Clinical Safety of Stromal Vascular Fraction Separation at the Point of Care

2015 ◽  
Vol 75 (6) ◽  
pp. 666-671 ◽  
Author(s):  
Joel A. Aronowitz ◽  
Ryan A. Lockhart ◽  
Cloe S. Hakakian ◽  
Kevin C. Hicok
Keyword(s):  
Point Of Care ◽  
Stromal Vascular Fraction ◽  
Clinical Safety

scholarly journals Stromal Vascular Fraction for Osteoarthritis of the Knee Regenerative Engineering

2021 ◽  
Author(s):  
Chinedu C. Ude ◽  
Shiv Shah ◽  
Kenneth S. Ogueri ◽  
Lakshmi S. Nair ◽  
Cato T. Laurencin
Keyword(s):  
Quality Of Life ◽  
Clinical Evidence ◽  
Point Of Care ◽  
Stromal Vascular Fraction ◽  
Clinic Visit ◽  
Osteoarthritis Of The Knee ◽  
Clinical Patient ◽  
Inflammatory Proteins ◽  
Anti Inflammatory

Abstract Purpose The knee joint is prone to osteoarthritis (OA) due to its anatomical position, and several reports have implicated the imbalance between catabolic and anabolic processes within the joint as the main culprit, thus leading to investigations towards attenuation of these inflammatory signals for OA treatment. In this review, we have explored clinical evidence supporting the use of stromal vascular fraction (SVF), known for its anti-inflammatory characteristics for the treatment of OA. Methods Searches were made on PubMed, PMC, and Google Scholar with the keywords “adipose fraction knee regeneration, and stromal vascular fraction knee regeneration, and limiting searches within 2017–2020. Results Frequently found interventions include cultured adipose-derived stem cells (ADSCs), SVF, and the micronized/microfragmented adipose tissue-stromal vascular fraction (MAT-SVF). Clinical data reported that joints treated with SVF provided a better quality of life to patients. Currently, MAT-SVF obtained and administered at the point of care is approved by the Food and Drug Administration (FDA), but more studies including manufacturing validation, safety, and proof of pharmacological activity are needed for SVF. The mechanism of action of MAT-SVF is also not fully understood. However, the current hypothesis indicates a direct adherence and integration with the degenerative host tissue, and/or trophic effects resulting from the secretome of constituent cells. Conclusion Our review of the literature on stromal vascular fraction and related therapy use has found evidence of efficacy in results. More research and clinical patient follow-up are needed to determine the proper place of these therapies in the treatment of osteoarthritis of the knee. Lay Summary Reports have implicated the increased inflammatory proteins within the joints as the main cause of osteoarthritis (OA). This has attracted interest towards addressing these inflammatory proteins as a way of treatment for OA. The concentrated cell-packed portion of the adipose product stromal vascular fraction (SVF) from liposuction or other methods possesses anti-inflammatory effects and has been acclaimed to heal OA. Thus, we searched for clinical evidence supporting their use, for OA treatment through examining the literature. Data from various hospitals support that joints treated with SVF provided a better quality of life to patients. Currently, there is at least one version of these products that are obtained and given back to patients during a single clinic visit, approved by the FDA.

Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts

2013 ◽  
Vol 19 (11-12) ◽  
pp. 1295-1302 ◽  
Author(s):  
Stuart K. Williams ◽  
Paul E. Kosnik ◽  
Leigh B. Kleinert ◽  
Erik M. Vossman ◽  
Kevin D. Lye ◽  
...  
Keyword(s):  
Point Of Care ◽  
Vascular Grafts ◽  
Stromal Vascular Fraction ◽  
Expanded Polytetrafluoroethylene ◽  
Point Of Care System ◽  
Care System

scholarly journals Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Swathi SundarRaj ◽  
Abhijeet Deshmukh ◽  
Nancy Priya ◽  
Vidya S. Krishnan ◽  
Murali Cherat ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Point Of Care ◽  
Stromal Vascular Fraction ◽  
Fat Grafting ◽  
Automated System ◽  
Soft Tissue Reconstruction ◽  
Manual Method ◽  
Size Dependent ◽  
Tissue Reconstruction

Autologous fat grafting for soft tissue reconstruction is challenged by unpredictable long-term graft survival. Fat derived stromal vascular fraction (SVF) is gaining popularity in tissue reconstruction as SVF-enriched fat grafts demonstrate improved engraftment. SVF also has potential in regenerative medicine for remodeling of ischemic tissues by promoting angiogenesis. Since SVF cells do not require culture expansion, attempts are being made to develop automated devices to isolate SVF at the point of care. We report development of a closed, automated system to process up to 500 mL lipoaspirate using cell size-dependent filtration technology. The yield of SVF obtained by automated tissue digestion and filtration (1.17 ± 0.5 × 105 cells/gram) was equivalent to that obtained by manual isolation (1.15 ± 0.3 × 105;p= 0.8), and the viability of the cells isolated by both methods was greater than 90%. Cell composition included CD34+CD31− adipose stromal cells, CD34+CD31+ endothelial progenitor cells, and CD34−CD31+ endothelial cells, and their relative percentages were equivalent to SVF isolated by the manual method. CFU-F capacity and expression of angiogenic factors were also comparable with the manual method, establishing proof-of-concept for fully automated SVF isolation, suitable for use in reconstructive surgeries and regenerative medicine applications.

Comparison of Stromal Vascular Fraction and Passaged Adipose-Derived Stromal/Stem Cells as Point-of-Care Agents for Bone Regeneration

2019 ◽  
Vol 25 (21-22) ◽  
pp. 1459-1469 ◽  
Author(s):  
Ethan Nyberg ◽  
Ashley Farris ◽  
Aine O'Sullivan ◽  
Ricardo Rodriguez ◽  
Warren Grayson
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Point Of Care ◽  
Stromal Vascular Fraction ◽  
Stromal Stem Cells

scholarly journals Finding relevant free-text radiology reports at scale with IBM Watson Content Analytics: a feasibility study in the UK NHS

2019 ◽  
Vol 10 (S1) ◽  
Author(s):  
Alicja Piotrkowicz ◽  
Owen Johnson ◽  
Geoff Hall
Keyword(s):  
Feasibility Study ◽  
Large Scale ◽  
Information Overload ◽  
Point Of Care ◽  
Safety Net ◽  
Free Text ◽  
Text Analytics ◽  
Clinical Safety ◽  
Radiology Reports ◽  
Ibm Watson

Abstract Background Significant amounts of health data are stored as free-text within clinical reports, letters, discharge summaries and notes. Busy clinicians have limited time to read such large amounts of free-text and are at risk of information overload and consequently missing information vital to patient care. Automatically identifying relevant information at the point of care has the potential to reduce these risks but represents a considerable research challenge. One software solution that has been proposed in industry is the IBM Watson analytics suite which includes rule-based analytics capable of processing large document collections at scale. Results In this paper we present an overview of IBM Watson Content Analytics and a feasibility study using Content Analytics with a large-scale corpus of clinical free-text reports within a UK National Health Service (NHS) context. We created dictionaries and rules for identifying positive incidence of hydronephrosis and brain metastasis from 5.6 m radiology reports and were able to achieve 94% precision, 95% recall and 89% precision, 94% recall respectively on a sample of manually annotated reports. With minor changes for US English we applied the same rule set to an open access corpus of 0.5 m radiology reports from a US hospital and achieved 93% precision, 94% recall and 84% precision, 88% recall respectively. Conclusions We were able to implement IBM Watson within a UK NHS context and demonstrate effective results that could provide clinicians with an automatic safety net which highlights clinically important information within free-text documents. Our results suggest that currently available technologies such as IBM Watson Content Analytics already have the potential to address information overload and improve clinical safety and that solutions developed in one hospital and country may be transportable to different hospitals and countries. Our study was limited to exploring technical aspects of the feasibility of one industry solution and we recognise that healthcare text analytics research is a fast-moving field. That said, we believe our study suggests that text analytics is sufficiently advanced to be implemented within industry solutions that can improve clinical safety.

scholarly journals Autologous stromal vascular fraction therapy for rheumatoid arthritis: rationale and clinical safety

2012 ◽  
Vol 5 (1) ◽  
pp. 5 ◽  
Author(s):  
Jorge Paz Rodriguez ◽  
Michael P Murphy ◽  
Soonjun Hong ◽  
Marialaura Madrigal ◽  
Keith L March ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Stromal Vascular Fraction ◽  
Clinical Safety

948: Clinical Safety, Histologic Findings and Surgical Methods in Complex Phalloplasty Using Type I Collagen

2007 ◽  
Vol 177 (4S) ◽  
pp. 314-314 ◽  
Author(s):  
Joon-Yang Kim ◽  
Hoon Seog Jean ◽  
Beom Joon Kim ◽  
Kye Yong Song
Keyword(s):  
Type I Collagen ◽  
Type I ◽  
Clinical Safety ◽  
Surgical Methods

“Aspirin - resistance”? A few critical considerations on definition, terminology, diagnosis, clinical value, natural course of atherosclerotic disease, and therapeutic consequences

VASA ◽  
2011 ◽  
Vol 40 (6) ◽  
pp. 429-438 ◽  
Author(s):  
Berent ◽  
Sinzinger
Keyword(s):  
Platelet Function ◽  
Point Of Care ◽  
Aspirin Resistance ◽  
Point Of View ◽  
Evidence Based ◽  
Platelet Function Tests ◽  
Clinical Value ◽  
Vascular Events ◽  
Therapeutic Consequences ◽  
Function Tests

Based upon various platelet function tests and the fact that patients experience vascular events despite taking acetylsalicylic acid (ASA or aspirin), it has been suggested that patients may become resistant to the action of this pharmacological compound. However, the term “aspirin resistance” was created almost two decades ago but is still not defined. Platelet function tests are not standardized, providing conflicting information and cut-off values are arbitrarily set. Intertest comparison reveals low agreement. Even point of care tests have been introduced before appropriate validation. Inflammation may activate platelets, co-medication(s) may interfere significantly with aspirin action on platelets. Platelet function and Cox-inhibition are only some of the effects of aspirin on haemostatic regulation. One single test is not reliable to identify an altered response. Therefore, it may be more appropriate to speak about “treatment failure” to aspirin therapy than using the term “aspirin resistance”. There is no evidence based justification from either the laboratory or the clinical point of view for platelet function testing in patients taking aspirin as well as from an economic standpoint. Until evidence based data from controlled studies will be available the term “aspirin resistance” should not be further used. A more robust monitoring of factors resulting in cardiovascular events such as inflammation is recommended.

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