scholarly journals Cellulomonas bogoriensis sp. nov., an alkaliphilic cellulomonad

2005 ◽  
Vol 55 (4) ◽  
pp. 1711-1714 ◽  
Author(s):  
Brian E. Jones ◽  
William D. Grant ◽  
A. W. Duckworth ◽  
Peter Schumann ◽  
Norbert Weiss ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Optimal Growth ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Diamino Acid ◽  
Bacterium Strain ◽  
Metabolic Properties ◽  
Type Strains ◽  
The 16S Rrna Gene

An alkaliphilic, slightly halotolerant, chemo-organotrophic, Gram-positive, rod-shaped bacterium, strain 69B4T, was isolated from the sediment of the littoral zone of Lake Bogoria, Kenya. Phylogenetically, it is a member of the genus Cellulomonas, showing less than 97·5 % sequence similarity to the type strains of other Cellulomonas species. The highest level of similarity, albeit moderate, was found with respect to Cellulomonas cellasea DSM 20118T. Chemotaxonomic properties confirm the 16S rRNA gene-based generic affiliation, i.e. a DNA G+C content of 71·5 mol%, anteiso-C15 : 0 and C16 : 0 as the major fatty acids, MK-9(H4) as the major isoprenoid quinone, a peptidoglycan containing l-ornithine as the diamino acid and d-aspartic acid in the interpeptide bridge and phosphatidylglycerol as the only identified main polar lipid. The strain is aerobic to facultatively anaerobic, being capable of growth under strictly anaerobic conditions. Optimal growth occurs between pH values 9·0 and 10·0. On the basis of its distinct phylogenetic position and metabolic properties, strain 69B4T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas bogoriensis sp. nov. is proposed. The type strain is 69B4T (=DSM 16987T=CIP 108683T).

scholarly journals Leucobacter iarius sp. nov., in the family Microbacteriaceae

2007 ◽  
Vol 57 (4) ◽  
pp. 682-686 ◽  
Author(s):  
Vishal S. Somvanshi ◽  
Elke Lang ◽  
Peter Schumann ◽  
Rüdiger Pukall ◽  
R. M. Kroppenstedt ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Entomopathogenic Nematode ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Analysis ◽  
Cross Linkage ◽  
Diamino Acid ◽  
Bacterium Strain ◽  
Type Strains

A novel Gram-positive bacterium, strain 40T, was isolated in the course of identifying bacteria from infective juveniles of the entomopathogenic nematode Steinernema thermophilum. Based on 16S rRNA gene analysis, strain 40T was found to be related to the type strains of recognized species of the genus Leucobacter, family Microbacteriaceae. The 16S rRNA gene sequence similarity values of strain 40T and Leucobacter albus IAM 14851T, Leucobacter luti LMG 23118T, Leucobacter alluvii LMG 23117T, Leucobacter komagatae DSM 8803T, Leucobacter chromiireducens CIP 108389T and Leucobacter aridicollis CIP 108388T, respectively, were 97.3, 97.5, 97.6, 97.6, 97.6 and 98.5 %. Chemotaxonomic analysis also supported the affiliation of strain 40T to the genus Leucobacter: the major menaquinone was MK-11, the peptidoglycan cross-linkage was of the B-type, the cell wall diamino acid was l-diaminobutyric acid and the major fatty acids were anteiso-C15 : 0 (42 %), anteiso-C17 : 0 (34 %) and iso-C16 : 0 (16 %). Based upon the biochemical and genomic analyses, strain 40T is sufficiently distinct from the type strains of recognized Leucobacter species to warrant the description of a novel species, for which the name Leucobacter iarius sp. nov. is proposed. The type strain is strain 40T (=DSM 17402T=CIP 108831T).

scholarly journals Chthonomonas calidirosea gen. nov., sp. nov., an aerobic, pigmented, thermophilic micro-organism of a novel bacterial class, Chthonomonadetes classis nov., of the newly described phylum Armatimonadetes originally designated candidate division OP10

2011 ◽  
Vol 61 (10) ◽  
pp. 2482-2490 ◽  
Author(s):  
Kevin C.-Y. Lee ◽  
Peter F. Dunfield ◽  
Xochitl C. Morgan ◽  
Michelle A. Crowe ◽  
Karen M. Houghton ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Optimal Growth ◽  
Phylogenetic Position ◽  
Crystalline Cellulose ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
New Class ◽  
Candidate Division ◽  
Bacterium Strain ◽  
Heated Soil

An aerobic, saccharolytic, obligately thermophilic, motile, non-spore-forming bacterium, strain T49T, was isolated from geothermally heated soil at Hell’s Gate, Tikitere, New Zealand. On the basis of 16S rRNA gene sequence similarity, T49T is the first representative of a new class in the newly described phylum Armatimonadetes, formerly known as candidate division OP10. Cells of strain T49T stained Gram-negative and were catalase-positive and oxidase-negative. Cells possessed a highly corrugated outer membrane. The major fatty acids were 16 : 0, i17 : 0 and ai17 : 0. The G+C content of the genomic DNA was 54.6 mol%. Strain T49T grew at 50–73 °C with an optimum temperature of 68 °C, and at pH 4.7–5.8 with an optimum growth pH of 5.3. A growth rate of 0.012 h−1 was observed under optimal temperature and pH conditions. The primary respiratory quinone was MK-8. Optimal growth was achieved in the absence of NaCl, although growth was observed at NaCl concentrations as high as 2 % (w/v). Strain T49T was able to utilize mono- and disaccharides such as cellobiose, lactose, mannose and glucose, as well as branched or amorphous polysaccharides such as starch, CM-cellulose, xylan and glycogen, but not highly linear polysaccharides such as crystalline cellulose or cotton. On the basis of its phylogenetic position and phenotypic characteristics, we propose that strain T49T represents a novel bacterial genus and species within the new class Chthonomonadetes classis nov. of the phylum Armatimonadetes. The type strain of Chthonomonas calidirosea gen. nov., sp. nov. is T49T ( = DSM 23976T = ICMP 18418T).

scholarly journals Knoellia aerolata sp. nov., isolated from an air sample in Korea

2007 ◽  
Vol 57 (12) ◽  
pp. 2861-2864 ◽  
Author(s):  
Hang-Yeon Weon ◽  
Byung-Yong Kim ◽  
Peter Schumann ◽  
Reiner M. Kroppenstedt ◽  
Hyung-Jun Noh ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Mycolic Acids ◽  
Gene Sequence Analysis ◽  
Diamino Acid ◽  
Air Sample ◽  
Bacterium Strain ◽  
Ph Range ◽  
Type Strains

An aerobic, Gram-positive, non-motile, non-spore-forming, rod–coccus-shaped bacterium, strain 5317S-21T, was isolated from an air sample from Suwon city, Republic of Korea. The isolate was able to grow within a pH range of 5.0–9.0 and a temperature range of 5–35 °C and it tolerated up to 2 % (w/v) NaCl. The cell-wall peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The predominant isoprenoid quinone was MK-8(H4). The major polar lipids were phosphatidylinositol, phosphatidylethanolamine and diphosphatidylglycerol; phosphatidylglycerol and several unknown phospholipids were also detected. Mycolic acids were absent. The only whole-cell sugar was glucose. The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c and iso-C15 : 0. 16S rRNA gene sequence analysis indicated that strain 5317S-21T was related phylogenetically to members of the genus Knoellia, with 97.4 % sequence similarity to the type strains of Knoellia sinensis and Knoellia subterranea. The G+C content of the genomic DNA of strain 5317S-21T was 73 mol%. Levels of DNA–DNA relatedness between strain 5317S-21T and the type strains of Knoellia sinensis and Knoellia subterranea were 37 and 41 %, respectively. It was concluded that strain 5317S-21T represents a novel species of the genus Knoellia, for which the name Knoellia aerolata sp. nov. is proposed. The type strain is 5317S-21T (=KACC 20583T =DSM 18566T).

scholarly journals Brachybacterium squillarum sp. nov., isolated from salt-fermented seafood

2011 ◽  
Vol 61 (5) ◽  
pp. 1118-1122 ◽  
Author(s):  
Seong-Kyu Park ◽  
Min-Soo Kim ◽  
Mi-Ja Jung ◽  
Young-Do Nam ◽  
Eun-Jin Park ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Optimal Growth ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Polar Lipid Profile ◽  
Diamino Acid ◽  
Bacterium Strain ◽  
The Mean

A Gram-positive bacterium, strain M-6-3T, was isolated from salt-fermented seafood in Korea. The organism grew in 0–10 % (w/v) NaCl and at 25–37 °C, with optimal growth occurring in 5 % NaCl and at 28–30 °C. The peptidoglycan type was variation A4γ with meso-diaminopimelic acid as the diagnostic cell-wall diamino acid. The polar lipid profile of strain M-6-3T consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unknown glycolipid. Strain M-6-3T contained MK-7 as the major component of the quinone system and anteiso-C15 : 0 (62.1 %) as the predominant fatty acid. Based on 16S rRNA gene sequence similarity studies, strain M-6-3T was most closely related to Brachybacterium rhamnosum LMG 19848T (98.5 %). The G+C content of the genomic DNA was 71.5 mol% and the mean DNA–DNA hybridization value with reference strains was 14.32±2.0 %. Based on phenotypic, genotypic and phylogenetic analyses, it is proposed that strain M-6-3T represents a novel species for which the name Brachybacterium squillarum sp. nov. is proposed; the type strain is M-6-3T ( = KACC 14221T  = JCM 16464T).

scholarly journals Pectinatus brassicae sp. nov., a Gram-negative, anaerobic bacterium isolated from salty wastewater

2012 ◽  
Vol 62 (Pt_9) ◽  
pp. 2145-2149 ◽  
Author(s):  
Wen-wu Zhang ◽  
Ming-xu Fang ◽  
Hai-qin Tan ◽  
Xin-qi Zhang ◽  
Min Wu ◽  
...  
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Heterotrophic Bacterium ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Fermentation Products ◽  
Gram Negative ◽  
Content Type ◽  
Bacterium Strain

A novel Gram-negative, non-spore-forming, strictly anaerobic, heterotrophic bacterium, strain TYT, was isolated from salty pickle wastewater. Cells were rod-shaped with comb-like flagella, slightly curved and very variable in length. Optimal growth occurred at 28 °C and pH 6.5. Cells were resistant to up to 50 g NaCl l−1. Strain TYT produced acid from glycerol, sucrose, glucose, fructose and mannitol. The main fermentation products from glucose were acetic and propionic acids. Tests for acid phosphatase and naphthol-AS-BI-phosphohydrolase activities were positive. The major fatty acids were C14 : 0 DMA (18.7 %), C15 : 0 (15.4 %), anteiso-C18 : 1 (15.2 %), C11 : 0 (13.3 %) and summed feature 5 (C17 : 1ω7c and/or C17 : 2) (11.0 %). The DNA G+C content was 35.9 mol%. 16S rRNA gene sequence-based phylogenetic analysis indicated that strain TYT represented a novel species of the genus Pectinatus (sequence similarity to other members of the genus ranged from 93.2 to 94.8 %). Based on its phenotypic, genotypic and phylogenetic characteristics, strain TYT is proposed to represent a novel species, named Pectinatus brassicae sp. nov. (type strain TYT  = JCM 17499T  = DSM 24661T).

scholarly journals Oceanitalea nanhaiensis gen. nov., sp. nov., an actinobacterium isolated from seawater

2012 ◽  
Vol 62 (Pt_10) ◽  
pp. 2490-2494 ◽  
Author(s):  
Yingnan Fu ◽  
Qipei Li ◽  
Keshao Liu ◽  
Yongle Xu ◽  
Yanan Wang ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Diamino Acid ◽  
Unknown Phospholipid ◽  
Phosphatidylinositol Mannosides ◽  
Type Strains ◽  
The 16S Rrna Gene

A Gram-positive, motile, short-rod-shaped bacterium, designated strain JLT1488T, was isolated from the South China Sea and investigated in a taxonomic study using a polyphasic approach. The peptidoglycan type determined for strain JLT1488T was A4α with lysine as the diagnostic cell-wall diamino acid and an interpeptide bridge of l-Lys–l-Glu. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unknown glycolipid and an unknown phospholipid. The only detected menaquinone was MK-8(H4), and the major fatty acids were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c) , C16 : 0 and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c); significant amounts of C12 : 0 3-OH, C10 : 0 and C19 : 0 cyclo ω8c were also present. The G+C content of the genomic DNA was 62.3 mol%. Comparison of the 16S rRNA gene sequence of strain JLT1488T with those of related type strains demonstrated that it represented a novel lineage within the family Bogoriellaceae , suborder Micrococcineae , being closely related to species of the genera Georgenia , Bogoriella and Cellulomonas (94.6–96.8 % sequence similarity). These results demonstrate that strain JLT1488T is a member of a new genus, for which the name Oceanitalea nanhaiensis gen. nov., sp. nov. is proposed. The type strain of the type species is JLT1488T ( = JCM 17755T = CGMCC 1.10826T).

scholarly journals Allocatelliglobosispora scoriae gen. nov., sp. nov., isolated from volcanic ash

2011 ◽  
Vol 61 (2) ◽  
pp. 264-270 ◽  
Author(s):  
Dong Wan Lee ◽  
Soon Dong Lee
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Volcanic Ash ◽  
Sequence Similarity ◽  
Aerial Mycelium ◽  
Rrna Gene ◽  
The Family ◽  
Diamino Acid ◽  
Type Strains ◽  
The 16S Rrna Gene

A novel actinomycete, designated strain Sco-B14T, was isolated from volcanic ash collected near Darangshi Oreum (a parasitic or satellite volcano) in Jeju, Republic of Korea. The organism formed well-developed, branched substrate mycelium, on which short chains of non-motile spores were arranged singly or in clusters. Aerial mycelium was not produced. Globose bodies were observed. The reverse colour of colonies was light brown to brown. Diffusible pigments were produced on ISP medium 3 and oatmeal-nitrate agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Sco-B14T formed a lineage within the family Micromonosporaceae and was distinct from established genera. The 16S rRNA gene sequence similarity of strain Sco-B14T to members of related genera of the family was 95.0–95.7 % to type strains of Catellatospora species, 94.7 % to Hamadaea tsunoensis IMSNU 22005T, 94.7 % to Longispora albida K97-0003T and 94.0 % to Catelliglobosispora koreensis LM 042T. 3-Hydroxydiaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. Whole-cell sugars were glucose, rhamnose, ribose, xylose, arabinose, galactose and mannose. The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The menaquinone profile contained MK-10(H4) (49 %), MK-9(H4) (24 %), MK-10(H6) (18 %) and MK-9(H6) (9 %). The predominant fatty acids were iso-C15 : 0 and C17 : 0. The DNA G+C content was 70.1 mol%. The combination of chemotaxonomic and phylogenetic data clearly separated the isolate from the type strains of all genera in the family Micromonosporaceae. On the basis of the phylogenetic and chemotaxonomic data presented in this paper, strain Sco-B14T is considered to represent a novel species of a new genus in the family Micromonosporaceae, for which the name Allocatelliglobosispora scoriae gen. nov., sp. nov. is proposed. The type strain of Allocatelliglobosispora scoriae is Sco-B14T (=KCTC 19661T =DSM 45362T).

scholarly journals Novosphingobium gossypii sp. nov., isolated from Gossypium hirsutum

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2831-2837 ◽  
Author(s):  
Peter Kämpfer ◽  
Karin Martin ◽  
John A. McInroy ◽  
Stefanie P. Glaeser
Keyword(s):  
Gossypium Hirsutum ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
High Sequence Similarity ◽  
Polar Lipid Profile ◽  
Gene Sequence Analysis ◽  
Bacterium Strain ◽  
Type Strains ◽  
Sequence Similarities

A Gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain JM-1396T) producing a yellow pigment, was isolated from the healthy internal stem tissue of post-harvest cotton (Gossypium hirsutum, cultivar ‘DES-119’) grown at the Plant Breeding Unit at the E. V. Smith Research Center in Tallassee (Macon county), AL, USA. 16S rRNA gene sequence analysis of strain JM-1396T showed high sequence similarity values to the type strains of Novosphingobium mathurense, Novosphingobium panipatense (both 98.6 %) and Novosphingobium barchaimii (98.5 %); sequence similarities to all other type strains of species of the genus Novosphingobium were below 98.3 %. DNA–DNA pairing experiments of the DNA of strain JM-1396T and N. mathurense SM117T, N. panipatense SM16T and N. barchaimii DSM 25411T showed low relatedness values of 8 % (reciprocal 7 %), 24 % (reciprocal 26 %) and 19 % (reciprocal 25 %), respectively. Ubiquinone Q-10 was detected as the dominant quinone; the fatty acids C18 : 1ω7c (71.0 %) and the typical 2-hydroxy fatty acid, C14 : 0 2-OH (11.7 %), were detected as typical components. The polar lipid profile contained the diagnostic lipids diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and phosphatidylcholine. The polyamine pattern contained the major compound spermidine and only minor amounts of other polyamines. All these data revealed that strain JM-1396T represents a novel species of the genus Novosphingobium. For this reason we propose the name Novosphingobium gossypii sp. nov. with the type strain JM-1396T ( = LMG 28605T = CCM 8569T = CIP 110884T).

scholarly journals Antricoccus suffuscus gen. nov., sp. nov., isolated from a natural cave

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4410-4416 ◽  
Author(s):  
Soon Dong Lee
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Novel Species ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Diamino Acid ◽  
Unknown Phospholipid

A novel actinobacterium, designated strain C4-31T, was isolated from soil collected from a cave. Cells were aerobic, Gram-reaction-positive, oxidase-negative, catalase-positive and non-motile cocci. Comparison of 16S rRNA gene sequences showed that the organism occupied a distinct phylogenetic position within the suborder Frankineae, with sequence similarity values of less than 93.2 % to members of this suborder. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major menaquinone was MK-9(H4). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unknown aminophospholipid and an unknown phospholipid. The major fatty acids were iso-C16 : 0, C17 : 1ω6c and C16 : 0. The G+C content of the DNA was 62.8 mol%. On the basis of morphological and chemotaxonomic data as well as phylogenetic evidence, strain C4-31T ( = KCTC 39556T = DSM 100065T) is considered to represent the type strain of a novel species of a new genus in the suborder Frankineae, for which the name Antricoccus suffuscus gen. nov., sp. nov. is proposed.

scholarly journals Paenibacillus physcomitrellae sp. nov., isolated from the moss Physcomitrella patens

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3400-3406 ◽  
Author(s):  
Xun Zhou ◽  
Guan Nan Guo ◽  
Le Qi Wang ◽  
Su Lan Bai ◽  
Chun Li Li ◽  
...  
Keyword(s):  
Physcomitrella Patens ◽  
Optimal Growth ◽  
Major Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
Phenotypic Characteristics ◽  
Diamino Acid ◽  
Beijing China ◽  
The 16S Rrna Gene

A Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain XBT, was isolated from Physcomitrella patens growing in Beijing, China. The isolate was identified as a member of the genus Paenibacillus based on phenotypic characteristics and phylogenetic inferences. The novel strain was spore-forming, motile, catalase-negative and weakly oxidase-positive. Optimal growth of strain XBT occurred at 28°C and pH 7.0–7.5. The major polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unidentified components, including one phospholipid, two aminophospholipids, three glycolipids, one aminolipid and one lipid. The predominant isoprenoid quinone was MK-7. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major fatty acid components (>5 %) were anteiso-C15 : 0 (51.2 %), anteiso-C17 : 0 (20.6 %), iso-C16 : 0 (8.3 %) and C16 : 0 (6.7 %). The G+C content of the genomic DNA was 53.3 mol%. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that strain XBT fell within the evolutionary distances encompassed by the genus Paenibacillus; its closest phylogenetic neighbour was Paenibacillus yonginensis DCY84T (96.6 %). Based on phenotypic, chemotaxonomic and phylogenetic properties, strain XBT is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus physcomitrellae sp. nov., is proposed. The type strain is XBT ( = CGMCC 1.15044T = DSM 29851T).

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