGpp-deficient ΔrelA, ΔrelQ, and ΔrelAΔrelQmutants were grown in biofilms and evaluated for growth profiles, biofilm morphology, cell viability, and proteolytic activity.E. faecalislacking (p)ppGpp had a diminished capacity to sustain biofilm formation over an extended period of time and expressed abundant proteolytic activity.

scholarly journals CRISPR-cas system in the acquisition of virulence genes in dental-root canal and hospital-acquired isolates of Enterococcus faecalis

2019 ◽  
Author(s):  
Pourya Gholizadeh ◽  
Mohammad Aghazadeh ◽  
Reza Ghotaslou ◽  
Mohammad Ahangarzadeh Rezaee ◽  
Tahereh Pirzadeh ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Biofilm Formation ◽  
Virulence Genes ◽  
Virulence Determinants ◽  
Root Canals ◽  
Causative Agents ◽  
Life Threatening ◽  
Selective Forces ◽  
Hospital Acquired

Abstract Introduction Enterococcus faecalis is one of the important causative agents of nosocomial and life-threatening infections in human. Several studies have demonstrated that the presence of CRISPR- cas is associated with antibiotic susceptibility and lack of virulence traits. In this study, we aimed to assess the phenotypic and genotypic virulence determinants in relation to CRISPR elements from the dental-root canals and hospital-acquired isolates of E. faecalis .Methods and materials Eighty-eight hospital-acquired and 73 dental-root canal isolates of E. faecalis were assessed in this study. Phenotypic screening of the isolates included biofilm formation, and gelatinase and hemolysis activities. Genotypical screening using PCR was further used to evaluate the presence of CRISPR elements and different virulence-associated genes such as efaA , esp , cylA , hyl , gelE , ace , ebpR , and asa1 .Results Biofilm formation, and gelatinase and hemolysis activity were detected in 93.8%, 29.2% and 19.2% of the isolates, respectively. The most prevalent virulence-associated gene was ace , which was followed by efaA , whereas cylA was the least identified. The presence of CRISPR1- cas , orphan CRISPR2 and CRISPR3- cas was determined in 13%, 55.3% and 17.4% of the isolates, respectively. CRISPR elements were significantly more prevalent in the dental-root canals isolates. An inverse significant correlation was found between CRISPR- cas loci, esp and gelE , while direct correlations were observed in the case of cylA , hyl , gelE (among CRISPR-loci 1 and 3), asa1 , ace , biofilm formation, and hemolysis activity.Conclusion Findings, therefore, indicate that CRISPR- cas might prevent the acquisition of some respective pathogenicity factors in some isolates, though not all; so selective forces could not influence pathogenic traits.

scholarly journals Pathogenicity Biofilm formation of Enterococcus faecalis

2020 ◽  
Vol 5 (1) ◽  
pp. 47-50
Author(s):  
Nur Asmah
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Biofilm Formation ◽  
Laboratory Diagnosis ◽  
Toxin Production ◽  
Limited Information ◽  
Periapical Lesions ◽  
Alkaline Conditions ◽  
Autolytic Activity ◽  
Acid Reaction

Biofilm formation is closely related to the pathogenicity of E. faecalis in persistent root canal infections. Limited information is available about the ability and characteristics of E. faecalis biofilm-forming in the persistent pathogenicity of root canal infections. Based on these problems, the purpose of this paper is to improve the molecular understanding of E. faecalis on virulence factors associated with biofilm formation against persistent root canal infections to support laboratory diagnosis and therapy of oral E. faecalis. In conclusion, the release of cytokines triggers the dlt gene of LTA to improving: homeostasis, autolytic activity, and bacterial envelope properties. The role of cytolysin activated by the cylLL and cylLS genes improving the survival ability of E. Faecalis. Hyaluronidase will facilitate other bacteria to migrate from the root canal to periapical lesions resulting in the triggering of toxin production, which will increase damage and inflammation in the host. Enterococcus faecalis, through dentine matrix formation, hydrolyze collagen and induce apatite deposition in developing biofilms. Besides, these bacteria can also increase their tolerance to antimicrobials by blocking the inflammatory response's acid reaction. Alkaline conditions will neutralize the lactic acid secreted by osteoclasts to absorb hard tissue

scholarly journals Pathogenicity Biofilm formation of Enterococcus faecalis

2020 ◽  
Vol 5 (1) ◽  
pp. 47-50
Author(s):  
Nur Asmah
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Biofilm Formation ◽  
Laboratory Diagnosis ◽  
Toxin Production ◽  
Limited Information ◽  
Periapical Lesions ◽  
Alkaline Conditions ◽  
Autolytic Activity ◽  
Acid Reaction

Biofilm formation is closely related to the pathogenicity of E. faecalis in persistent root canal infections. Limited information is available about the ability and characteristics of E. faecalis biofilm-forming in the persistent pathogenicity of root canal infections. Based on these problems, the purpose of this paper is to improve the molecular understanding of E. faecalis on virulence factors associated with biofilm formation against persistent root canal infections to support laboratory diagnosis and therapy of oral E. faecalis. In conclusion, the release of cytokines triggers the dlt gene of LTA to improving: homeostasis, autolytic activity, and bacterial envelope properties. The role of cytolysin activated by the cylLL and cylLS genes improving the survival ability of E. Faecalis. Hyaluronidase will facilitate other bacteria to migrate from the root canal to periapical lesions resulting in the triggering of toxin production, which will increase damage and inflammation in the host. Enterococcus faecalis, through dentine matrix formation, hydrolyze collagen and induce apatite deposition in developing biofilms. Besides, these bacteria can also increase their tolerance to antimicrobials by blocking the inflammatory response's acid reaction. Alkaline conditions will neutralize the lactic acid secreted by osteoclasts to absorb hard tissue

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