“In-bone” Utricle Cultures—A Simplified, Atraumatic Technique for In Situ Cultures of the Adult Mouse (Mus musculus) Utricle

2013 ◽  
Vol 34 (2) ◽  
pp. 353-359 ◽  
Author(s):  
Henry C. Ou ◽  
Vincent Lin ◽  
Edwin W. Rubel
Keyword(s):  
Mus Musculus ◽  
Adult Mouse

scholarly journals Aint/Tacc3 Is Highly Expressed in Proliferating Mouse Tissues During Development, Spermatogenesis, and Oogenesis

2003 ◽  
Vol 51 (4) ◽  
pp. 455-469 ◽  
Author(s):  
Marjo Aitola ◽  
Christine M. Sadek ◽  
Jan-Åke Gustafsson ◽  
Markku Pelto-Huikko
Keyword(s):  
Adult Mouse ◽  
Coiled Coil ◽  
Proliferating Cells ◽  
Helix Loop Helix ◽  
Aryl Hydrocarbon ◽  
Spatiotemporal Expression ◽  
Mouse Tissues ◽  
Murine Homologue

Aint was originally identified on the basis of its interaction in vitro with the aryl hydrocarbon nuclear receptor translocator (Arnt). Arnt is a common heterodimerization partner in the basic helix-loop–helix (bHLH)-PER-ARNT-SIM (PAS) protein family and is involved in diverse biological functions. These include xenobiotic metabolism, hypoxic response, and circadian rhythm. In addition, Arnt has a crucial role during development. Aint is a member of a growing family of transforming acidic coiled-coil (TACC) proteins and is the murine homologue of human TACC3. Here we report the spatiotemporal expression of Tacc3 mRNA and protein in embryonic, postnatally developing, and adult mouse tissues using in situ hybridization and immunocytochemistry. Tacc3 mRNA was highly expressed in proliferating cells of several organs during murine development. However, the only adult tissues expressing high levels were testis and ovary. Immunocytochemistry revealed that Tacc3 is a nuclear protein. Our results suggest that Tacc3 has an important role in murine development, spermatogenesis, and oogenesis.

scholarly journals Biosynthesis and in vivo localization of the decapentaplegic-Vg-related protein, DVR-6 (bone morphogenetic protein-6).

1993 ◽  
Vol 120 (2) ◽  
pp. 493-502 ◽  
Author(s):  
N A Wall ◽  
M Blessing ◽  
C V Wright ◽  
B L Hogan
Keyword(s):  
Translational Regulation ◽  
Adult Mouse ◽  
Protein Localization ◽  
Cell Types ◽  
Morphogenetic Protein ◽  
Mouse Tissues ◽  
Bronchiolar Epithelium ◽  
Bone Morphogenetic Protein 6

DVR-6 (BMP-6 or Vgr-1) is a member of the TGF-beta superfamily of polypeptide signaling molecules. In situ hybridization studies have previously shown that DVR-6 RNA is expressed in a variety of cell types in the mouse embryo, but no information has been available on protein localization and biosynthesis. We have produced a polyclonal antibody to the proregion of DVR-6 and used it to localize the protein in whole mount and sectioned embryonic, newborn, and adult mouse tissues. DVR-6 protein is expressed in the mouse nervous system beginning at 9.5 days postcoitum (d.p.c.) and continues through adulthood. A variety of epithelial tissues also produce DVR-6 protein, including the suprabasal layer of the skin, bronchiolar epithelium, and the cornea. Additionally, a stably transfected cell line, BMGE+H/D6c4, is used to study the biosynthesis of DVR-6 protein and evidence is presented for translational regulation of DVR-6 expression.

TEX101 is shed from the surface of sperm located in the caput epididymidis of the mouse

Zygote ◽  
2005 ◽  
Vol 13 (4) ◽  
pp. 325-333 ◽  
Author(s):  
Takeshi Takayama ◽  
Takuya Mishima ◽  
Miki Mori ◽  
Tomoko Ishikawa ◽  
Takami Takizawa ◽  
...  
Keyword(s):  
Adult Mouse ◽  
Epididymal Sperm ◽  
Cell Surfaces ◽  
Testicular Sperm ◽  
Additional Information ◽  
Reactive Protein ◽  
Surface Molecules ◽  
Specific Manner ◽  
Caput Epididymidis

It is generally believed that cell-to-cell cross-talk and signal transduction are mediated by cell surface molecules that play diverse and important regulatory roles in spermatogenesis and fertilization. Recently, we identified a novel plasma membrane-associated protein, TES101-reactive protein (TES101RP, or TEX101), on mouse testicular germ cells. In this study, we investigate Tex101 mRNA expression in the adult mouse testis using in situ hybridization, and we examine the fate of TEX101 during sperm transport by immunohistochemical and Western blot analyses. Tex101 mRNA was expressed in a stage-specific manner in spermatocytes and in step 1–9 spermatids of the testis, but not in spermatogonia. Although the TEX101 protein remained on the cell surfaces of step 10–16 spermatids and testicular sperm, it was shed from epididymal sperm located in the caput epididymidis. The results of this study provide additional information on germ cell-specific TEX101 expression during spermatogenesis and post-testicular sperm maturation.

scholarly journals DNase I nick translation in situ on meiotic chromosomes of the mouse, Mus musculus

1988 ◽  
Vol 90 (4) ◽  
pp. 629-634
Author(s):  
R. Raman ◽  
A.P. Singh ◽  
I. Nanda
Keyword(s):  
Sex Chromosomes ◽  
Mus Musculus ◽  
Meiotic Prophase ◽  
Cell Types ◽  
Dnase I ◽  
Translation Method ◽  
Nick Translation ◽  
Meiotic Chromosomes

DNase-I-sensitive sites have been located on the meiotic chromosomes of the mouse, Mus musculus, by the in situ DNase I nick-translation method. We find that: (1) of all the cell types studied, pachytene nuclei are the most sensitive to DNase I; (2) in diplotene the nicks occur preferentially in the vicinity of chiasmata; (3) the sex chromosomes are also sensitive to the enzyme despite their transcriptional quiescence; and (4) in the sex bivalent the nicks are primarily observed in the putative region of recombination. We conclude that, in addition to discriminating between the transcriptionally active and inactive states of chromatin, DNase I identifies recombination-specific chromatin changes in meiotic prophase.

218. Expression of Wsb2 in the mouse testis

2005 ◽  
Vol 17 (9) ◽  
pp. 84
Author(s):  
M. Sarraj ◽  
P. J. McClive ◽  
K. L. Loveland ◽  
A. H. Sinclair
Keyword(s):  
Sertoli Cells ◽  
Leydig Cells ◽  
Adult Mouse ◽  
In Situ Hybridisation ◽  
Mouse Testis ◽  
Male Gonad ◽  
Whole Mount ◽  
Mantle Layer ◽  
Pachytene Spermatocytes

We present a detailed study on the expression pattern of Wsb2 in the mouse foetal and adult gonad. Wsb2 expression was analysed during mouse embryogenesis by whole-mount, section in situ hybridisation and immunohistochemistry. Wsb2 was found to be expressed in the developing mouse gonads from 11.5 dpc to 16.5 dpc. Expression is initially equal in both sexes from 10.5 dpc until 12.0 dpc, then it persists in the male gonad. Wsb2 expression was confined to the cords in both Sertoli cell and germ cells. Other sites of Wsb2 embryonic expression were the somites, dorsal root ganglia and the lateral mantle layer of the neural tube. mRNA encoding Wsb2 and Wsb2 protein has been detected in the newborn testis in both gonocytes and Sertoli cells. Wsb2 mRNA in the adult mouse testis was observed in Sertoli cells, spermatogonia, spermatocytes and the corresponding Wsb2 protein expression was in pachytene spermatocytes, round and elongated spermatids, Sertoli cells and Leydig cells. The differential expression of Wsb2 in male versus female embryonic gonads suggests it may play a role in mammalian sex determination during embryonic development and its expression in the first wave of spermatogenesis and in the adult suggests a later role in spermatogenesis.

Positional and developmental regulation of glutamine synthetase expression in mouse liver

1988 ◽  
Vol 8 (11) ◽  
pp. 4966-4971
Author(s):  
C F Kuo ◽  
K E Paulson ◽  
J E Darnell
Keyword(s):  
Glutamine Synthetase ◽  
Single Cell ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Cell Layer ◽  
Developmental Regulation ◽  
Cell Lineage ◽  
Specific Gene ◽  
Adult Mouse Liver

In situ hybridization showed that all fetal hepatocytes contain glutamine synthetase (GS) mRNA but that in adult mouse liver, only a single cell layer surrounding the central veins contains GS mRNA. A shift from the fetal to the adult pattern begins within a few days of birth and is complete within 12 days of birth. Since the total GS mRNA and the transcription rate of the single GS gene are similar at birth and in adults, we conclude that there is a generalized reduction in GS transcription for most hepatocytes and a sharp rise in GS transcription for the immediate pericentral cells. This may be a case of positional regulation of specific gene transcription in apparently a single cell lineage.

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