scholarly journals Marinobacter halophilus sp. nov., a halophilic bacterium isolated from a salt lake

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2838-2845 ◽  
Author(s):  
Zhi-Ping Zhong ◽  
Ying Liu ◽  
Hong-Can Liu ◽  
Fang Wang ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Trees ◽  
Salt Lake ◽  
Qaidam Basin ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone

A Gram-staining-negative bacterium, strain XCD-X12T, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9 %, w/w) in Qaidam basin, Qinghai Province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X12T were non-spore-forming rods, 0.4–0.7 μm wide, 2.1–3.2 μm long and motile with a single polar flagellum. Strain XCD-X12T was strictly aerobic and catalase- and oxidase-positive. Growth was observed in the presence of 0–20.0 % (w/v) NaCl (optimum, 4.0–8.0 %), at 4–35 °C (optimum, 30 °C) and at pH 6.5–10.5 (optimum, pH 8.5). It contained Q-9 as the predominant respiratory quinone. The major fatty acids (>10.0 %) were C16 : 0, C16 : 1ω9c and C18 : 1ω9c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and an uncharacterized aminophospholipid. The DNA G+C content was 55.6 mol% (T m). Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X12T was associated with the genus Marinobacter, and showed the highest 16S rRNA gene sequence similarity to Marinobacter hydrocarbonoclasticus ATCC 49840T (97.4 %), M. vinifirmus FB1T (96.8 %), M. excellens KMM 3809T (96.8 %) and M. antarcticus ZS2-30T (96.7 %). DNA–DNA relatedness of strain XCD-X12T to M. hydrocarbonoclasticus CGMCC 1.7683T was 34 ± 5 %. Based on these data, it is concluded that strain XCD-X12T represents a novel species of the genus Marinobacter, for which the name Marinobacter halophilus sp. nov. is proposed. The type strain is XCD-X12T ( = CGMCC 1.12481T = JCM 30472T).

scholarly journals Maritimibacter lacisalsi sp. nov., isolated from a salt lake, and emended description of the genus Maritimibacter Lee et al. 2007

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3462-3468 ◽  
Author(s):  
Zhi-Ping Zhong ◽  
Ying Liu ◽  
Yu-Guang Zhou ◽  
Hong-Can Liu ◽  
Fang Wang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Trees ◽  
Novel Species ◽  
Salt Lake ◽  
Qaidam Basin ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Respiratory Quinone

A Gram-staining-negative, non-motile, strictly aerobic bacterium, strain X12M-4T, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9 %, w/w) in Qaidam basin, Qinghai Province, China. Its taxonomic position was determined by using a polyphasic approach. Strain X12M-4T was catalase- and oxidase-positive. Cells were rod-shaped, 0.5–0.8 μm wide and 1.1–1.6 μm long. Growth was observed in the presence of 0–11.0 % (w/v) NaCl (optimum, 5.0–6.0 %) and at 15–40 °C (optimum, 25 °C) and pH 6.5–9.5 (optimum, pH 7.0). No growth occurred at 10 °C or 45 °C. Strain X12M-4T contained C18 : 1ω7c, C19 : 0 cyclo ω8c and C16 : 0 as the major fatty acids (>10.0 %). The predominant respiratory quinone was Q-10.The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidglycerol, an unknown aminolipid and an unidentified lipid. The DNA G+C content was 65.7 mol% (determined using T m). Strain X12M-4T showed highest 16S rRNA gene sequence similarities to Maritimibacter alkaliphilus HTCC2654T (96.7 %), Roseibacterium elongatum DSM 19469T (96.4 %), Tropicimonas aquimaris DPG-21T (95.6 %), ‘Roseibacterium beibuensis’ JLT1202r (95.6 %) and Tropicimonas sediminicola M97T (95.5 %) and < 95.5 % to others. Phylogenetic trees based on 16S rRNA gene sequences indicated that strain X12M-4T formed a robust cluster with M. alkaliphilus HTCC2654T. On the basis of the data, it is concluded that strain X12M-4T represents a novel species of the genus Maritimibacter, for which the name Maritimibacter lacisalsi sp. nov. is proposed. The type strain is X12M-4T ( = CGMCC 1.12922T = JCM 30555T). To accommodate the novel species, the description of the genus Maritimibacter was emended.

scholarly journals Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China

2006 ◽  
Vol 56 (2) ◽  
pp. 373-377 ◽  
Author(s):  
Jee-Min Lim ◽  
Che Ok Jeon ◽  
Sang-Mi Lee ◽  
Jae-Chan Lee ◽  
Li-Hua Xu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Moderately Halophilic Bacterium ◽  
Bacterium Strain

A moderately halophilic bacterium, strain BH169T, capable of growing at salinities of 3–20 % (w/v) NaCl was isolated from a saline lake in China. Strain BH169T was strictly aerobic, short-rod-shaped and non-motile (non-flagellated). Its major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C16 : 0. The genomic DNA G+C content was about 43 mol% and the predominant quinone was MK-7. The cell-wall peptidoglycan was of the A1γ type, containing meso-diaminopimelic acid as the diagnostic diamino acid. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a distinct phylogenetic line within the spore-forming rods of the genus Bacillus. The levels of 16S rRNA gene sequence similarity to the type strains of Bacillus species were below 93 %. On the basis of phenotypic and molecular properties, strain BH169T (=KCTC 3912T=DSM 16461T) represents the type strain of a novel species within the genus Bacillus, for which the name Bacillus salarius sp. nov. is proposed.

scholarly journals Pseudomonas salina sp. nov., isolated from a salt lake

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2846-2851 ◽  
Author(s):  
Zhi-Ping Zhong ◽  
Ying Liu ◽  
Ting-Ting Hou ◽  
Hong-Can Liu ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Trees ◽  
Salt Lake ◽  
Qaidam Basin ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Respiratory Quinone ◽  
Qinghai Province ◽  
Gram Staining

A Gram-staining-negative, facultatively aerobic bacterium, strain XCD-X85T, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9  %, w/v) in Qaidam basin, Qinghai province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X85T were non-endospore-forming rods, 0.4–0.6 μm wide and 1.0–1.6 μm long, and motile by means of a single polar flagellum. Strain XCD-X85T was catalase- and oxidase-positive. Growth was observed in the presence of 0–12.0  % (w/v) NaCl (optimum, 1.0–2.0  %) and at 4–35 °C (optimum, 25–30 °C) and pH 6.5–10.5 (optimum, pH 8.0–8.5). Strain XCD-X85T contained (>10  %) summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C12 : 0, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the predominant fatty acids. The major respiratory quinone was ubiquinone 9 (Q-9). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 57.4 mol%. Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X85T was associated with the genus Pseudomonas, and showed highest 16S rRNA gene sequence similarities to Pseudomonas pelagia CL-AP6T (99.0  %) and Pseudomonas bauzanensis BZ93T (96.8  %). DNA–DNA relatedness of strain XCD-X85T to P. pelagia JCM 15562T was 19 ± 1  %. On the basis of the data presented above, it is concluded that strain XCD-X85T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas salina sp. nov. is proposed. The type strain is XCD-X85T ( = CGMCC 1.12482T = JCM 19469T).

Psychroserpens damuponensis sp. nov., isolated from seawater

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 703-708 ◽  
Author(s):  
Dong-Heon Lee ◽  
Sun Ja Cho ◽  
Suk Min Kim ◽  
Sun Bok Lee
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Unidentified Phospholipid ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type

A novel bacterium, designated strain F051-1T, isolated from a seawater sample collected from the coast at Damupo beach in Pohang, Korea, was investigated in a polyphasic taxonomic study. Cells were yellow-pigmented, strictly aerobic, Gram-staining-negative and rod-shaped. The temperature, pH and NaCl ranges for growth were 4–30 °C, pH 6.0–9.0 and 1.0–6.0 % (w/v), respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain F051-1T belongs to the genus Psychroserpens in the family Flavobacteriaceae . Its closest relatives were Psychroserpens burtonensis ACAM 188T (96.8 % 16S rRNA gene sequence similarity) and Psychroserpens mesophilus KOPRI 13649T (95.7 %). The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G and anteiso-C15 : 0. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, two unidentified aminolipids, one unidentified phospholipid and eight unidentified lipids. The major respiratory quinone was menaquinone-6 and the genomic DNA G+C content of the strain was 33.5 mol%. On the basis of phenotypic, phylogenetic and genotypic data, strain F051-1T represents a novel species within the genus Psychroserpens , for which the name Psychroserpens damuponensis sp. nov. is proposed. The type strain is F051-1T ( = KCTC 23539T  = JCM 17632T).

scholarly journals Hyunsoonleella jejuensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seawater

2010 ◽  
Vol 60 (2) ◽  
pp. 382-386 ◽  
Author(s):  
Byoung-Jun Yoon ◽  
Dong-Heon Lee ◽  
Bong-Jo Kang ◽  
Hyung-Yeel Kahng ◽  
You-Sung Oh ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Carotenoid Pigment ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
The Family ◽  
Genotypic Characteristics

A novel marine, Gram-staining-negative, yellow-pigmented, rod-shaped bacterial strain, designated CNU004T, was isolated from a seawater sample collected on the coastline of Jeju Island, South Korea. The strain was strictly aerobic, non-flagellated, non-gliding and oxidase- and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CNU004T belongs to a distinct lineage in the family Flavobacteriaceae. Strain CNU004T exhibited levels of 16S rRNA gene sequence similarity of 93.8–93.9 % to its nearest phylogenetic neighbours, members of the genera Gaetbulibacter, Yeosuana and Algibacter. The new isolate required sea salts or artificial seawater for growth. The optimum ranges of temperature and pH for growth were 30–35 °C and pH 7.0–8.0. The DNA G+C content of strain CNU004T was 37.7 mol%. The major fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and iso-C15 : 0 3-OH. Menaquinone-6 was the major respiratory quinone. Zeaxanthin was the major carotenoid pigment produced, and flexirubin-type pigments were not produced. Strain CNU004T was able to degrade starch and agar. Based on its phenotypic and genotypic characteristics and on the phylogenetic evidence presented, strain CNU004T is considered to represent a novel species of a new genus in the family Flavobacteriaceae, for which the name Hyunsoonleella jejuensis gen. nov., sp. nov. is proposed. The type strain of Hyunsoonleella jejuensis sp. nov. is CNU004T (=KCTC 22242T =DSM 21035T).

scholarly journals Halovenus salina sp. nov., an extremely halophilic archaeon isolated from a saltern

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 3016-3023 ◽  
Author(s):  
Carmen Infante-Domínguez ◽  
Paulina Corral ◽  
Cristina Sánchez-Porro ◽  
Antonio Ventosa
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Minor Amount ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Halophilic Archaeon ◽  
Respiratory Quinone ◽  
Extremely Halophilic Archaeon ◽  
A Minor

An extremely halophilic archaeon was isolated from a water sample of Isla Bacuta saltern in Huelva, Spain. Strain ASP54T is a novel red-pigmented, motile, rod-shaped, Gram-stain-negative and strictly aerobic haloarchaeon. Strain ASP54T grew in media containing 15–30  % (w/v) salts and optimally with 25  % (w/v) salts. It grew between pH 5.0 and 9.0 (optimally at pH 7.5) and at 20–40 °C (optimally at 37 °C). Phylogenetic analysis based on multi-locus sequence analysis (MLSA) and the comparison of 16S rRNA gene sequences revealed that strain ASP54T is most closely related to the genus Halovenus. The closest relatives were Halovenus aranensis EB27T (92.1  % 16S rRNA gene sequence similarity), Halorientalis regularis TNN28T (92.1  %), and Halorientalis persicus D108T (92.0  %). The polar lipid pattern of strain ASP54T consisted of biphosphatidylglycerol, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, sulfated mannosyl glucosyl diether and a minor-phospholipid. The predominant respiratory quinone was menaquinone-8 (MK-8) (83  %), and a minor amount of MK-8(VIII-H2) (17  %) was also detected. The G+C content of the genomic DNA of this strain was 63.1 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain ASP54T represents a novel species of the genus Halovenus, for which the name Halovenus salina sp. nov. is proposed. The type strain is ASP54T ( = CECT 8749T = IBRC-M 10946T = JCM 30072T).

scholarly journals Luteimonas soli sp. nov., isolated from farmland soil

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4809-4815 ◽  
Author(s):  
Xiang Wang ◽  
Hong-Xing Yang ◽  
Ying-Kun Zhang ◽  
Shi-Jun Zhu ◽  
Xiao-Wei Liu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Farmland Soil ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene

A yellow-pigmented bacterial strain, designated Y2T, was isolated from farmland soil in Bengbu, Anhui province, China. Cells of strain Y2T were Gram-stain-negative, strictly aerobic, non-motile and rod-shaped. Strain Y2T grew optimally at pH 7.0, 30 °C and in the presence of 2 % (w/v) NaCl. The DNA G+C content was 68.9 mol%. The major fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 0, summed feature 9 (C16 : 0 10-methyl and/or iso-C17 : 1ω9c), iso-C11 : 0 3-OH and iso-C11 : 0. The major respiratory quinone was ubiquinone-8 (Q-8), and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain Y2T was most closely related to Luteimonas mephitis B1953/27.1T (99.1 % 16S rRNA gene sequence similarity), followed by Luteimonas lutimaris G3T (98.6 %), Luteimonas abyssi XH031T (96.2 %) and Luteimonas aquatica RIB1-20T (96.0 %). Strain Y2T exhibited low DNA–DNA relatedness with Luteimonas mephitis B1953/27.1T (43.6 ± 0.5 %) and Luteimonas lutimaris G3T (43.9 ± 2.1 %). On the basis of phenotypic, genotypic and phylogenetic evidence, strain Y2T represents a novel species of the genus Luteimonas, for which the name Luteimonas soli sp. nov. is proposed. The type strain is Y2T ( = ACCC 19799T = KCTC 42441T).

scholarly journals Jiella aquimaris gen. nov., sp. nov., isolated from offshore surface seawater

2015 ◽  
Vol 65 (Pt_4) ◽  
pp. 1127-1132 ◽  
Author(s):  
Jing Liang ◽  
Ji Liu ◽  
Xiao-Hua Zhang
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Surface Seawater ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type

A Gram-stain-negative, strictly aerobic and rod-shaped motile bacterium with peritrichous flagella, designated strain LZB041T, was isolated from offshore surface seawater of the East China Sea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LZB041T formed a lineage within the family ‘ Aurantimonadaceae ’ that was distinct from the most closely related genera Aurantimonas (96.0–96.4 % 16S rRNA gene sequence similarity) and Aureimonas (94.5–96.0 %). Optimal growth occurred in the presence of 1–7 % (w/v) NaCl, at pH 7.0–8.0 and at 28–37 °C. Ubiquinone-10 was the predominant respiratory quinone. The major fatty acids (>10 % of total fatty acids) were C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8) and cyclo-C19 : 0ω8c. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, one unknown aminolipid, one unknown phospholipid and one unknown polar lipid. The DNA G+C content of strain LZB041T was 71.3 mol%. On the basis of polyphasic analysis, strain LZB041T is considered to represent a novel species of a new genus in the class Alphaproteobacteria , for which the name Jiella aquimaris gen. nov., sp. nov. is proposed. The type strain of the type species is LZB041T ( = JCM 30119T = MCCC 1K00255T).

Aliidiomarina indica sp. nov., isolated from deep seawater

2021 ◽  
Vol 71 (12) ◽  
Author(s):  
Lei Song ◽  
Hongcan Liu ◽  
Ying Huang ◽  
Xin Dai ◽  
Yuguang Zhou
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Phylogenetic Trees ◽  
Sea Water ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type

A Gram-stain-negative, rod-shaped bacterial strain, designated SW123T, was isolated from a deep-sea water sample collected from the Indian Ocean. Strain SW123T was strictly aerobic, catalase- and oxidase-positive. The predominant cellular fatty acids were iso-C15 : 0, iso-C17 : 0 and summed feature 9 (comprising C16 : 0-methyl or iso-C17 : 1 ω9c). Ubiquinone-8 was the sole respiratory quinone. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content was 49.4 mol%. 16S rRNA gene sequence analysis showed that strain SW123T was closely related to Aliidiomarina shirensis AIST (96.7 % sequence similarity), Aliidiomarina iranensis GBPy7T (96.3%), Aliidiomarina haloalkalitolerans AK5T (96.0%) and Aliidiomarina celeris F3105T (95.9%). Phylogenetic trees based on 16S rRNA gene sequences showed that strain SW123T represented a novel member of the genus Aliidiomarina , forming a distinct cluster with A. celeris F3105T. On the basis of phylogenetic inference and phenotypic characteristics, we propose that strain SW123T represents a novel species of the genus Aliidiomarina , with the name Aliidiomarina indica sp. nov. The type strain is SW123T (=CGMCC1.16169T=KCTC 82234T).

Leucothrix pacifica sp. nov., isolated from seawater, and emended description of the genus Leucothrix

2015 ◽  
Vol 65 (Pt_8) ◽  
pp. 2397-2402 ◽  
Author(s):  
Zenghu Zhang ◽  
Xin Gao ◽  
Long Wang ◽  
Xiao-Hua Zhang
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Ocean Drilling Program ◽  
Respiratory Quinone ◽  
Integrated Ocean Drilling Program ◽  
South Pacific Gyre

A Gram-stain-negative, strictly aerobic, non-flagellated, non-gliding, oxidase- and catalase-positive, white-pigmented and rod-shaped bacterium, designated strain XH122T, was isolated from a surface seawater sample collected from the South Pacific Gyre (45° 58′ E 163° 11′ S) during Integrated Ocean Drilling Program Expedition 329.Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XH122T belonged to the genus Leucothrix and showed highest 16S rRNA gene sequence similarity to Leucothrix mucor DSM 2157T (94.3 %). It showed lower sequence similarities ( < 90.7 %) with all other representatives of the class Gammaproteobacteria. Optimal growth occurred in the presence of 2 % (w/v) NaCl, at pH 8.0 and at 28 °C. The DNA G+C content of strain XH122T was 46.2 mol%. The major fatty acids were C16 : 0, C16 : 1ω9c and C18 : 1ω9c. The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. On the basis of data from this polyphasic study, strain XH122T is considered to represent a novel species of the genus Leucothrix, for which the name Leucothrix pacifica sp. nov. is proposed. The type strain is XH122T ( = DSM 25984T = JCM 18388T).

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