Isolation of an Adoxophyes orana granulovirus (AdorGV) occlusion body morphology mutant: biological activity, genome sequence and relationship to other isolates of AdorGV

ABSTRACTWith the aim of fabricating highly integrated protein microarrays, functional proteins were occluded into a protein crystal, consisting of a proteinaceous occlusion body termed polyhedrin, and the crystals were individually fixed onto a polymer film by using laser trapping and bonding techniques. Individual crystals were trapped by the 1064 nm beam of a Nd3+:YAG laser and placed to the film. The bonding to the film was achieved by irradiating with the single shot of the 800 nm beam of a femtosecond Ti:sapphire laser. Enhanced green fluorescent protein (EGFP) was occluded into the polyhedrin crystal and the crystals were patterned and observed by florescence imaging and single crystal florescence spectroscopy. From these experiments, the biological activity of the bonded crystal was confirmed and their potential as element in protein microarrays was discussed.

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Morphological properties of the occlusion body of Adoxophyes orana granulovirus

Journal of Invertebrate Pathology ◽

10.1016/j.jip.2018.03.016 ◽

2018 ◽

Vol 154 ◽

pp. 58-64

Author(s):

Keiko Tsuruta ◽

Jörg T. Wennmann ◽

Yasuhisa Kunimi ◽

Maki N. Inoue ◽

Madoka Nakai

Keyword(s):

Morphological Properties ◽

Occlusion Body ◽

Adoxophyes Orana

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Draft Genome Sequence of Bacillus subtilis ALBA01, a Strain with Antagonistic Activity against the Soilborne Fungal Pathogen of Onion Setophoma terrestris

Genome Announcements ◽

10.1128/genomea.00455-16 ◽

2016 ◽

Vol 4 (3) ◽

Cited By ~ 4

Author(s):

Andrea G. Albarracín Orio ◽

Romina A. Tobares ◽

Daniel A. Ducasse ◽

Andrea M. Smania

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Biological Activity ◽

Genome Sequence ◽

Fungal Pathogen ◽

Biological Control Agent ◽

Draft Genome ◽

Control Agent ◽

Antagonistic Activity ◽

Setophoma Terrestris

Bacillus subtilis is a nonpathogenic bacterium that lives in soil and has long been used as biological control agent in agriculture. Here, we report the genome sequence of a B. subtilis strain isolated from rhizosphere of onion that shows strong biological activity against the soilborne fungal pathogen Setophoma terrestris .

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Virus-Like Particles in a Human Breast Cancer: Structural Similarity to Previously Reported Particles

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072332 ◽

1973 ◽

Vol 31 ◽

pp. 378-379

Author(s):

G. Kasnic ◽

S. E. Stewart ◽

C. Urbanski

Keyword(s):

Breast Cancer ◽

Biological Activity ◽

Human Breast Cancer ◽

Osteogenic Sarcoma ◽

Human Tumor ◽

Structural Similarity ◽

Cell Tumor ◽

Human Breast ◽

Human Tumor Cell ◽

Type Particle

We have reported the maturation of an intracisternal A-type particle in murine plasma cell tumor cultures and three human tumor cell cultures (rhabdomyosarcoma, lung adenocarcinoma, and osteogenic sarcoma) after IUDR-DMSO activation. In all of these studies the A-type particle seems to develop into a form with an electron dense nucleoid, presumably mature, which is also intracisternal. A similar intracisternal A-type particle has been described in leukemic guinea pigs. Although no biological activity has yet been demonstrated for these particles, on morphologic grounds, and by the manner in which they develop within the cell, they may represent members of the same family of viruses.

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Effects of Vincristine on Endothelial Microtubules in the Spinal Cord

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100090270 ◽

1976 ◽

Vol 34 ◽

pp. 58-59

Author(s):

John L. Beggs ◽

John D. Waggener ◽

Wanda Miller

Keyword(s):

Spinal Cord ◽

Biological Activity ◽

Horseradish Peroxidase ◽

Transport Mechanism ◽

Vasogenic Edema ◽

Vesicular Transport ◽

Close Proximity

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.

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