scholarly journals Ecophysiology of different filamentous Alphaproteobacteria in industrial wastewater treatment plants

Microbiology ◽  
2006 ◽  
Vol 152 (10) ◽  
pp. 3003-3012 ◽  
Author(s):  
Caroline Kragelund ◽  
Yunhong Kong ◽  
Jaap van der Waarde ◽  
Karin Thelen ◽  
Dick Eikelboom ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Industrial Wastewater ◽  
Wastewater Treatment Plants ◽  
Control Strategies ◽  
Bacterial Species ◽  
Enzymic Activity ◽  
Specific Gene ◽  
Industrial Wastewater Treatment ◽  
Potential Control ◽  
Species Specific

The ecophysiology of five filamentous species affiliated to the Alphaproteobacteria was investigated in industrial activated sludge systems. The five species, ‘Candidatus Alysiosphaera europaea’, ‘Candidatus Monilibacter batavus’, ‘Candidatus Alysiomicrobium bavaricum’, ‘Candidatus Sphaeronema italicum’ and Meganema perideroedes, are very abundant in industrial wastewater treatment plants and are often involved in bulking incidents. The morphology of these filamentous bacterial species resembled Eikelboom's Nostocoida limicola, or Type 021N, and could only be correctly identified by using fluorescence in situ hybridization (FISH), applying species-specific gene probes. Two physiological groupings of the five species were found using microautoradiography combined with FISH. Group 1 (‘Ca. Monilibacter batavus' and ‘Ca. Sphaeronema italicum’) utilized many short-chained fatty acids (acetate, pyruvate and propionate), whereas Group 2 (‘Ca. Alysiosphaera europaea’, ‘Ca. Alysiomicrobium bavaricum’ and Meganema perideroedes) could also exploit several sugars, amino acids and ethanol. All species had polyhydroxyalkanoate granules present and several of the species had a very large storage capacity. No activity was found under strict anaerobic conditions, while uptake of substrate was observed in the presence of nitrate or nitrite as potential electron acceptor. However, for all species a reduced number of substrates could be consumed under these conditions compared to aerobic conditions. Only a little exo-enzymic activity was found and nearly all species had a hydrophobic cell surface. Based on knowledge of the ecophysiological potential, control strategies are suggested.

Workers' Exposure to Airborne Bacteria and Endotoxins at Industrial Wastewater Treatment Plants

AIHAJ ◽  
1994 ◽  
Vol 55 (11) ◽  
pp. 1055-1060 ◽  
Author(s):  
Sirpa Laitinen ◽  
Juhani Kangas ◽  
Marjut Kotimaa ◽  
Jyrki Liesivuori ◽  
Pertti J. Martikainen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Industrial Wastewater ◽  
Wastewater Treatment Plants ◽  
Airborne Bacteria ◽  
Industrial Wastewater Treatment

Biochar Synthesis for Industrial Wastewater Treatment: A Critical Review

2020 ◽  
Vol 1008 ◽  
pp. 202-212
Author(s):  
Andy G. Kumi ◽  
Mona G. Ibrahim ◽  
Mahmoud Nasr ◽  
Manabu Fujii
Keyword(s):  
Wastewater Treatment ◽  
Industrial Wastewater ◽  
Wastewater Treatment Plants ◽  
Industrial Effluent ◽  
Pollutant Removal ◽  
Environmental Benefits ◽  
Effluent Treatment ◽  
No Production ◽  
Industrial Wastewater Treatment ◽  
Characterization Methods

Excess sludge generated from wastewater treatment plants (WWTPs) can cause negative impacts on human health, water bodies, aquatic plants, and soil quality. However, the produced sludge could be appropriately managed to obtain various economic and environmental benefits. One of the feasible and practical options of sludge management is the synthesize of biochar via oxygen-limited pyrolysis. The use of biochar adsorbent for pollutant removal offers various advantages such as high adsorption capability, low operating and chemical costs, no production of toxins. Hence, this study addresses the applications of sewage sludge-derived biochar for industrial wastewater treatment. The methods of sludge collection, drying, pulverization, and pyrolysis are illustrated. Biochar characterization methods (SEM, EDX, XRD, and FTIR analyses) and mechanisms of the adsorption process are described. The sludge-derived biochar could be used as an adsorptive material for industrial effluent treatment. Recommendations for future studies that could enhance the adsorption capacity of biochar and modified-biochar are given.

Molecular monitoring of bulking sludge in industrial wastewater treatment plants

2002 ◽  
Vol 46 (1-2) ◽  
pp. 551-558 ◽  
Author(s):  
J. van der Waarde ◽  
J. Krooneman ◽  
B. Geurkink ◽  
A. van der Werf ◽  
D. Eikelboom ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Textile Industry ◽  
Industrial Wastewater ◽  
Wastewater Treatment Plants ◽  
In Situ Hybridisation ◽  
Filamentous Bacteria ◽  
Molecular Monitoring ◽  
Industrial Wastewater Treatment ◽  
Operational Conditions ◽  
Filamentous Microorganisms

Fluorescent In Situ Hybridisation (FISH) was used to monitor the presence of filamentous microorganisms in industrial wastewater treatment plants (WWTPs). Monitoring with a restricted set of FISH probes in WWTPs from potato industry showed growth and decline of Thiothrix populations that could be linked to operational procedures. In a follow up project new FISH probes were developed for filamentous bacteria in industrial WWTPs and 70 WWTPs were analysed for presence of these filaments. Several newly described species of filamentous bacteria appear to be common and dominant in industrial WWTPs. Monitoring of a WWTP from textile industry showed growth and decline of one of these organisms when operational conditions in the plant were varied. The present paper demonstrates that bulking sludge in industrial wastewater treatment plants can effectively be monitored using a combination of standard chemical analyses and the FISH technique.

scholarly journals Quantification of Hyphomicrobium Populations in Activated Sludge from an Industrial Wastewater Treatment System as Determined by 16S rRNA Analysis

2000 ◽  
Vol 66 (3) ◽  
pp. 1167-1174 ◽  
Author(s):  
A. C. Layton ◽  
P. N. Karanth ◽  
C. A. Lajoie ◽  
A. J. Meyers ◽  
I. R. Gregory ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
16S Rrna ◽  
Activated Sludge ◽  
16S Rdna ◽  
Industrial Wastewater ◽  
Bacterial Species ◽  
Treatment Plant ◽  
Rrna Gene ◽  
16S Rrna Analysis ◽  
Industrial Wastewater Treatment

ABSTRACT The bacterial community structure of the activated sludge from a 25 million-gal-per-day industrial wastewater treatment plant was investigated using rRNA analysis. 16S ribosomal DNA (rDNA) libraries were created from three sludge samples taken on different dates. Partial rRNA gene sequences were obtained for 46 rDNA clones, and nearly complete 16S rRNA sequences were obtained for 18 clones. Seventeen of these clones were members of the beta subdivision, and their sequences showed high homology to sequences of known bacterial species as well as published 16S rDNA sequences from other activated sludge sources. Sixteen clones belonged to the alpha subdivision, 7 of which showed similarity to Hyphomicrobium species. This cluster was chosen for further studies due to earlier work onHyphomicrobium sp. strain M3 isolated from this treatment plant. A nearly full-length 16S rDNA sequence was obtained fromHyphomicrobium sp. strain M3. Phylogenetic analysis revealed that Hyphomicrobium sp. strain M3 was 99% similar to Hyphomicrobium denitrificans DSM 1869T inHyphomicrobium cluster II. Three of the cloned sequences from the activated sludge samples also grouped with those ofHyphomicrobium cluster II, with a 96% sequence similarity to that of Hyphomicrobium sp. strain M3. The other four cloned sequences from the activated sludge sample were more closely related to those of the Hyphomicrobium cluster I organisms (95 to 97% similarity). Whole-cell fluorescence hybridization of microorganisms in the activated sludge with genus-specificHyphomicrobium probe S-G-Hypho-1241-a-A-19 enhanced the visualization of Hyphomicrobium and revealed thatHyphomicrobium appears to be abundant both on the outside of flocs and within the floc structure. Dot blot hybridization of activated sludge samples from 1995 with probes designed forHyphomicrobium cluster I and Hyphomicrobiumcluster II indicated that Hyphomicrobium cluster II-positive 16S rRNA dominated over Hyphomicrobium cluster I-positive 16S rRNA by 3- to 12-fold. Hyphomicrobium 16S rRNA comprised approximately 5% of the 16S rRNA in the activated sludge.

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