scholarly journals Association of sequences in the coat protein/readthrough domain of potato mop-top virus with transmission by Spongospora subterranea.

1998 ◽  
Vol 79 (10) ◽  
pp. 2343-2347 ◽  
Author(s):  
B Reavy ◽  
G H Cowan ◽  
M Arif ◽  
L Torrance
Keyword(s):  
Coat Protein ◽  
Spongospora Subterranea ◽  
Potato Mop Top Virus

scholarly journals Initial infection of roots and leaves reveals different resistance phenotypes associated with coat protein gene-mediated resistance to Potato mop-top virus

2002 ◽  
Vol 83 (5) ◽  
pp. 1201-1209 ◽  
Author(s):  
Anna Germundsson ◽  
Maria Sandgren ◽  
Hugh Barker ◽  
Eugene I. Savenkov ◽  
Jari P. T. Valkonen
Keyword(s):  
Coat Protein ◽  
Nicotiana Benthamiana ◽  
Protein Gene ◽  
Resistance Mechanism ◽  
Spongospora Subterranea ◽  
Mechanical Inoculation ◽  
Systemic Movement ◽  
Cp Gene ◽  
Transgenic Potatoes ◽  
Potato Mop Top Virus

Resistance to the pomovirus Potato mop-top virus (PMTV) was studied in potato (Solanum tuberosum cv. Saturna) and Nicotiana benthamiana transformed with the coat protein (CP) gene of PMTV. The incidence of PMTV infections was reduced in tubers of the CP-transgenic potatoes grown in the field in soil infested with the viruliferous vector, Spongospora subterranea. However, in those tubers that were infected, all three virus RNAs were detected and virus titres were high. The CP-transgenic N. benthamiana plants were inoculated with PMTV using two methods. Following mechanical inoculation of leaves, no RNA 3 (the CP-encoding RNA homologous to the transgene) was detected in leaves, but in some plants low amounts of RNA 3 were detected in roots; RNA 2 was readily detected in leaves and roots of several plants. Inoculation of roots using viruliferous S. subterranea resulted in infection of roots in all plants and the three PMTV RNAs were detected. However, no systemic movement of PMTV from roots to the above-ground parts was observed, indicating a novel expression of resistance. These data indicate that the CP gene-mediated resistance to PMTV specifically restricts accumulation of PMTV RNA 3, and is more effective in leaves than roots. Furthermore, expression of resistance is different depending on whether leaves or roots are inoculated. Data do not exclude the possibility that both a protein-mediated and an RNA-mediated resistance mechanism are involved.

scholarly journals The beet virus Q coat protein readthrough domain is longer than previously reported, with two transmembrane domains

2009 ◽  
Vol 90 (3) ◽  
pp. 754-758 ◽  
Author(s):  
François Crutzen ◽  
Marguerite Kreit ◽  
Claude Bragard
Keyword(s):  
Amino Acid ◽  
Coat Protein ◽  
Chenopodium Quinoa ◽  
Transmembrane Domains ◽  
Terminal Part ◽  
Consensus Amino Acid Sequence ◽  
The Common ◽  
Potato Mop Top Virus ◽  
Beet Virus Q ◽  
Consensus Amino Acid

Ten beet virus Q (BVQ) strains from six different countries were sequenced to characterize the readthrough (RT) domain of the coat protein (CP). The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are FM244643–FM244652. With three nucleotide additions of 5, 285 and 1 nt, the common RT of 76 kDa was found to be longer than the single reference available to date (35 kDa). It is hypothesized that multiple inoculation cycles on Chenopodium quinoa were responsible for these three deletions in the C-terminal part of the BVQ RNA-2 previously described. Two putative transmembrane domains, TM1 and TM2, were predicted in the consensus amino acid sequence of the ten BVQ strains, and the putative BVQ TM2 was aligned with that of potato mop-top virus.

Production of recombinant Potato mop-top virus coat protein in Escherichia coli and generation of antisera recognising native virus protein

2003 ◽  
Vol 110 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Valérie Hélias ◽  
Emmanuel Jacquot ◽  
Maryse Guillet ◽  
Yves Le Hingrat ◽  
Danièle Giblot-Ducray
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Virus Protein ◽  
Virus Coat Protein ◽  
Native Virus ◽  
Potato Mop Top Virus ◽  
Virus Coat

scholarly journals Potato mop-top virus: the coat protein-encoding RNA and the gene for cysteine-rich protein are dispensable for systemic virus movement in Nicotiana benthamiana

2003 ◽  
Vol 84 (4) ◽  
pp. 1001-1005 ◽  
Author(s):  
Eugene I. Savenkov ◽  
Anna Germundsson ◽  
Andrey A. Zamyatnin ◽  
Maria Sandgren ◽  
Jari P. T. Valkonen
Keyword(s):  
Coat Protein ◽  
Nicotiana Benthamiana ◽  
Virus Movement ◽  
Cysteine Rich Protein ◽  
Protein Encoding ◽  
Potato Mop Top Virus

scholarly journals A REVIEW PAPER ON POTATO MOP-TOP VIRUS (PMTV): OCCURRENCE, PROPERTIES AND MANAGEMENT

2016 ◽  
Vol 1 (3) ◽  
pp. 129 ◽  
Author(s):  
Aqleem Abbas ◽  
Meysam Madadi
Keyword(s):  
Coat Protein ◽  
Management Strategies ◽  
Triple Gene Block ◽  
Economic Losses ◽  
Tuber Quality ◽  
Potato Tubers ◽  
Long Distance ◽  
Gene Block ◽  
Potato Mop Top Virus ◽  
Important Disease

Potato mop-top virus (PMTV) is a plant pathogenic virus that affects potatoes. The virus was initially reported from Germany but now it has spread throughout Europe, Asia, South America and North America. It is responsible for spraing symptoms (brown arcs/lines, blemishes, and rings) on potato tubers and yellow chevrons or mopping (Shortened internodes) in the leaves and stems of plants grown from infected potato tubers. PMTV causes huge economic losses due to poor tuber quality. It is an important disease in the potato growing areas of the world. PMTV is tubular rod shape and has a single stranded positive sense RNA (+ssRNA) tripartite genome. RNA 1 encodes RdRp (viral RNA-dependent RNA polymerase). Coat protein (20kDa) and a larger protein (91kDa) is encoded by RNA2. RNA2 encodes larger protein (91 kDa) by read through (RT) of the amber termination codon of the coat protein. There are three conserved moldular sets of genes known as triple gene block (TGB) which are coded by RNA3. These TGBs are involved in cell to cell or long distance movement of PMTV. In nature, PMTV is vectored and transmitted by a soil born pathogen (Plasmodiophorid (Spongospora subterranean f.sp. subterranean abbreviated as ‘Sss’) that itself causes the powdery scab disease on tubers. The disease caused by PMTV and Sss are favored by cool and damp conditions. PMTV remain in spore balls of Sss for several years even if the potato is not grown in the field. There are no efficient means to manage the virus nor its vector in an infested field, therefore, preventive measures are essential. Since PMTV along with its vector is causing important disease of potato, so understanding its molecular, biological, physical properties and management strategies is very important.

scholarly journals Perunan maltokaariviruksen leviäminen perunakasvin eri osiin

2008 ◽  
pp. 1-6
Author(s):  
Johanna Aura ◽  
Jari P.T. Valkonen
Keyword(s):  
Spongospora Subterranea ◽  
Potato Mop Top Virus

Perunan maltokaarivirus (Potato mop-top virus, PMTV) on Pohjoismaissa laajalle levinnyt ja vaikeastitorjuttava maalevintäinen taudinaiheuttaja. Sen levittäjänä pellolla toimii sienenkaltainen mikrobi,Spongospora subterranea, joka aiheuttaa mukuloiden pintaan kuorirokkoa. PMTV aiheuttaamukuloihin kuoliota, maltokaarioireita, ja tuottaa siten laatutappioita.PMTV tartuttaa mukuloita ja perunan juuristoa, mutta se havaitaan harvoin maanpäällisissäosissa kuten lehdissä. Siten PMTV-tartunnan havaitseminen perunakasvustossa kasvukauden aikanatai testaaminen idätettyjen mukuloiden versoista ei ole mahdollista samaan tapaan kuin muidenperunanvirusten osalta. Havaintojemme mukaan PMTV kuitenkin leviää mukulasta ituihin varastooloissa(5ºC, pimeä). Tämä viittaa siihen, että virus kykenisi sinänsä leviämään kehittyviin versoihin,mutta leviäminen estyy vielä tuntemattoman mekanismin vaikutuksesta. Tutkimuksemme tavoitteenaoli selvittää tätä mekanismia.Kasvatushuonekokeissa PMTV:n havaittiin leviävän infektoituneesta siemenmukulasta kaikkiinperunan maanalaisiin osiin: juuriin, maavarsiin sekä niihin muodostuviin mukuloihin. Lehdissä virustaoli vain hyvin vähäisiä määriä, jotka olivat niin pieniä, että ne voitiin havaita vain testaamallavirusgenomin (RNA) läsnäoloa kvantitatiivisen PCR-menetelmän avulla, mutta ei virustestaukseennormaalisti käytettävän, vasta-aineisiin perustuvan ELISA-menetelmän avulla. Itävien mukuloidenaltistaminen valolle ja korkeammalle lämpötilalle johti virusmäärien vähentymiseen iduissa. Näidentulosten pohjalta tarkastellaan lähemmin virus-RNA:han kohdentuvaa hajotusta, ns. RNA-hiljennystä,mahdollisena mekanismina, joka estää PMTV:n kerääntymistä perunan yhteyttäviin osiin.

Development of High Resolution DNA Melting Analysis for Simultaneous Detection of Potato Mop-Top Virus and Its Vector, Spongospora subterranea, in Soil

Plant Disease ◽  
2020 ◽  
Author(s):  
Xianzhou Nie ◽  
Mathuresh Singh ◽  
Dahu Chen ◽  
Cassandra Gilchrist ◽  
Yasmine Soqrat ◽  
...  
Keyword(s):  
High Resolution ◽  
Simultaneous Detection ◽  
Soil Samples ◽  
Dna Melting ◽  
Spongospora Subterranea ◽  
Rt Pcr ◽  
Efficient Detection ◽  
Melting Analysis ◽  
Potato Mop Top Virus ◽  
Negative Detection

In this study, a set of duplex reverse transcription (RT)-PCR-mediated high resolution DNA melting (HRM) analyses for simultaneous detection of potato mop-virus (PMTV) and its protist vector, Spongospora subterranea f.sp. subterranea (Sss), was developed. The infestation of soil by PMTV was detected by using a tobacco-based baiting system. Total RNA extracted from the soil led to successful RT-PCR gel-electrophoresis detection of both PMTV and Sss. To facilitate more efficient detection, newly designed primer pairs for PMTV RNA species (i.e., RNA-Rep, -CP, and -TGB) were analyzed together with the existing Sss primers using real-time RT-PCR. The resulting amplicons exhibited melting profiles that could be readily differentiated. Under duplex RT-PCR format, all PMTV and Sss primer combinations led to successful detection of respective PMTV RNA species and Sss in the samples by high resolution DNA melting (HRM) analyses. When the duplex HRM assay was applied to soil samples collected from six fields at four different sites in New Brunswick, Canada, positive detection of PMTV and/or Sss was found in 63-100% samples collected from fields in which PMTV-infected tubers had been observed. In contrast, the samples from fields where neither PMTV- nor Sss-infected tubers had been observed resulted in negative detection by the assay. Bait tobacco bioassay for PMTV and Sss produced similar results. Between 63%-83% and 100% of the soil samples collected from PMTV-infested fields led to PMTV and Sss infections in the bait tobacco plants, respectively; whereas no PMTV or Sss infected plants were obtained from soil samples collected from PMTV/Sss-free fields.

scholarly journals Detection and Confirmation of Potato mop-top virus in Potatoes Produced in the United States and Canada

Plant Disease ◽  
2004 ◽  
Vol 88 (4) ◽  
pp. 363-367 ◽  
Author(s):  
H. Xu ◽  
T.-L. DeHaan ◽  
S. H. De Boer
Keyword(s):  
United States ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
The United States ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
The North ◽  
Potato Mop Top Virus ◽  
Pcr Targeting

Potato mop-top virus (PMTV) was detected in potatoes grown in the United States and Canada during surveillance testing by a reverse transcription-polymerase chain reaction (RT-PCR) targeting the coat protein gene in RNA3. Out of 3,221 lots of seed and ware potatoes that were tested, 4.3% were positive for PMTV. The reliability of the survey results was confirmed by reextraction of selected samples and additional RT-PCR tests using two primer sets targeting gene segments in RNA2 and RNA3. Amplicons generated from RNA2 and RNA3 were identified by analysis of fragment length polymorphisms after digestion with BamHI and HindIII, respectively. PMTV was further identified by enzyme-linked immunosorbent assay, bioassay on Nicotiana debneyi, and transmission electron microscopy. Sequencing of a portion of the coat protein gene revealed near 100% identity among isolates from the United States and Canada and >97% homology of the North American isolates with European isolates.

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