scholarly journals Deinococcus metalli sp. nov., isolated from an abandoned lead-zinc mine

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3457-3461 ◽  
Author(s):  
Guang-Da Feng ◽  
Yong-Hong Wang ◽  
Yan-Xuan Li ◽  
Hong-Hui Zhu
Keyword(s):  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Taxonomic Study ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Gram Staining ◽  
Lead Zinc ◽  
Zinc Mine ◽  
Major Respiratory Quinone ◽  
Deinococcus Ficus

An aerobic, non-motile and Gram-staining-positive bacterial strain (1PNM-19T) was isolated from a lead-zinc ore in an abandoned mine and was investigated in a taxonomic study using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 1PNM-19T was affiliated to the genus Deinococcus and most closely related to Deinococcus aquatilis DSM 23025T and Deinococcus ficus DSM 19119T. The major respiratory quinone was determined to be menaquinone 8 (MK-8) and the major fatty acids contained summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. A complex polar lipid profile consisted of different unidentified glycolipids and polar lipids, two unidentified aminolipids, an unidentified phosphoglycolipid, phospholipid and aminophospholipid. The genomic DNA G+C content of strain 1PNM-19T was 71.7 ± 0.1 mol%. Based on data from this taxonomic study, strain 1PNM-19T represents a novel species of the genus Deinococcus, for which the name Deinococcus metalli sp. nov. is proposed. The type strain is 1PNM-19T ( = GIMCC 1.654T = CCTCC AB 2014198T = DSM 27521T).

scholarly journals Paracoccus isoporae sp. nov., isolated from the reef-building coral Isopora palifera

2011 ◽  
Vol 61 (5) ◽  
pp. 1138-1143 ◽  
Author(s):  
Ming-Hui Chen ◽  
Shih-Yi Sheu ◽  
Chaolun Allen Chen ◽  
Jih-Terng Wang ◽  
Wen-Ming Chen
Keyword(s):  
Phylogenetic Analyses ◽  
Optimal Growth ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Major Respiratory Quinone ◽  
Taxonomic Approach ◽  
Isopora Palifera

A bacterial strain, designated SW-3T, was isolated from the reef-building coral Isopora palifera, from Southern Taiwan, and was characterized by using a polyphasic taxonomic approach. Cells of strain SW-3T were Gram-reaction-negative, aerobic, rod-shaped and motile by means of monopolar flagella and formed cream–white colonies. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SW-3T belonged to the genus Paracoccus and its most closely related neighbours were P. aestuarii B7T, P. homiensis DD-R11T, P. marinus KKL-A5T, P. denitrificans DSM 413T and P. zeaxanthinifaciens R-1512T, with sequence similarities of 96.8, 96.6, 96.1 , 95.8 and 94.9 %, respectively. Strain SW-3T exhibited optimal growth at 25–30 °C and pH 9–10 and in 3–5 % (w/v) NaCl. The predominant cellular fatty acid was summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 75 %). The major respiratory quinone was Q-10. The polar lipid profile consisted of a mixture of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and several uncharacterized polar lipids. The DNA G+C content of strain SW-3T was 63.7 mol%. The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from recognized species of the genus Paracoccus. It is evident from the genotypic, phenotypic and chemotaxonomic data that strain SW-3T represents a novel species of the genus Paracoccus, for which the name Paracoccus isoporae sp. nov. is proposed. The type strain is SW-3T ( = LMG 25205T  = BCRC 17967T).

Rhodocytophaga aerolata gen. nov., sp. nov., a new member of the family Cytophagaceae isolated from air

2010 ◽  
Vol 60 (7) ◽  
pp. 1554-1558 ◽  
Author(s):  
Rangasamy Anandham ◽  
Hang-Yeon Weon ◽  
Soo-Jin Kim ◽  
Yi-Seul Kim ◽  
Soon-Wo Kwon
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
The Family ◽  
Major Respiratory Quinone

A strictly aerobic, Gram-staining-negative, oxidase- and catalase-positive, non-motile, rod-shaped bacterium, designated strain 5416T-29T, was isolated from air and was characterized by using a polyphasic approach. Colonies were reddish pink and circular with entire margins. Flexirubin-type pigments were absent. The strain formed a distinct phylogenetic lineage within the family Cytophagaceae of the phylum Bacteroidetes. Strain 5416T-29T did not show more than 88 % 16S rRNA gene sequence similarity to the type strain of any recognized species. The major cellular fatty acids were C16 : 1 ω5c, iso-C17 : 0 3-OH and iso-C15 : 0. The polar lipids were phosphatidylethanolamine, one unknown amino lipid and several unknown polar lipids. Menaquinone-7 (MK-7) was the major respiratory quinone. The G+C content of the DNA of strain 5416T-29T was 45.5 mol%. Results of phenotypic and phylogenetic analyses clearly indicate that strain 5416T-29T represents a novel species of a new genus in the family Cytophagaceae, for which the name Rhodocytophaga aerolata gen. nov., sp. nov. is proposed. The type strain of Rhodocytophaga aerolata is 5416T-29T (=KACC 12507T =DSM 22190T).

scholarly journals Tenacibaculum jejuense sp. nov., isolated from coastal seawater

2012 ◽  
Vol 62 (2) ◽  
pp. 414-419 ◽  
Author(s):  
You-Sung Oh ◽  
Hyung-Yeel Kahng ◽  
Dong-Heon Lee ◽  
Sun Bok Lee
Keyword(s):  
Phylogenetic Analyses ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
The Family ◽  
Major Respiratory Quinone ◽  
Type Strains

A Gram-staining-negative, strictly aerobic and rod-shaped bacterium, designated strain CNURIC013T, was isolated from seawater collected on the coast of Jeju Island, South Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CNURIC013T belonged to the genus Tenacibaculum, within the family Flavobacteriaceae. Sequence similarities between the novel strain and the type strains of recognized species of the genus Tenacibaculum were 93.6–96.0 %, the highest value being with Tenacibaculum litopenaei B-IT (96 %). The DNA G+C content of the novel strain was 34.5 mol% and the major respiratory quinone was menaquinone-6. The major fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH; 26.0 %), iso-C15 : 0 (24.4 %), iso-C15 : 1 G (18.5 %) and iso-C17 : 0 3-OH (8.1 %). The polar lipids consisted of phosphatidylethanolamine, one unknown aminophospholipid and nine unknown polar lipids. On the basis of the phenotypic, phylogenetic and genotypic data, strain CNURIC013T represents a novel species within the genus Tenacibaculum, for which the name Tenacibaculum jejuense sp. nov. is proposed. The type strain is CNURIC013T ( = KCTC 22618T = JCM 15975T).

scholarly journals Fulvivirga imtechensis sp. nov., a member of the phylum Bacteroidetes

2012 ◽  
Vol 62 (Pt_9) ◽  
pp. 2213-2217 ◽  
Author(s):  
Nupur ◽  
Shalley Sharma ◽  
Pradip Kumar Singh ◽  
Korpole Suresh ◽  
Pinnaka Anil Kumar
Keyword(s):  
Sequence Similarity ◽  
Rrna Gene ◽  
The Novel ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
The Family ◽  
Major Respiratory Quinone

A novel, Gram-staining-negative, yellow-coloured, rod-shaped, obligately aerobic, non-motile bacterium, designated strain AK7T, was isolated from seawater collected on the coast at Visakhapatnam, Andhra Pradesh, India. The predominant fatty acids of the novel strain were iso-C15 : 0, iso-C15 : 0 3-OH, C16 : 1ω5c, iso-C17 : 0 3-OH and summed features 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The major respiratory quinone was MK-7 and the polar lipid profile comprised phosphatidylethanolamine, two unidentified aminolipids and four other unidentified lipids. In phylogenetic analysis based on 16S rRNA gene sequences, strain AK7T appeared most closely related to Fulvivirga kasyanovii KMM 6220T (95.9 % sequence similarity), a member of the family Flammeovirgaceae in the phylum Bacteroidetes . The genomic DNA G+C content of strain AK7T was 55.1 mol%. Based on the morphological, biochemical, physiological, chemotaxonomic and phylogenetic evidence, strain AK7T represents a novel species of the genus Fulvivirga for which the name Fulvivirga imtechensis sp. nov. is proposed. The type strain is AK7T ( = MTCC 11053T  = JCM 17390T).

scholarly journals Arcicella aurantiaca sp. nov., isolated from stream water

2010 ◽  
Vol 60 (12) ◽  
pp. 2979-2983 ◽  
Author(s):  
Shih-Yi Sheu ◽  
Cho-Song Yang ◽  
Ming-Hui Chen ◽  
A. B. Arun ◽  
Chiu-Chung Young ◽  
...  
Keyword(s):  
Stream Water ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Phenotypic Data ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Major Respiratory Quinone ◽  
Southern Taiwan ◽  
Taxonomic Approach

A bacterial strain designated TNR-18T was isolated from stream water in southern Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain TNR-18T were Gram-stain-negative, strictly aerobic, non-motile and vibrioid, and the strain formed orange colonies. Growth occurred at 10–30 °C, with 0–0.2 % NaCl and at pH 6.0–10.0. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TNR-18T belonged to the genus Arcicella; its closest neighbours were Arcicella rosea TW5T and Arcicella aquatica NO-502T, with respective sequence similarities of 97.9 and 97.8 %. Predominant cellular fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c; 37.7 %), C16 : 1 ω5c (13.0 %) and iso-C15 : 0 (11.1 %). The major respiratory quinone was MK-7. The polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized polar lipids. The DNA G+C content was 39.9 mol%. On the basis of the phylogenetic and phenotypic data, strain TNR-18T should be classified as representing a novel species, for which the name Arcicella aurantiaca sp. nov. is proposed. The type strain is TNR-18T (=LMG 25207T =BCRC 17969T).

scholarly journals Sphingomonas gei sp. nov., isolated from roots of Geum aleppicum

2015 ◽  
Vol 65 (Pt_4) ◽  
pp. 1160-1166 ◽  
Author(s):  
Lingfang Zhu ◽  
Meiru Si ◽  
Changfu Li ◽  
Kaiyun Xin ◽  
Chaoqiong Chen ◽  
...  
Keyword(s):  
Type Species ◽  
Phylogenetic Analyses ◽  
Shaanxi Province ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Taxonomic Study ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type

A yellow-pigmented bacterium, designated strain ZFGT-11T, was isolated from roots of Geum aleppicum Jacq. collected from Taibai Mountain in Shaanxi Province, north-west China, and was subjected to a taxonomic study by using a polyphasic approach. Cells of strain ZFGT-11T were Gram-stain-negative, strictly aerobic rods that were surrounded by a thick capsule and were motile by means of a single polar flagellum. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ZFGT-11T was a member of the genus Sphingomonas and was closely related to Sphingomonas naasensis KACC 16534T (97.6 % similarity), Sphingomonas kyeonggiense JCM 18825T (96.8 %), Sphingomonas asaccharolytica IFO 15499T (96.7 %) and Sphingomonas leidyi DSM 4733T (96.6 %). The predominant respiratory quinone was ubiquinone-10 (Q-10) and the major cellular fatty acids were summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C17 : 1ω6c, C14 : 0 2-OH, C16 : 0 and C15 : 0 2-OH. The major polyamine of strain ZFGT-11T was sym-homospermidine. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, sphingoglycolipid, two unidentified aminoglycolipids, two unidentified phospholipids and two unidentified lipids were detected in the polar lipid profile. The DNA G+C content was 66.8 mol%. DNA–DNA relatedness for strain ZFGT-11T with respect to its closest phylogenetic relative S. naasensis KACC 16534T was 26.2±4.8 % (mean±sd). On the basis of data from the present polyphasic taxonomic study, strain ZFGT-11T is considered to represent a novel species of the genus Sphingomonas , for which the name Sphingomonas gei sp. nov. is proposed. The type strain is ZFGT-11T ( = CCTCC AB 2013306T = KCTC 32449T = LMG 27608T).

scholarly journals Vogesella amnigena sp. nov., isolated from a freshwater river

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3634-3640 ◽  
Author(s):  
Wen-Ming Chen ◽  
Jhen-Ci Chen ◽  
Chi Wang ◽  
Cheng-Wen Huang ◽  
Shih-Yi Sheu
Keyword(s):  
Dna Hybridization ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Optimum Ph ◽  
Major Respiratory Quinone ◽  
Freshwater River

A bacterial strain, designated Npb-02T, was isolated from a freshwater river in Taiwan and characterized in a taxonomic study using a polyphasic approach. Cells of strain Npb-02T were Gram-stain-negative, aerobic, poly-β-hydroxybutyrate-accumulating, rod-shaped and non-motile. Growth occurred at 15–40 °C (optimum 25–30 °C), at pH 7.0–8.0 (optimum pH 7.0) and with 0–1 % NaCl (optimum 0.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-02T belonged to the genus Vogesella and was most closely related to Vogesella perlucida DS-28T with sequence similarity of 98.3 %. Strain Npb-02T contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0 as the major fatty acids. The major respiratory quinone was Q-8.The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminophospholipid and an uncharacterized phospholipid. The genomic DNA G+C content of strain Npb-02T was 64.1 mol%. The DNA–DNA hybridization values for strain Npb-02T with Vogesella perlucida DS-28T, Vogesella mureinivorans 389T and Vogesella lacus GR13T were less than 25 %. On the basis of phylogenetic inference and phenotypic data, strain Npb-02T represents a novel species of the genus Vogesella, for which the name Vogesella amnigena sp. nov. is proposed. The type strain is Npb-02T ( = BCRC 80887T = LMG 28419T = KCTC 42195T).

Orbus sasakiae sp. nov., a bacterium isolated from the gut of the butterfly Sasakia charonda, and emended description of the genus Orbus

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1766-1770 ◽  
Author(s):  
Joon Yong Kim ◽  
Jina Lee ◽  
Na-Ri Shin ◽  
Ji-Hyun Yun ◽  
Tae Woong Whon ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
Bacterium Strain ◽  
Sasakia Charonda

A novel Gram-stain-negative, facultatively anaerobic, non-motile and coccus-shaped bacterium, strain C7T, was isolated from the gut of the butterfly Sasakia charonda. Strain C7T grew optimally at 20–25 °C, at pH 7–8 and with 1 % (w/v) NaCl. The strain was negative for oxidase activity but positive for catalase activity. The 16S rRNA gene sequences of strain C7T and Orbus hercynius CN3T shared 96.8 % similarity. The major fatty acids identified were C14 : 0, C16 : 0, C18 : 1ω7c and summed feature 2 (comprising C14 : 0 3-OH/iso-C16 : 1). The major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids of strain C7T were phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid and two unidentified aminophospholipids. The G+C content of the genomic DNA extracted from strain C7T was 32.1 mol%. Taken together, the phenotypic, genotypic and phylogenetic analyses indicate that strain C7T represents a novel species of the genus Orbus , for which the name Orbus sasakiae sp. nov. is proposed. The type strain is C7T ( = KACC 16544T = JCM 18050T). An emended description of the genus Orbus is provided.

Flavobacterium chungbukense sp. nov., isolated from soil

2011 ◽  
Vol 61 (11) ◽  
pp. 2734-2739 ◽  
Author(s):  
Chae-Sung Lim ◽  
Yong-Sik Oh ◽  
Jae-Kwan Lee ◽  
A-Rum Park ◽  
Jae-Soo Yoo ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
Flavobacterium Johnsoniae ◽  
The Family ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

A yellow-pigmented, Gram-staining-negative, non-motile, strictly aerobic and rod-shaped bacterium, designated CS100T, was isolated from soil in Chungbuk, Korea. Phylogenetic analysis and comparative studies based on the 16S rRNA gene sequence showed that strain CS100T belonged to the genus Flavobacterium in the family Flavobacteriaceae. Strain CS100T showed the highest sequence similarities to Flavobacterium glaciei JCM 13953T (97.6 %) and Flavobacterium johnsoniae KACC 11410T (97.1 %). Sequence similarity to other members of the genus Flavobacterium was 91.5–97.0 %. Growth occurred at 4–30 °C, at pH 5.0–9.0 and in the presence of 0–2 % (w/v) NaCl. Flexirubin-type pigments were produced. Menaquinone-6 (MK-6) was the major respiratory quinone and the major fatty acids were iso-C15 : 0 (17.3 %), summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c, 15.5 %) and C16 : 0 (11.8 %). The DNA G+C content was 36.4 mol%. Strain CS100T hydrolysed skimmed milk and gelatin, but not chitin or pectin, and showed oxidase and catalase activities. DNA–DNA relatedness was 3.0 % with F. glaciei JCM 13953T and 11.5 % with F. johnsoniae KACC 11410T. On the basis of the evidence from this study, strain CS100T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium chungbukense sp. nov. is proposed. The type strain is CS100T ( = KACC 15048T = JCM 17386T).

Description of Sandaracinobacter Hominis Sp. Nov., Isolated From Human Skin

2021 ◽  
Author(s):  
Ping-hua Qu ◽  
Hai-min Luo ◽  
Jun-hui Feng ◽  
Lei Dong ◽  
Song Li ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Skin Sample ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Separate Branch ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene

Abstract Strain SZY PN-1T, representing a novel Gram-negative, aerobic, non-motile, rod-shaped and yellow-pigmented bacterium, was isolated from a skin sample of a healthy Chinese people. Growth of SZY PN-1T optimally occurred at pH 7.0, at 30 ºC and tolerate up to 1.0 % (w/v) NaCl. According to the absorption spectrum, carotenoid was present in the cells. Comparative analysis of the 16S rRNA gene revealed that strain SZY PN-1T shared high similarities with Sandaracinobacter sibiricus RB16-17T (97.1 %) and Sandaracinobacter neustonicus JCM 30734T (96.6 %), respectively. Phylogenetic analysis of 16S rRNA gene sequences together with protein-concatamer tree showed that SZY PN-1T formed a separate branch within the genus Sandaracinobacter. The DNA G+C content of the strain SZY PN-1T was 65.0 % (genome). The polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, two sphingoglycolipids, diphosphatidylglycerol, five unidentified glycolipids and seven unidentified lipids. The predominant fatty acids (> 10.0 %) were identified as C18:1 ω7c and/or C18:1 ω6c, C17:1 ω6c, C16:1 ω7c and/or C16:1 ω6c. The major respiratory quinone was ubiquinone Q-10. Based on the phenotypic and genotypic features, we proposed Sandaracinobacter hominis sp. nov. with type strain SZY PN-1T (= KCTC 82150T = NBRC 114675T).

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