scholarly journals Identification of protein secretion systems in bacterial genomes

2015 ◽  
Author(s):  
Sophie S Abby ◽  
Jean Cury ◽  
Julien Guglielmini ◽  
Bertrand Néron ◽  
Marie Touchon ◽  
...  
Keyword(s):  
Protein Secretion ◽  
Single Copy ◽  
Secretion Systems ◽  
Bacterial Genomes ◽  
Accurate Identification ◽  
Outer Membranes ◽  
Type Iv ◽  
Genetic Organisation ◽  
Environmental Bacteria ◽  
Protein Secretion Systems

Bacteria with two cell membranes (diderms) have evolved complex systems for protein secretion. These systems were extensively studied in some model bacteria, but the characterisation of their diversity has lagged behind due to lack of standard annotation tools. We built models for accurate identification of protein secretion systems and related appendages in bacteria with LPS-containing outer membranes. They can be used with MacSyFinder (standalone program) or online (http://mobyle.pasteur.fr/cgi-bin/portal.py#forms::txsscan). They include protein profiles and information on the system's composition and genetic organisation. They can be used to search for T1SS-T6SS, T9SS, and accessorily for flagella, Type IV and Tad pili. We identified ~10,000 systems in bacterial genomes, where T1SS and T5SS were by far the most abundant and widespread. The recently described T6SSiii and T9SS were restricted to Bacteroidetes, and T6SSii to Francisella. T2SS, T3SS, and T4SS were frequently encoded in single-copy in one locus, whereas most T1SS were encoded in two loci. The secretion systems of diderm Firmicutes were similar to those found in other diderms. Novel systems may remain to be discovered, since some clades of environmental bacteria lacked all known protein secretion systems. Our models can be fully customized, which should facilitate the identification of novel systems.

scholarly journals Identification of protein secretion systems in bacterial genomes

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Sophie S. Abby ◽  
Jean Cury ◽  
Julien Guglielmini ◽  
Bertrand Néron ◽  
Marie Touchon ◽  
...  
Keyword(s):  
Protein Secretion ◽  
Secretion Systems ◽  
Bacterial Genomes ◽  
Protein Secretion Systems

Genome Update: Protein secretion systems in 225 bacterial genomes

Microbiology ◽  
2005 ◽  
Vol 151 (4) ◽  
pp. 1013-1016 ◽  
Author(s):  
Tim T. Binnewies ◽  
Jannick D. Bendtsen ◽  
Peter F. Hallin ◽  
Natasja Nielsen ◽  
Trudy M. Wassenaar ◽  
...  
Keyword(s):  
Protein Secretion ◽  
Secretion Systems ◽  
Bacterial Genomes ◽  
Protein Secretion Systems

Protein Secretion Systems in Microbial and Mammalian Cells

2020 ◽  
pp. 67-94
Author(s):  
Donald T. Moir ◽  
Jen-I Mao
Keyword(s):  
Protein Secretion ◽  
Mammalian Cells ◽  
Secretion Systems ◽  
Protein Secretion Systems

News and views on protein secretion systems

2015 ◽  
pp. 77-108 ◽  
Author(s):  
Alain Filloux ◽  
Agnes Sagfors
Keyword(s):  
Protein Secretion ◽  
Secretion Systems ◽  
Protein Secretion Systems

scholarly journals T4SP Database 2.0: An Improved Database for Type IV Secretion Systems in Bacterial Genomes with New Online Analysis Tools

2016 ◽  
Vol 2016 ◽  
pp. 1-4 ◽  
Author(s):  
Na Han ◽  
Weiwen Yu ◽  
Yujun Qiang ◽  
Wen Zhang
Keyword(s):  
Recipient Cell ◽  
Bacterial Species ◽  
Genetic Material ◽  
Type Iv Secretion ◽  
Bacterial Strains ◽  
Secretion Systems ◽  
Bacterial Genomes ◽  
Type Iv ◽  
Type Iv Secretion Systems ◽  
User Friendly

Type IV secretion system (T4SS) can mediate the passage of macromolecules across cellular membranes and is essential for virulent and genetic material exchange among bacterial species. The Type IV Secretion Project 2.0 (T4SP 2.0) database is an improved and extended version of the platform released in 2013 aimed at assisting with the detection of Type IV secretion systems (T4SS) in bacterial genomes. This advanced version provides users with web server tools for detecting the existence and variations of T4SS genes online. The new interface for the genome browser provides a user-friendly access to the most complete and accurate resource of T4SS gene information (e.g., gene number, name, type, position, sequence, related articles, and quick links to other webs). Currently, this online database includes T4SS information of 5239 bacterial strains.Conclusions. T4SS is one of the most versatile secretion systems necessary for the virulence and survival of bacteria and the secretion of protein and/or DNA substrates from a donor to a recipient cell. This database on virB/D genes of the T4SS system will help scientists worldwide to improve their knowledge on secretion systems and also identify potential pathogenic mechanisms of various microbial species.

Super-resolution imaging of bacterial pathogens and visualization of their secreted effectors

2020 ◽  
Author(s):  
Moirangthem Kiran Singh ◽  
Linda J Kenney
Keyword(s):  
Virulence Factors ◽  
Protein Secretion ◽  
Fluorescent Probes ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Bacterial Pathogenesis ◽  
Secretion Systems ◽  
Complex Protein ◽  
Resolution Imaging ◽  
Protein Secretion Systems

ABSTRACT Recent advances in super-resolution imaging techniques, together with new fluorescent probes have enhanced our understanding of bacterial pathogenesis and their interplay within the host. In this review, we provide an overview of what these techniques have taught us about the bacterial lifestyle, the nucleoid organization, its complex protein secretion systems, as well as the secreted virulence factors.

scholarly journals Comparison of ATPase-Encoding Type III Secretion System hrcN Genes in Biocontrol Fluorescent Pseudomonads and in Phytopathogenic Proteobacteria

2004 ◽  
Vol 70 (9) ◽  
pp. 5119-5131 ◽  
Author(s):  
Fabio Rezzonico ◽  
Geneviève Défago ◽  
Yvan Moënne-Loccoz
Keyword(s):  
Pseudomonas Syringae ◽  
Protein Secretion ◽  
Type Iii Secretion ◽  
Fluorescent Pseudomonads ◽  
Secretion Systems ◽  
Associated Bacteria ◽  
Type Iii ◽  
Encoding Gene ◽  
Type Iii Protein Secretion ◽  
Protein Secretion Systems

ABSTRACT Type III protein secretion systems play a key role in the virulence of many pathogenic proteobacteria, but they also occur in nonpathogenic, plant-associated bacteria. Certain type III protein secretion genes (e.g., hrcC) have been found in Pseudomonas sp. strain SBW25 (and other biocontrol pseudomonads), but other type III protein secretion genes, such as the ATPase-encoding gene hrcN, have not been found. Using both colony hybridization and a PCR approach, we show here that hrcN is nevertheless present in many biocontrol fluorescent pseudomonads. The phylogeny of biocontrol Pseudomonas strains based on partial hrcN sequences was largely congruent with the phylogenies derived from analyses of rrs (encoding 16S rRNA) and, to a lesser extent, biocontrol genes, such as phlD (for 2,4-diacetylphloroglucinol production) and hcnBC (for HCN production). Most biocontrol pseudomonads clustered separately from phytopathogenic proteobacteria, including pathogenic pseudomonads, in the hrcN tree. The exception was strain KD, which clustered with phytopathogenic pseudomonads, such as Pseudomonas syringae, suggesting that hrcN was acquired from the latter species. Indeed, strain KD (unlike strain SBW25) displayed the same organization of the hrpJ operon, which contains hrcN, as P. syringae. These results indicate that the occurrence of hrcN in most biocontrol pseudomonads is not the result of recent horizontal gene transfer from phytopathogenic bacteria, although such transfer might have occurred for a minority of biocontrol strains.

Sign in / Sign up

Export Citation Format

Share Document