scholarly journals Effects of various carbohydrates on the in vitro pollen germination of Vinca rosea and Cucumis melo var. utilissimus

2020 ◽  
Author(s):  
P. Manchanda ◽  
S. Sharma ◽  
A.D. Marni
Keyword(s):  
Cucumis Melo ◽  
Pollen Germination ◽  
Primary Source ◽  
Germination Percentage ◽  
Hanging Drop ◽  
Vinca Rosea ◽  
Germination Medium ◽  
Hanging Drop Method ◽  
Pollen Germination Medium

AbstractPollen germination is crucial for the process of plant development which strongly depends on the presence of carbohydrates as a primary source of energy. In this study, we analyzed the differential effects of four sugars with varying concentrations on the pollen germination of Vinca rosea Linn. and Cucumis melo var. utilissimus Duth. & Fuller. using Brewbaker and Kwack’s medium as germination medium and hanging drop method after an incubation period of 1h. Sucrose and glucose supported the pollen germination but galactose and fructose did not support and even considerably inhibited the pollen germination of Vinca rosea. Whereas in pollen germination of Cucumis melo var. utilissimus, all the four sugars supported the pollen germination. The study suggests that 15% sucrose, for Vinca rosea, and 12% galactose, for Cucumis melo var. utilissimus, supports in achieving the highest pollen germination percentage when added to the pollen germination medium.

Development of pollen germination medium to test pollen viability of eggplant and its wild species

2018 ◽  
Vol 75 (2) ◽  
pp. 237
Author(s):  
P. Jayaprakash ◽  
D Sheeba ◽  
V.K. Vikas ◽  
M Sivasamy ◽  
T. Sabesan
Keyword(s):  
Wild Species ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Germination Medium ◽  
Pollen Germination Medium

Pollen viability in Kunzea pomifera (Myrtaceae) as influenced by sucrose concentration and storage

2006 ◽  
Vol 54 (6) ◽  
pp. 553 ◽  
Author(s):  
T. Page ◽  
G. M. Moore ◽  
J. Will ◽  
G. M. Halloran
Keyword(s):  
Pollen Germination ◽  
Storage Time ◽  
Sucrose Concentration ◽  
Pollen Viability ◽  
Germination Percentage ◽  
Liquid Media ◽  
Controlled Pollination ◽  
Fresh Pollen ◽  
And Storage

In vitro germination of freshly collected pollen was examined for five genotypes of Kunzea pomifera in liquid media with 5% and 10% sucrose. There were no significant differences in germination level between the different sucrose concentrations for each of the genotypes. Pollen-germination levels were significantly different among genotypes, ranging from 7 to 89%. Pollen of one genotype, stored for 12 months at 4°C and 10% relative humidity, was evaluated for germination at intervals over this period, and while significant differences in the levels of pollen germination after different storage periods were found, there was no correlation between germination percentage and storage time. Pollen of the K. pomifera accession Kmt1 stored under such conditions for 370 days was used in controlled pollination with accession Kmt2, resulting in 3.4 seeds per fruit, which was significantly fewer than the 17.2 seeds per fruit when using fresh pollen.

scholarly journals Optimized Protocol for In Vitro Pollen Germination in Yam (Dioscorea spp.)

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 795
Author(s):  
Jean M. Mondo ◽  
Paterne A. Agre ◽  
Robert Asiedu ◽  
Malachy O. Akoroda ◽  
Asrat Asfaw
Keyword(s):  
Pollen Germination ◽  
Incubation Temperature ◽  
Optimal Growth ◽  
Germination Percentage ◽  
Medium Composition ◽  
Assessment Protocol ◽  
Critical Problems ◽  
Growing Conditions

Yam (Dioscorea spp.) plants are mostly dioecious and sometimes monoecious. Low, irregular, and asynchronous flowering of the genotypes are critical problems in yam breeding. Selecting suitable pollen parents and preserving yam pollen for future use are potential means of controlling these constraints and optimizing hybridization practice in yam breeding programs. However, implementing such procedures requires a robust protocol for pollen collection and viability testing to monitor pollen quality in the field and in storage. This study, therefore, aimed at optimizing the pollen germination assessment protocol for yam. The standard medium composition was stepwisely modified, the optimal growth condition was tested, and in vivo predictions were made. This study showed that the differences in yam pollen germination percentage are primarily linked to the genotype and growing conditions (i.e., medium viscosity, incubation temperature, and time to use) rather than the medium composition. The inclusion of polyethylene glycol (PEG) in the culture medium caused 67–75% inhibition of germination in D. alata. Although the in vivo fertilization was dependent on female parents, the in vitro germination test predicted the percentage fruit set at 25.2–79.7% and 26.4–59.7% accuracy for D. rotundata and D. alata genotypes, respectively. This study provides a reliable in vitro yam pollen germination protocol to support pollen management and preservation efforts in yam breeding.

Effect of temperature on seed set and in vitro pollen germination in French beans (Phaseolus vulgaris)

1979 ◽  
Vol 19 (101) ◽  
pp. 725 ◽  
Author(s):  
PJ Farlow ◽  
DE Byth ◽  
NS Kruger
Keyword(s):  
Pollen Germination ◽  
Seed Set ◽  
Germination Percentage ◽  
French Bean ◽  
Hot Water ◽  
Effect Of Temperature ◽  
In Vitro Pollen Germination ◽  
French Beans ◽  
Different Temperatures

A technique for in vitro germination of French bean pollen was developed and the effect of temperature on gamete development, pollen germination and seed set investigated. Temperature had a profound effect on in vitro pollen germination percentage, pollen tube growth and bursting percentage. These were maximal at 7.2�C, 16.7�C and 38.3�C, respectively. In this study, pollen development was not affected at day/night temperatures of 16.1�/12.8�C, and ovule abortion was the cause of seed set failure at these temperatures. Hot water treatment (48�-44�C) of flowers caused failure of seed set due to pollen inviability. Consequently this technique may allow hybridization without emasculation in beans. Treatment of buds with hot water of different temperatures and in vitro pollen germination at high temperatures may have application as screening techniques for heat tolerance in French beans.

scholarly journals Factors Regulating In Vitro Germination of Date Palm Pollen Grams After Storage

2000 ◽  
Vol 5 (1) ◽  
pp. 19
Author(s):  
M.O. El Mardi ◽  
L. AI-Kharousi ◽  
O.S. AI-Mantheri
Keyword(s):  
Pollen Germination ◽  
Room Temperature ◽  
Date Palm ◽  
Pollen Grains ◽  
Germination Percentage ◽  
Liquid Media ◽  
Room Temperature Storage ◽  
Date Palm Pollen ◽  
Temperature Storage

Dried pollen grains of three date palm (male) cultivars grown in Oman were stored either in a freezer (-18°C), a refrigerator (4 to 5°C), or at room temperature (23 to 25°C) for 6 or 12 months. The three cultivars include Khori, Medjahdil, and Bahlani. Germination percentage was determined after 6 hours incubation in aerated and non- aerated liquid media. The results showed that date palm pollen was better adapted to refrigerator storage than freezing for all three cultivars, while room temperature storage significantly reduced pollen germination. Aeration was found to enhance germination of refrigerator- and freezer-stored pollen, but decreased that of pollen stored at room temperature for 6 months. The results also indicated that refrigeration might have caused the inactivation of pollen germination. The latter could either be overcome by aeration or reversed if pollen is refrigerated for a longer period. Room temperature storage directly reduced viability and did not induce temporary inactivation of pollen.

scholarly journals Pollen germination of the walnut cultivar ‛Geisenheim 286’ on different culture media

2014 ◽  
Vol 59 (1) ◽  
pp. 53-61
Author(s):  
Dragan Jankovic ◽  
Sladjana Jankovic ◽  
Svetlana Paunovic ◽  
Bratislav Cirkovic ◽  
Zoran Jovanovic
Keyword(s):  
Boric Acid ◽  
Calcium Chloride ◽  
Pollen Germination ◽  
Culture Media ◽  
Culture In Vitro ◽  
Agar Concentration ◽  
Germination Medium ◽  
Pollen Germinability

Pollen of the walnut cultivar ?Geisenheim 286(27.8%) when the germination medium contained 0.8% of agar, 15% of sucrose, 600 ppm of ? was cultured on germination media containing all possible combinations of sucrose (10, 15 and 20%); agar (0.6 and 0.8%); boric acid (0, 300 and 600 ppm) and calcium chloride (0, 50 and 100 ppm). A total of 54 different combinations of germination media were tested in an attempt to establish a suitable culture in vitro pollen germinability The interactions of the concentrations of agar and calcium chloride, boric acid and sucrose, calcium chloride and sucrose, as well as those of boric acid, calcium chloride and sucrose were significant. Pollen germination was maximized media for studying of the walnut. Significant differences in pollen germination were observed in response to changing concentrations of sucrose, boric acid and calcium chloride, but germination was not affected by changes in agar concentration. boric acid and 50 ppm of calcium chloride.

scholarly journals Pollen germination and pollen tube growth in Fraxinus pennsylvanica

Biologia ◽  
2006 ◽  
Vol 61 (1) ◽  
Author(s):  
Dario Kremer ◽  
Tomislav Jemrić
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Germination Percentage ◽  
Tube Length ◽  
Fraxinus Pennsylvanica ◽  
Green Ash ◽  
Optimum Temperature ◽  
Ecological Conditions ◽  
Pollen Tube Length

AbstractWith regard to adaptation of green ash (Fraxinus pennsylvanica Marshall) to ecological conditions in Croatia, pollen germination and pollen tube length after 2, 4 and 6 hours were examined in vitro at 10, 15, 20 and 25°C during two years 2001 and 2002. Narrow leaved ash (F. angustifolia Vahl) pollen served as a control in 2002. The year, time and temperature, and the interaction between time and temperature were significant for both germination percentage and pollen tube length. Interactions year × temperature and year × time were significant for pollen tube length only. The highest germination percentage (17.86% in 2001 and 19.40% in 2002) of green ash pollen was at 15°C after 6 hours. The pollen tube length was greatest at 20°C (393.46 µm) in 2001 and 25°C (899.50 µm) in 2002 after 6 hours. Narrow leaved ash pollen had the highest germination percentage (19.22%) at 20°C after 6 hours and was significantly reduced at 25°C. The pollen tube length was greatest at 25°C (518.90 µm) after 6 hours. It can be concluded that green ash pollen has satisfactory germination in ecological conditions in Croatia and that the optimum temperature for pollen germination is higher than 20°C.

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