Draft Genome Sequence of
                    Bacillus subtilis
                    ALBA01, a Strain with Antagonistic Activity against the Soilborne Fungal Pathogen of Onion
                    Setophoma terrestris

Bacillus subtilis is a nonpathogenic bacterium that lives in soil and has long been used as biological control agent in agriculture. Here, we report the genome sequence of a B. subtilis strain isolated from rhizosphere of onion that shows strong biological activity against the soilborne fungal pathogen Setophoma terrestris .

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Identification of the Fungal Pathogens of Postharvest Disease on Peach Fruits and the Control Mechanisms of Bacillus subtilis JK-14

Toxins ◽

10.3390/toxins11060322 ◽

2019 ◽

Vol 11 (6) ◽

pp. 322 ◽

Cited By ~ 11

Author(s):

Shuwu Zhang ◽

Qi Zheng ◽

Bingliang Xu ◽

Jia Liu

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Fungal Pathogens ◽

Biological Control Agent ◽

Storage Period ◽

Control Agent ◽

Antagonistic Activity ◽

Postharvest Disease ◽

Economic Losses ◽

Control Mechanisms

Postharvest fungal disease is one of the significant factors that limits the storage period and marketing life of peaches, and even result in serious economic losses worldwide. Biological control using microbial antagonists has been explored as an alternative approach for the management of postharvest disease of fruits. However, there is little information available regarding to the identification the fungal pathogen species that cause the postharvest peach diseases and the potential and mechanisms of using the Bacillus subtilis JK-14 to control postharvest peach diseases. In the present study, a total of six fungal isolates were isolated from peach fruits, and the isolates of Alternaria tenuis and Botrytis cinerea exhibited the highest pathogenicity and virulence on the host of mature peaches. In the culture plates, the strain of B. subtilis JK-14 showed the significant antagonistic activity against the growth of A. tenuis and B. cinerea with the inhibitory rates of 81.32% and 83.45% at 5 days after incubation, respectively. Peach fruits treated with different formulations of B. subtilis JK-14 significantly reduced the mean disease incidences and lesion diameters of A. tenuis and B. cinerea. The greatest mean percent reduction of the disease incidences (81.99% and 71.34%) and lesion diameters (82.80% and 73.57%) of A. tenuis and B. cinerea were obtained at the concentration of 1 × 107 CFU mL−1 (colony forming unit, CFU). Treatment with the strain of B. subtilis JK-14 effectively enhanced the activity of the antioxidant enzymes-superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in A. tenuis and B. cinerea inoculated peach fruits. As such, the average activities of SOD, POD and CAT were increased by 36.56%, 17.63% and 20.35%, respectively, compared to the sterile water treatment. Our results indicate that the isolates of A. tenuis and B. cinerea are the main pathogens that cause the postharvest peach diseases, and the strain of B. subtilis JK-14 can be considered as an environmentally-safe biological control agent for the management of postharvest fruits diseases. We propose the possible mechanisms of the strain of B. subtilis JK-14 in controlling of postharvest peach diseases.

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Draft Genome Sequence of the Mycoparasitic Oomycete Pythium periplocum Strain CBS 532.74

Genome Announcements ◽

10.1128/genomea.00057-17 ◽

2017 ◽

Vol 5 (12) ◽

Cited By ~ 5

Author(s):

Sandeep K. Kushwaha ◽

Ramesh R. Vetukuri ◽

Laura J. Grenville-Briggs

Keyword(s):

Biological Control ◽

Genome Sequence ◽

Biological Control Agent ◽

Draft Genome ◽

Pathogenic Fungi ◽

Control Agent ◽

Draft Genome Sequence ◽

Plant Pathogenic Fungi ◽

Protein Coding ◽

Protein Coding Genes

ABSTRACT The oomycete Pythium periplocum is an aggressive mycoparasite of a number of plant pathogenic fungi and oomycetes and therefore has potential as a biological control agent. Here, we report the first draft genome sequence of P. periplocum, which comprises 35.89 Mb. It contains 1,043 scaffolds and 14,399 predicted protein-coding genes.

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Draft Genome Sequence of Bacillus amyloliquefaciens Strain CB, a Biological Control Agent and Plant Growth-Promoting Bacterium Isolated From Cotton (Gossypium L.) Rhizosphere in Coimbatore, Tamil Nadu, India

Frontiers in Genetics ◽

10.3389/fgene.2021.704165 ◽

2021 ◽

Vol 12 ◽

Author(s):

Nakkeeran Sevugapperumal ◽

Vimalkumar S. Prajapati ◽

Vanthana Murugavel ◽

Renukadevi Perumal

Keyword(s):

Biological Control ◽

Plant Growth ◽

Genome Sequence ◽

Bacillus Amyloliquefaciens ◽

Tamil Nadu ◽

Biological Control Agent ◽

Draft Genome ◽

Control Agent ◽

Plant Growth Promoting ◽

Growth Promoting

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Draft Genome Sequence of the Mycoparasitic Oomycete Pythium oligandrum Strain CBS 530.74

Genome Announcements ◽

10.1128/genomea.00346-17 ◽

2017 ◽

Vol 5 (21) ◽

Cited By ~ 7

Author(s):

Sandeep K. Kushwaha ◽

Ramesh R. Vetukuri ◽

Laura J. Grenville-Briggs

Keyword(s):

Biological Control ◽

Genome Sequence ◽

Biological Control Agent ◽

Draft Genome ◽

Control Agent ◽

Draft Genome Sequence ◽

Pythium Oligandrum ◽

Protein Coding ◽

Protein Coding Genes

ABSTRACT The oomycete Pythium oligandrum is a mycoparasite and licenced biological control agent. Here, we report the draft genome sequence of P. oligandrum strain CBS 530.74, which is 36.80 Mb. It contains 341 scaffolds and 11,647 predicted protein-coding genes. As reported for plant-pathogenic Pythium species, RXLR-type effector sequences are absent.

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Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh

The Scientific World JOURNAL ◽

10.1100/2012/723293 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

M. M. Rahman ◽

M. E. Ali ◽

A. A. Khan ◽

A. M. Akanda ◽

Md. Kamal Uddin ◽

...

Keyword(s):

Biological Control ◽

Biological Control Agent ◽

Erwinia Carotovora ◽

Soft Rot ◽

Control Agent ◽

Antagonistic Activity ◽

Antagonistic Effect ◽

Antagonistic Bacteria ◽

And Storage

A total of 91 isolates of probable antagonistic bacteria of potato soft rot bacteriumErwinia carotovorasubsp.carotovora(Ecc) were extracted from rhizospheres and endophytes of various crop plants, different soil varieties, and atmospheres in the potato farming areas of Bangladesh. Antibacterial activity of the isolated probable antagonistic bacteria was testedin vitroagainst the previously identified most common and most virulent soft rot causing bacterial strain Ecc P-138. Only two isolates E-45 and E-65 significantly inhibited thein vitrogrowth of Ecc P-138. Physiological, biochemical, and carbon source utilization tests identified isolate E-65 as a member of the genusBacillusand the isolate E-45 asLactobacillussp. The stronger antagonistic activity against Ecc P-138 was found in E-65in vitroscreening and storage potatoes. E-65 reduced the soft rot infection to 22-week storage potatoes of different varieties by 32.5–62.5% in model experiment, demonstrating its strong potential to be used as an effective biological control agent for the major pectolytic bacteria Ecc. The highest (62.5%) antagonistic effect of E-65 was observed in the Granola and the lowest (32.7%) of that was found in the Cardinal varieties of the Bangladeshi potatoes. The findings suggest that isolate E-65 could be exploited as a biocontrol agent for potato tubers.

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Evaluation of Bacillus subtilis C186 as a potential biological control of sapstain and mould on unseasoned lumber

Canadian Journal of Microbiology ◽

10.1139/m87-192 ◽

1987 ◽

Vol 33 (12) ◽

pp. 1102-1107 ◽

Cited By ~ 23

Author(s):

Keith A. Seifert ◽

Wendy E. Hamilton ◽

Colette Breuil ◽

Maureen Best

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Biological Control Agent ◽

Control Agent ◽

Mould Growth

Bacillus subtilis C186 was evaluated as a potential biological control agent for sapstain and mould growth on unseasoned lumber. The strain produces antibiotics that are fungistatic to many pine-inhabiting fungi. Although pine blocks precolonized with C186 and subsequently inoculated with staining fungi were generally less discoloured than blocks inoculated only with fungi, discolouration was not sufficiently prevented to warrant further consideration of this strain as a biological control agent. The disappointing performance of C186 is traced to its poor colonization of wood.

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Complete genome sequence of Pseudomonas brassicacearum strain L13-6-12, a biological control agent from the rhizosphere of potato

Standards in Genomic Sciences ◽

10.1186/s40793-016-0215-1 ◽

2017 ◽

Vol 12 (1) ◽

Cited By ~ 7

Author(s):

Christin Zachow ◽

Henry Müller ◽

Jana Monk ◽

Gabriele Berg

Keyword(s):

Biological Control ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Biological Control Agent ◽

Control Agent ◽

Pseudomonas Brassicacearum

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Draft Genome Sequence of Bacillus velezensis Strain E68, Isolated from an Oil Battery

Microbiology Resource Announcements ◽

10.1128/mra.00332-20 ◽

2020 ◽

Vol 9 (24) ◽

Author(s):

Nathan Liang ◽

Suha Jabaji

Keyword(s):

Biological Control ◽

Fungal Pathogens ◽

Biological Control Agent ◽

Draft Genome ◽

Gene Clusters ◽

Control Agent ◽

Biosynthetic Gene ◽

Bacillus Velezensis ◽

Biosynthetic Gene Clusters ◽

Content Type

ABSTRACT Bacillus velezensis strain E68 is a biosurfactant-producing bacterium isolated from an oil battery near Chauvin, Alberta, Canada. Strain E68 exhibited antimicrobial activity against fungal pathogens and could potentially serve as a biological control agent. Its genome was sequenced and annotated, revealing the presence of multiple lipopeptide biosynthetic gene clusters.

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Cladobotryum mycophilum as Potential Biocontrol Agent

Agronomy ◽

10.3390/agronomy9120891 ◽

2019 ◽

Vol 9 (12) ◽

pp. 891

Author(s):

Mila Santos ◽

Fernando Diánez ◽

Alejandro Moreno-Gavíra ◽

Brenda Sánchez-Montesinos ◽

Francisco J. Gea

Keyword(s):

Biological Control ◽

Fusarium Oxysporum ◽

Biocontrol Agent ◽

Biological Control Agent ◽

Control Agent ◽

Phytopathogenic Fungi ◽

Antagonistic Activity ◽

Dual Culture ◽

Potential Biocontrol Agent

A study was conducted to explore the efficacy of potential biocontrol agent Cladobotryum mycophilum against different phytopathogenic fungi. The growth rates of 24 isolates of C. mycophilum were determined, and their antagonistic activity was analysed in vitro and in vivo against Botrytis cinerea, Fusarium oxysporum f. sp. radicis-lycopersici, Fusarium oxysporum f.sp. cucumerinum, Fusarium solani, Phytophthora parasitica, Phytophthora capsici, Pythium aphanidermatum and Mycosphaerella melonis. Most isolates grow rapidly, reaching the opposite end of the Petri dish within 72–96 h. Under dual-culture assays, C. mycophilum showed antagonistic activity in vitro against all phytopathogenic fungi tested, with mycelial growth inhibition ranging from 30 to 90% against all the different phytopathogens tested. Similarly, of all the selected isolates, CL60A, CL17A and CL18A significantly (p < 0.05) reduced the disease incidence and severity in the plant assays compared to the controls for the different pathosystems studied. Based on these results, we conclude that C. mycophilum can be considered as a potential biological control agent in agriculture. This is the first study of Cladobotryum mycophilum as a biological control agent for different diseases caused by highly relevant phytopathogens in horticulture.

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