Rapid non- invasive detection of Influenza- A- infection by multicapillary column coupled ion mobility spectrometry

Infectious pathogens are a global issue. Global air travelling offers an easy and fast opportunity not only for people but also for infectious diseases to spread around the world within a few days. Also, large public events facilitate increasing infection numbers. Therefore, a rapid on-site screening for infected people is urgently needed. Due to the small size and easy handling, the ion mobility spectrometry coupled with a multicapillary column (MCC-IMS) is a very promising, sensitive method for the on-site identification of infectious pathogens based on scents, representing volatile organic compounds (VOCs). The purpose of this study was to prospectively assess whether identification of Influenza-A- infection based on VOCs by MCC-IMS is possible in breath. Nasal breath was investigated in 24 consecutive persons with and without Influenza-A-infection by MCC-IMS. In 14 Influenza-A-infected patients, infection was proven by PCR of nasopharyngeal swabs. Four healthy staff members and six patients with negative PCR result served as controls. For picking up relevant VOCs in MCC-IMS spectra, software based on cluster analysis followed by multivariate statistical analysis was applied. With only four VOCs canonical discriminant analysis was able to distinguish Influenza-A-infected patients from not infected with 100% sensitivity and 100% specificity. This present proof-of-concept- study yields encouraging results showing a rapid diagnosis of viral infections in nasal breath within 5 minutes by MCC-IMS. The next step is to validate the results with a greater number of patients with Influenza-A-infection as well as other viral diseases, especially COVID-19. Registration number at ClinicalTrials.gov NCT04282135.

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Rapid non-invasive detection of Influenza-A-infection by multicapillary column coupled ion mobility spectrometry

Journal of Breath Research ◽

10.1088/1752-7163/abb762 ◽

2020 ◽

Vol 15 (1) ◽

pp. 011001

Author(s):

Claus Steppert ◽

Isabel Steppert ◽

Thomas Bollinger ◽

William Sterlacci

Keyword(s):

Ion Mobility Spectrometry ◽

Ion Mobility ◽

Influenza A ◽

Non Invasive ◽

Multicapillary Column

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Ion mobility spectrometry combined with multivariate statistical analysis: revealing the effects of a drug candidate for Alzheimer’s disease on Aβ1-40 peptide early assembly

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-019-02030-7 ◽

2019 ◽

Vol 411 (24) ◽

pp. 6353-6363

Author(s):

Serena Lazzaro ◽

Nina Ogrinc ◽

Lieke Lamont ◽

Graziella Vecchio ◽

Giuseppe Pappalardo ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Statistical Analysis ◽

Ion Mobility Spectrometry ◽

Ion Mobility ◽

Multivariate Statistical Analysis ◽

Drug Candidate ◽

Multivariate Statistical

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Static headspace-multicapillary column with gas chromatography coupled to ion mobility spectrometry as a simple approach for the discrimination of crude and processed traditional Chinese medicines

Journal of Separation Science ◽

10.1002/jssc.201400627 ◽

2014 ◽

Vol 37 (21) ◽

pp. 3090-3093 ◽

Cited By ~ 6

Author(s):

Gang Cao ◽

Qiyang Shou ◽

Qinglin Li ◽

Jianping Jiang ◽

Xiaocheng Chen

Keyword(s):

Gas Chromatography ◽

Ion Mobility Spectrometry ◽

Ion Mobility ◽

Simple Approach ◽

Traditional Chinese Medicines ◽

Static Headspace ◽

Chinese Medicines ◽

Multicapillary Column

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On-line headspace-multicapillary column-ion mobility spectrometry hyphenation as a tool for the determination of off-flavours in foods

Journal of Chromatography A ◽

10.1016/j.chroma.2014.01.062 ◽

2014 ◽

Vol 1333 ◽

pp. 99-105 ◽

Cited By ~ 15

Author(s):

Isabel Márquez-Sillero ◽

Soledad Cárdenas ◽

Stefanie Sielemann ◽

Miguel Valcárcel

Keyword(s):

Ion Mobility Spectrometry ◽

Ion Mobility ◽

On Line ◽

Multicapillary Column

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Detection of malignant pleural mesothelioma in exhaled breath by multicapillary column/ion mobility spectrometry (MCC/IMS)

Journal of Breath Research ◽

10.1088/1752-7155/10/4/046001 ◽

2016 ◽

Vol 10 (4) ◽

pp. 046001 ◽

Cited By ~ 10

Author(s):

Kevin Lamote ◽

Matthijs Vynck ◽

Joris Van Cleemput ◽

Olivier Thas ◽

Kristiaan Nackaerts ◽

...

Keyword(s):

Malignant Pleural Mesothelioma ◽

Ion Mobility Spectrometry ◽

Ion Mobility ◽

Pleural Mesothelioma ◽

Exhaled Breath ◽

Multicapillary Column

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Evaluation and application of static headspace–multicapillary column-gas chromatography–ion mobility spectrometry for complex sample analysis

Journal of Chromatography A ◽

10.1016/j.chroma.2014.02.047 ◽

2014 ◽

Vol 1338 ◽

pp. 136-148 ◽

Cited By ~ 24

Author(s):

Chamila J. Denawaka ◽

Ian A. Fowlis ◽

John R. Dean

Keyword(s):

Gas Chromatography ◽

Ion Mobility Spectrometry ◽

Ion Mobility ◽

Static Headspace ◽

Sample Analysis ◽

Complex Sample ◽

Multicapillary Column

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Rapid detection of SARS-CoV-2 infection by multicapillary column coupled ion mobility spectrometry (MCC-IMS) of breath. A proof of concept study

10.21203/rs.3.rs-70139/v1 ◽

2020 ◽

Author(s):

Claus Steppert ◽

Isabel Steppert ◽

William Sterlacci ◽

Thomas Bollinger

Keyword(s):

Ion Mobility ◽

Influenza A ◽

Viral Disease ◽

Pcr Analysis ◽

Proof Of Concept ◽

Influenza Epidemic ◽

Rt Pcr ◽

Respective Group ◽

Non Invasive ◽

Multicapillary Column

Abstract There is an urgent need for screening of patients having a communicable viral disease to cut infection chains. We could recently demonstrate that MCC-IMS of breath is able to identify Influenza-A infected patients. With decreasing Influenza epidemic and upcoming SARS-CoV-2 infections we went on and also analysed patients with suspected SARS-CoV-2 infections.75 patients, 34m, 41f, aged 64.4 ± 15.4 years, 14 positive for Influenza-A, 16 positive for SARS-CoV-2, the remaining 44 patients were used as controls. In one patient RT-PCR was highly suspicious of SARS-CoV-2 but initially inconclusiveBesides RT-PCR analysis of nasopharyngeal swabs all patients underwent MCC-IMS analysis of breath. There was no difference in gender or age according to the groups.97.3% of the patients could be correctly classified to the respective group by discriminant analysis. Even the inconclusive patient could be mapped to the SARS-CoV-2 group applying the discrimination function.ConclusionMCC-IMS is able to detect SARS-CoV-2 infection and Influenza-A infection in breath. As this method provides exact, fast non-invasive diagnosis it should be further developed for screening of communicable viral diseases.Trial registrationClinicalTrial.gov, NCT04282135 Registered 20 February 2020 - Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT04282135?term=IMS&draw=2&rank=1

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MICROBIOLOGICAL DIAGNOSIS AND IMPORTANCE OF INFECTIONS CAUSED BY INFLENZAE – А VIRUS

Knowledge International Journal ◽

10.35120/kij31041057c ◽

2019 ◽

Vol 31 (4) ◽

pp. 1057-1061

Author(s):

Zivadinka Cvetanovska ◽

Vaso Taleski

Keyword(s):

Influenza A ◽

Viral Infections ◽

Respiratory Infections ◽

Great Influence ◽

Influenza Viruses ◽

Influenza B ◽

Number Of Patients ◽

Viral Respiratory Infections ◽

Subtype A

Influenza is one of the commonest acute viral respiratory infections with a great potential for spreading as an epidemic or pandemic appearance. Until 2009 relevant data about types and subtypes of influenza viruses circulated in Republic of Macedonia, did not exist. Since pandemic in 2009, molecular method RT-PCR was introduced real time detection of types and subtypes of influenza viruses, which enabled continuously and accurate follow up. Flu differ in types and subtypes presence in each new season, with great influence on number of patients and deaths caused by influenza viral infections. In season 2009/2010 - type Influenza A dominated, subtype A (H1N1) pdm. Total number of 54.343 cases were registered and 30 deaths. In season 2010/2011 – co-circulated types of Influenza A and Influenza B, with small domination of subtype A (H1N1) pdm. Total number of 27.635 cases were registered and 17 deaths. In season 2011/2012 - dominant was type Influenza A, subtype A (H3N2). Total number of 9.732 cases were registered and only one case of death. In season 2012/2013 - co-circulated types of Influenza A and Influenza B, with domination of subtype A (H1N1) pdm. Total number of 24.524 were registered, no deaths. In season 2013/2014 - co-circulated types of Influenza A and Influenza B, with domination of subtype A (H3N2). Total number of 29.074 were registered and 12 deaths. In season 2014/2015 - dominant was type Influenza B, and also Influenza A subtype A(H3N2) circulated. Total number of reported cases was 33.228, no deaths. In season 2015/2016 - Influenza A, subtype A(H1N1) pdm was dominant. During same period, type Influenza B, subtype Victoria was detected as well. Total number of reported cases was 29.094 and 2 deaths. In season 2016/2017 - type Influenza A, subtype A/H3, was dominant. Total number of reported cases was 35.079 and 2 deaths. In season 2017/2018 година – simultaneously circulation of types Influenza А and Influenza B, with domination of lineage B/Yamagata. Total number of 23.954 cases were registered, no deaths. In season 2018/2019 - highest number of cases were caused by type Influenza A subtype A(H1) pdm, in co-circulation with Influenza А(H3). Total number of reported cases was 21.404 and 29 deaths, that present the highest number of deaths in correlation with number of diseased.

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