scholarly journals Characterization of the Type Strain of Campylobacter coli, CIP 70.80, by Plasmid Typing

1995 ◽  
Vol 45 (3) ◽  
pp. 592-594 ◽  
Author(s):  
V. STONNET ◽  
C. HARMANT ◽  
C. BIZET ◽  
J.-L. GUESDON
Keyword(s):  
Type Strain ◽  
Campylobacter Coli

Identification and characterization of genes required for post-translational modification of Campylobacter coli VC167 flagellin

1996 ◽  
Vol 19 (2) ◽  
pp. 369-378 ◽  
Author(s):  
Patricia Guerry ◽  
Peter Doig ◽  
Richard A. Alm ◽  
Donald H. Burr ◽  
Niamh Kinsella ◽  
...  
Keyword(s):  
Campylobacter Coli ◽  
Post Translational Modification ◽  
Identification And Characterization

scholarly journals Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide

2020 ◽  
Vol 21 (9) ◽  
pp. 3170
Author(s):  
Mikhail M. Shneider ◽  
Anna A. Lukianova ◽  
Peter V. Evseev ◽  
Anna M. Shpirt ◽  
Marsel R. Kabilov ◽  
...  
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Negative Impact ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Narrow Host Range ◽  
Isolation And Characterization ◽  
Control Application ◽  
Hydrolytic Mechanism

Phytopathogenic bacteria belonging to the Pectobacterium and Dickeya genera (soft-rot Pectobacteriaceae) are in the focus of agriculture-related microbiology because of their diversity, their substantial negative impact on the production of potatoes and vegetables, and the prospects of bacteriophage applications for disease control. Because of numerous amendments in the taxonomy of P. carotovorum, there are still a few studied sequenced strains among this species. The present work reports on the isolation and characterization of the phage infectious to the type strain of P. carotovorum. The phage Arno 160 is a lytic Podovirus representing a potential new genus of the subfamily Autographivirinae. It recognizes O-polysaccahride of the host strain and depolymerizes it in the process of infection using a rhamnosidase hydrolytic mechanism. Despite the narrow host range of this phage, it is suitable for phage control application.

Characterization of Exochelins of theMycobacterium bovis Type Strain and BCG Substrains

1999 ◽  
Vol 67 (4) ◽  
pp. 2035-2039
Author(s):  
Jovana Gobin ◽  
Diane K. Wong ◽  
Bradford W. Gibson ◽  
Marcus A. Horwitz
Keyword(s):  
Type Strain

Production and Characterization of a Monoclonal Antibody to Campylobacter jejuni

2009 ◽  
Vol 72 (4) ◽  
pp. 870-875 ◽  
Author(s):  
S. A. HEO ◽  
R. NANNAPANENI ◽  
M. G. JOHNSON ◽  
J. S. PARK ◽  
K. H. SEO
Keyword(s):  
Monoclonal Antibody ◽  
Campylobacter Jejuni ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Campylobacter Coli ◽  
Carbonate Buffer ◽  
Horse Blood ◽  
Microaerobic Conditions ◽  
Immunoglobulin Subclass

Campylobacter species are a group of spiral-shaped bacteria that can cause disease in humans and animals. We developed a high-affinity monoclonal antibody (MAb) probe that recognizes Campylobacter jejuni cells. Cell suspensions grown under microaerobic conditions at 42°C for 20 h on Bolton agar plates with lysed horse blood were used as live and heat-killed preparations, centrifuged at 8,000 × g for 20 min, and resuspended in carbonate buffer (pH 9.6) for coating on the enzyme-linked immunosorbent assay plates. BALB/c mice were immunized with C. jejuni sonicated cells at 107 CFU/ml to generate MAb-producing hybridoma clones. Of about 500 initial hybridoma clones, MAb 33D2, which reacted with C. jejuni and Campylobacter coli, was selected for further evaluation. MAb 33D2 is in the immunoglobulin subclass G2a and had relatively weaker reactivity with the C. coli strains tested. MAb 33D2 did not show any cross-reactions with the nine non-Campylobacter bacteria tested in the enzyme-linked immunosorbent assay and had a stronger affinity for C. jejuni as live versus heat-killed cells. In Western blot assays, MAb 33D2 recognized two major antigens of 62 and 43 kDa in extracts from C. jejuni cells but only one antigen of 62 kDa in extracts from C. coli cells.

Tomato bushy stunt virus from Prunus avium. II. Serological typing and the characterization of antibody types and activities

1968 ◽  
Vol 46 (3) ◽  
pp. 229-233 ◽  
Author(s):  
Wayne R. Allen
Keyword(s):  
Type Strain ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Tomato Bushy Stunt Virus ◽  
Cross Reactivity ◽  
Primary Response ◽  
Maximum Value ◽  
Serological Typing ◽  
Maximum Titer

Serological comparisons between the type strain of tomato bushy stunt virus and a strain from sweet cherry demonstrated that the degree of relationship was highly dependent upon the time of bleeding during the primary response. Cross-reactivity decreased after injection until just before the maximum titer was reached, and then gradually increased until the maximum value was attained. The increase in cross-reactivity was due, mainly, to a decline in synthesis and (or) release of specific antibodies. Sera fractionation revealed specific and cross-reactive antibodies among both light and heavy globulins. The cross-reactivity of light antibodies increased from the time of injection, but it remained nearly constant for heavy antibodies. Immunoelectrophoresis associated the light and heavy antibodies with IgG and IgM globulins, respectively.

scholarly journals Mycobacterium celeriflavum sp. nov., a rapidly growing scotochromogenic bacterium isolated from clinical specimens

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 510-515 ◽  
Author(s):  
Abdolrazagh Hashemi Shahraki ◽  
Cengiz Çavuşoğlu ◽  
Emanuele Borroni ◽  
Parvin Heidarieh ◽  
Orhan Kaya Koksalan ◽  
...  
Keyword(s):  
Type Strain ◽  
Type Species ◽  
Sequence Similarity ◽  
Housekeeping Genes ◽  
Mycolic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Polyphasic Characterization

Six strains of a rapidly growing scotochromogenic mycobacterium were isolated from pulmonary specimens of independent patients. Biochemical and cultural tests were not suitable for their identification. The mycolic acid pattern analysed by HPLC was different from that of any other mycobacterium. Genotypic characterization, targeting seven housekeeping genes, revealed the presence of microheterogeneity in all of them. Different species were more closely related to the test strains in various regions: the type strain of Mycobacterium moriokaense showed 99.0 % 16S rRNA gene sequence similarity, and 91.5–96.5 % similarity for the remaining six regions. The whole genome sequences of the proposed type strain and that of M. moriokaense presented an average nucleotide identity (ANI) of 82.9 %. Phylogenetic analysis produced poorly robust trees in most genes with the exception of rpoB and sodA where Mycobacterium flavescens and Mycobacterium novocastrense were the closest species. This phylogenetic relatedness was confirmed by the tree inferred from five concatenated genes, which was very robust. The polyphasic characterization of the test strains, supported by the ANI value, demonstrates that they belong to a previously unreported species, for which the name Mycobacterium celeriflavum sp. nov. is proposed. The type strain is AFPC-000207T ( = DSM 46765T = JCM 18439T).

PHENOTYPIC CHARACTERIZATION OF BENECKEA PARAHAEMOLYTICA: A PRELIMINARY REPORT1

1973 ◽  
Vol 36 (4) ◽  
pp. 214-219 ◽  
Author(s):  
Paul Baumann ◽  
Linda Baumann
Keyword(s):  
Liquid Medium ◽  
Type Strain ◽  
Solid Medium ◽  
Morphological Characterization ◽  
The Other ◽  
Polar Flagellum ◽  
Phenotypic Characterization ◽  
Phenotypic Traits ◽  
General Properties

Eighty-six strains which were isolated from cases of gastroenteritis and had the general properties of the genus Beneckea were submitted to an extensive nutritional, physiological, and morphological characterization. The results indicated that this collection of strains, which included the type strain of Beneckea parahaemolytica, was phenotypically homogeneous and distinguishable from the other known species of Beneckea by multiple, unrelated, phenotypic traits. When grown in liquid medium, strains of B. parahaemolytica had single, sheathed, polar flagella; when grown on solid medium, these strains had unsheathed, peritrichous flagella in addition to the sheathed, polar flagellum. Additional traits of use for differentiation of this species from the remaining species of the genus Beneckea were the ability of B. parahaemolytica to grow at 40 C, utilize d-galactose, l-leucine, l-histidine, and putrescine and the inability to utilize sucrose, dl-β-hydroxy-butyrate or give a positive Voges-Proskauer reaction. The validity of some of the traits previously used to identify B. parahaemolytica as well as the possible difficulties encountered in the identification of this organism from marine sources are considered.

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