Histological and immunohistochemical study on the possible protective effect of curcumin on intestinal ischemia/reperfusion-induced lung injury in albino rats

Intestinal ischemia/reperfusion (I/R) is a clinical emergency, which often causes lung injury with high morbidity and mortality. Although dexmedetomidine has been identified to have a protective effect on lung injury caused by intestinal I/R, its specific mechanism is still elucidated. In recent years, the cannabinoid (CB2) receptor pathway has been found to be involved in I/R injury of some organs. In the current study, we investigated whether the CB2 receptor pathway contributes to the protective effect of dexmedetomidine on the intestinal I/R-induced lung injury in rats. Dexmedetomidine treatment upregulated the expression of CB2 receptor and suppressed the I/R-induced increases in lung injury scores, inflammatory cell infiltration, lung wet/dry ratio, MPO activity, MDA level, inflammatory cytokines, and caspase-3 expression while augmenting SOD activity and Bcl-2 expression, indicating attenuation of lung injury. Dexmedetomidine treatment also increased the expression of Akt. The protective effects of dexmedetomidine treatment were reversed by the CB2 receptor antagonist AM630 or the PI3K inhibitor wortmannin. And the CB2 receptor antagonist AM630 also downregulated the expression of Akt. Thus, our findings suggest that treatment with dexmedetomidine provides a protective role against lung injury caused by intestinal I/R in rats, possibly due to the upregulation of the CB2 receptor, followed by the activation of the PI3K/Akt pathway.

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Pretreatment with pentoxifylline attenuates lung injury induced by intestinal ischemia/reperfusion in rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502011000600006 ◽

2011 ◽

Vol 26 (6) ◽

pp. 438-444 ◽

Cited By ~ 9

Author(s):

Carlos Eduardo Marqui ◽

Helga Cristina Almeida Silva ◽

David Ferez ◽

Sâmia Santos Cavassani ◽

Juliana Britto Moraes ◽

...

Keyword(s):

Lung Injury ◽

Protective Effect ◽

Superior Mesenteric Artery ◽

Mesenteric Artery ◽

Intestinal Ischemia ◽

Ischemia Reperfusion ◽

Weight Ratio ◽

Arterial Occlusion ◽

Myeloperoxidase Activity ◽

Intestinal Ischemia Reperfusion

PURPOSE: To investigate the protective effect of pentoxifylline against the lung injury observed after intestinal ischemia (I) followed by a period of reperfusion (R). METHODS: Twenty-eight male Wistar rats were equally divided into 4 experimental groups and operated under ketamine-xylazine anesthesia. (1) Sham: falsely-operated animals; (2) SS+IR: intestinal ischemia was accomplished by clipping the superior mesenteric artery during 60 minutes, with an administration of a standard volume of saline solution (SS) 5 min before the end of the ischemia period; the clip was then releases or a 120-min period of reperfusion; (3) I+PTX+R: ischemia as above, PTX was administered (25 mg/kg) and the gut reperfused as above; (4) PTX+I+PTX+R: Five minutes before arterial occlusion PTX was administered; the superior mesenteric artery was then clipped for 60 minutes. After 55-min ischemia, an additional dosis of PTX was administered; the clip was removed for reperfusion as above. At the 60th min of reperfusion a third dosis of PTX was administered. RESULTS: PTX markedly attenuated lung injury as manifested by significant decreases (all P<0.001 as compared with the SS+IR group) of pulmonary wet/dry tissue weight ratio, total protein content, myeloperoxidase activity and tumor necrosis factor-alpha. Moreover, it was apparent that in the group PTX+I+PTX+R the improvements have been even more significant. CONCLUSION: PTX exerted a protective effect on the lung from the injuries caused by intestinal ischemia/reperfusion.

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Protective Effect of Probiotic Potentiate with Thiopental in Intestinal Ischemia-Reperfusion Induced Lung Injury

International Journal of Pharmacology ◽

10.3923/ijp.2019.872.879 ◽

2019 ◽

Vol 15 (7) ◽

pp. 872-879

Author(s):

Wenxia Jia ◽

Zhijia Guo ◽

Xiang Yu ◽

Jieping Lv ◽

Baozhong Yang ◽

...

Keyword(s):

Lung Injury ◽

Protective Effect ◽

Intestinal Ischemia ◽

Ischemia Reperfusion ◽

Intestinal Ischemia Reperfusion

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Ginsenoside Rb1 Treatment Attenuates Pulmonary Inflammatory Cytokine Release and Tissue Injury following Intestinal Ischemia Reperfusion Injury in Mice

Oxidative Medicine and Cellular Longevity ◽

10.1155/2015/843721 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 22

Author(s):

Ying Jiang ◽

Zhen Zhou ◽

Qing-tao Meng ◽

Qian Sun ◽

Wating Su ◽

...

Keyword(s):

Lung Injury ◽

Signaling Pathway ◽

Intestinal Ischemia ◽

Ischemia Reperfusion ◽

Critical Role ◽

Weight Ratio ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Ginsenoside Rb1 ◽

Intestinal Ischemia Reperfusion

Objective. Intestinal ischemia reperfusion (II/R) injury plays a critical role in remote organ dysfunction, such as lung injury, which is associated with nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. In the present study, we tested whether ginsenoside Rb1 attenuated II/R induced lung injury by Nrf2/HO-1 pathway.Methods. II/R injury was induced in male C57BL/6J mice by 45 min of superior mesenteric artery (SMA) occlusion followed by 2 hours of reperfusion. Ginsenoside Rb1 was administrated prior to reperfusion with or without ATRA (all-transretinoic acid, the inhibitor of Nrf2/ARE signaling pathway) administration before II/R.Results. II/R induced lung histological injury, which is accompanied with increased levels of malondialdehyde (MDA), interleukin- (IL-) 6, and tumor necrosis factor- (TNF-)αbut decreased levels of superoxide dismutase (SOD) and IL-10 in the lung tissues. Ginsenoside Rb1 reduced lung histological injury and the levels of TNF-αand MDA, as well as wet/dry weight ratio. Interestingly, the increased Nrf2 and HO-1 expression induced by II/R in the lung tissues was promoted by ginsenoside Rb1 treatment. All these changes could be inhibited or prevented by ATRA.Conclusion. Ginsenoside Rb1 is capable of ameliorating II/R induced lung injuries by activating Nrf2/HO-1 pathway.

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