Gene expression changes during the development of estrogen-independent and antiestrogen-resistant growth in breast cancer cell culture models

2009 ◽  
Vol 20 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Pasi T. Pennanen ◽  
Nanna S. Sarvilinna ◽  
Timo J. Ylikomi
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Culture ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Culture Models ◽  
Breast Cancer Cell Culture ◽  
Cell Culture Models

scholarly journals Assessing Advantages and Drawbacks of Rapidly Generated Ultra-Large 3D Breast Cancer Spheroids: Studies with Chemotherapeutics and Nanoparticles

2020 ◽  
Vol 21 (12) ◽  
pp. 4413 ◽  
Author(s):  
Austin R. Holub ◽  
Anderson Huo ◽  
Kavil Patel ◽  
Vishal Thakore ◽  
Pranav Chhibber ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Culture Models ◽  
Cell Culture Models ◽  
Mcf 7

Traditionally, two-dimensional (2D) monolayer cell culture models have been used to study in vitro conditions for their ease of use, simplicity and low cost. However, recently, three-dimensional (3D) cell culture models have been heavily investigated as they provide better physiological relevance for studying various disease behaviors, cellular activity and pharmaceutical interactions. Typically, small-sized tumor spheroid models (100–500 μm) are used to study various biological and physicochemical activities. Larger, millimetric spheroid models are becoming more desirable for simulating native tumor microenvironments (TMEs). Here, we assess the use of ultra-large spheroid models (~2000 μm) generated from scaffolds made from a nozzle-free, ultra-high resolution printer; these models are explored for assessing chemotherapeutic responses with molecular doxorubicin (DOX) and two analogues of DoxilⓇ (Dox-NPⓇ, DoxovesTM) on MDA-MB-231 and MCF-7 breast cancer cell lines. To provide a comparative baseline, small spheroid models (~500 μm) were developed using a self-aggregation method of MCF-7 breast cancer cell lines, and underwent similar drug treatments. Analysis of both large and small MCF-7 spheroids revealed that Dox-NP tends to have the highest level of inhibition, followed by molecular doxorubicin and then Doxoves. The experimental advantages and drawbacks of using these types of ultra-large spheroids for cancer research are discussed.

Effect of Graphene Oxide Incorporation into Electrospun Cellulose Acetate Scaffolds on Breast Cancer Cell Culture

2019 ◽  
Vol 20 (8) ◽  
pp. 1577-1585 ◽  
Author(s):  
Yizao Wan ◽  
Zhonghong Lin ◽  
Deqiang Gan ◽  
Teng Cui ◽  
Meirong Wan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Graphene Oxide ◽  
Cellulose Acetate ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Culture

scholarly journals Electrospinning PCL Scaffolds Manufacture for Three-Dimensional Breast Cancer Cell Culture

Polymers ◽  
2017 ◽  
Vol 9 (12) ◽  
pp. 328 ◽  
Author(s):  
Marc Rabionet ◽  
Marc Yeste ◽  
Teresa Puig ◽  
Joaquim Ciurana
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Three Dimensional ◽  
Breast Cancer Cell Culture

scholarly journals PEG-fibrinogen hydrogels for three-dimensional breast cancer cell culture

2016 ◽  
Vol 105 (1) ◽  
pp. 236-252 ◽  
Author(s):  
Shantanu Pradhan ◽  
Iman Hassani ◽  
Wen J. Seeto ◽  
Elizabeth A. Lipke
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Three Dimensional ◽  
Breast Cancer Cell Culture

scholarly journals A dataset on the effect of exercise-conditioned human sera in three-dimensional breast cancer cell culture

Data in Brief ◽  
2019 ◽  
Vol 27 ◽  
pp. 104704 ◽  
Author(s):  
Mauro De Santi ◽  
Giulia Baldelli ◽  
Francesco Lucertini ◽  
Valentina Natalucci ◽  
Giorgio Brandi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Three Dimensional ◽  
Human Sera ◽  
Breast Cancer Cell Culture
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