scholarly journals Bacteroides paurosaccharolyticus sp. nov., isolated from a methanogenic reactor treating waste from cattle farms

2011 ◽  
Vol 61 (2) ◽  
pp. 448-453 ◽  
Author(s):  
Atsuko Ueki ◽  
Kunihiro Abe ◽  
Yoshimi Ohtaki ◽  
Nobuo Kaku ◽  
Kazuya Watanabe ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
Major Respiratory Quinone ◽  
Cattle Farms ◽  
Type Strains

A strictly anaerobic bacterial strain (WK042T) was isolated from rice-straw residue in a methanogenic reactor treating waste from cattle farms in Japan. Cells were Gram-staining-negative, non-motile, non-spore-forming rods. Growth was stimulated well by haemin, and was enhanced by cobalamin (vitamin B12). Strain WK042T utilized arabinose, xylose, glucose, mannose and aesculin as preferred substrates. Maltose, dextrin, glycogen, starch and pectin were also utilized, although growth on these substrates was much slower. The strain produced acetate, propionate and succinate from these saccharides. The strain was slightly alkaliphilic, with optimum growth at pH 7.7. The temperature range for growth was 10–40 °C, the optimum being 35 °C. The strain was sensitive to bile. The major cellular fatty acids were anteiso-C15 : 0, iso-C17 : 0 3-OH and C15 : 0. Menaquinone 11 (MK-11) was the major respiratory quinone and the genomic DNA G+C content was 41.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed the strain in the phylum Bacteroidetes. Strain WK042T was related distantly to the type strains of species in the cluster including Bacteroides massiliensis, Bacteroides vulgatus and Bacteroides dorei (91–92 % 16S rRNA gene sequence similarity). Based on data from the present phylogenetic, physiological and chemotaxonomic analyses, strain WK042T is considered to represent a novel species of the genus Bacteroides, for which the name Bacteroides paurosaccharolyticus sp. nov. is proposed. The type strain is WK042T (=JCM 15092T =DSM 21004T).

scholarly journals Bizionia arctica sp. nov., isolated from Arctic fjord seawater, and emended description of the genus Bizionia

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2925-2930 ◽  
Author(s):  
Hai Li ◽  
Xi-Ying Zhang ◽  
Chang Liu ◽  
Ang Liu ◽  
Qi-Long Qin ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
Arctic Fjord ◽  
Polyphasic Characterization ◽  
Type Strains

A Gram-stain-negative, yellow-pigmented, aerobic, non-flagellated, non-gliding bacterial strain, designated SM1203T, was isolated from surface seawater of Kongsfjorden, Svalbard. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM1203T was affiliated with the genus Bizionia in the family Flavobacteriaceae. The strain shared the highest 16S rRNA gene sequence similarity (>96  %) with the type strains of Formosa spongicola (96.8  %), Bizionia paragorgiae (96.3  %), B. saleffrena (96.3  %) and B. echini (96.1  %) and 95.4–95.7  % sequence similarity with the type strains of other known species of the genus Bizionia. The strain grew at 4–30 °C and in the presence of 1.0–5.0  % (w/v) NaCl. The major fatty acids of strain SM1203T were iso-C15  :  0, iso-C15  :  1, anteiso-C15  :  0 and C15  :  0 and the main polar lipids were phosphatidylethanolamine and an unidentified lipid. The major respiratory quinone of strain SM1203T was menaquinone 6 (MK-6). The genomic DNA G+C content of strain SM1203T was 34.8 mol%. Based on the polyphasic characterization of strain SM1203T in this study, the strain represents a novel species in the genus Bizionia, for which the name Bizionia arctica sp. nov. is proposed. The type strain is SM1203T ( = CGMCC 1.12751T = JCM 30333T). An emended description of the genus Bizionia is also given.

scholarly journals Paracoccus stylophorae sp. nov., isolated from the reef-building coral Stylophora pistillata

2011 ◽  
Vol 61 (9) ◽  
pp. 2221-2226 ◽  
Author(s):  
Shih-Yi Sheu ◽  
Sing-Rong Jiang ◽  
Chaolun Allen Chen ◽  
Jih-Terng Wang ◽  
Wen-Ming Chen
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Stylophora Pistillata ◽  
Major Respiratory Quinone ◽  
Type Strains

A bacterial strain, designated KTW-16T, was isolated from the reef-building coral Stylophora pistillata, collected from southern Taiwan. Strain KTW-16T was a Gram-negative, facultatively anaerobic, pale-yellow, non-motile short rod. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KTW-16T belonged to the genus Paracoccus in the Alphaproteobacteria and exhibited 93.7–96.9 % 16S rRNA gene sequence similarity with type strains of species of the genus Paracoccus (96.9 % with Paracoccus alcaliphilus JCM 7364T). Strain KTW-16T grew at 15–40 °C (optimum 35 °C), at pH 6.0–10.0 (optimum pH 8.0) and with 0–9 % NaCl (optimum 5 %). The predominant cellular fatty acids were C18 : 1ω7c, C19 : 0 cyclo ω8c and C18 : 0. The major respiratory quinone was Q-10 and the DNA G+C content was 69.1 mol%. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and several unknown polar lipids. The physiological and biochemical tests allowed clear phenotypic differentiation of the isolate from the type strains of already described Paracoccus species. It is evident from the genotypic, phenotypic and chemotaxonomic analysis that strain KTW-16T should be classified in a novel species of the genus Paracoccus, for which the name Paracoccus stylophorae sp. nov. is proposed. The type strain is KTW-16T ( = LMG 25392T  = BCRC 80106T).

scholarly journals Hymenobacter arcticus sp. nov., isolated from glacial till

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 2113-2118 ◽  
Author(s):  
Xulu Chang ◽  
Jingli Zheng ◽  
Fan Jiang ◽  
Ping Liu ◽  
Wenjing Kan ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Svalbard Archipelago ◽  
Content Type ◽  
Link Type ◽  
Major Respiratory Quinone ◽  
Type Strains

A novel, red–pink-pigmented strain, designated R2-4T, was isolated from a till sample near Ny-Alesund, Svalbard Archipelago, Norway. Cells were aerobic, Gram-stain-negative and rod-shaped. Growth occurred at 4–30 °C (optimum, 20–22 °C), at pH 6.0–9.0 (optimum, pH 7.0) and with 0–1 % NaCl added to R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R2-4T belonged to the genus Hymenobacter . 16S rRNA gene sequence similarity between strain R2-4T and the type strains of related species of the genus ranged from 94.51 to 96.05 %. Strain R2-4T contained iso-C15 : 0, anteiso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), summed feature 4 (C17 : 1 anteiso B and/or iso I) and C16 : 1ω5c as the major cellular fatty acids, MK-7 as the major respiratory quinone, and phosphatidylethanolamine, unknown aminophospholipids, unknown aminolipids and unknown lipids as the main polar lipids. The polyamine was sym-homospermidine. The DNA G+C content of strain R2-4T was 61.6 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain R2-4T is considered to represent a novel species of the genus Hymenobacter , for which the name Hymenobacter arcticus sp. nov. is proposed. The type strain is R2-4T ( = CCTCC AB 2012104T = KACC 16881T).

scholarly journals Marinobacter gudaonensis sp. nov., isolated from an oil-polluted saline soil in a Chinese oilfield

2007 ◽  
Vol 57 (2) ◽  
pp. 250-254 ◽  
Author(s):  
Jun Gu ◽  
Hua Cai ◽  
Su-Lin Yu ◽  
Ri Qu ◽  
Bin Yin ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Saline Soil ◽  
Sequence Similarity ◽  
Eastern China ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Shengli Oilfield ◽  
Major Respiratory Quinone ◽  
Reference Strains

Two novel strains, SL014B61AT and SL014B11A, were isolated from an oil-polluted saline soil from Gudao in the coastal Shengli Oilfield, eastern China. Cells of strains SL014B61AT and SL014B11A were motile, Gram-negative and rod-shaped. Growth occurred at NaCl concentrations of between 0 and 15 % and at temperatures of between 10 and 45 °C. Strain SL014B61AT had Q9 as the major respiratory quinone and C16 : 0 (21.2 %), C18 : 1ω9c (20.3 %), C16 : 1ω7c (7.3 %) and C16 : 1ω9c (6.4 %) as predominant fatty acids. The G+C content of the DNA was 57.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SL014B61AT belonged to the genus Marinobacter in the class Gammaproteobacteria. Strain SL014B61AT showed the highest 16S rRNA gene sequence similarity with Marinobacter bryozoorum (97.9 %) and showed 97.8 % sequence similarity to Marinobacter lipolyticus. DNA–DNA relatedness to the reference strains Marinobacter bryozoorum and Marinobacter lipolyticus was 35.5 % and 33.8 %, respectively. On the basis of these data, it is proposed that strains SL014B61AT and SL014B11A represent a novel species, Marinobacter gudaonensis sp. nov. The type strain is strain SL014B61AT (=DSM 18066T=LMG 23509T=CGMCC 1.6294T).

scholarly journals Hyunsoonleella jejuensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seawater

2010 ◽  
Vol 60 (2) ◽  
pp. 382-386 ◽  
Author(s):  
Byoung-Jun Yoon ◽  
Dong-Heon Lee ◽  
Bong-Jo Kang ◽  
Hyung-Yeel Kahng ◽  
You-Sung Oh ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Carotenoid Pigment ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
The Family ◽  
Genotypic Characteristics

A novel marine, Gram-staining-negative, yellow-pigmented, rod-shaped bacterial strain, designated CNU004T, was isolated from a seawater sample collected on the coastline of Jeju Island, South Korea. The strain was strictly aerobic, non-flagellated, non-gliding and oxidase- and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CNU004T belongs to a distinct lineage in the family Flavobacteriaceae. Strain CNU004T exhibited levels of 16S rRNA gene sequence similarity of 93.8–93.9 % to its nearest phylogenetic neighbours, members of the genera Gaetbulibacter, Yeosuana and Algibacter. The new isolate required sea salts or artificial seawater for growth. The optimum ranges of temperature and pH for growth were 30–35 °C and pH 7.0–8.0. The DNA G+C content of strain CNU004T was 37.7 mol%. The major fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and iso-C15 : 0 3-OH. Menaquinone-6 was the major respiratory quinone. Zeaxanthin was the major carotenoid pigment produced, and flexirubin-type pigments were not produced. Strain CNU004T was able to degrade starch and agar. Based on its phenotypic and genotypic characteristics and on the phylogenetic evidence presented, strain CNU004T is considered to represent a novel species of a new genus in the family Flavobacteriaceae, for which the name Hyunsoonleella jejuensis gen. nov., sp. nov. is proposed. The type strain of Hyunsoonleella jejuensis sp. nov. is CNU004T (=KCTC 22242T =DSM 21035T).

scholarly journals Luteimonas soli sp. nov., isolated from farmland soil

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4809-4815 ◽  
Author(s):  
Xiang Wang ◽  
Hong-Xing Yang ◽  
Ying-Kun Zhang ◽  
Shi-Jun Zhu ◽  
Xiao-Wei Liu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Farmland Soil ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene

A yellow-pigmented bacterial strain, designated Y2T, was isolated from farmland soil in Bengbu, Anhui province, China. Cells of strain Y2T were Gram-stain-negative, strictly aerobic, non-motile and rod-shaped. Strain Y2T grew optimally at pH 7.0, 30 °C and in the presence of 2 % (w/v) NaCl. The DNA G+C content was 68.9 mol%. The major fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 0, summed feature 9 (C16 : 0 10-methyl and/or iso-C17 : 1ω9c), iso-C11 : 0 3-OH and iso-C11 : 0. The major respiratory quinone was ubiquinone-8 (Q-8), and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain Y2T was most closely related to Luteimonas mephitis B1953/27.1T (99.1 % 16S rRNA gene sequence similarity), followed by Luteimonas lutimaris G3T (98.6 %), Luteimonas abyssi XH031T (96.2 %) and Luteimonas aquatica RIB1-20T (96.0 %). Strain Y2T exhibited low DNA–DNA relatedness with Luteimonas mephitis B1953/27.1T (43.6 ± 0.5 %) and Luteimonas lutimaris G3T (43.9 ± 2.1 %). On the basis of phenotypic, genotypic and phylogenetic evidence, strain Y2T represents a novel species of the genus Luteimonas, for which the name Luteimonas soli sp. nov. is proposed. The type strain is Y2T ( = ACCC 19799T = KCTC 42441T).

scholarly journals Ochrobactrum ciceri sp. nov., isolated from nodules of Cicer arietinum

2010 ◽  
Vol 60 (7) ◽  
pp. 1548-1553 ◽  
Author(s):  
A. Imran ◽  
F. Y. Hafeez ◽  
A. Frühling ◽  
P. Schumann ◽  
K. A. Malik ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Cicer Arietinum ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gram Staining ◽  
Type Strains

A Gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, Ca-34T, was isolated from nodules of chickpea (Cicer arietinum) in Pakistan and studied for its taxonomic affiliation. The almost full-length 16S rRNA gene sequence showed highest similarities to those of strains of the genus Ochrobactrum. Based on results of MALDI-TOF MS and 16S rRNA gene sequence similarity (98.6 %), strain Ca-34T and Ochrobactrum intermedium LMG 3301T are phylogenetic neighbours; the two strains shared DNA–DNA relatedness of 64 %. The fatty acid profile [predominantly C18 : 1 ω7c (67.7 %) and C19 : 0 cyclo ω8c (19.6 %)] also supported the genus affiliation. Metabolically, strain Ca-34T differed from other type strains of Ochrobactrum in many reactions and from all type strains in testing positive for gelatin hydrolysis and in testing negative for assimilation of alaninamide and l-threonine. Based on phenotypic and genotypic data, we conclude that strain Ca-34T represents a novel species, for which we propose the name Ochrobactrum ciceri sp. nov. (type strain Ca-34T =DSM 22292T =CCUG 57879T).

scholarly journals Formosa spongicola sp. nov., isolated from the marine sponge Hymeniacidon flavia

2011 ◽  
Vol 61 (2) ◽  
pp. 330-333 ◽  
Author(s):  
Byoung-Jun Yoon ◽  
Duck-Chul Oh
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Marine Sponge ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
The Family ◽  
Major Respiratory Quinone

A Gram-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated, oxidase- and catalase-positive, marine bacterium, designated A2T, was isolated from a marine sponge, Hymeniacidon flavia, collected from the coast of Jeju Island, South Korea. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that strain A2T was a member of the family Flavobacteriaceae. Its closest relatives were Formosa agariphila KMM 3901T and Formosa algae KMM 3553T (96.99 and 96.98 % 16S rRNA gene sequence similarity, respectively). DNA–DNA relatedness between strain A2T and F. agariphila KMM 3901T and F. algae KMM 3553T was 14.1 and 26.8 %, respectively. The dominant fatty acids (>5 %) of strain A2T were iso-C15 : 0 (33.9 %), iso-C17 : 0 3-OH (20.8 %), iso-C15 : 1 G (10.5 %) and iso-C15 : 0 3-OH (6.1 %). The DNA G+C content of strain A2T was 36.0 mol% and the major respiratory quinone was MK-6. On the basis of phenotypic and phylogenetic analysis, strain A2T represents a novel species of the genus Formosa, for which the name Formosa spongicola sp. nov. is proposed. The type strain is A2T (=KCTC 22662T =DSM 22637T).

Photobacterium aquae sp. nov., isolated from a recirculating mariculture system

2014 ◽  
Vol 64 (Pt_2) ◽  
pp. 475-480 ◽  
Author(s):  
Ying Liu ◽  
Liang-Zi Liu ◽  
Lei Song ◽  
Yu-Guang Zhou ◽  
Fang-Jun Qi ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Optimum Ph ◽  
Distinct Phylogenetic Lineage

A Gram-staining-negative, heterotrophic, facultatively anaerobic bacterium, designated AE6T, was isolated from a grouper (Epinephelus malabaricas) culture tank in a recirculating mariculture system located in Tianjin, China. Strain AE6T was able to grow at 15–40 °C (optimum, 30–35 °C), at pH 5.5–10.0 (optimum, pH 7.0–7.5) and in the presence of 0.5–7 % (w/v) NaCl (optimum, 2–3 %). It contained Q-8 as the predominant respiratory quinone, phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) as the major polar lipids and C16 : 1ω7c/C16 : 1ω6c (40.4 %), C18 : 1ω7c (15.5 %) and C16 : 0 (13.5 %) as the predominant cellular fatty acids. The genomic DNA G+C content was 47.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AE6T belonged to the genus Photobacterium (94.2–96.8 % of 16S rRNA gene sequence similarity) and formed a distinct phylogenetic lineage within the genus and exhibited the highest sequence similarity to Photobacterium aphoticum CECT 7614T (96.8 %). Multilocus sequence analysis (MLSA) using four loci (gyrB, rpoA, pyrH and recA) also revealed that strain AE6T was phylogenetically related to the genus Photobacterium . Based on the phylogenetic, chemotaxonomic and phenotypic evidence, strain AE6T is considered to represent a novel species of the genus Photobacterium , for which the name Photobacterium aquae sp. nov. is proposed. The type strain is AE6T ( = CGMCC 1.12159T = JCM 18480T).

scholarly journals Marinobacter koreensis sp. nov., isolated from sea sand in Korea

2006 ◽  
Vol 56 (11) ◽  
pp. 2653-2656 ◽  
Author(s):  
Byung-Yong Kim ◽  
Hang-Yeon Weon ◽  
Seung-Hee Yoo ◽  
Jong-Shik Kim ◽  
Soon-Wo Kwon ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Sea Sand ◽  
Moderately Halophilic Bacterium ◽  
Genotypic Characteristics ◽  
Type Strains

A marine, Gram-negative, aerobic, motile, straight-rod-shaped, moderately halophilic bacterium, designated strain DD-M3T, was isolated from sea sand in Pohang, Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that the strain fell within the evolutionary radiation encompassed by the genus Marinobacter. The levels of 16S rRNA gene sequence similarity between the novel strain and the type strains of recognized Marinobacter species ranged from 94.2 to 97.6 %, the highest values being with Marinobacter flavimaris SW-145T (97.6 %) and Marinobacter lipolyticus SM19T (96.8 %). The values for DNA–DNA relatedness between isolate DD-M3T and the type strains of the most closely related species, M. flavimaris and M. lipolyticus, were 41 and 36 %, respectively. Strain DD-M3T was characterized as having Q-9 as the predominant respiratory quinone and 16 : 0, summed feature 3 and 18 : 1ω9c as the main fatty acids. The DNA G+C content was 54.1 mol%. On the basis of its phenotypic and genotypic characteristics, DD-M3T represents a novel species of the genus Marinobacter, for which the name Marinobacter koreensis sp. nov. is proposed, with DD-M3T (=KACC 11513T=DSM 17924T) as the type strain.

Sign in / Sign up

Export Citation Format

Share Document