scholarly journals Three-dimensional imaging of human stem cells using soft X-ray tomography

2015 ◽  
Vol 12 (108) ◽  
pp. 20150252 ◽  
Author(s):  
J. C. Niclis ◽  
S. V. Murphy ◽  
D. Y. Parkinson ◽  
A. Zedan ◽  
A. H. Sathananthan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spatial Resolution ◽  
Three Dimensional ◽  
Surface Protein ◽  
Human Stem Cells ◽  
Three Dimensional Imaging ◽  
X Ray ◽  
Antibody Staining ◽  
Excellent Spatial Resolution ◽  
A Cell

Three-dimensional imaging of human stem cells using transmission soft X-ray tomography (SXT) is presented for the first time. Major organelle types—nuclei, nucleoli, mitochondria, lysosomes and vesicles—were discriminated at approximately 50 nm spatial resolution without the use of contrast agents, on the basis of measured linear X-ray absorption coefficients and comparison of the size and shape of structures to transmission electron microscopy (TEM) images. In addition, SXT was used to visualize the distribution of a cell surface protein using gold-labelled antibody staining. We present the strengths of SXT, which include excellent spatial resolution (intermediate between that of TEM and light microscopy), the lack of the requirement for fixative or contrast agent that might perturb cellular morphology or produce imaging artefacts, and the ability to produce three-dimensional images of cells without microtome sectioning. Possible applications to studying the differentiation of human stem cells are discussed.

High-Spatial-Resolution Three-dimensional Imaging of Human Spinal Cord and Column Anatomy with Postmortem X-ray Phase-Contrast Micro-CT

Radiology ◽  
2021 ◽  
Vol 298 (1) ◽  
pp. 135-146
Author(s):  
Giacomo E. Barbone ◽  
Alberto Bravin ◽  
Alberto Mittone ◽  
Sergio Grosu ◽  
Jens Ricke ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spatial Resolution ◽  
Phase Contrast ◽  
High Spatial Resolution ◽  
Three Dimensional ◽  
Micro Ct ◽  
Three Dimensional Imaging ◽  
X Ray ◽  
Human Spinal Cord

scholarly journals Report on a project on three-dimensional imaging of the biological cell by single-particle X-ray diffraction

2007 ◽  
Vol 64 (1) ◽  
pp. 33-35 ◽  
Author(s):  
D. Sayre
Keyword(s):  
Single Particle ◽  
High Vacuum ◽  
Three Dimensional ◽  
X Rays ◽  
X Ray Diffraction ◽  
Acta Cryst ◽  
Three Dimensional Imaging ◽  
X Ray ◽  
X Ray Crystallography ◽  
A Cell

Single-particle X-ray diffraction is an extension of X-ray crystallography which allows the specimen to be any small solid-state bounded object; in Shapiroet al.[Proc. Natl Acad. Sci. USA(2005),102, 15343–15346] and Thibaultet al.[Acta Cryst.(2006), A62, 248–261], the reader can find descriptions of a recent StonyBrook/Berkeley/Cornell two-dimensional imaging of a yeast cell by this technique. Our present work is aimed at extending the technique to the three-dimensional imaging of a cell. However, the usual method of doing that, namely rotating the specimen into many orientations in the X-ray beam, has not as yet given sufficiently good three-dimensional diffraction data to allow the work to go forward, the largest problem being the difficulty of preventing unwanted levels of change in the specimen through the extended exposure to a hostile environment of X-rays and, in some cases, high vacuum and/or extreme cold. The present paper discusses possible methods of dealing with this problem.

Three-Dimensional Imaging of Dissolution-Driven Convection in Simple Porous Media Using X-ray CT Scanning

2021 ◽  
Author(s):  
Anna-Maria Eckel ◽  
Rebecca Liyanage ◽  
Takeshi Kurotori ◽  
Ronny Pini
Keyword(s):  
Porous Media ◽  
Three Dimensional ◽  
Ct Scanning ◽  
Three Dimensional Imaging ◽  
X Ray

The Crystal Structure of KIO3.HIO3

1971 ◽  
Vol 49 (3) ◽  
pp. 468-476 ◽  
Author(s):  
Lilian Y. Y. Chan ◽  
F. W. B. Einstein
Keyword(s):  
Crystal Structure ◽  
Three Dimensional ◽  
Monoclinic Space Group ◽  
X Ray ◽  
R Factor ◽  
Octahedral Environment ◽  
Distorted Octahedral ◽  
Potassium Hydrogen ◽  
A Cell ◽  
Least Squares Techniques

The crystal structure of potassium hydrogen di-iodate (bi-iodate) KIO3.HIO3 was determined from three dimensional X-ray data collected by counter methods. The structure was refined by full-matrix least-squares techniques to a conventional R factor of 5.0 % for the 1392 observed reflexions. The salt crystallizes in the monoclinic space group P21/c with eight formula units in a cell of dimension a = 7.028(1) Å, b = 8.203(1) Å, c = 21.841(3) Å, β = 98.03(1)°.The iodate units are all basically pyramidal; weak interionic I—O contacts complete a very distorted octahedral environment around three iodine atoms. There is a capped octahedral (7-coordinate) environment around the remaining iodine atom. The I—O bonds are in the range 1.75–1.82 Å and the I—OH bonds are 1.91 and 1.95 Å, variations in length can be correlated with differences in the degree of involvement in (a) hydrogen bonding and (b) interaction with adjacent iodine atoms.

scholarly journals Three-dimensional Imaging of Nanoscale Internal Structure by Coherent X-ray Diffraction Microscope

Materia Japan ◽  
2007 ◽  
Vol 46 (12) ◽  
pp. 827-827
Author(s):  
Yoshinori Nishino ◽  
Yukio Takahashi ◽  
Tetsuya Ishikawa ◽  
Eiichiro Matsubara
Keyword(s):  
Internal Structure ◽  
Three Dimensional ◽  
X Ray Diffraction ◽  
Three Dimensional Imaging ◽  
X Ray

scholarly journals Three-dimensional imaging of chemical phase transformations at the nanoscale with full-field transmission X-ray microscopy

2011 ◽  
Vol 18 (5) ◽  
pp. 773-781 ◽  
Author(s):  
Florian Meirer ◽  
Jordi Cabana ◽  
Yijin Liu ◽  
Apurva Mehta ◽  
Joy C. Andrews ◽  
...  
Keyword(s):  
Phase Transformations ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Full Field ◽  
X Ray ◽  
Chemical Phase

Three-dimensional imaging of dislocations by X-ray diffraction laminography

2012 ◽  
Vol 101 (24) ◽  
pp. 244103 ◽  
Author(s):  
D. Hänschke ◽  
L. Helfen ◽  
V. Altapova ◽  
A. Danilewsky ◽  
T. Baumbach
Keyword(s):  
Three Dimensional ◽  
X Ray Diffraction ◽  
Three Dimensional Imaging ◽  
X Ray

scholarly journals Three-Dimensional Imaging of Nerve Tissue by X-Ray Phase-Contrast Microtomography

1999 ◽  
Vol 76 (1) ◽  
pp. 98-102 ◽  
Author(s):  
F. Beckmann ◽  
K. Heise ◽  
B. Kölsch ◽  
U. Bonse ◽  
M.F. Rajewsky ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Three Dimensional ◽  
Nerve Tissue ◽  
Three Dimensional Imaging ◽  
X Ray

Region-of-Interest X-Ray Tomography for the Non-Destructive Characterization of Local Fiber Orientation in Large Fiber Composite Parts

2019 ◽  
Vol 809 ◽  
pp. 587-593
Author(s):  
Simon Zabler ◽  
Katja Schladitz ◽  
Kilian Dremel ◽  
Jonas Graetz ◽  
Dascha Dobrovolskij
Keyword(s):  
Computed Tomography ◽  
Spatial Resolution ◽  
Fiber Orientation ◽  
Fiber Composite ◽  
Three Dimensional ◽  
Region Of Interest ◽  
Micro Computed Tomography ◽  
X Ray ◽  
Anisotropic Thermal Conductivity ◽  
Local Fiber

To detect and characterize materials defects in fiber composites as well as for evaluatingthe three-dimensional local fiber orientation in the latter, X-ray micro-CT is the preferred methodof choice. When micro computed tomography is applied to inspect large components, the method isreferred to as region-of-interest computed tomography. Parts can be as large as 10 cm wide and 1 mlong, while the measurement volume of micro computed tomography is a cylinder of only 4 − 5 mmdiameter (typical wall thickness of fiber composite parts). In this report, the potentials and limits ofregion-of-interest computed tomography are discussed with regard to spatial resolution and precisionwhen evaluating defects and local fiber orientation in squeeze cast components. The micro computedtomography scanner metRIC at Fraunhofer‘s Development Center X-ray Technology EZRT deliversregion-of-interest computed tomography up to a spatial resolution of 2 μm/voxel, which is sufficientfor determining the orientation of natural or synthetic fibers, wood, carbon and glass. The mean localfiber orientation is estimated on an isotropic structuring element of approximately 0.1 mm length bymeans of volume image analysis (MAVI software package by Fraunhofer ITWM). Knowing the exactlocal fiber orientation is critical for estimating anisotropic thermal conductivity and materials strength.

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