scholarly journals Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating Materials

2019 ◽  
Author(s):  
Behnam Bolhari ◽  
Abbas Bahador ◽  
Mehrfam Khoshkhounejad ◽  
Mahsa Sobhi Afshar ◽  
Mohammad Moghaddaszadeh
Keyword(s):  
Enterococcus Faecalis ◽  
Virulence Factors ◽  
Root Canal ◽  
Statistical Power ◽  
P Value ◽  
Human Teeth ◽  
Root Canals ◽  
Gutta Percha ◽  
Post Hoc

Objectives: The aim of this study was to determine the effect of MTAD on the expression of virulence factors of Enterococcus faecalis (E.faecalis) considering the role of Gutta-percha/AH26 or Resilon/RealSeal SE as root canal obturating materials. Materials and Methods: One-hundred and forty-four single-rooted human teeth were instrumented to a standardized apical size. Root canals were infected by E.faecalis (ATCC 29212). Ninety teeth were irrigated with MTAD and randomly divided into three groups. In two groups, root canals were obturated by either Gutta-percha/AH26 or Resilon/RealSeal SE. Root canals were kept unobturated in the third group. The remaining 54 teeth received no final irrigation. All groups were then subdivided into three timepoint subgroups in which dentin powder was obtained from each sample to determine the expression of specific virulence factors of E.faecalis (efa, esp, gel, fsr) using real-time reverse transcription polymerase chain reaction (RT-PCR). Statistical analysis was performed by one-way analysis of variance (ANOVA) and Tukey’s post-hoc test. The statistical power was set at P-value ≤0.05. Results: MTAD was effective against the expression of most of the tested virulence factors, and Gutta-percha/AH26 increased the antibacterial efficacy of MTAD. Conclusions: MTAD could inhibit the expression of some known virulence factors of E.faecalis at the majority of tested timepoints. This may partly explain some of the mechanisms of antimicrobial efficacy of MTAD against this resistant microorganism which is known as one of the main causes of failure of root canal treatment.

scholarly journals Influence of calcium hydroxide points on the quality of intracanal dressing filling

2006 ◽  
Vol 14 (3) ◽  
pp. 219-223 ◽  
Author(s):  
Nicole de Mello Rahde ◽  
José Antonio Poli Figueiredo ◽  
Elias Pandonor Motcy Oliveira
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal Filling ◽  
Human Teeth ◽  
Significance Level ◽  
Root Canals ◽  
Gutta Percha ◽  
Group 2 ◽  
Post Hoc

PURPOSE: The aim of this study was to evaluate, in vitro, the quality of calcium hydroxide [Ca(OH)2] paste filling (Ultracal, Ultradent) associated or not with Ca(OH)2-containing gutta-percha points (Calcium Hydroxide Plus PointsTM, Roeko) in curved root canals. MATERIAL AND METHODS: One hundred and twenty roots of extracted human teeth, randomly divided into three curvature ranges (mild - 0 to 14º; moderate - 15º to 29º; severe - >30º) were used. After chemomechanical preparation, the roots were assigned to 4 groups (n=30), according to the technique of intracanal dressing placement: group 1 - Ca(OH)2 paste was applied with a lentulo spiral; group 2 - Ca(OH)2 paste was applied with a lentulo spiral and a Ca(OH)2 point was inserted into the canal; group 3 - Ca(OH)2 paste was applied with a NavitipTM tip (supplied with Ultracal system); group 4 - Ca(OH)2 paste was applied with a NavitipTM tip and a Ca(OH)2 point was inserted into the canal. The roots were cleared and the quality of apical third filling was assessed by a calibrated experienced examiner. The specimens were examined under stereomicroscopy and scored 1 to 4 (i.e., from inadequate to complete root canal filling). The results were analyzed statistically by ANOVA and Duncan's post hoc test at 5% significance level. RESULTS: There were no statistically significant differences (p>0.05) among the curvature degrees in groups 1, 3 and 4. Severely curved roots in group 2 presented bordering significance (p=0.05). The groups that associated the use of Ca(OH)2 paste and points (2 and 4) showed better apical filling than the other groups, but this difference was statistically significant (p<0.001) only for roots with severe curvature. CONCLUSION: According to the results of this study, the curvature degree did not influence the quality of filling. The techniques that used Ca(OH)2-containing gutta-percha points yielded better filling of the apical third in roots with severe curvature.

scholarly journals Effect of a sonic device on bonding of fiberglass posts to root canals

2018 ◽  
Vol 17 ◽  
pp. e18924
Author(s):  
Marina de Almeida Salim ◽  
Thais Mageste Duque ◽  
Heloisa Carla Dell Santo Gusman ◽  
Carolina Oliveira de Lima ◽  
Brenda Paula Figueiredo de Almeida Gomes ◽  
...  
Keyword(s):  
Root Canal ◽  
Adhesive System ◽  
Resin Cement ◽  
Root Canal Filling ◽  
Root Canals ◽  
Gutta Percha ◽  
Ah Plus ◽  
Root Dentin ◽  
Post Hoc ◽  
Adhesive Cement

Aim: This study evaluated the effect of a sonic device on the bonding of fiberglass posts cemented with a self-etching adhesive combined with conventional cement or a self-adhesive cement to root dentin. Methods: Forty single-rooted bovine incisors were endodontically prepared using a step-back technique. Gutta-percha and AH Plus sealer were used for root canal filling, combined with a thermoplasticization technique. After 1 week, the post space was prepared and the roots were divided into four groups according to the following factors: adhesive system/resin cement (Ambar/ AllCem Core [FGM] and RelyX U200 [3M]) and application mode (manual or sonic). The posts were cemented and the roots were cut into discs and submitted to push-out bond strength (POBS) test. The failure mode was evaluated using a stereoscope at 25x magnification. The data were analyzed statistically using ANOVA and Tukey’s post-hoc test (α = 0.05). Results: The sonically activated RelyX U200 group was superior to the other groups. RelyX U200 manually applicated showed similar results to those of sonically activated Âmbar/AllCem. These three groups outperformed the Âmbar/AllCem manually applicated group. Adhesive failure between dentin and resin cement was the most predominant pattern. Conclusion: Sonic application of self-etching adhesive and self-adhesive cement improved the POBS of fiber posts to the root canal.

scholarly journals Evaluation of the WaveOne Gold and One Shape New Generation in Reducing Enterococcus faecalis from Root Canal

2018 ◽  
Vol 29 (3) ◽  
pp. 249-253 ◽  
Author(s):  
Raquel Esmeralda Guillén ◽  
Cleber Keiti Nabeshima ◽  
Hector Caballero-Flores ◽  
Miguel Roig Cayón ◽  
Montse Mercadé ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
File Systems ◽  
Bacterial Reduction ◽  
Root Canals ◽  
Single File ◽  
Bacterial Removal ◽  
Significant Difference ◽  
Post Hoc ◽  
New Generation

Abstract The aim of this study was to evaluate the WaveOne Gold and One Shape New Generation systems regarding the bacterial removal from root canals infected with Enterococcus faecalis by comparing them to the conventional WaveOne and One Shape systems. Forty-eight distobuccal root canals of maxillary molars sterilized with ethylene oxide were infected with E. faecalis for 21 days, and then root canal initial bacterial sample was collected with paper cones and plated on M-enterococcus agar. The specimens were randomly divided into 4 groups according to the instrumentation: WaveOne Gold, One Shape New Generation, WaveOne and One Shape. After instrumentation, samples were collected with use of scraping and paper cones at immediate and 7 days after instrumentation. The bacterial reduction was calculated and then made intragroup analysis by Friedman test and intergroup analysis by Kruskal-Wallis with Dunn’s post-hoc test, all at 5% significance. All techniques significantly reduced the number of bacteria in the root canal (p<0.05). WaveOne Gold and One Shape New Generation promoted higher bacterial reduction than WaveOne and One Shape systems (p<0.05), but no significant difference was found between WaveOne Gold and One Shape New Generation or between WaveOne and One Shape (p>0.05). Novel single-file systems promote better bacterial removal than the conventional single-file systems.

scholarly journals Comparative study of coronal sealing ability using root cross sections method

2007 ◽  
Vol 54 (4) ◽  
pp. 224-230
Author(s):  
Dejan Markovic ◽  
Vesna Zivojinovic ◽  
Slavoljub Zivkovic
Keyword(s):  
Cross Sections ◽  
Root Canal ◽  
Smear Layer ◽  
Blue Dye ◽  
Human Teeth ◽  
Root Canals ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Significant Difference

Objective: An in vitro comparison of coronal microleakage of three root canal sealers was performed using the dye leakage method. Methodology: Ninety-eight single-rooted human teeth were instrumented and randomly allocated to two groups. In the first group, root canals were treated with EDTA to remove the smear layer. The roots in both groups were obturated using laterally condensed gutta-percha and Sealapex, AH-26 or Ketac-Endo as the sealer. The teeth were suspended in 1% methylene blue dye for 6 days. The teeth were then rinsed, sectioned perpendicular to the long axis and evaluated for coronal dye penetration. Results: In general, Ketac-Endo root canal sealer showed greater dye penetration than Sealapex and AH-26. A statistically significant difference in the coronal penetration of the dye was found in the group with intact smear layer. Conclusion: Highly significant differences between AH-26 and Ketac-Endo (p < 0.001) and Sealapex and Ketac Endo (p < 0.001) were found.

scholarly journals Efficacy of Reciprocating Instruments in the Removal of Bioceramic and Epoxy Resin-Based Sealers: Micro-CT Analysis

Materials ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 6670
Author(s):  
Marko Rajda ◽  
Ivana Miletić ◽  
Gorana Baršić ◽  
Silvana Jukić Krmek ◽  
Damir Šnjarić ◽  
...  
Keyword(s):  
Epoxy Resin ◽  
Root Canal ◽  
Filling Material ◽  
Micro Ct ◽  
Root Canal Filling ◽  
Human Teeth ◽  
Root Canals ◽  
Gutta Percha ◽  
Ct Analysis ◽  
Reciprocating Instruments

The objective of this study was to evaluate the efficacy of reciprocating instruments in the removal of bioceramic and epoxy resin-based sealers using micro-CT analysis. Root canals of 40 extracted human teeth were instrumented with a size R25 Reciproc instrument. Specimens were randomly divided into two groups (n = 20) according to the root canal filling material. In the first group, root canals were obturated with AH Plus sealer and Reciproc R25 gutta-percha. In the second group, a combination of bioceramic gutta-percha (TotalFill BC) and bioceramic sealer (TotalFill BC) was used. After one week, the retreatment of all specimens was performed using Reciproc instruments. To analyze the differences in the filling remnants, specimens were scanned in a micro-CT device after obturation and after the retreatment procedure. Statistical analysis was performed using the Mann–Whitney U test (p < 0.05). A combination of bioceramic sealer and bioceramic gutta-percha was more effectively removed from canals using a reciprocating instrument, with a filling remnants volume of 4.01 ± 3.13 mm3, in comparison to the combination of epoxy resin-based sealer and gutta-percha (6.96 ± 2.70 mm3) (p < 0.05). A reciprocating instrument was more effective in removing bioceramic sealers than epoxy resin-based sealers, although none of the root canal filling materials were completely removed from the root canals.

scholarly journals Effect of Super-Bond C&B and self-adhesive dual-cured resin cement on the fracture resistance of roots with vertical root fracture

2019 ◽  
Vol 13 (2) ◽  
pp. 153-157
Author(s):  
Ezgi Doğanay Yıldız ◽  
Hakan Arslan ◽  
Nilay Ayaz ◽  
Mustafa Gündoğdu ◽  
Alper Özdoğan ◽  
...  
Keyword(s):  
Root Canal ◽  
Fracture Resistance ◽  
Resin Cement ◽  
Control Group ◽  
Human Teeth ◽  
Root Fracture ◽  
Vertical Root Fracture ◽  
Root Canals ◽  
Chi Squared ◽  
Post Hoc

Background. Vertical root fracture might occur during root canal preparation, obturation, post procedures or endodontic treatment. Methods. Fifty-four single-rooted human teeth were decoronated to obtain a standardized length. The root canals were enlarged up to #50 and obturated with gutta-percha and root canal sealer. Eighteen teeth were used as a control group, and vertical root fracture was induced in the remaining teeth. The samples were randomly divided into three groups, as follows: control group (without vertical root fracture), Super-Bond C&B group (fragments were attached with Super-Bond C&B), and self-adhesive dual-cured resin cement group (fragments were attached with self-adhesive dual-cured resin cement). Each specimen was subjected to a fracture resistance test, and data were statistically analyzed using chi-squared test, one-way ANOVA and post hoc Tukey tests (P=0.05). Results. The fracture resistance values of the control and Super-Bond C&B groups were higher than those of the self-adhesive dual-cured resin cement group (P<0.05). However, there were no significant differences between the control and Super-Bond C&B groups (P>0.05). Conclusion. Within the limitations of the present study, Super-Bond C&B was beneficial in obtaining higher fracture resistance in endodontically treated roots with vertical root fracture.

scholarly journals Improving the Efficiency of Photodynamic Chemotherapy in Root Canals against Enterococcus faecalis In Vitro

Antibiotics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 543
Author(s):  
Christian Tennert ◽  
Yoana Zinovieva ◽  
Kalin Shishkov ◽  
Lamprini Karygianni ◽  
Makus Jörg Altenburger ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antimicrobial Effect ◽  
Antimicrobial Treatment ◽  
Human Teeth ◽  
Treatment Groups ◽  
Root Canals ◽  
Negative Root ◽  
Culture Negative

The aim of this study was to evaluate the effect of photoactivated chemotherapy (PACT) on Enterococcus faecalis (E. faecalis) biofilms in root canals using an 90% isopropanol (IPA)-based photosensitizer and removing excess photosensitizer before light incubation. Three hundred and seven extracted human teeth with one root canal were infected with E. faecalis for 72 h and treated in groups: IPA irrigation; PACT; PACT and final rinse with IPA; PACT with photosensitizer removal using either 0.9% NaCl solution or sterile paper points or both; PACT using IPA-based photosensitizer with and without a final rinse of IPA. Root canals were sampled using sterile paper points and dentin chips collected from the root canal walls. Additionally, SEM (Scanning Electron Microscopy) images of the specimens were taken to evaluate the root canal walls for residue bacterial contamination. In all antimicrobial treatment groups treatments E. faecalis counts were significantly reduced in the root canals. Using IPA-based photosensitizer the antimicrobial effect of PACT was significantly enhanced. Irrigation with IPA alone or after PACT significantly increased the antimicrobial effect compared to PACT alone. The collected dentin chips revealed the highest amount of culture negative root canals (10%) after PACT using IPA-based photosensitizer. In the other groups, the culture negative samples ranged from only 0–2 specimens of 30 specimens. REM images show remaining E. faecalis cells on the root canal wall and inside dentin tubules. Using IPA-based photosensitizer significantly enhanced the antimicrobial effect of PACT against E. faecalis in the root canals.

scholarly journals Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals

2016 ◽  
Vol 17 (2) ◽  
pp. 114-118 ◽  
Author(s):  
Marcia C Valera ◽  
Sarah AC Oliveira ◽  
Lilian E Maekawa ◽  
Flávia GR Cardoso ◽  
Adriana Chung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Zingiber Officinale ◽  
Human Teeth ◽  
Root Canals

ABSTRACT Aim The purpose of this in vitro study was to evaluate the antimicrobial activity of 2% chlorhexidine gel (CHX) as auxiliary chemical substance and intracanal medications on Candida albicans, Candida albicans, Enterococcus faecalis, Escherichia coli, and their endotoxins in the root canals. Materials and methods The study was conducted on 48 single-rooted human teeth divided into four groups (n = 12), according to intracanal medications used: (1) Calcium hydroxide + apyrogenic saline solution (Ca(OH)2 + SS), (2) 20% ginger glycolic extract (GEN), (3) calcium hydroxide + 20% ginger glycolic extract (Ca(OH)2 + GEN), (4) apyrogenic SS (control). Collections were made from the root canal content before preparation (baseline–S1), immediately after instrumentation (S2), 7 days after instrumentation (S3), after 14 days the action of intracanal medication (S4), and 7 days after removal of the intracanal medication (S5). The antimicrobial activity and endotoxin content were analyzed for all collections. The results were statistically analyzed by the Kruskal–Wallis and Dunn tests at a significance level of 5%. Results After instrumentation with CHX, there was complete elimination of E. coli and C. albicans, except for E. faecalis, which was significantly reduced and then completely eliminated after intracanal medication. There was significant reduction of endotoxin after instrumentation. Comparison of collection after instrumentation and intracanal medication revealed reduction of endotoxins in all groups; this reduction was greater in group Ca(OH)2 followed by the group GEN. Conclusion It was concluded that the instrumentation using CHX and intracanal medication used were able to eliminate the microorganisms from the root canal; the endotoxins were reduced, yet not completely eliminated. Clinical significance This study is important and relevant for searching alternatives during endodontic therapy, since it aims to study the effect of Zingiber officinale on microorganisms and endotoxins present in root canals. How to cite this article Valera MC, Oliveira SAC, Maekawa LE, Cardoso FGR, Chung A, Silva SFP, Carvalho CAT. Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals. J Contemp Dent Pract 2016; 17(2):114-118.

scholarly journals Comparative study of the sealing ability of a polydimethylsiloxane-based root canal sealer

2005 ◽  
Vol 16 (2) ◽  
pp. 145-148 ◽  
Author(s):  
Nickolaos Economides ◽  
Beltes Panagiotis ◽  
Ioannis Kolokouris ◽  
Christos Gogos ◽  
Ioannis Kokorikos
Keyword(s):  
Root Canal ◽  
Fluid Transport ◽  
Transport Model ◽  
Smear Layer ◽  
The Other ◽  
Human Teeth ◽  
Root Canals ◽  
Gutta Percha ◽  
Sealing Ability ◽  
Root Canal Sealers

The purpose of this study was to compare the sealing ability of three root canal sealers, RSA (polydimethylsiloxane sealer), Endion (glass-ionomer sealer) and Topseal (epoxy resin sealer), with and without smear layer removal. Ninety extracted human teeth were selected and assigned to 6 groups (n=15), according to the following protocols: in 3 groups, the smear layer was left intact and the root canals were obturated with gutta-percha points and RSA, Endion and Topseal, respectively; in the other 3 groups, the smear layer was removed and the root canals were obturated in the same way as described above. Microleakage was measured at 7 days, 1 month and 2 months, using the fluid transport model. The results were expressed in µL/24 h. Data were analyzed statistically by Kruskall-Wallis and Mann-Whitney non-parametric tests. The results showed that the Topseal group without smear layer leaked significantly less (p<0.05) than the RSA group without smear layer, at all experimental periods. No statistically significant differences (p>0.05) were found among the other groups, at the established evaluation intervals. The findings of this study showed that the polydimethylsiloxane sealer (RSA) did not yield better sealing ability than the other sealers, either in the presence or in the absence of smear layer. Topseal had the least microleakage from the root canal sealers evaluated.

scholarly journals The effect of different root canal medicaments on the elimination of Enterococcus faecalis ex vivo

2013 ◽  
Vol 07 (04) ◽  
pp. 442-448 ◽  
Author(s):  
Till Dammaschke ◽  
Nina Jung ◽  
Inga Harks ◽  
Edgar Schafer
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Ex Vivo ◽  
Agar Plate ◽  
Antimicrobial Effect ◽  
Human Teeth ◽  
Sterile Saline ◽  
Root Canals ◽  
Root Segments ◽  
Positive Controls

ABSTRACT Objective: The aim of this study was to evaluate the antimicrobial effect of chlorhexidine gel (CHX-G) 2%, chlorhexidine powder (CHX-P) 1%, povidone-iodine (PVP-I), polyhexanide and camphorated-and-mentholated chlorophenol (ChKM) ex vivo. Materials and Methods: For every medicament group 10 root segments (15 mm long) of extracted human teeth were prepared to ISO-size 45 and sterilized (n = 50). The root segments were then inoculated with Enterococcus faecalis and aerobically incubated at 37°C. After 1 week, ten root canals were filled with one of the medicaments, respectively and aerobically incubated at 37°C for another week. Ten teeth served as positive controls and were filled with sterile saline solution. After 7 days, the medicaments were inactivated and all root canals were instrumented to ISO-size 50. The obtained dentin samples were dispersed in Ringer solution followed by the preparation of serial dilutions. 10 ΅l per sample were applied to an agar plate and incubated at 37°C for 48 h. The colony forming units were counted and the reduction factors (RFs) were calculated and statistically analyzed. Results: Compared with the positive controls all medicaments exhibited an antibacterial effect against E. faecalis. The RFs for CHX-G, CHX-P and ChKM were significantly higher compared to PVP-I and polyhexanide (P < 0.05). In contrast to PVP-I and polyhexanide, CHX-G, CHX-P and ChKM were able to eliminate E. faecalis from all dentin samples. Conclusions: Within the limitations of this ex vivo investigation, 2% CHX-G and CHX-P were as effective as ChKM against E. faecalis. Thus, when choosing a root canal medicament the better biocompatibility of CHX compared with ChKM should be taken in consideration.

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