scholarly journals Cell lineage analysis of the mandibular segment of the amphipod Orchestia cavimana reveals that the crustacean paragnaths are sternal outgrowths and not limbs

2006 ◽  
Vol 3 (1) ◽  
Author(s):  
Carsten Wolff ◽  
Gerhard Scholtz
Keyword(s):  
Cell Lineage ◽  
Mandibular Segment ◽  
Orchestia Cavimana ◽  
Lineage Analysis

scholarly journals The clonal composition of biramous and uniramous arthropod limbs

2008 ◽  
Vol 275 (1638) ◽  
pp. 1023-1028 ◽  
Author(s):  
Carsten Wolff ◽  
Gerhard Scholtz
Keyword(s):  
Single Cell ◽  
Limb Development ◽  
Cell Lineage ◽  
Growth Zone ◽  
Cell Labelling ◽  
General Belief ◽  
Developmental Approach ◽  
Orchestia Cavimana ◽  
Clonal Composition ◽  
Lineage Analysis

We present the first comparative cell lineage analysis of uniramous and biramous limbs of an arthropod, the crustacean Orchestia cavimana . Via single cell labelling of the cells that are involved in limb development, we are able to present the first complete clonal composition of an arthropod limb. We show that the two main branches of crustacean limbs, exopod and endopod, are formed by a secondary subdivision of the growth zone of the main limb axis. Additional limb outgrowths such as exites result from the establishment of new axes. In contrast to general belief, uniramous limbs in Orchestia are not formed by the loss of the exopod but by suppression of the split into exopod and endopod. Our results offer a developmental approach to discriminate between the different kinds of branches of arthropod appendages. This leads to the conclusion that a ‘true’ biramous limb comprising an endopod and an exopod might have occurred much later in euarthropod evolution than has previously been thought, probably either in the lineage of the Mandibulata or that of the Tetraconata.

Major sex-determining genes and the control of sexual dimorphism in Caenorhabditis elegans

Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 625-637 ◽  
Author(s):  
Jonathan Hodgkin ◽  
Andrew D. Chisholm ◽  
Michael M. Shen
Keyword(s):  
Caenorhabditis Elegans ◽  
Sex Determination ◽  
X Chromosome ◽  
Germ Line ◽  
Cell Lineage ◽  
Regulatory Genes ◽  
Nematode Caenorhabditis Elegans ◽  
X Chromosomes ◽  
The Many ◽  
Lineage Analysis

Sex determination in Caenorhabditis elegans involves a cascade of major regulatory genes connecting the primary sex determining signal, X chromosome dosage, to key switch genes, which in turn direct development along either male or female pathways. Animals with one X chromosome (XO) are male, while animals with two X chromosomes (XX) are hermaphrodite: hermaphrodite development occurs because the action of the regulatory genes is modified in the germ line so that both sperm and oocytes are made inside a completely female soma. The regulatory genes are being examined by both genetic and molecular means. We discuss how these major genes, in particular the last switch gene in the cascade, tra-1, might regulate the many different sex-specific events that occur during the development of the hermaphrodite and of the male.Key words: nematode, Caenorhabditis elegans, sex determination, sexual differentiation, cell lineage analysis.

Cell lineage of homeotic mutants of Drosophila

1985 ◽  
Vol 312 (1153) ◽  
pp. 139-144 ◽  
Keyword(s):  
Mode Of Action ◽  
Cell Lineage ◽  
Precursor Cells ◽  
Larval Period ◽  
Homeotic Genes ◽  
Lineage Analysis

The homeotic genes specify the development of specific groups of precursor cells. They establish the correct state of determination of the different primordia. Cell lineage analysis has been particularly useful in studying the mode of action of homeotic genes. The main findings are: (i) most, perhaps all, the homeotic genes are required by every cell of the corresponding primordium (that is, they are cell autonomous); (ii) they act on anatomical units defined by compartment boundaries and including one or more compartments, (iii) most, but not all, homeotic genes are required until the end of the larval period; (iv) the homeotic genes act in combination so that the appropriate development of a given primordium may be established by the contribution of several homeotic genes.

Cell lineage analysis in neural crest ontogeny

1993 ◽  
Vol 24 (2) ◽  
pp. 146-161 ◽  
Author(s):  
Nicole M. Le Douarin ◽  
Elisabeth Dupin
Keyword(s):  
Neural Crest ◽  
Cell Lineage ◽  
Lineage Analysis

Genetic analysis of leaf development in cotton

Development ◽  
1991 ◽  
Vol 113 (Supplement_1) ◽  
pp. 39-46 ◽  
Author(s):  
Liam Dolan ◽  
R. Scott Poethig
Keyword(s):  
Leaf Growth ◽  
Leaf Shape ◽  
Cell Lineage ◽  
Cotton Leaf ◽  
Expansion Phase ◽  
Genetic Mosaics ◽  
Developmental Age ◽  
Allometric Analysis ◽  
Marginal Region ◽  
Lineage Analysis

Leaf shape in cotton is regulated by the developmental age of the shoot and by several major genes that affect leaf lobing. The effect of these factors was investigated by allometric analysis, cell lineage analysis, and by studying the expression of the leaf shape mutation, Okra, in genetic mosaics. Allometric analysis of leaf growth suggests that leaf shape is determined during the initiation of the primordium rather than during the expansion phase of leaf growth. Clonal analysis demonstrates that both the rate and duration of cell division are fairly uniform throughout the leaf. Cells in the marginal region of the developing cotton leaf contribute more to the growth of the lamina than they do in tobacco. The Okra mutation acts early in the development of a leaf and appears to accentuate a developmental pattern that is also responsible for heteroblastic variation in leaf shape. The expression of this mutation in genetic mosaics demonstrates that its effect does not diffuse laterally within the leaf primordium.

Cell lineage analysis of Schwann cells in the PNS determined by shiverer-normal mouse chimeras

1984 ◽  
Vol 105 (1) ◽  
pp. 221-226 ◽  
Author(s):  
Katsuhiko Mikoshiba ◽  
Minesuke Yokoyama ◽  
Ken Takamatsu ◽  
Yasuzo Tsukada ◽  
Tatsuji Nomura
Keyword(s):  
Schwann Cells ◽  
Normal Mouse ◽  
Cell Lineage ◽  
Mouse Chimeras ◽  
Lineage Analysis

scholarly journals Establishment of signaling interactions with cellular resolution for every cell cycle of embryogenesis

2018 ◽  
Author(s):  
Long Chen ◽  
Vincy Wing Sze Ho ◽  
Ming-Kin Wong ◽  
Xiaotai Huang ◽  
Lu-yan Chan ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Notch Signaling ◽  
Cell Lineage ◽  
Cellular Interaction ◽  
Cell Contact ◽  
Contact Map ◽  
C Elegans ◽  
Cellular Resolution ◽  
Signaling Interactions ◽  
Lineage Analysis

AbstractIntercellular signaling interaction plays a key role in breaking fate symmetry during animal development. Identification of the signaling interaction at cellular resolution is technically challenging, especially in a developing embryo. Here we develop a platform that allows automated inference and validation of signaling interaction for every cell cycle of C. elegans embryogenesis. This is achieved by generation of a systems-level cell contact map that consists of 1,114 highly confident intercellular contacts by modeling analysis and is validated through cell membrane labeling coupled with cell lineage analysis. We apply the map to identify cell pairs between which a Notch signaling interaction takes place. By generating expression patterns for two ligands and two receptors of Notch signaling pathway with cellular resolution using automated expression profiling technique, we are able to refine existing and identify novel Notch interactions during C. elegans embryogenesis. Targeted cell ablation followed by cell lineage analysis demonstrates the roles of signaling interactions over cell division in breaking fate symmetry. We finally develop a website that allows online access to the cell-cell contact map for mapping of other signaling interaction in the community. The platform can be adapted to establish cellular interaction from any other signaling pathways.

Retroviral Cell Lineage Analysis in the Developing Chick Heart

2003 ◽  
pp. 297-304
Author(s):  
Robert G. Gourdie ◽  
Gang Cheng ◽  
Robert P. Thompson ◽  
Takashi Mikawa
Keyword(s):  
Cell Lineage ◽  
Chick Heart ◽  
Lineage Analysis

The Drosophila mushroom body is a quadruple structure of clonal units each of which contains a virtually identical set of neurones and glial cells

Development ◽  
1997 ◽  
Vol 124 (4) ◽  
pp. 761-771 ◽  
Author(s):  
K. Ito ◽  
W. Awano ◽  
K. Suzuki ◽  
Y. Hiromi ◽  
D. Yamamoto
Keyword(s):  
Glial Cells ◽  
Sensory Integration ◽  
Mushroom Body ◽  
Expression Patterns ◽  
Cell Lineage ◽  
Cell Types ◽  
Enhancer Trap ◽  
Adult Brain ◽  
A New Technique ◽  
Lineage Analysis

The mushroom body (MB) is an important centre for higher order sensory integration and learning in insects. To analyse the development and organisation of the MB neuropile in Drosophila, we performed cell lineage analysis in the adult brain with a new technique that combines the Flippase (flp)/FRT system and the GAL4/UAS system. We showed that the four mushroom body neuroblasts (MBNbs) give birth exclusively to the neurones and glial cells of the MB, and that each of the four MBNb clones contributes to the entire MB structure. The expression patterns of 19 GAL4 enhancer-trap strains that mark various subsets of MB cells revealed overlapping cell types in all four of the MBNb lineages. Partial ablation of MBNbs using hydroxyurea showed that each of the four neuroblasts autonomously generates the entire repertoire of the known MB substructures.

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