Rapid non-invasive detection of Influenza-A-infection by multicapillary column coupled ion mobility spectrometry

Infectious pathogens are a global issue. Global air travelling offers an easy and fast opportunity not only for people but also for infectious diseases to spread around the world within a few days. Also, large public events facilitate increasing infection numbers. Therefore, a rapid on-site screening for infected people is urgently needed. Due to the small size and easy handling, the ion mobility spectrometry coupled with a multicapillary column (MCC-IMS) is a very promising, sensitive method for the on-site identification of infectious pathogens based on scents, representing volatile organic compounds (VOCs). The purpose of this study was to prospectively assess whether identification of Influenza-A- infection based on VOCs by MCC-IMS is possible in breath. Nasal breath was investigated in 24 consecutive persons with and without Influenza-A-infection by MCC-IMS. In 14 Influenza-A-infected patients, infection was proven by PCR of nasopharyngeal swabs. Four healthy staff members and six patients with negative PCR result served as controls. For picking up relevant VOCs in MCC-IMS spectra, software based on cluster analysis followed by multivariate statistical analysis was applied. With only four VOCs canonical discriminant analysis was able to distinguish Influenza-A-infected patients from not infected with 100% sensitivity and 100% specificity. This present proof-of-concept- study yields encouraging results showing a rapid diagnosis of viral infections in nasal breath within 5 minutes by MCC-IMS. The next step is to validate the results with a greater number of patients with Influenza-A-infection as well as other viral diseases, especially COVID-19. Registration number at ClinicalTrials.gov NCT04282135.

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Rapid detection of SARS-CoV-2 infection by multicapillary column coupled ion mobility spectrometry (MCC-IMS) of breath. A proof of concept study

10.21203/rs.3.rs-70139/v1 ◽

2020 ◽

Author(s):

Claus Steppert ◽

Isabel Steppert ◽

William Sterlacci ◽

Thomas Bollinger

Keyword(s):

Ion Mobility ◽

Influenza A ◽

Viral Disease ◽

Pcr Analysis ◽

Proof Of Concept ◽

Influenza Epidemic ◽

Rt Pcr ◽

Respective Group ◽

Non Invasive ◽

Multicapillary Column

Abstract There is an urgent need for screening of patients having a communicable viral disease to cut infection chains. We could recently demonstrate that MCC-IMS of breath is able to identify Influenza-A infected patients. With decreasing Influenza epidemic and upcoming SARS-CoV-2 infections we went on and also analysed patients with suspected SARS-CoV-2 infections.75 patients, 34m, 41f, aged 64.4 ± 15.4 years, 14 positive for Influenza-A, 16 positive for SARS-CoV-2, the remaining 44 patients were used as controls. In one patient RT-PCR was highly suspicious of SARS-CoV-2 but initially inconclusiveBesides RT-PCR analysis of nasopharyngeal swabs all patients underwent MCC-IMS analysis of breath. There was no difference in gender or age according to the groups.97.3% of the patients could be correctly classified to the respective group by discriminant analysis. Even the inconclusive patient could be mapped to the SARS-CoV-2 group applying the discrimination function.ConclusionMCC-IMS is able to detect SARS-CoV-2 infection and Influenza-A infection in breath. As this method provides exact, fast non-invasive diagnosis it should be further developed for screening of communicable viral diseases.Trial registrationClinicalTrial.gov, NCT04282135 Registered 20 February 2020 - Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT04282135?term=IMS&draw=2&rank=1

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Rapid detection of SARS-CoV-2 infection by multicapillary column coupled ion mobility spectrometry (MCC-IMS) of breath. A proof of concept study

10.1101/2020.06.30.20143347 ◽

2020 ◽

Cited By ~ 2

Author(s):

Claus Steppert ◽

Isabel Steppert ◽

Gunther Becher ◽

William Sterlacci ◽

Thomas Bollinger

Keyword(s):

Discriminant Analysis ◽

Ion Mobility ◽

Influenza A ◽

Cross Validation ◽

Viral Disease ◽

Pcr Analysis ◽

Proof Of Concept ◽

Influenza Epidemic ◽

Rt Pcr ◽

Non Invasive

AbstractThere is an urgent need for screening patients of having a communicable viral disease to cut infection chains.We could recently demonstrate that MCC-IMS of breath is able to identify Influenza-A infected patients. With decreasing Influenza epidemic and upcoming SARS-CoV-2 infections we extended our study to the analysis of patients with suspected SARS-CoV-2 infections.51 patients, 23m, 28f, aged 64 ± 16 years, were included in this study.Besides RT-PCR analysis of nasopharyngeal swabs all patients underwent MCC-IMS analysis of breath. 16 patients, 7m, 9f, were positive for SARS-CoV-2 by RT-PCR. There was no difference in gender or age according to the groups.Stepwise canonical discriminant analysis could correctly classify the infected and non-infected subjects in 98% by cross-validation. Afterwards we combined the Influenza-A sub study and the SARS-CoV-2-sub study for a total of 75 patients, 34m, 41f, aged 64.8 ± 1.8 years, 14 positive for Influenza-A, 16 positive for SARS-CoV-2, the remaining 44 patients were used as controls. In one patient RT-PCR was highly suspicious of SARS-CoV-2 but inconclusive.There was no imbalance between the groups for age or gender.97.3% of the patients could be correctly classified to the respective group by discriminant analysis. Even the inconclusive patient could be mapped to the SARS-CoV-2 group applying the discrimination function.ConclusionMCC-IMS is able to detect SARS-CoV-2 infection and Influenza-A infection in breath. As this method provides exact, fast non-invasive diagnosis it should be further developed for screening of communicable viral diseases.Study registration: NCT04282135

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Static headspace-multicapillary column with gas chromatography coupled to ion mobility spectrometry as a simple approach for the discrimination of crude and processed traditional Chinese medicines

Journal of Separation Science ◽

10.1002/jssc.201400627 ◽

2014 ◽

Vol 37 (21) ◽

pp. 3090-3093 ◽

Cited By ~ 6

Author(s):

Gang Cao ◽

Qiyang Shou ◽

Qinglin Li ◽

Jianping Jiang ◽

Xiaocheng Chen

Keyword(s):

Gas Chromatography ◽

Ion Mobility Spectrometry ◽

Ion Mobility ◽

Simple Approach ◽

Traditional Chinese Medicines ◽

Static Headspace ◽

Chinese Medicines ◽

Multicapillary Column

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On-line headspace-multicapillary column-ion mobility spectrometry hyphenation as a tool for the determination of off-flavours in foods

Journal of Chromatography A ◽

10.1016/j.chroma.2014.01.062 ◽

2014 ◽

Vol 1333 ◽

pp. 99-105 ◽

Cited By ~ 15

Author(s):

Isabel Márquez-Sillero ◽

Soledad Cárdenas ◽

Stefanie Sielemann ◽

Miguel Valcárcel

Keyword(s):

Ion Mobility Spectrometry ◽

Ion Mobility ◽

On Line ◽

Multicapillary Column

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Detection of malignant pleural mesothelioma in exhaled breath by multicapillary column/ion mobility spectrometry (MCC/IMS)

Journal of Breath Research ◽

10.1088/1752-7155/10/4/046001 ◽

2016 ◽

Vol 10 (4) ◽

pp. 046001 ◽

Cited By ~ 10

Author(s):

Kevin Lamote ◽

Matthijs Vynck ◽

Joris Van Cleemput ◽

Olivier Thas ◽

Kristiaan Nackaerts ◽

...

Keyword(s):

Malignant Pleural Mesothelioma ◽

Ion Mobility Spectrometry ◽

Ion Mobility ◽

Pleural Mesothelioma ◽

Exhaled Breath ◽

Multicapillary Column

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Ion mobility spectrometry: the diagnostic tool of third millennium medicine

Revista da Associação Médica Brasileira ◽

10.1590/1806-9282.64.09.861 ◽

2018 ◽

Vol 64 (9) ◽

pp. 861-868 ◽

Cited By ~ 2

Author(s):

Katiuska I. Romero ◽

Roberto Fernandez-Maestre

Keyword(s):

Volatile Compounds ◽

Ion Mobility Spectrometry ◽

Ion Mobility ◽

Diagnostic Tool ◽

Biological Samples ◽

Low Cost ◽

Bronchial Carcinoma ◽

Chronic Obstructive ◽

Non Invasive ◽

Compact Size

SUMMARY Ion mobility spectrometry (IMS) is a fast, low cost, portable, and sensitive technique that separates ions in a drift tube under the influence of an electric field according to their size and shape. IMS represents a non-invasive and reliable instrumental alternative for the diagnosis of different diseases through the analysis of volatile metabolites in biological samples. IMS has applications in medicine in the study of volatile compounds for the non-invasive diagnose of bronchial carcinoma, chronic obstructive pulmonary disease, and other diseases analysing breath, urine, blood, faeces, and other biological samples. This technique has been used to study complex mixtures such as proteomes, metabolomes, complete organisms like bacteria and viruses, monitor anaesthetic agents, determine drugs, pharmaceuticals, and volatile compounds in human body fluids, and others. Pharmaceutical applications include analysis of over-the-counter-drugs, quality assessment, and cleaning verification. Medical practice needs non-invasive, robust, secure, fast, real-time, and low-cost methods with high sensitivity and compact size instruments to diagnose different diseases and IMS is the diagnostic tool that meets all these requirements of the Medicine of the future.

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