The Study on Telomere Length for Age Estimation in a Thai Population

The history of elasmobranch ageing highlights the difficulty of age estimation in animals lacking ossified structures. Ageing techniques are numerous, yet all are limited by difficulties of validation and verification and inherent subjectivity. Sample scarcity further hinders progress as limited samples cannot be risked on exploratory techniques. This study explored the use of genetic analysis of telomeres as a method of age determination in a deep-sea shark, Etmopterus granulosus, a representative of a poorly studied taxon with regard to ageing due to sample scarcity. Telomeres were amplified in 39 individuals with qPCR, compared across samples, and were found to differ across size classes. More work is needed to determine whether telomeres consistently correlate with size class across elasmobranchs. However, telomeres offer easily obtainable data that can be combined with traditional methods to provide additional points of comparison for elasmobranch biology. Our results underscore the importance of expanding current ageing methods and highlight a necessary distinction between chronological and biological ages when describing elasmobranch populations.

Download Full-text

New models for age estimation and assessment of their accuracy using developing mandibular third molar teeth in a Thai population

International Journal of Legal Medicine ◽

10.1007/s00414-016-1467-4 ◽

2016 ◽

Vol 131 (2) ◽

pp. 559-568 ◽

Cited By ~ 14

Author(s):

P. Duangto ◽

A. Iamaroon ◽

S. Prasitwattanaseree ◽

P. Mahakkanukrauh ◽

A. Janhom

Keyword(s):

Age Estimation ◽

Third Molar ◽

Thai Population ◽

Mandibular Third Molar ◽

New Models ◽

Molar Teeth

Download Full-text

Estimating age from digital radiographic images of lumbar vertebrae in a Thai population using an image analysis technique

Medicine Science and the Law ◽

10.1177/00258024211062027 ◽

2021 ◽

pp. 002580242110620

Author(s):

Yanumart Malatong ◽

Patison Palee ◽

Apichat Sinthubua ◽

Sakarat Na Lampang ◽

Pasuk Mahakkanukrauh

Keyword(s):

Image Analysis ◽

Linear Regression ◽

Age Estimation ◽

Lumbar Vertebrae ◽

Lumbar Vertebra ◽

Analysis Method ◽

Image Analysis Method ◽

Thai Population ◽

Radiographic Images ◽

Black Pixel

Using the lumbar vertebra for age estimation is helpful in cases when skeletal remains are incomplete and typical skeletal age indicators are absent. This study aimed to apply an image analysis method in extracting black pixel variables for age estimation by using the radiographic images of lumbar vertebra in a Thai population. All lumbar vertebrae L1–L5 of 220 (110 males and 110 females) from Thai individuals of known sex and ages were studied. The variables of Total Percentage of black pixels (TP), Mean Percentage of black pixels (MP), and Ratio of black to white pixels (BW), were calculated to assess the relationship between black pixel variables and aging. Equations were formulated using linear regression analysis. The results of this study indicated three variables of the lumbar vertebrae had significantly positive correlations with age. The correlation between parameters with age in males ranged 0.211–0.419, while the range in females was 0.219–0.458. The appropriate linear regression equation with the total and mean percentages of black pixel variables shows Age = −1.348+0.871 (TP) +0.514 (MP) of L4 for males (SEE; 15.4 years), and Age = 5.338 +0.316 (TP) +0.952 (MP) of L1 for females (SEE; 13.8 years). Age estimation using an image analysis method is an alternative to investigating the trabecular structure. The black pixel variable is not the actual value of bone density. However, it is useful to study its relationship with aging.

Download Full-text

Telomere length and signal joint T-cell receptor rearrangement excision circles as biomarkers for chronological age estimation

Biomarkers ◽

10.1080/1354750x.2020.1871412 ◽

2021 ◽

pp. 1-23

Author(s):

Mostafa Ali Elmadawy ◽

Omnia A Abdulah; ◽

Walaa Bayoumie El Gazzar; ◽

Enas Sebaey Ahmad; ◽

Seham Gouda Ameen; ◽

...

Keyword(s):

T Cell ◽

Telomere Length ◽

T Cell Receptor ◽

Age Estimation ◽

Cell Receptor ◽

Chronological Age ◽

Excision Circles

Download Full-text

Age estimation by telomere length by Rt PCR method in south Delhi Random Population-a pilot study

International Journal of Medical Toxicology & Legal Medicine ◽

10.5958/0974-4614.2021.00028.0 ◽

2021 ◽

Vol 24 (1and2) ◽

pp. 160-163

Author(s):

Vivek Kumar ◽

Ajay Parkash ◽

Prateek Pandya ◽

Deepak R. Bharti ◽

Anupuma Raina

Keyword(s):

Pilot Study ◽

Telomere Length ◽

Age Estimation ◽

Rt Pcr ◽

Pcr Method

Download Full-text

Age Estimation from Computed Tomography of Cranial Suture Closure in a Thai Population

Medicine & Health ◽

10.17576/mh.2020.1502.24 ◽

2020 ◽

Vol 15 (2) ◽

pp. 276-289

Author(s):

Pasuk Mahakkanukrauh ◽

Keyword(s):

Computed Tomography ◽

Age Estimation ◽

Ct Images ◽

Ct Image ◽

Cranial Suture ◽

Thai Population ◽

Age Related ◽

Fundamental Information ◽

Starting Point ◽

Suture Closure

Age estimation from human skeletal remains is an important step to reconstruct a biological profile. Cranial suture has long been studied for its age-related closure. However, until now, forensic anthropologists still attempt to investigate the best way of estimating age at death from cranial suture closure because skull is usually found at the crime scene due to its easy recognised-appearance and persistence to post-mortem insults. For these reasons, a study of age estimation from cranial suture closure in a Thai population was conducted, which focussed to study the appearance and visibility of facial suture closure using computed tomography (CT). CT image series of 140 cases were obtained in order to investigate ectocranial closure of the selected facial sutures. The results from CT image analysis revealed that nasomaxillary provided the most consistent examination of suture closure (52%) while frontonasal delivered the lowest consistency in suture closure examination (29%). The inconsistency mostly occurred in assigning the closure score of 1 and 2. Thus, it could be suggested that a 3-scale scoring system of closure: open, closing, and closed, could be an appropriate method of evaluating degree of ectocranial closure of facial sutures obtained from CT imaging. This fundamental information of facial suture closure from CT images could serve as a starting point on development of age estimation technique from suture closure by utilising CT images.

Download Full-text

Age Estimation using Aorta Image Analysis in the Thai Population

Sains Malaysiana ◽

10.17576/jsm-2021-5002-13 ◽

2021 ◽

Vol 50 (2) ◽

pp. 419-428

Author(s):

Pornhatai Komutrattananont ◽

Treerat Gumpangseth ◽

Parison Palee ◽

Sukon Prasitwattanaseree ◽

Norzana Abd Ghafar ◽

...

Keyword(s):

Image Analysis ◽

Standard Error ◽

Age Estimation ◽

Structural Changes ◽

Ascending Aorta ◽

Morphometric Parameters ◽

Thai Population ◽

Horizontal Diameter ◽

Analysis Programme ◽

Outer Circumference

The current technology used in imaging has an increasing role in medical education. The concept of using an image analysis programme to help in estimating the age of the aorta, the largest artery, can be clearly seen to be of use, especially in establishing age changes for the identification of individuals. The purposes of the study were to investigate gross morphology, morphometric parameters, and the correlation between parameters with age of the aorta in human cadavers by using an image analysis programme. A total of 244 specimens were dissected from 4 locations of 61 human aortas from donour and autopsy cadavers with an age range of 20 to 90 years. The morphometric parameters included outer circumference, inner circumference, aortic diameter, and average thickness of all locations of the aorta. These were defined and measured by using the image analysis programme. The results of this study showed that the outer circumference (r=0.808-0.739), inner circumference (r=0.763-0.682), vertical diameter of the aorta (r=0.628-0.524) and horizontal diameter of the aorta (r=0.808-0.396) showed a significant correlation with age in all areas of the aorta. In the ascending aorta, the correlation between the outer circumference, horizontal diameter and age were higher than other variables and areas. The age equation established pertinent to the outer circumference (OC) in the ascending aorta was age = -61.648+ (1.141(OC)) with a standard error of ± 12.45 years. The age prediction equation for the horizontal diameter (HD) in the ascending aorta was age = -35.799+ (4.134(HD)) and a standard error of ± 12.43 years. In conclusion, knowledge of the structural changes associated with age can be used for age estimation in forensic anthropology.

Download Full-text

Age relationships with telomere length, body weight and body length in wild dugong (Dugong dugon)

PeerJ ◽

10.7717/peerj.10319 ◽

2020 ◽

Vol 8 ◽

pp. e10319

Author(s):

Phaothep Cherdsukjai ◽

Kittisak Buddhachat ◽

Janine Brown ◽

Manthanee Kaewkool ◽

Anocha Poommouang ◽

...

Keyword(s):

Body Weight ◽

Telomere Length ◽

Growth Rates ◽

Age Estimation ◽

Telomere Attrition ◽

Growth Status ◽

Alternative Approach ◽

Basic Biology ◽

Layer Groups ◽

Free Ranging

The ability to estimate age and determine the growth status of free-ranging dugongs (Dugong dugon) is vital to providing insight into the basic biology of this endangered species. Currently, age estimation in dugong carcasses relies on counting dentin growth layer groups (GLGs) in tusks, but a disadvantage is they need to be intact. We explored whether measures of telomere length could be used as an alternative approach to age estimation in dugongs given that in other species, telomere length and age are inversely related. In this study, relative telomere length (rTL) was measured by qPCR in skin samples from 24 dugongs of varying ages determined by counts of GLGs. In addition, relationships between age by GLG counts and body weight and length and were examined. Our findings indicate that age estimated by GLGs was negatively correlated with telomere length using the logistic formula with a rate of telomere attrition of approximately 0.036 rTL/year between the ages of 5–20 years. By comparison, both body weight and length were positively correlated with GLG-based age, with growth rates of ~8.8 kg/year for weight and ~3.58 cm/year for length, respectively. After that, growth rates slowed substantially and then plateaued. The results suggest that physical maturity in dugongs occurs at 20 years of age and that measures of rTL might serve as a tool for age estimation in dugongs, living and deceased.

Download Full-text