scholarly journals “Candidatus Dechloromonas phosphatis” and “Candidatus Dechloromonas phosphovora”, two novel polyphosphate accumulating organisms abundant in wastewater treatment systems

2020 ◽  
Author(s):  
Francesca Petriglieri ◽  
Caitlin Singleton ◽  
Miriam Peces ◽  
Jette F. Petersen ◽  
Marta Nierychlo ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Batch Reactor ◽  
Carbon Sources ◽  
16S Rrna Gene Sequencing ◽  
Abundant Species ◽  
Rrna Gene ◽  
Biological Phosphorus Removal ◽  
Polyphosphate Accumulating Organisms

AbstractMembers of the genus Dechloromonas are often abundant in enhanced biological phosphorus removal (EBPR) systems and are recognized putative polyphosphate accumulating organisms (PAOs), but their role in phosphate (P) removal is still unclear. Here, we used 16S rRNA gene sequencing and fluorescence in situ hybridization (FISH) to investigate the abundance and distribution of Dechloromonas spp. in Danish wastewater treatment plants. Two species were abundant, novel, and uncultured, and could be targeted by existing FISH probes. Raman microspectroscopy of probe-defined organisms (FISH-Raman) revealed the levels and dynamics of important intracellular storage polymers in abundant Dechloromonas spp. in the activated sludge from four full-scale EBPR plants and from a lab-scale sequencing batch reactor fed with different carbon sources (acetate, glucose, glycine, and glutamate). Moreover, 7 distinct Dechloromonas species were determined from a set of 10 high-quality metagenome-assembled genomes (MAGs) from Danish EBPR plants, each encoding the potential for poly-P, glycogen, and polyhydroxyalkanoates (PHA) accumulation. The two most abundant species exhibited an in situ phenotype in complete accordance with the metabolic information retrieved by the MAGs, with dynamic levels of poly-P, glycogen, and PHA during feast-famine anaerobic-aerobic cycling, legitimately placing these microorganisms among the important PAOs. As no isolates are available for the two species, we propose the names Candidatus Dechloromonas phosphatis and Candidatus Dechloromonas phosphovora.

scholarly journals “Candidatus Dechloromonas phosphoritropha” and “Ca. D. phosphorivorans”, novel polyphosphate accumulating organisms abundant in wastewater treatment systems

2021 ◽  
Author(s):  
Francesca Petriglieri ◽  
Caitlin Singleton ◽  
Miriam Peces ◽  
Jette F. Petersen ◽  
Marta Nierychlo ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Niche Partitioning ◽  
16S Rrna Gene Sequencing ◽  
Abundant Species ◽  
Phosphate Removal ◽  
Rrna Gene ◽  
Biological Phosphorus Removal ◽  
Polyphosphate Accumulating Organisms

AbstractMembers of the genus Dechloromonas are often abundant in enhanced biological phosphorus removal (EBPR) systems and are recognized putative polyphosphate accumulating organisms (PAOs), but their role in phosphate removal is still unclear. Here, we used 16S rRNA gene sequencing and fluorescence in situ hybridization (FISH) to investigate the abundance and distribution of Dechloromonas spp. in Danish and global wastewater treatment plants. The two most abundant species worldwide revealed in situ dynamics of important intracellular storage polymers, measured by FISH-Raman in activated sludge from four full-scale EBPR plants and from a lab-scale reactor fed with different substrates. Moreover, seven distinct Dechloromonas species were determined from a set of ten high-quality metagenome-assembled genomes (MAGs) from Danish EBPR plants, each encoding the potential for polyphosphate (poly-P), glycogen, and polyhydroxyalkanoates (PHA) accumulation. The two species exhibited an in situ phenotype in complete accordance with the metabolic information retrieved by the MAGs, with dynamic levels of poly-P, glycogen, and PHA during feast-famine anaerobic–aerobic cycling, legitimately placing these microorganisms among the important PAOs. They are potentially involved in denitrification showing niche partitioning within the genus and with other important PAOs. As no isolates are available for the two species, we propose the names Candidatus Dechloromonas phosphoritropha and Candidatus Dechloromonas phosphorivorans.

scholarly journals In Situ Characterization ofNitrospira-Like Nitrite-Oxidizing Bacteria Active in Wastewater Treatment Plants

2001 ◽  
Vol 67 (11) ◽  
pp. 5273-5284 ◽  
Author(s):  
Holger Daims ◽  
Jeppe L. Nielsen ◽  
Per H. Nielsen ◽  
Karl-Heinz Schleifer ◽  
Michael Wagner
Keyword(s):  
Wastewater Treatment ◽  
16S Rrna ◽  
Laser Scanning ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Rrna Gene ◽  
Phylogenetic Affiliation

ABSTRACT Uncultivated Nitrospira-like bacteria in different biofilm and activated-sludge samples were investigated by cultivation-independent molecular approaches. Initially, the phylogenetic affiliation of Nitrospira-like bacteria in a nitrifying biofilm was determined by 16S rRNA gene sequence analysis. Subsequently, a phylogenetic consensus tree of theNitrospira phylum including all publicly available sequences was constructed. This analysis revealed that the genusNitrospira consists of at least four distinct sublineages. Based on these data, two 16S rRNA-directed oligonucleotide probes specific for the phylum and genus Nitrospira, respectively, were developed and evaluated for suitability for fluorescence in situ hybridization (FISH). The probes were used to investigate the in situ architecture of cell aggregates ofNitrospira-like nitrite oxidizers in wastewater treatment plants by FISH, confocal laser scanning microscopy, and computer-aided three-dimensional visualization. Cavities and a network of cell-free channels inside the Nitrospiramicrocolonies were detected that were water permeable, as demonstrated by fluorescein staining. The uptake of different carbon sources byNitrospira-like bacteria within their natural habitat under different incubation conditions was studied by combined FISH and microautoradiography. Under aerobic conditions, theNitrospira-like bacteria in bioreactor samples took up inorganic carbon (as HCO3 − or as CO2) and pyruvate but not acetate, butyrate, and propionate, suggesting that these bacteria can grow mixotrophically in the presence of pyruvate. In contrast, no uptake by theNitrospira-like bacteria of any of the carbon sources tested was observed under anoxic or anaerobic conditions.

Presence of Rhodocyclus in a full-scale wastewater treatment plant and their participation in enhanced biological phosphorus removal

2002 ◽  
Vol 46 (1-2) ◽  
pp. 123-128 ◽  
Author(s):  
J.L. Zilles ◽  
C.-H. Hung ◽  
D.R. Noguera
Keyword(s):  
Wastewater Treatment ◽  
Phosphorus Removal ◽  
Fluorescent In Situ Hybridization ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Polyphosphate Accumulating Organisms ◽  
Biological Phosphorus

The objective of this research was to assess the relevance of organisms related to Rhodocyclus in enhanced biological phosphorus removal in full-scale wastewater treatment plants. The presence of these organisms in full-scale plants was first confirmed by fluorescent in situ hybridization. To address which organisms were involved in phosphorus removal, a method was developed which selected polyphosphate-accumulating organisms from activated sludge samples by DAPI staining and flow cytometry. Sorted samples were characterized using fluorescent in situ hybridization. The results of these analyses confirmed the presence of organisms related to Rhodocyclus in full-scale wastewater treatment plants and supported the involvement of these organisms in enhanced biological phosphorus removal. However, a significant fraction of the polyphosphate-accumulating organisms were not related to Rhodocyclus.

scholarly journals Identification of a novel subgroup of uncultured gammaproteobacterial glycogen-accumulating organisms in enhanced biological phosphorus removal sludge

Microbiology ◽  
2011 ◽  
Vol 157 (8) ◽  
pp. 2287-2296 ◽  
Author(s):  
Jeong Myeong Kim ◽  
Hyo Jung Lee ◽  
Dae Sung Lee ◽  
Kangseok Lee ◽  
Che Ok Jeon
Keyword(s):  
Phosphorus Removal ◽  
Batch Reactor ◽  
Full Scale ◽  
Rrna Gene ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Phenotypic Properties ◽  
Polyphosphate Accumulating Organisms ◽  
Biological Phosphorus

The presence of glycogen-accumulating organisms (GAO) has been hypothesized to be a cause of deterioration in enhanced biological phosphorus removal (EBPR) processes due to their abilities to out-compete polyphosphate-accumulating organisms (PAO). Based on 16S rRNA gene sequences, new members of uncultured gammaproteobacterial GAO (GB) were identified from sludge samples of a lab-scale sequencing batch reactor used for EBPR. The new GB formed a phylogenetic lineage (GB8) clearly distinct from the previously reported seven GB subgroups. Because the new GB8 members were not targeted by the known fluorescence in situ hybridization (FISH) oligonucleotide probes, a GB8-specific FISH probe (GB429) and a new FISH probe (GB742) targeting all eight GB subgroups were designed, and the phenotypic properties of the new GB8 members were investigated. FISH and microautoradiography approaches showed that GB429-targeted cells (GB8) were large coccobacilli (2–4 µm in size) with the ability to take up acetate under anaerobic conditions, but unable to accumulate polyphosphate under the subsequent aerobic conditions, consistent with in situ phenotypes of GB. FISH analyses on several sludge samples showed that members of GB8 were commonly detected as the majority of GB in lab- and full-scale EBPR processes. In conclusion, this study showed that members of GB8 could be a subgroup of GB with an important role in EBPR deterioration. Designs of FISH probes which hybridize with broader GB subgroups at different hierarchical levels will contribute to studies of the distributions and ecophysiologies of GB in lab- or full-scale EBPR plants.

Substrate uptake by Gordonia amarae in activated sludge foams by FISH-MAR

2006 ◽  
Vol 54 (1) ◽  
pp. 39-45 ◽  
Author(s):  
E.L. Carr ◽  
K.L. Eales ◽  
R.J. Seviour
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Mycolic Acid ◽  
Substrate Uptake ◽  
Wastewater Treatment Process ◽  
Pure Cultures ◽  
Gordonia Amarae

Gordonia amarae is a right-angled branching filament belonging to the mycolic acid-containing Actinobacteria which is commonly found in many foaming activated sludge wastewater treatment plants. Although studies on different substrates as sole carbon sources by pure cultures of G. amarae have been carried out, none have examined substrate uptake by this organism in situ. Uptake of several hydrophilic and hydrophobic substrates by G. amarae was evaluated in situ using a combination of fluorescence in situ hybridization and microautoradiography. G. amarae could assimilate a range of both hydrophilic and hydrophobic substrates. From the data, G. amarae appears to be physiologically active under aerobic, anaerobic and anoxic condition (NO2 and NO3) for some substrates. This might explain why attempts to control foaming caused by G. amarae using anoxic and anaerobic selectors have been unsuccessful. This study emphasizes that bacteria can behave differently in situ to pure cultures and that it is important to evaluate the in situ physiology of these bacteria if we are to better understand their role in the wastewater treatment process.

Strategies for improvement of sludge quality and process performance of sequencing batch reactor plant treating municipal and tannery wastewater

1998 ◽  
Vol 38 (4-5) ◽  
pp. 69-77 ◽  
Author(s):  
M. Kabacinski ◽  
B. Hultman ◽  
E. Plaza ◽  
J. Trela
Keyword(s):  
Wastewater Treatment ◽  
Sequencing Batch Reactor ◽  
Wastewater Treatment Plants ◽  
Batch Reactor ◽  
Treatment Plant ◽  
Tannery Wastewater ◽  
Nitrification Rate ◽  
Process Performance ◽  
Biological Phosphorus Removal ◽  
Process Technology

Advanced process technology has been implemented at newly built wastewater treatment plants in Central and Eastern Europe. The wastewater treatment plant (WWTP) in Nowy Targ, Poland, the largest in Europe based on sequencing batch reactor (SBR) technology, has shown that newly constructed plants must be integrated into the system of water, wastewater, and sludge management in the city and the region. A significant supply of tannery wastewater with increasing chromium concentrations in the influent to the WWTP has resulted in many operational problems related mainly to sludge treatment. Evaluation of the process performance and sludge handling for 2 years of plant operation is presented. Efficient biological phosphorus removal with concentrations lower than 1 mg/l in effluent is obtained. Nitrogen removal is characterised by a low nitrification rate and a high denitrification rate. Problems with sludge handling are related to high excess sludge production, insufficient sludge stabilisation, low sludge dewatering efficiency and high chromium content in the sludge. Different strategies for sludge handling improvement are discussed. Sludge should be treated as a resource, which is recirculating in an eco-cycle with recovery of nutrients and energy. Such a process is proposed for the WWTP in Nowy Targ.

Polyphosphate-accumulating organisms in full-scale tropical wastewater treatment plants use diverse carbon sources

2019 ◽  
Vol 149 ◽  
pp. 496-510 ◽  
Author(s):  
Guanglei Qiu ◽  
Rogelio Zuniga-Montanez ◽  
Yingyu Law ◽  
Sara Swa Thi ◽  
Thi Quynh Ngoc Nguyen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Full Scale ◽  
Polyphosphate Accumulating Organisms

Sequencing Batch Reactors for Nutrient Removal at Small Wastewater Treatment Plants

1993 ◽  
Vol 28 (10) ◽  
pp. 233-242 ◽  
Author(s):  
Bjorn Rusten ◽  
Helge Eliassen
Keyword(s):  
Wastewater Treatment ◽  
Nutrient Removal ◽  
Wastewater Treatment Plants ◽  
Batch Reactor ◽  
Treatment Plant ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Biological Phosphorus Removal ◽  
Solids Retention ◽  
Co Precipitation

In order to optimize the sequencing batch reactor (SBR) process for nutrient removal at small wastewater treatment plants, a two year study was carried out at a treatment plant designed for 300 population equivalents. Different operating cycles, solids retention times (SRTs) and periods with co-precipitation were included in the test program. Based on the results, recommendations for achieving nitrification, denitrification and biological phosphorus removal were given. A suitable coagulant (and the optimum dosage) for co-precipitation was found as well.

scholarly journals Diverse Microbial Community Profiles of Propionate-Degrading Cultures Derived from Different Sludge Sources of Anaerobic Wastewater Treatment Plants

2020 ◽  
Vol 8 (2) ◽  
pp. 277 ◽  
Author(s):  
Pantakan Puengrang ◽  
Benjaphon Suraraksa ◽  
Peerada Prommeenate ◽  
Nimaradee Boonapatcharoen ◽  
Supapon Cheevadhanarak ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Biogas Production ◽  
16S Rrna Gene Sequencing ◽  
Anaerobic Sludge ◽  
Rrna Gene ◽  
Inside Information ◽  
Microbial Profile ◽  
Rrna Gene Sequencing ◽  
Enriched Cultures

Anaerobic digestion (AD) has been used for wastewater treatment and production of renewable energy or biogas. Propionate accumulation is one of the important problems leading to an unstable system and low methane production. Revealing propionate-degrading microbiome is necessary to gain a better knowledge for alleviation of the problem. Herein, we systematically investigated the propionate-degrading cultures enriched from various anaerobic sludge sources of agro-industrial wastewater treatment plants using 16S rRNA gene sequencing. Different microbial profiles were shown even though the methanogenic activities of all cultures were similar. Interestingly, non-classical propionate-degrading key players Smithella, Syntrophomonas, and Methanosaeta were observed as common prevalent taxa in our enriched cultures. Moreover, different hydrogenotrophic methanogens were found specifically to the different sludge sources. The enriched culture of high salinity sludge showed a distinct microbial profile compared to the others, containing mainly Thermovirga, Anaerolinaceae, Methanosaeta, Syntrophobactor, and Methanospirillum. Our microbiome analysis revealed different propionate-degrading community profiles via mainly the Smithella pathway and offers inside information for microbiome manipulation in AD systems to increase biogas production corresponding to their specific microbial communities.

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