scholarly journals The collective influence of 1, 25-dihydroxyvitamin D3with physiological fluid shear stress on osteoblasts

2017 ◽  
Author(s):  
Yan Li ◽  
Jiafeng Yuan ◽  
Qianwen Wang ◽  
Lijie Sun ◽  
Yunying Sha ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Mechanical Stimuli ◽  
Fluid Shear ◽  
Dihydroxyvitamin D ◽  
Physiological Fluid ◽  
Flow Shear Stress ◽  
System 1 ◽  
Collective Influence

Abstract1, 25-dihydroxyvitamin D3(1, 25 (OH)2D3) and mechanical stimuli in physiological environment play an important role in the pathogenesis of osteoporosis. The effects of 1, 25-dihydroxyvitamin D3alone and mechanical stimuli alone on osteoblasts have been widely investigated. This study reports the collective influences of 1, 25-dihydroxyvitamin D3and flow shear stress (FSS) on biological functions of osteoblasts. 1, 25 (OH)2D3were constructed in various kinds of concentration (0, 1, 10, 100 nmmol/L), while physiological fluid shear stress (12 dynes/cm2) were produced by using a parallel-plate fluid flow system. 1, 25 (OH)2D3affects the responses of ROBs to FSS, including the inhibition of NO releases and cell proliferation as well as the promotion of PGE2releases and cell differentiation. These findings provide a possible mechanism by which 1, 25(OH)2D3influences osteoblasts responses to FSS and may provide guidance for the selection of 1, 25(OH)2D3concentration and mechanical loading in order toin vitroproduce functional bone tissues.

scholarly journals The collective influence of 1, 25-dihydroxyvitamin D 3 with physiological fluid shear stress on osteoblasts

Steroids ◽  
2018 ◽  
Vol 129 ◽  
pp. 9-16
Author(s):  
Yan Li ◽  
Jiafeng Yuan ◽  
Qianwen Wang ◽  
Lijie Sun ◽  
Yunying Sha ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Fluid Shear ◽  
Dihydroxyvitamin D ◽  
Physiological Fluid ◽  
Collective Influence

scholarly journals Turbulent fluid shear stress induces vascular endothelial cell turnover in vitro.

1986 ◽  
Vol 83 (7) ◽  
pp. 2114-2117 ◽  
Author(s):  
P. F. Davies ◽  
A. Remuzzi ◽  
E. J. Gordon ◽  
C. F. Dewey ◽  
M. A. Gimbrone
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Fluid Shear Stress ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Cell Turnover ◽  
Fluid Shear ◽  
Turbulent Fluid

scholarly journals ShearFAST: a user-friendly in vitro toolset for high throughput, inexpensive fluid shear stress experiments.

2020 ◽  
Author(s):  
Thomas Brendan Smith ◽  
Alessandro Marco De Nunzio ◽  
Kamlesh Patel ◽  
Haydn Munford ◽  
Tabeer Alam ◽  
...  
Keyword(s):  
Shear Stress ◽  
High Throughput ◽  
Fluid Shear Stress ◽  
Tracking System ◽  
Frequency Measurement ◽  
Camera Motion ◽  
Fluid Shear ◽  
Mean Frequency

Fluid shear stress is a key modulator of cellular physiology in vitro and in vivo, but its effects are under-investigated due to requirements for complicated induction methods. Herein we report the validation of ShearFAST; a smartphone application that measures the rocking profile on a standard laboratory cell rocker and calculates the resulting shear stress arising in tissue culture plates. The accuracy with which this novel approach measured rocking profiles was validated against a graphical analysis, and also against measures reported by an 8-camera motion tracking system. ShearFASTs angle assessments correlated well with both analyses (r ≥0.99, p ≤0.001) with no significant differences in pitch detected across the range of rocking angles tested. Rocking frequency assessment by ShearFAST also correlated well when compared to the two independent validatory techniques (r ≥0.99, p ≤0.0001), with excellent reproducibility between ShearFAST and video analysis (mean frequency measurement difference of 0.006 ± 0.005Hz) and motion capture analysis (mean frequency measurement difference of 0.008 ± 0.012Hz). These data make the ShearFAST assisted cell rocker model make it an attractive approach for economical, high throughput fluid shear stress experiments. Proof of concept data presented reveals a protective effect of low-level shear stress on renal proximal tubule cells submitted to simulations of pretransplant storage.

Physiological fluid shear stress causes downregulation of endothelin-1 mRNA in bovine aortic endothelium

1992 ◽  
Vol 263 (2) ◽  
pp. C389-C396 ◽  
Author(s):  
A. Malek ◽  
S. Izumo
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Fluid Velocity ◽  
Low Frequency ◽  
Turbulent Shear ◽  
Specific Response ◽  
Dependent Manner ◽  
Fluid Shear ◽  
Endothelin 1

We report here that the level of endothelin-1 (ET-1) mRNA from bovine aortic endothelial cells grown in vitro is rapidly (within 1 h of exposure) and significantly (fivefold) decreased in response to fluid shear stress of physiological magnitude. The downregulation of ET-1 mRNA occurs in a dose-dependent manner that exhibits saturation above 15 dyn/cm2. The decrease is complete prior to detectable changes in endothelial cell shape and is maintained throughout and following alignment in the direction of blood flow. Peptide levels of ET-1 secreted into the media are also reduced in response to fluid shear stress. Cyclical stretch experiments demonstrated no changes in ET-1 mRNA, while increasing media viscosity with dextran showed that the downregulation is a specific response to shear stress and not to fluid velocity. Although both pulsatile and turbulent shear stress of equal time-average magnitude elicited the same decrease in ET-1 mRNA as steady laminar shear (15 dyn/cm2), low-frequency reversing shear stress did not result in any change. These results show that the magnitude as well as the dynamic character of fluid shear stress can modulate expression of ET-1 in vascular endothelium.

Nanoparticle localization in blood vessels: dependence on fluid shear stress, flow disturbances, and flow-induced changes in endothelial physiology

Nanoscale ◽  
2018 ◽  
Vol 10 (32) ◽  
pp. 15249-15261 ◽  
Author(s):  
M. Juliana Gomez-Garcia ◽  
Amber L. Doiron ◽  
Robyn R. M. Steele ◽  
Hagar I. Labouta ◽  
Bahareh Vafadar ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Fluid Shear ◽  
Nanoparticle Distribution ◽  
Flow Models ◽  
Flow Disturbances ◽  
Induced Changes ◽  
Stress Flow

Hemodynamic factors drive nanoparticle distribution in vivo and in vitro in cell-based flow models.

scholarly journals In vitro generated extracellular matrix and fluid shear stress synergistically enhance 3D osteoblastic differentiation

2006 ◽  
Vol 103 (8) ◽  
pp. 2488-2493 ◽  
Author(s):  
N. Datta ◽  
Q. P. Pham ◽  
U. Sharma ◽  
V. I. Sikavitsas ◽  
J. A. Jansen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Shear Stress ◽  
Fluid Shear Stress ◽  
Osteoblastic Differentiation ◽  
Fluid Shear

scholarly journals Effect of fluid shear stress on in vitro cultured ureteric bud cells

2018 ◽  
Vol 12 (4) ◽  
pp. 044107 ◽  
Author(s):  
Hiroshi Kimura ◽  
Masaki Nishikawa ◽  
Naomi Yanagawa ◽  
Hiroko Nakamura ◽  
Shunsuke Miyamoto ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Ureteric Bud ◽  
Fluid Shear
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