scholarly journals Genotype H: a new Amerindian genotype of hepatitis B virus revealed in Central America

2002 ◽  
Vol 83 (8) ◽  
pp. 2059-2073 ◽  
Author(s):  
Patricia Arauz-Ruiz ◽  
Helene Norder ◽  
Betty H. Robertson ◽  
Lars O. Magnius
Keyword(s):  
Hepatitis B Virus ◽  
Los Angeles ◽  
Hepatitis B ◽  
Central America ◽  
Amino Acid Residues ◽  
Hbv Genotype ◽  
Separate Branch ◽  
B Virus ◽  
Complete Genomes ◽  
Genotype F

The complete genomes were sequenced for ten hepatitis B virus (HBV) strains. Two of them, from Spain and Sweden, were most similar to genotype D, although encoding d specificity. Five of them were from Central America and belonged to genotype F. Two strains from Nicaragua and one from Los Angeles, USA, showed divergences of 3·1–4·1% within the small S gene from genotype F strains and were recognized previously as a divergent clade within genotype F. The complete genomes of the two genotype D strains were found to differ from published genotype D strains by 2·8–4·6%. Their S genes encoded Lys122, Thr127 and Lys160, corresponding to the putative new subtype adw3 within this genotype, previously known to specify ayw2, ayw3 or, rarely, ayw4. The complete genomes of the three divergent strains diverged by 0·8–2·5% from each other, 7·2–10·2% from genotype F strains and 13·2–15·7% from other HBV strains. Since pairwise comparisons of 82 complete HBV genomes of intratypic and intertypic divergences ranged from 0·1 to 7·4% and 6·8 to 17·1%, respectively, the three sequenced strains should represent a new HBV genotype, for which the designation H is proposed. In the polymerase region, the three strains had 16 unique conserved amino acid residues not present in genotype F strains. So far, genotype H has been encountered in Nicaragua, Mexico and California. Phylogenetic analysis of the complete genomes and subgenomes of the three strains showed them clustering with genotype F but forming a separate branch supported by 100% bootstrap. Being most similar to genotype F, known to be an Amerindian genotype, genotype H has most likely split off from genotype F within the New World.

scholarly journals Molecular Evolution of Hepatitis B Virus Genotype F in Latin America

2021 ◽  
Author(s):  
Jonas Wolf ◽  
Thiago Kastell Mazeto ◽  
Vagner Reinaldo Zingalli Bueno Pereira ◽  
Daniel Simon ◽  
Vagner Ricardo Lunge
Keyword(s):  
Latin America ◽  
Hepatitis B Virus ◽  
Molecular Evolution ◽  
Hepatitis B ◽  
Recent Common Ancestor ◽  
Virus Genotype ◽  
Hbv Genotype ◽  
Most Recent Common Ancestor ◽  
B Virus ◽  
Genotype F

Abstract Hepatitis B virus (HBV) genotype F evolution is not completely understood in Latin America. This study aims to evaluate the molecular evolution of HBV-F in Latin America by comparing 224 whole-genome sequences. Bayesian coalescent analysis was performed to estimate the time to the most recent common ancestor. Four main clades were formed dated back between 1245 and 1730. Also, four subclades were identified dated back between 1705 and 1801. HBV-F overall effective population size grew in the 18th century and showed an initial circulation of HBV-F from Venezuela to other countries from Latin America.

scholarly journals Characterization of genotype H hepatitis B virus strain identified for the first time from a Japanese blood donor by nucleic acid amplification test

2005 ◽  
Vol 86 (3) ◽  
pp. 595-599 ◽  
Author(s):  
Hitoshi Ohnuma ◽  
Akira Yoshikawa ◽  
Hideaki Mizoguchi ◽  
Hiroaki Okamoto ◽  
Keyword(s):  
Hepatitis B Virus ◽  
Nucleic Acid ◽  
Los Angeles ◽  
Hepatitis B ◽  
Early Stage ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Hbv Genotype ◽  
First Case ◽  
B Virus

The Japanese Red Cross has been conducting a nucleic acid amplification test (NAT) screening for hepatitis B virus (HBV), hepatitis C virus and human immunodeficiency virus 1 among blood donors since July 1 1999. The first case of HBV genotype H was found and reported in Japan. Serological markers of HBV were not detected in this NAT-positive donation. It may be that the positive donation was in the serological window period at the early stage of infection. The complete genome of 3215 nt was sequenced, and the sequence had 99·3 % homology with the strain from Los Angeles, USA (LSA2523). Here, a leucine zipper motif was found in the region of the HBV surface antigen conserved through genotypes A–H.

Phylogenetic analysis of the hepatitis B virus (HBV) genotype F including Argentine isolates

2001 ◽  
Vol 146 (9) ◽  
pp. 1803-1810 ◽  
Author(s):  
V. A. Mbayed ◽  
L. Barbini ◽  
J. L. López ◽  
R. H. Campos
Keyword(s):  
Phylogenetic Analysis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hbv Genotype ◽  
B Virus ◽  
Genotype F

scholarly journals Antigenic Diversity of Hepatitis B Virus Strains of Genotype F in Amerindians and Other Population Groups from Venezuela

1998 ◽  
Vol 36 (3) ◽  
pp. 648-651 ◽  
Author(s):  
Linda Blitz ◽  
Flor H. Pujol ◽  
Paul D. Swenson ◽  
Leticia Porto ◽  
Ricardo Atencio ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hepatitis B Surface Antigen ◽  
Serum Samples ◽  
Hbv Genotype ◽  
Hbsag Carriers ◽  
Hbsag Subtype ◽  
B Virus ◽  
Virus Strains ◽  
Genotype F

The adw4 subtype of hepatitis B virus (HBV) belongs to a unique genomic group (genotype F) representing the original HBV strains from the New World. Data regarding the prevalence of this subtype among HBV carriers in South America are, however, scarce, and those concerning HBV genotype F are based on only a few samples from Latin America. In this study, serum samples were obtained from 141 hepatitis B surface antigen (HBsAg) carriers from Amerindians and urban populations from Venezuela. The HBsAg subtype was identified with monoclonal antibodies in 105 samples, and the HBV genotype was identified by reverse-phase hybridization with DNA fragments in 58 samples. The adw4 subtype was highly prevalent in the population studied (75%); among the Amerindians, the prevalence was 97%. The adw2 subtype was also present (10%), while other subtypes (ayw3 and ayw4) were only occasionally found. The HBV subtype was associated with the expected genotype in most cases (80%), and thus genotype F was highly prevalent. Sequencing of viral strains that gave genotypes unpredicted by the HBsAg subtyping confirmed seven of them as belonging to not previously described genotype-subtype associations: namely, adw2 and ayw4 within genotype F.

scholarly journals Characterization of hepatitis B virus genotypes among Yucpa Indians in Venezuela

2001 ◽  
Vol 82 (2) ◽  
pp. 359-365 ◽  
Author(s):  
Tatsunori Nakano ◽  
Ling Lu ◽  
Xiaolei Hu ◽  
Masashi Mizokami ◽  
Etsuro Orito ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Complete Genome ◽  
Deletion Mutants ◽  
Reading Frame ◽  
B Virus ◽  
Virus Genotypes ◽  
Core Reading ◽  
Genotype F

The complete genome sequences of hepatitis B virus (HBV) from 12 HBV-infected Yucpa Indians of Venezuela, a group with highly endemic HBV, were amplified and sequenced. The 12 isolates were closely related to each other, with 98·6–100% nucleotide identity. A phylogenetic tree based on the complete genome indicated clearly that they were genotype F. Three individuals had evidence of infection with two different HBV deletion mutants. In two individuals, a three amino acid deletion was identified just prior to the ‘a’ determinant loop of the S region. A third individual was infected with virus that contained a complete core reading frame and a population that contained a deletion in the middle of the core region. These results indicate that genotype F HBV is present in the Venezuelan Yucpa Amerindians and the complete genome sequence allowed the identification of two unique deletion mutants in a limited set of samples.

A case of hepatitis B virus infection in Eritrean Diciotti migrant: phylogenetic analysis and ‘mirror effect’

2019 ◽  
Vol 14 (8) ◽  
pp. 509-514
Author(s):  
Giancarlo Ceccarelli ◽  
Eleonora Cella ◽  
Serena Vita ◽  
Alessia Lai ◽  
Erika Ebranati ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Copy Number ◽  
Mirror Effect ◽  
Hepatitis B Virus Infection ◽  
Migrant Population ◽  
Hbv Genotype ◽  
B Virus ◽  
Epidemiological Approach

A case of hepatitis B virus (HBV) infection in an Eritrean migrant was described to provide an epidemiological approach based on phylogenetic analysis useful in developing countries with lacking information. Migrant, positive for HBsAg and HBeAg, carried HBV at high copy number. A sequence of HBV HBsAg region was used for phylogenetic relationships and genetic variability investigation. In the phylogenetic tree, the sequence corresponded to D2 HBV genotype and the cluster root dated 7 years ago. These data compared with the date of landing in Italy, suggest that he was infected at least 7 years before his arrival. This approach by ‘mirror effect’ allows the reconstruction of HBV epidemiology in the country of origin, analyzing the migrant population in the host country.

Dynamics of a hepatitis B virus e antigen minus population ascribed to genotype F during the course of a chronic infection despite the presence of anti-HBs antibodies

2007 ◽  
Vol 123 (1) ◽  
pp. 72-85 ◽  
Author(s):  
V.L. Mathet ◽  
J.L. López ◽  
V. Ruiz ◽  
D.O. Sánchez ◽  
G. Carballal ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Infection ◽  
B Virus ◽  
Genotype F
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