Identification of potential druggable targets of cell cycle with small-molecule inhibitors in oral squamous cell carcinoma

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Xiaoyi Zhou ◽  
Wenke Jin ◽  
Yanmei Chen ◽  
Lingjuan Zhu ◽  
Anchun Mo ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Small Molecule ◽  
Squamous Cell ◽  
Small Molecule Inhibitors ◽  
Druggable Targets

Cyclosporine A Suppresses the Malignant Progression of Oral Squamous Cell Carcinoma in vitro

2019 ◽  
Vol 19 (2) ◽  
pp. 248-255 ◽  
Author(s):  
Ling Gao ◽  
Jianwei Dong ◽  
Nanyang Zhang ◽  
Zhanxian Le ◽  
Wenhao Ren ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cyclosporine A ◽  
Migration And Invasion ◽  
Signal Molecules ◽  
Cox 2

Background:The Oral Squamous Cell Carcinoma (OSCC) is one of the most frequent cancer types. Failure of treatment of OSCC is potentially lethal because of local recurrence, regional lymph node metastasis, and distant metastasis. Chemotherapy plays a vital role through suppression of tumorigenesis. Cyclosporine A (CsA), an immunosuppressant drug, has been efficiently used in allograft organ transplant recipients to prevent rejection, and also has been used in a subset of patients with autoimmunity related disorders. The present study aims to investigate novel and effective chemotherapeutic drugs to overcome drug-resistance in the treatment of OSCC.Methods:Cells were incubated in the standard way. Cell viability was assayed using the MTT assay. Cell proliferation was determined using colony formation assay. The cell cycle assay was performed using flow cytometry. Apoptosis was assessed using fluorescence-activated cell sorting after stained by the Annexin V-fluorescein isothiocyanate (FITC). Cell migration and invasion were analyzed using wound healing assay and tranwell. The effect of COX-2, c-Myc, MMP-9, MMP-2, and NFATc1 protein expression was determined using Western blot analysis while NFATc1 mRNA expression was determined by RT-PCR.Results:In vitro studies indicated that CsA inhibited partial OSCC growth by inducing cell cycle arrest, apoptosis, and the migration and invasion of OSCC cells. We also demonstrated that CsA could inhibit the expression of NFATc1 and its downstream genes COX-2, c-Myc, MMP-9, and MMP-2 in OSCC cells. Furthermore, we analyzed the expression of NFATc1 in head and neck cancer through the Oncomine database. The data was consistent with the experimental findings.Conclusion:The present study initially demonstrated that CsA could inhibit the progression of OSCC cells and can mediate the signal molecules of NFATc1 signaling pathway, which has strong relationship with cancer development. That explains us CsA has potential to explore the possibilities as a novel chemotherapeutic drug for the treatment of OSCC.

Association between cell cycle gene transcription and tumor size in oral squamous cell carcinoma

Tumor Biology ◽  
2015 ◽  
Vol 36 (12) ◽  
pp. 9717-9722 ◽  
Author(s):  
Marina Gonçalves Diniz ◽  
Jeane de Fatima Correia Silva ◽  
Fabricio Tinôco Alvim de Souza ◽  
Núbia Braga Pereira ◽  
Carolina Cavaliéri Gomes ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Gene Transcription ◽  
Squamous Cell ◽  
Tumor Size ◽  
Cell Cycle Gene

Integrative analysis of gene alterations and immunoexpression profiles of cell cycle checkpoints in oral squamous cell carcinoma

2019 ◽  
Vol 27 (1) ◽  
pp. 95-103
Author(s):  
Mario Pérez-Sayáns ◽  
Cintia M. Chamorro-Petronacci ◽  
Alejandro I. Lorenzo-Pouso ◽  
José M. Suárez Peñaranda ◽  
José López-López ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Integrative Analysis ◽  
Cell Cycle Checkpoints ◽  
Gene Alterations

Cell cycle proteins and the development of oral squamous cell carcinoma

Oral Oncology ◽  
1999 ◽  
Vol 35 (3) ◽  
pp. 333-342 ◽  
Author(s):  
Michael L Schoelch ◽  
Joseph A Regezi ◽  
Nusi P Dekker ◽  
Irene O.L Ng ◽  
Alex McMillan ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cell Cycle Proteins

scholarly journals Apigenin impairs oral squamous cell carcinoma growth in vitro inducing cell cycle arrest and apoptosis

2013 ◽  
Vol 43 (5) ◽  
pp. 1675-1682 ◽  
Author(s):  
DANIELE MAGGIONI ◽  
WERNER GARAVELLO ◽  
ROBERTA RIGOLIO ◽  
LORENZO PIGNATARO ◽  
RENATO GAINI ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Cycle Arrest ◽  
Squamous Cell ◽  
Cycle Arrest
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