scholarly journals The clonal composition of biramous and uniramous arthropod limbs

2008 ◽  
Vol 275 (1638) ◽  
pp. 1023-1028 ◽  
Author(s):  
Carsten Wolff ◽  
Gerhard Scholtz
Keyword(s):  
Single Cell ◽  
Limb Development ◽  
Cell Lineage ◽  
Growth Zone ◽  
Cell Labelling ◽  
General Belief ◽  
Developmental Approach ◽  
Orchestia Cavimana ◽  
Clonal Composition ◽  
Lineage Analysis

We present the first comparative cell lineage analysis of uniramous and biramous limbs of an arthropod, the crustacean Orchestia cavimana . Via single cell labelling of the cells that are involved in limb development, we are able to present the first complete clonal composition of an arthropod limb. We show that the two main branches of crustacean limbs, exopod and endopod, are formed by a secondary subdivision of the growth zone of the main limb axis. Additional limb outgrowths such as exites result from the establishment of new axes. In contrast to general belief, uniramous limbs in Orchestia are not formed by the loss of the exopod but by suppression of the split into exopod and endopod. Our results offer a developmental approach to discriminate between the different kinds of branches of arthropod appendages. This leads to the conclusion that a ‘true’ biramous limb comprising an endopod and an exopod might have occurred much later in euarthropod evolution than has previously been thought, probably either in the lineage of the Mandibulata or that of the Tetraconata.

scholarly journals Cell lineage and cell cycling analyses of the 4d micromere using live imaging in the marine annelid Platynereis dumerilii

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
B Duygu Özpolat ◽  
Mette Handberg-Thorsager ◽  
Michel Vervoort ◽  
Guillaume Balavoine
Keyword(s):  
Cell Cycle ◽  
Single Cell ◽  
Cell Fate ◽  
Cell Lineage ◽  
Growth Zone ◽  
Live Imaging ◽  
In Vivo Studies ◽  
Platynereis Dumerilii ◽  
Cell Cycling

Cell lineage, cell cycle, and cell fate are tightly associated in developmental processes, but in vivo studies at single-cell resolution showing the intricacies of these associations are rare due to technical limitations. In this study on the marine annelid Platynereis dumerilii, we investigated the lineage of the 4d micromere, using high-resolution long-term live imaging complemented with a live-cell cycle reporter. 4d is the origin of mesodermal lineages and the germline in many spiralians. We traced lineages at single-cell resolution within 4d and demonstrate that embryonic segmental mesoderm forms via teloblastic divisions, as in clitellate annelids. We also identified the precise cellular origins of the larval mesodermal posterior growth zone. We found that differentially-fated progeny of 4d (germline, segmental mesoderm, growth zone) display significantly different cell cycling. This work has evolutionary implications, sets up the foundation for functional studies in annelid stem cells, and presents newly established techniques for live imaging marine embryos.

scholarly journals Single-cell lineage analysis reveals genetic and epigenetic interplay in glioblastoma drug resistance

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Christine E. Eyler ◽  
Hironori Matsunaga ◽  
Volker Hovestadt ◽  
Samantha J. Vantine ◽  
Peter van Galen ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Single Cell ◽  
Cell Lineage ◽  
Lineage Analysis

scholarly journals Single-cell lineage analysis reveals extensive multimodal transcriptional control during directed beta-cell differentiation

2020 ◽  
Vol 2 (12) ◽  
pp. 1443-1458
Author(s):  
Chen Weng ◽  
Jiajia Xi ◽  
Haiyan Li ◽  
Jian Cui ◽  
Anniya Gu ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Beta Cell ◽  
Single Cell ◽  
Transcriptional Control ◽  
Cell Lineage ◽  
Lineage Analysis

The fate of the CNS midline progenitors in Drosophila as revealed by a new method for single cell labelling

Development ◽  
1994 ◽  
Vol 120 (7) ◽  
pp. 1895-1906 ◽  
Author(s):  
T. Bossing ◽  
G.M. Technau
Keyword(s):  
Single Cells ◽  
Cell Lineage ◽  
New Method ◽  
Cell Labelling ◽  
Fluorescent Tracer ◽  
Cell Divisions ◽  
Cns Midline ◽  
Midline Cells ◽  
Lineage Analysis

We present a new method for marking single cells and tracing their development through embryogenesis. Cells are labelled with a lipophilic fluorescent tracer (DiI) in their normal positions without impaling their membranes. The dye does not diffuse between cells but is transferred to the progeny, disclosing their morphology in all detail. Behaviour of labelled cells can be observed in vivo (cell divisions, morphogenetic movements and differentiation). Following photoconversion of the dye, fully differentiated clones can be analyzed in permanent preparations. We apply this method for cell lineage analysis of the embryonic Drosophila CNS. Here we describe the fate of the CNS midline cells. We present the complete lineages of these cells in the fully differentiated embryo and show that variability exists in segmental numbers of the midline progenitors as well as in the composition of their lineages.

scholarly journals Mesenchymal multipotency of adult human periosteal cells demonstrated by single-cell lineage analysis

2006 ◽  
Vol 54 (4) ◽  
pp. 1209-1221 ◽  
Author(s):  
Cosimo De Bari ◽  
Francesco Dell'Accio ◽  
Johan Vanlauwe ◽  
Jeroen Eyckmans ◽  
Ilyas M. Khan ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Lineage ◽  
Adult Human ◽  
Periosteal Cells ◽  
Lineage Analysis
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