Impairment of zebrafish reproduction upon exposure to melengestrol acetate

AbstractSynthetic progestins contamination is common in the aquatic ecosystem, which may lead to serious health problem on aquatic animals. Melengestrol acetate (MGA) has been detected in the aquatic environment; however, its potential effects on fish reproduction are largely unclear. Here, we aimed to investigate the endocrine disruption and impact of MGA on zebrafish reproduction. Six-month old reproductive zebrafish were exposed to four nominal concentrations of MGA (1,10, 100 and 200 ng/L) for 15 days. Treatment with MGA reduced the egg production with a significant decrease at 200 ng/L. The circulating concentrations of estradiol and testosterone in female zebrafish or 11-keto testosterone in male zebrafish were significantly diminished compared to the non-exposed control fish. The early embryonic development or hatching rates were unaffected during the MGA exposure. Our results indicated that MGA was a potent endocrine disruptor in fish and the fish reproduction could be impaired even during a short-term exposure to MGA.

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Endocrine disruption and oxidative stress in larvae of Chironomus dilutus following short-term exposure to fresh or aged oil sands process-affected water

Aquatic Toxicology ◽

10.1016/j.aquatox.2013.09.003 ◽

2013 ◽

Vol 142-143 ◽

pp. 414-421 ◽

Cited By ~ 40

Author(s):

S.B. Wiseman ◽

J.C. Anderson ◽

K. Liber ◽

J.P. Giesy

Keyword(s):

Oxidative Stress ◽

Endocrine Disruption ◽

Oil Sands ◽

Short Term ◽

Short Term Exposure ◽

And Oxidative Stress ◽

Chironomus Dilutus

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Short-term exposure to an endocrine disruptor affects behavioural consistency in male threespine stickleback

Aquatic Toxicology ◽

10.1016/j.aquatox.2011.09.010 ◽

2011 ◽

Vol 105 (3-4) ◽

pp. 681-687 ◽

Cited By ~ 11

Author(s):

Teresa L. Dzieweczynski

Keyword(s):

Endocrine Disruptor ◽

Threespine Stickleback ◽

Short Term ◽

Behavioural Consistency ◽

Short Term Exposure

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Effects of progesterone on the reproductive physiology in zebrafish

10.1101/147280 ◽

2017 ◽

Author(s):

Chunyun Zhong ◽

Kewen Xiong ◽

Xin Wang

Keyword(s):

Endocrine System ◽

Aquatic Organisms ◽

Fish Reproduction ◽

Health Issues ◽

Adult Zebrafish ◽

Female Fish ◽

Short Term ◽

Male Fish ◽

Adverse Health Effects ◽

Short Term Exposure

AbstractRecent studies have investigated that the synthetic progestins may lead to health issues to the aquatic organisms. Progesterone is a steroidal progestin and has been used as a contraceptive drug, which is detected in the aquatic ecosystem. However, the potential effects of progesterone on the fish reproduction are largely unclear. Here, we tested the effects of progesterone on the fish reproductive and endocrine systems. Adult zebrafish were exposed to progesterone for 10 days at environmental concentrations. The production of eggs was reduced in the exposed fish, and the circulating concentrations of estradiol (E2) and testosterone (T) in female fish or 11-keto testosterone (11-KT) in male fish were significantly diminished. Our results suggested that progesterone may cause adverse health effects on fish by disrupting the endocrine system, and short-term exposure to progesterone could overt affect the fish reproduction.

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The Effect of Dimethyl Sulfoxide on In Vitro Platelet Function

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648027 ◽

1976 ◽

Vol 36 (01) ◽

pp. 221-229 ◽

Cited By ~ 16

Author(s):

Charles A. Schiffer ◽

Caroline L. Whitaker ◽

Morton Schmukler ◽

Joseph Aisner ◽

Steven L. Hilbert

Keyword(s):

Platelet Count ◽

Platelet Aggregation ◽

Dimethyl Sulfoxide ◽

Platelet Function ◽

Platelet Volume ◽

Short Term ◽

Platelet Factor ◽

Short Term Exposure ◽

Structural Abnormalities

SummaryAlthough dimethyl sulfoxide (DMSO) has been used extensively as a cryopreservative for platelets there are few studies dealing with the effect of DMSO on platelet function. Using techniques similar to those employed in platelet cryopreservation platelets were incubated with final concentrations of 2-10% DMSO at 25° C. After exposure to 5 and 10% DMSO platelets remained discoid and electron micrographs revealed no structural abnormalities. There was no significant change in platelet count. In terms of injury to platelet membranes, there was no increased availability of platelet factor-3 or leakage of nucleotides, 5 hydroxytryptamine (5HT) or glycosidases with final DMSO concentrations of 2.5, 5 and 10% DMSO. Thrombin stimulated nucleotide and 5HT release was reduced by 10% DMSO. Impairment of thrombin induced glycosidase release was noted at lower DMSO concentrations and was dose related. Similarly, aggregation to ADP was progressively impaired at DMSO concentrations from 1-5% and was dose related. After the platelets exposed to DMSO were washed, however, aggregation and release returned to control values. Platelet aggregation by epinephrine was also inhibited by DMSO and this could not be corrected by washing the platelets. DMSO-plasma solutions are hypertonic but only minimal increases in platelet volume (at 10% DMSO) could be detected. Shrinkage of platelets was seen with hypertonic solutions of sodium chloride or sucrose suggesting that the rapid transmembrane passage of DMSO prevented significant shifts of water. These studies demonstrate that there are minimal irreversible alterations in in vitro platelet function after short-term exposure to DMSO.

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Responses in a Fathead Minnow (Pimephales promelas) Lifecycle Test and in Wild White Sucker (Catostomus commersoni) Exposed to a Canadian Bleached Kraft Mill Effluent

Water Quality Research Journal ◽

10.2166/wqrj.2010.022 ◽

2010 ◽

Vol 45 (2) ◽

pp. 187-200 ◽

Cited By ~ 9

Author(s):

Joanne L. Parrott ◽

L. Mark Hewitt ◽

Tibor G. Kovacs ◽

Deborah L. MacLatchy ◽

Pierre H. Martel ◽

...

Keyword(s):

Egg Production ◽

Fathead Minnow ◽

Pimephales Promelas ◽

Fish Reproduction ◽

White Sucker ◽

Catostomus Commersoni ◽

Liver Size ◽

Paper Mill Effluent ◽

Kraft Mill Effluent ◽

Kraft Mill

Abstract To evaluate currently available bioassays for their use in investigating the causes of pulp and paper mill effluent effects on fish reproduction, the responses of wild white sucker (Catostomus commersoni) collected from the receiving environment at the bleached kraft mill at La Tuque, Quebec, were compared with responses of fathead minnow (Pimephales promelas) exposed to effluent in a laboratory lifecycle test. White sucker collected at effluent exposed sites had increased liver size but none of the reproductive effects that had been documented in earlier field studies at this site. Exposure to 1, 3, 10, 30, and 100% bleached kraft mill effluent (BKME) in the lab led to significantly decreased length, but increased weight and liver size in male fathead minnow. Female length was also decreased and liver size was increased at high effluent exposures. Most effluent concentrations (1 to 30%) significantly increased egg production compared with controls. The fathead minnow lifecycle assay mirrored the effects seen in wild fish captured downstream of the BKME discharge. These results will be used to select short-term fish tests for investigating the causes of and solutions to the effects of mill effluents on fish reproduction.

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The Benefits of Biotreatment for Reducing the Effects of Pulp and Paper Mill Effluents on Fish Reproduction in Laboratory Tests

Water Quality Research Journal ◽

10.2166/wqrj.2008.019 ◽

2008 ◽

Vol 43 (2-3) ◽

pp. 161-171 ◽

Cited By ~ 4

Author(s):

Pierre Martel ◽

Tibor Kovacs ◽

Virginie Bérubé

Keyword(s):

Laboratory Tests ◽

Egg Production ◽

Pimephales Promelas ◽

Paper Mill ◽

Pulp And Paper ◽

Reproductive Capacity ◽

Fish Reproduction ◽

Pulp And Paper Mill ◽

Paper Mill Effluents ◽

Gonad Size

Abstract Pulp and paper mill effluents have been reported to cause changes in reproductive indicators of fish in laboratory and field studies. These changes include reduced egg production and gonad size, and altered hormone levels and expression of secondary sex characteristics. We examined the performance of biotreatment plants for their potential in abating effects of pulp and paper mill effluents on fish reproduction under laboratory conditions. A bleached kraft mill effluent (BKME) treated in an aerated lagoon and a thermomechanical pulp mill effluent (TMPE) treated by aerobic sludge in a sequential batch reactor were selected for study. Mature fathead minnows (Pimephales promelas) were exposed to effluents before and after biotreatment under continuous renewal conditions for 21 days. Egg production was monitored daily, while morphometric parameters (length, weight, gonad size), secondary sexual characteristics, and steroid hormone and vitellogenin levels were measured at the end of the effluent exposure. The effluent from both mills before biotreatment impaired the reproductive capacity of minnows (egg production) at concentrations of 10 and 20% vol/vol, but not at 2% vol/vol. Exposure to biotreated effluents from both mills at concentrations of 2, 10, 20, and 40% vol/vol caused no significant differences in overall reproductive capacity of minnows as compared with controls. These results indicate that biotreatment can significantly improve the quality of a BKME and an effluent from a TMP mill with respect to the reproductive capacity of fish as determined in laboratory tests.

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