scholarly journals Paenibacillus favisporus sp. nov., a xylanolytic bacterium isolated from cow faeces

2004 ◽  
Vol 54 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Encarna Velázquez ◽  
Trinidad de Miguel ◽  
Margarita Poza ◽  
Raúl Rivas ◽  
Ramón Rosselló-Mora ◽  
...  
Keyword(s):  
Novel Species ◽  
Hydrolytic Enzymes ◽  
Gas Production ◽  
Cow Dung ◽  
Rrna Gene ◽  
The Novel ◽  
Closely Related Species ◽  
Main Fatty Acid ◽  
Phenylalanine Deaminase ◽  
Micro Organisms

During a search for xylan-degrading micro-organisms, a sporulated bacterium was recovered from recent and old cow dung and rectal samples. The isolates were identified as members of a novel species of the genus Paenibacillus, based on 16S rRNA gene sequences. According to the results of phylogenetic analysis, the most closely related species was Paenibacillus azoreducens. Phenotypic and chemotaxonomic analyses and DNA–DNA hybridization experiments also showed that the isolates belonged to a novel species of the genus Paenibacillus. The novel species is a facultatively anaerobic, motile, Gram-variable, sporulated rod. The spores of this rod-shaped micro-organism occur in slightly swollen sporangia and are honeycomb-shaped. The main fatty acid is anteiso-branched C15 : 0. Growth was observed with many carbohydrates, including xylan, as the only carbon source and gas production was not observed from glucose. The novel species produces a wide variety of hydrolytic enzymes, such as xylanases, cellulases, amylases, gelatinase, urease and β-galactosidase. On the contrary, it does not produce caseinase, phenylalanine deaminase or lysine decarboxylase. According to the data obtained in this work, the strains belong to a novel species, for which the name Paenibacillus favisporus sp. nov. is proposed (type strain, GMP01T=LMG 20987T=CECT 5760T).

scholarly journals Wickerhamiella allomyrinae f.a., sp. nov., a yeast species isolated from the gut of the rhinoceros beetle Allomyrina dichotoma

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3856-3861 ◽  
Author(s):  
Yong-Cheng Ren ◽  
Yun Wang ◽  
Liang Chen ◽  
Tao Ke ◽  
Feng-Li Hui
Keyword(s):  
Related Species ◽  
Nature Reserve ◽  
Yeast Species ◽  
Novel Species ◽  
Sequence Divergence ◽  
Rrna Gene ◽  
The Novel ◽  
Closely Related Species ◽  
Rhinoceros Beetle ◽  
Lsu Rrna Gene

Two strains representing Wickerhamiella allomyrinae f.a., sp. nov. were isolated from the gut of Allomyrina dichotoma (Coleoptera: Scarabeidae) collected from the Baotianman National Nature Reserve, Nanyan, Henan Province, China. Sequence analyses of the D1/D2 domains of the LSU rRNA gene revealed that this novel species was located in the Wickerhamiella clade (Saccharomycetes, Saccharomycetales), with three described species of the genus Candida, namely Candida musiphila, Candida spandovensis and Candida sergipensis, as the most closely related species. The novel species differed from these three species by 9.3–9.8 % sequence divergence (35–45 nt substitutions) in the D1/D2 sequences. The species could also be distinguished from the closely related species, C. musiphila, C. spandovensis and C. sergipensis, by growth on vitamin-free medium and at 37 °C. The type strain is Wickerhamiella allomyrinae sp. nov. NYNU 13920T ( = CICC 33031T = CBS 13167T).

scholarly journals Hanseniaspora jakobsenii sp. nov., a yeast isolated from Bandji, a traditional palm wine of Borassus akeassii

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3576-3579 ◽  
Author(s):  
Labia Irène I. Ouoba ◽  
Dennis S. Nielsen ◽  
Amarachukwu Anyogu ◽  
Christine Kando ◽  
Bréhima Diawara ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Its Region ◽  
Rrna Gene ◽  
No Production ◽  
The Novel ◽  
Closely Related Species ◽  
Palm Wine ◽  
26S Rrna ◽  
Genotypic Characteristics

Investigation of the microbial diversity of Bandji, a traditional palm wine from Burkina Faso (West Africa) revealed the presence of two yeast isolates (YAV16 and YAV17T) with unusual phenotypic and genotypic characteristics. The isolates divide by bipolar budding with no production of ascospores. Phylogenetic analysis of concatenated sequences of the 26S rRNA gene D1/D2 and internal transcribed spacer (ITS) regions indicated that the novel species was most closely related to Kloeckera lindneri and Hanseniaspora valbyensis. The new isolates differed from K. lindneri NRRL Y-17531T and H. valbyensis CBS 479T by substitutions in the D1/D2 region of 12 and 16 nt respectively. The divergence in the ITS region from the closely related species was characterized by substitutions of 45–46 nt. Repetitive palindromic PCR (rep-PCR) profiles of YAV16 and YAV17T were also significantly different from those of K. lindneri MUCL 31146T ( = NRRL Y-17531T), H. valbyensis NCYC 17T ( = CBS 479T) and other species of the genus Hanseniaspora. Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new isolates represent a novel species for which the name Hanseniaspora jakobsenii sp. nov. is proposed with YAV17T ( = CBS 12942T = DSM 26339T = NCYC 3828T; MycoBank number MB 805785) as the type strain.

scholarly journals Cryptococcus agrionensis sp. nov., a basidiomycetous yeast of the acidic rock drainage ecoclade, isolated from an acidic aquatic environment of volcanic origin

2010 ◽  
Vol 60 (4) ◽  
pp. 996-1000 ◽  
Author(s):  
Gabriel Russo ◽  
Diego Libkind ◽  
Ricardo J. Ulloa ◽  
Virginia de García ◽  
Jose P. Sampaio ◽  
...  
Keyword(s):  
Aquatic Environment ◽  
Novel Species ◽  
Large Subunit ◽  
Physiological Data ◽  
Rrna Gene ◽  
The Novel ◽  
Closely Related Species ◽  
Volcanic Origin ◽  
Acidic Rock ◽  
Large Subunit Rrna

Seventy-one strains were isolated from the River Agrio–Lake Caviahue acidic aquatic environment in Argentina. Strains were isolated mainly from the most acidic section of the river (pH 1.8–2.7). According to the mini/microsatellite-primed PCR technique and physiological data, these strains are representatives of a single novel species of the genus Cryptococcus. Analysis of the D1/D2 region of the large-subunit rRNA gene showed that the strains belong to the order Filobasidiales of the subphylum Agaricomycotina. The novel isolates formed a phylogenetic group with Cryptococcus ibericus, Cryptococcus aciditolerans and Cryptococcus metallitolerans, the most closely related species. This group, which is phylogenetically related to the Gastricus clade, is considered to be an ecoclade due to its peculiar ecology and physiology. The name Cryptococcus agrionensis sp. nov. is proposed to accommodate these isolates, with strain CRUB 1317T (=CBS 10799T=JCM 15321T) as the type strain.

scholarly journals Paenibacillus xylanilyticus sp. nov., an airborne xylanolytic bacterium

2005 ◽  
Vol 55 (1) ◽  
pp. 405-408 ◽  
Author(s):  
Raúl Rivas ◽  
Pedro F. Mateos ◽  
Eustoquio Martínez-Molina ◽  
Encarna Velázquez
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
Sequence Similarity ◽  
Hydrolytic Enzymes ◽  
16S Rrna Gene Sequencing ◽  
Gas Production ◽  
Rrna Gene ◽  
Hybridization Data ◽  
Paenibacillus Illinoisensis ◽  
Rrna Gene Sequencing

During a search for xylan-degrading micro-organisms, a sporulating bacterium was recovered from xylan-containing agar plates exposed to air in a research laboratory (Salamanca University, Spain). The airborne isolate (designated strain XIL14T) was identified by 16S rRNA gene sequencing as representing a Paenibacillus species most closely related to Paenibacillus illinoisensis JCM 9907T (99·3 % sequence similarity) and Paenibacillus pabuli DSM 3036T (98 % sequence similarity). Phenotypic, chemotaxonomic and DNA–DNA hybridization data indicated that the isolate belongs to a novel species of the genus Paenibacillus. Cells of strain XIL14T were motile, sporulating, rod-shaped, Gram-positive and facultatively anaerobic. The predominant cellular fatty acids were anteiso-C15 : 0 and C16 : 0. The DNA G+C content of strain XIL14T was 50·5 mol%. Growth was observed with many carbohydrates, including xylan, as the only carbon source and gas production was not observed from glucose. Catalase was positive and oxidase was negative. The airborne isolate produced a variety of hydrolytic enzymes, including xylanases, amylases, gelatinase and β-galactosidase. DNA–DNA hybridization levels between strain XIL14T and P. illinoisensis DSM 11733T and P. pabuli DSM 3036T were 43·3 and 36·3 %, respectively. According to the data obtained, strain XIL14T is considered to represent a novel species for which the name Paenibacillus xylanilyticus sp. nov. is proposed (=LMG 21957T=CECT 5839T).

scholarly journals Sympodiomyces attinorum sp. nov., a yeast species associated with nests of the leaf-cutting ant Atta sexdens

2004 ◽  
Vol 54 (5) ◽  
pp. 1891-1894 ◽  
Author(s):  
Solange C. Carreiro ◽  
Fernando C. Pagnocca ◽  
Maurício Bacci ◽  
Marc-André Lachance ◽  
Odair C. Bueno ◽  
...  
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Growth Characteristics ◽  
Rrna Gene ◽  
The Novel ◽  
Atta Sexdens ◽  
Leaf Cutting ◽  
Large Subunit Rrna ◽  
Waste Deposit

Four strains of a novel yeast species were isolated from laboratory nests of the leaf-cutting ant Atta sexdens in Brazil. Three strains were found in older sponges and one was in a waste deposit in the ant nests. Sequencing of the D1/D2 region of the large-subunit rRNA gene showed that the novel species, named Sympodiomyces attinorum sp. nov., is phylogenetically related to Sympodiomyces parvus. Unlike Sympodiomyces parvus, Sympodiomyces attinorum can ferment glucose, assimilate methyl α-d-glucoside, salicin and citrate, and grow at 37 °C, thus enabling these two species to be distinguished. Differentiation from other related species is possible on the basis of other growth characteristics. The type strain of Sympodiomyces attinorum is UNESP-S156T (=CBS 9734T=NRRL Y-27639T).

scholarly journals Mycobacterium europaeum sp. nov., a scotochromogenic species related to the Mycobacterium simiae complex

2011 ◽  
Vol 61 (7) ◽  
pp. 1606-1611 ◽  
Author(s):  
Enrico Tortoli ◽  
Erik C. Böttger ◽  
Anna Fabio ◽  
Enevold Falsen ◽  
Zoe Gitti ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Polyphasic Analysis ◽  
Mycobacterium Simiae

Four strains isolated in the last 15 years were revealed to be identical in their 16S rRNA gene sequences to MCRO19, the sequence of which was deposited in GenBank in 1995. In a polyphasic analysis including phenotypic and genotypic features, the five strains (including MCRO19), which had been isolated in four European countries, turned out to represent a unique taxonomic entity. They are scotochromogenic slow growers and are genetically related to the group that included Mycobacterium simiae and 15 other species. The novel species Mycobacterium europaeum sp. nov. is proposed to accommodate these five strains. Strain FI-95228T ( = DSM 45397T  = CCUG 58464T) was chosen as the type strain. In addition, a thorough revision of the phenotypic and genotypic characters of the species related to M. simiae was conducted which leads us to suggest the denomination of the ‘Mycobacterium simiae complex’ for this group.

scholarly journals Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil

2010 ◽  
Vol 60 (1) ◽  
pp. 244-248 ◽  
Author(s):  
Melissa Fontes Landell ◽  
Raisa Billodre ◽  
Jesus P. Ramos ◽  
Orílio Leoncini ◽  
Marilene H. Vainstein ◽  
...  
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Sequence Divergence ◽  
Rrna Gene ◽  
The Novel ◽  
D2 Domain ◽  
Its Gene ◽  
Ascomycetous Yeasts ◽  
Positive Results

Two novel yeast species, Candida aechmeae sp. nov. and Candida vrieseae sp. nov., were isolated from bromeliads in Itapuã Park, Rio Grande do Sul, Brazil. These species are genetically isolated from all other currently recognized ascomycetous yeasts based on their sequence divergence in the D1/D2 domain of the LSU rRNA gene. C. aechmeae sp. nov. is phylogenetically close to Candida ubatubensis, a species also isolated from bromeliads in Brazil, but the novel species can be differentiated on the basis of differences in the D1/D2 domain and positive results for the assimilation of l-arabinose, raffinose, inulin and citrate. Candida vrieseae sp. nov. is phylogenetically placed in a clade near Candida membranifaciens that is composed of several species associated with insects, but the novel species can be differentiated from them by the D1/D2 and ITS gene sequences, positive results for the assimilation of nitrite and a negative result for the assimilation of ethylamine. The type strain for Candida aechmeae sp. nov. is BI153T (=CBS 10831T=NRRL Y-48456T) and the type strain for C. vrieseae sp. nov. is BI146T (=CBS 10829T=NRRL Y-48461T).

scholarly journals Ogataea mangiferae sp. nov., a methylotrophic yeast isolated from mango leaves

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1855-1859 ◽  
Author(s):  
Ana Raquel O. Santos ◽  
Elisa S. Faria ◽  
Marc-André Lachance ◽  
Carlos A. Rosa
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Mangifera Indica ◽  
Large Subunit ◽  
Its Region ◽  
Methylotrophic Yeast ◽  
Rrna Gene ◽  
The Novel ◽  
Mango Leaves

Five strains of a novel methanol-assimilating yeast species were isolated from mango (Mangifera indica) leaves collected at the campus of the Federal University of Minas Gerais in Brazil. The sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit of the rRNA gene showed that this species belongs to the Ogataea clade and is related to O. allantospora, O. chonburiensis, O. dorogensis, O. kodamae, O. paradorogensis and Candida xyloterini (Ogataea clade). The novel species differs in the D1/D2 domains of the large subunit of the rRNA gene by 12 to 40 substitutions from these Ogataea species. The name Ogataea mangiferae sp. nov. is proposed for this novel species. The type strain of Ogataea mangiferae sp. nov. is UFMG-CM-Y253T ( = CBS 13492T). The Mycobank number is MB 811646.

scholarly journals Altererythrobacter namhicola sp. nov. and Altererythrobacter aestuarii sp. nov., isolated from seawater

2011 ◽  
Vol 61 (4) ◽  
pp. 709-715 ◽  
Author(s):  
Seong Chan Park ◽  
Keun Sik Baik ◽  
Han Na Choe ◽  
Chae Hong Lim ◽  
Ho Jun Kim ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
The Family ◽  
South Sea ◽  
Ubiquinone 10 ◽  
Pigmented Bacteria

Two non-motile, orange- or yellow-pigmented bacteria, designated strains KYW48T and KYW147T, were isolated from seawater collected from the South Sea, Republic of Korea. Cells of both strains were Gram-reaction-negative, aerobic and catalase- and oxidase-positive. The major fatty acids of strain KYW48T were C18 : 1ω7c (35.3 %), summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) (22.7 %), C17 : 1ω6c (19.8 %), C14 : 0 2-OH (7.4 %) and C16 : 0 (5.9 %), and those of strain KYW147T were C18 : 1ω7c (36.0 %), summed feature 3 (18.3 %), C16 : 0 (14.7 %), 11-methyl C18 : 1ω7c (10.7 %), C16 : 0 2-OH (9.1 %) and C18 : 1ω9c (8.0 %). The predominant isoprenoid quinone of both strains was ubiquinone 10 (Q-10). The DNA G+C contents of strains KYW48T and KYW147T were 63.8 and 67.2 mol%, respectively. A phylogenetic tree based on 16S rRNA gene sequences showed that strains KYW48T and KYW147T were grouped with the members of the family Erythrobacteraceae and formed a distinct clade with the members of the genus Altererythrobacter (<95.7 % sequence similarity). On the basis of the evidence presented in this study, the novel species Altererythrobacter namhicola sp. nov. (type strain KYW48T  = KCTC 22736T  = JCM 16345T) and Altererythrobacter aestuarii sp. nov. (type strain KYW147T  = KCTC 22735T  = JCM 16339T) are proposed.

Microbulbifer chitinilyticus sp. nov. and Microbulbifer okinawensis sp. nov., chitin-degrading bacteria isolated from mangrove forests

2011 ◽  
Vol 61 (9) ◽  
pp. 2215-2220 ◽  
Author(s):  
Atsushi Baba ◽  
Masayuki Miyazaki ◽  
Takahiko Nagahama ◽  
Yuichi Nogi
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Threshold Level ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Mangrove Forests ◽  
The Novel ◽  
Separate Species ◽  
Gene Sequence Analysis ◽  
Degrading Bacteria

Three chitin-degrading strains representing two novel species were isolated from mangrove forests in Okinawa, Japan. The isolates, ABABA23T, ABABA211 and ABABA212T, were Gram-negative, non-spore-forming, strictly aerobic chemo-organotrophs. The novel strains produced Q-8 as the major isoprenoid quinone component. The predominant fatty acids were iso-C15 : 0 and C16 : 0. On the basis of 16S rRNA gene sequence analysis, the isolates were closely affiliated with members of the genus Microbulbifer. The DNA G+C contents of strains ABABA23T and ABABA212T were 57.8 and 60.2 mol%, respectively. DNA–DNA relatedness values between these two strains and Microbulbifer reference strains were significantly lower than 70 %, the generally accepted threshold level below which strains are considered to belong to separate species. Based on differences in taxonomic characteristics, the three isolates represent two novel species of the genus Microbulbifer, for which the names Microbulbifer chitinilyticus sp. nov. (type strain, ABABA212T = JCM 16148T = NCIMB 14577T) and Microbulbifer okinawensis sp. nov. (type strain, ABABA23T = JCM 16147T = NCIMB 14576T; reference strain, ABABA211) are proposed.

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