Correction to ‘Cytotoxicity studies of Fe
            3
            O
            4
            nanoparticles in chicken macrophage cells’

Magnetic Fe 3 O 4 nanoparticles (Fe 3 O 4 -NPs) have been widely investigated for their biomedical applications. The main purpose of this study was to evaluate the cytotoxic effects of different sizes of Fe 3 O 4 -NPs in chicken macrophage cells (HD11). Experimental groups based on three sizes of Fe 3 O 4 -NPs (60, 120 and 250 nm) were created, and the Fe 3 O 4 -NPs were added to the cells at different doses according to the experimental group. The cell activity, oxidative index (malondialdehyde (MDA), superoxide dismutase (SOD) and reactive oxygen species (ROS)), apoptosis and pro-inflammatory cytokine secretion level were detected to analyse the cytotoxic effects of Fe 3 O 4 -NPs of different sizes in HD11 cells. The results revealed that the cell viability of the 60 nm Fe 3 O 4 -NPs group was lower than those of the 120 and 250 nm groups when the same concentration of Fe 3 O 4 -NPs was added. No significant difference in MDA was observed among the three Fe 3 O 4 -NP groups. The SOD level and ROS production of the 60 nm group were significantly greater than those of the 120 and 250 nm groups. Furthermore, the highest levels of apoptosis and pro-inflammatory cytokine secretion were caused by the 60 nm Fe 3 O 4 -NPs. In conclusion, the smaller Fe 3 O 4 -NPs produced stronger cytotoxicity in chicken macrophage cells, and the cytotoxic effects may be related to the oxidative stress and apoptosis induced by increased ROS production as well as the increased expression of pro-inflammatory cytokines.

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Developmental activation of the lysozyme gene in chicken macrophage cells is linked to core histone acetylation at its enhancer elements

Nucleic Acids Research ◽

10.1093/nar/gkl543 ◽

2006 ◽

Vol 34 (14) ◽

pp. 4025-4035 ◽

Cited By ~ 9

Author(s):

Fiona A. Myers ◽

Pascal Lefevre ◽

Evangelia Mantouvalou ◽

Kimberley Bruce ◽

Claire Lacroix ◽

...

Keyword(s):

Histone Acetylation ◽

Core Histone ◽

Lysozyme Gene ◽

Macrophage Cells ◽

Chicken Macrophage

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Association of SPOP Expression with the Immune Response to Salmonella Infection in Chickens

Animals ◽

10.3390/ani10020307 ◽

2020 ◽

Vol 10 (2) ◽

pp. 307

Author(s):

Fei Wang ◽

Qinghe Li ◽

Qiao Wang ◽

Maiqing Zheng ◽

Jie Wen ◽

...

Keyword(s):

Immune Response ◽

Zinc Finger Protein ◽

Salmonella Infection ◽

Macrophage Cells ◽

Chicken Macrophage ◽

Polymerase Chain ◽

Iga Production ◽

Broad Complex

Salmonellosis is a zoonosis that is not only harmful to the health of poultry but also poses a threat to human health. Although many measures have been put in place to reduce morbidity, they have not provided satisfactory results. Therefore, it is necessary to clarify the immune mechanisms involved in improving the resistance of chickens against Salmonella. BTB (Broad-complex Tramtrack and Bric-a-brac) Speckle-type POZ (poxvirus and zinc finger) protein (SPOP) regulates protein expression by promoting substrate ubiquitination and degradation. The correlation between SPOP expression and the immune response has not been fully described. Therefore, the aim of this study was to clarify this relationship. In vitro, we stimulated chicken macrophage cells (HD11) with lipopolysaccharide, then analyzed the correlation between SPOP and IL1β or IL8 expression using quantitative real-time polymerase chain reaction (qRT-PCR). In vivo, we infected 7-days-old chickens with Salmonella Typhimurium, then analyzed the association between SPOP expression and the immune response, including IL1β and IL8 expression, IgA production, and bacterial loads. We found that SPOP may participate in the regulation of the immune response in macrophage cells. SPOP expression was negatively correlated with IL-1β and IL-8 expression both in vivo and in vitro. SPOP expression was also negatively related to bacterial loads and immunoglobulin (Ig) A production. These results indicate that SPOP may have important functions in the response to Salmonella infection.

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