Asymmetries in the cilia of
            Chlamydomonas

The generation of ciliary waveforms requires the spatial and temporal regulation of dyneins. This review catalogues many of the asymmetric structures and proteins in the cilia of Chlamydomonas , a unicellular alga with two cilia that are used for motility in liquid medium. These asymmetries, which have been identified through mutant analysis, cryo-EM tomography and proteomics, provide a wealth of information to use for modelling how waveforms are generated and propagated. This article is part of the Theo Murphy meeting issue ‘Unity and diversity of cilia in locomotion and transport’.

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EMCAT: An automatic image-processing system for electron-microscopic tomography

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100169821 ◽

1994 ◽

Vol 52 ◽

pp. 418-419

Author(s):

Weiping Liu ◽

John W. Sedat ◽

David A. Agard

Keyword(s):

Image Processing ◽

Structural Information ◽

Imaging Technique ◽

Three Dimensional ◽

Processing System ◽

Electron Microscopic ◽

Data Set ◽

Ordered Structures ◽

Automatic Image Processing ◽

Em Tomography

Any real world object is three-dimensional. The principle of tomography, which reconstructs the 3-D structure of an object from its 2-D projections of different view angles has found application in many disciplines. Electron Microscopic (EM) tomography on non-ordered structures (e.g., subcellular structures in biology and non-crystalline structures in material science) has been exercised sporadically in the last twenty years or so. As vital as is the 3-D structural information and with no existing alternative 3-D imaging technique to compete in its high resolution range, the technique to date remains the kingdom of a brave few. Its tedious tasks have been preventing it from being a routine tool. One keyword in promoting its popularity is automation: The data collection has been automated in our lab, which can routinely yield a data set of over 100 projections in the matter of a few hours. Now the image processing part is also automated. Such automations finish the job easier, faster and better.

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Somatic embryogenesis of Cyclamen persicum in liquid medium

Physiologia Plantarum ◽

10.1034/j.1399-3054.1995.940410.x ◽

1995 ◽

Vol 94 (4) ◽

pp. 605-612 ◽

Cited By ~ 3

Author(s):

Marc Kreuger ◽

Erik Postma ◽

Yvon Brouwer ◽

Gerrit-Jan van Holst

Keyword(s):

Somatic Embryogenesis ◽

Liquid Medium ◽

Cyclamen Persicum

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Melatonin: a novel strategy for prevention of obesity and fat accumulation in peripheral organs through the improvements of circadian rhythms and antioxidative capacity

Melatonin Research ◽

10.32794/mr11250048 ◽

2020 ◽

Vol 3 (1) ◽

pp. 58-76 ◽

Cited By ~ 1

Author(s):

Bohan Rong ◽

Qiong Wu ◽

Chao Sun

Keyword(s):

Oxidative Stress ◽

Skeletal Muscle ◽

Circadian Rhythms ◽

Regulatory Mechanisms ◽

Antioxidative Capacity ◽

Unicellular Alga ◽

Peripheral Tissues ◽

Peripheral Organs ◽

Regulatory Effects ◽

Novel Strategy

Melatonin is a well-known molecule for its involvement in circadian rhythm regulation and its contribution to protection against oxidative stress in organisms including unicellular alga, animals and plants. Currently, the bio-regulatory effects of melatonin on the physiology of various peripheral tissues have drawn a great attention of scientists. Although melatonin was previously defined as a neurohormone secreted from pineal gland, recently it has been identified that virtually, every cell has the capacity to synthesize melatonin and the locally generated melatonin has multiple pathophysiological functions, including regulations of obesity and metabolic syndromes. Herein, we focus on the effects of melatonin on fat deposition in various peripheral organs/tissues. The two important regulatory mechanisms related to the topic, i.e., the improvements of circadian rhythms and antioxidative capacity will be thoroughly discussed since they are linked to several biomarkers involved in obesity and energy imbalance, including metabolism and immunity. Furthermore, several other functions of melatonin which may serve to prevent or promote obesity and energy dysmetabolism-induced pathological states are also addressed. The organs of special interest include liver, pancreas, skeletal muscle, adipose tissue and the gut microbiota.

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