Modulation of In Vitro Microenvironment Facilitates Synovium-Derived Stem Cell-Based Nucleus Pulposus Tissue Regeneration

Spine ◽  
2012 ◽  
Vol 37 (18) ◽  
pp. 1538-1547 ◽  
Author(s):  
Ming Pei ◽  
Mark Shoukry ◽  
Jingting Li ◽  
Scott D. Daffner ◽  
John C. France ◽  
...  
Keyword(s):  
Stem Cell ◽  
Nucleus Pulposus ◽  
Tissue Regeneration ◽  
Nucleus Pulposus Tissue

Amniotic Stem Cell-Conditioned Media for the Treatment of Nerve and Muscle Pathology: A Systematic Review

2021 ◽  
Author(s):  
Chukwuweike Gwam ◽  
Ahmed Emara ◽  
Nequesha Mohamed ◽  
Noor Chughtai ◽  
Johannes Plate ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Cell Damage ◽  
Conditioned Media ◽  
Nerve Tissue ◽  
Muscle Pathology ◽  
Loss Of Function ◽  
Cell Based Therapy

Muscle and nerve tissue damage can elicit a significant loss of function and poses as a burden for patients and healthcare providers. Even for tissues, such as the peripheral nerve and skeletal muscle, that harbor significant regenerative capacity, innate regenerative processes often lead to less than optimal recovery and residual loss of function. The reasons for poor regeneration include significant cell damage secondary to oxidative stress, poor recruitment of resident stem cells, and an unfavorable microenvironment for tissue regeneration. Stem cell-based therapy was once thought as a potential therapy in tissue regeneration, due to its self-renewal and multipotent capabilities. Early advocates for cellular-based therapy pointed to the pluripotent nature of stem cells, thus eluding to its ability to differentiate into resident cells as the source of its regenerative capability. However, increasing evidence has revealed a lack of engraftment and differentiation of stem cells, thereby pointing to stem cell paracrine activity as being responsible for its regenerative potential. Stem cell-conditioned media houses biomolecular factors that portray significant regenerative potential. Amniotic-derived stem cell-conditioned media (AFS-CM) has been of particular interest because of its ease of allocation and in vitro culture. The purpose of this review is to report the results of studies that assess the role of AFS-CM for nerve and muscle conditions. In this review, we will cover the effects of AFS-CM on cellular pathways, genes, and protein expression for different nerve and muscle cell types.

scholarly journals Inorganic polyphosphates enhances nucleus pulposus tissue formation in vitro

2016 ◽  
Vol 35 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Rahul Gawri ◽  
Toshikazu Shiba ◽  
Robert Pilliar ◽  
Rita Kandel
Keyword(s):  
Nucleus Pulposus ◽  
Tissue Formation ◽  
Inorganic Polyphosphates ◽  
Nucleus Pulposus Tissue

Effect of circumferential constraint on nucleus pulposus tissue in vitro

2010 ◽  
Vol 10 (2) ◽  
pp. 174-183 ◽  
Author(s):  
Darla J. Hamilton ◽  
Robert M. Pilliar ◽  
Stephen Waldman ◽  
Rita A. Kandel
Keyword(s):  
Nucleus Pulposus ◽  
Nucleus Pulposus Tissue

scholarly journals In vitro nucleus pulposus tissue model with physicochemical stresses

JOR Spine ◽  
2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Yoshiki Takeoka ◽  
James D. Kang ◽  
Shuichi Mizuno
Keyword(s):  
Nucleus Pulposus ◽  
Tissue Model ◽  
Nucleus Pulposus Tissue

scholarly journals Human Heart Explant-Derived Extracellular Vesicles: Characterization and Effects on the In Vitro Recellularization of Decellularized Heart Valves

2019 ◽  
Vol 20 (6) ◽  
pp. 1279 ◽  
Author(s):  
Amanda Leitolis ◽  
Paula Suss ◽  
João Roderjan ◽  
Addeli Angulski ◽  
Francisco da Costa ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Extracellular Vesicles ◽  
Tissue Regeneration ◽  
Human Heart ◽  
Heart Valves ◽  
Enrichment Analysis ◽  
Cell Types

Extracellular vesicles (EVs) are particles released from different cell types and represent key components of paracrine secretion. Accumulating evidence supports the beneficial effects of EVs for tissue regeneration. In this study, discarded human heart tissues were used to isolate human heart-derived extracellular vesicles (hH-EVs). We used nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) to physically characterize hH-EVs and mass spectrometry (MS) to profile the protein content in these particles. The MS analysis identified a total of 1248 proteins. Gene ontology (GO) enrichment analysis in hH-EVs revealed the proteins involved in processes, such as the regulation of cell death and response to wounding. The potential of hH-EVs to induce proliferation, adhesion, angiogenesis and wound healing was investigated in vitro. Our findings demonstrate that hH-EVs have the potential to induce proliferation and angiogenesis in endothelial cells, improve wound healing and reduce mesenchymal stem-cell adhesion. Last, we showed that hH-EVs were able to significantly promote mesenchymal stem-cell recellularization of decellularized porcine heart valve leaflets. Altogether our data confirmed that hH-EVs modulate cellular processes, shedding light on the potential of these particles for tissue regeneration and for scaffold recellularization.

TNF-α Induces MMP2 Gelatinase Activity and MT1-MMP Expression in an In Vitro Model of Nucleus Pulposus Tissue Degeneration

Spine ◽  
2008 ◽  
Vol 33 (4) ◽  
pp. 356-365 ◽  
Author(s):  
Cheryle A. Séguin ◽  
Robert M. Pilliar ◽  
Joseph A. Madri ◽  
Rita A. Kandel
Keyword(s):  
Nucleus Pulposus ◽  
In Vitro Model ◽  
Gelatinase Activity ◽  
Nucleus Pulposus Tissue ◽  
Tissue Degeneration ◽  
Tnf Α ◽  
Vitro Model

In Vitro Tissue Regeneration by Human Degenerated Nucleus Pulposus Cells in Hyperosmotic Culture Medium

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376656-s-0034-1376656
Author(s):  
A. Krouwels ◽  
J. Popov ◽  
S. Plomp ◽  
W. Dhert ◽  
C. Oner ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Tissue Regeneration ◽  
Culture Medium ◽  
Nucleus Pulposus Cells

scholarly journals BMP2 and TGF-β Cooperate Differently during Synovial-Derived Stem-Cell Chondrogenesis in a Dexamethasone-Dependent Manner

Cells ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 636 ◽  
Author(s):  
Nikolas Kovermann ◽  
Valentina Basoli ◽  
Elena Della Bella ◽  
Mauro Alini ◽  
Christoph Lischer ◽  
...  
Keyword(s):  
Stem Cell ◽  
Tissue Regeneration ◽  
Chondrogenic Differentiation ◽  
Cartilage Tissue ◽  
Dependent Manner ◽  
Free Medium ◽  
Epigenetic Memory ◽  
Tissue Of Origin ◽  
Tgf Β1

Recent studies highlighting mesenchymal stem cell (MSC) epigenetic memory suggest that a different differentiation medium may be required depending on the tissue of origin. As synovial-derived stem cells (SDSCs) attract interest we aimed to investigate the influence of TGF-β1, BMP-2 and dexamethasone on SDSC chondrogenesis in vitro. We demonstrate that dexamethasone-free medium led to enhanced chondrogenic differentiation at both the mRNA and matrix level. The greatest COL2A1/COL10A1 ratio was detected in cells exposed to a combination medium containing 10 ng/mL BMP-2 and 1 ng/mL TGF-β1 in the absence of dexamethasone, and this was reflected in the total amount of glycosaminoglycans produced. In summary, dexamethasone-free medium containing BMP-2 and TGF-β1 may be the most suitable when using SDSCs for cartilage tissue regeneration.

scholarly journals An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue

2009 ◽  
Vol 11 (1) ◽  
pp. R20 ◽  
Author(s):  
Christine L Le Maitre ◽  
Pauline Baird ◽  
Anthony J Freemont ◽  
Judith A Hoyland
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
In Vitro Study ◽  
Vitro Study ◽  
Nucleus Pulposus Tissue
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