scholarly journals Dysglycemia is associated with Mycobacterium tuberculosis lineages in tuberculosis patients of North Lima - Peru

2020 ◽  
Author(s):  
Kattya Lopez ◽  
María B. Arriaga ◽  
Juan G. Aliaga ◽  
Nadia N. Barreda ◽  
Oswaldo M. Sanabria ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Latin American ◽  
Tandem Repeats ◽  
Scientific Evidence ◽  
Variable Number ◽  
Health Care Facilities ◽  
Variable Number Tandem Repeats ◽  
Epidemiological Characteristics

AbstractThis study was performed to investigate the role of dysglycemia on the genetic diversity of Mycobacterium tuberculosis (MTB) among pulmonary tuberculosis (TB) patients to build scientific evidence about the possible mechanisms of TB transmission. MTB isolates obtained of patients affected by pulmonary tuberculosis from health care facilities of North Lima - Peru, were analyzed using whole genome sequencing and 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem repeats (MIRU-VNTR). Subsequently, clinical and epidemiological characteristics were associated with clustering, lineages and comorbid conditions. The analysis carried out 112 pulmonary TB patients from various health centers in North Lima, 17 (15%) had diabetes mellitus (DM) and 33 (29%) had pre-diabetes (PDM). Latin American-Mediterranean, Haarlem and Beijing were the most frequent MTB lineages found in those patients. Previous TB (adjusted odds ratio [aOR]=3.65; 95%CI: 1.32-17.81), age (aOR=1.12; 95%CI: 1.03-1.45) and Beijing lineage (aOR=3.53; 95%CI: 1.08-13.2) were associated with TB-DM comorbidity. Alcoholism (aOR=2.92; 95%CI: 1.10-8.28), age (aOR=1.05; 95%CI: 1.03-1.12) and Haarlem lineage (aOR=2.54; 95%CI: 1.04-6.51) were associated with TB-PDM comorbidity. Beijing and Haarlem lineages were independently associated with TB-DM and TB-PDM comorbidities, respectively. Although these findings may be surprising, we must be cautious to suggest that dysglycemia could be associated with a highly clustering and predisposition of MTB lineages related to a serious impact on the severity of TB disease, which requires further research.

scholarly journals Dysglycemia is associated with Mycobacterium tuberculosis lineages in tuberculosis patients of North Lima—Peru

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0243184
Author(s):  
Kattya Lopez ◽  
María B. Arriaga ◽  
Juan G. Aliaga ◽  
Nadia N. Barreda ◽  
Oswaldo M. Sanabria ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Latin American ◽  
Tandem Repeats ◽  
Scientific Evidence ◽  
Variable Number ◽  
Health Care Facilities ◽  
Variable Number Tandem Repeats ◽  
Epidemiological Characteristics

This study was performed to investigate the role of dysglycemia on the genetic diversity of Mycobacterium tuberculosis (MTB) among pulmonary tuberculosis (TB) patients to build scientific evidence about the possible mechanisms of TB transmission. MTB isolates obtained of patients affected by pulmonary tuberculosis from health care facilities of North Lima—Peru, were analyzed using whole genome sequencing and 24-locus mycobacterial interspersed repetitive-unit -variable-number tandem repeats (MIRU-VNTR). Subsequently, clinical and epidemiological characteristics were associated with clustering, lineages and comorbid conditions. The analysis carried out 112 pulmonary TB patients from various health centers in North Lima, 17 (15%) had diabetes mellitus (DM) and 33 (29%) had pre-diabetes (PDM). Latin American-Mediterranean, Haarlem and Beijing were the most frequent MTB lineages found in those patients. Previous TB (adjusted odds ratio [aOR] = 3.65; 95%CI: 1.32–17.81), age (aOR = 1.12; 95%CI: 1.03–1.45) and Beijing lineage (aOR = 3.53; 95%CI: 1.08–13.2) were associated with TB-DM comorbidity. Alcoholism (aOR = 2.92; 95%CI: 1.10–8.28), age (aOR = 1.05; 95%CI: 1.03–1.12) and Haarlem lineage (aOR = 2.54; 95%CI: 1.04–6.51) were associated with TB-PDM comorbidity. Beijing and Haarlem lineages were independently associated with TB-DM and TB-PDM comorbidities, respectively. Although these findings may be surprising, we must be cautious to suggest that dysglycemia could be associated with a highly clustering and predisposition of MTB lineages related to a serious impact on the severity of TB disease, which requires further research.

scholarly journals Detection of Mycobacterium Tuberculosis Multiple Strains in Sputum Samples from Patients with Pulmonary Tuberculosis in South Western Uganda using MIRU-VNTR

2021 ◽  
Author(s):  
Lisa Nkatha Micheni ◽  
Kennedy Kassaza ◽  
Hellen Kinyi ◽  
Ibrahim Ntulume ◽  
Joel Bazira
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Tandem Repeats ◽  
Variable Number ◽  
Disease Dynamics ◽  
Highly Sensitive ◽  
Risk Of Exposure ◽  
Multiple Strains ◽  
Western Uganda ◽  
Variable Number Tandem Repeats

Abstract Infections with multiple strains of Mycobacterium tuberculosis are now widely recognized as a common occurrence. Identification of patients infected with multiple strains, provides both insight into the disease dynamics and the epidemiology of tuberculosis. Analysis of Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeats (MIRU-VNTR) has been shown to be highly sensitive in detecting multiple M. tuberculosis strains even in sputum. The goal of this study was to identify cases of multiple M. tuberculosis strain infections among patients with pulmonary tuberculosis in south western Uganda and factors associated with multiple strain infections. Seventy-eight sputum samples were analyzed using the standard 24 loci MIRU-VNTR typing and an exact regression analysis performed using Stata version 14. Five (6.4%) of the 78 patients were infected with multiple strains of M. tuberculosis. All of the patients infected with multiple strains were the newly diagnosed cases while one third of them were co-infected with HIV. These findings point to a critical component of disease dynamics that is most likely being overlooked at the clinical level, emphasizing the need to further study the potential high risk of exposure to these categories of patients at the community level using a larger sample size.

scholarly journals Reverse zoonotic tuberculosis transmission from an emerging Uganda I strain between pastoralists and cattle in South-Eastern Nigeria

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Hezekiah Kehinde Adesokan ◽  
Victor Oluwatoyin Akinseye ◽  
Elizabeth Maria Streicher ◽  
Paul Van Helden ◽  
Rob Mark Warren ◽  
...  
Keyword(s):  
Public Health ◽  
Mycobacterium Bovis ◽  
Latin American ◽  
Disease Burden ◽  
Tandem Repeats ◽  
Variable Number ◽  
Molecular Tools ◽  
Tuberculosis Transmission ◽  
Public Health Challenge ◽  
Variable Number Tandem Repeats

Abstract Background Tuberculosis remains a major public health challenge globally with increasing risks for inter-transmission between pastoralists and cattle in Nigeria. This study was aimed at using molecular tools to establish zoonotic transmission of tuberculosis between pastoralists and their cattle in Ebonyi State, Nigeria. Sputum (n = 149) and milk (n = 144) samples from pastoralists and cattle, respectively were screened on the assumption of subclinical infections considering unguarded human-livestock interactions. Isolates obtained were analysed using deletion typing, spoligotyping and 24-Mycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeats (MIRU-VNTR). Results Fifty-four MTC were confirmed by deletion typing and were differentiated accordingly (M. tuberculosis: pastoralists =42, cattle = 2; M. bovis: pastoralists =1; M. africanum: pastoralists =9). Spoligotyping indicated 59.2% Uganda I/SIT46 (pastoralists =28; cattle = 1), 16.3% Latin American Mediterranean/SIT61 (pastoralists =8), 2.0% T/SIT53 (pastoralists =1) strains of M. tuberculosis and new strains of M. bovis and M. africanum. The 24-MIRU-VNTR of selected predominant cluster isolates shared by cattle and pastoralists (Uganda I/SIT46: pastoralists =9; cattle = 1) showed the same number of copies at each of the repetitive loci. Conclusions Mycobacterium bovis was confirmed in humans and a reverse zoonotic tuberculosis transmission from an emerging Uganda I M. tuberculosis strain between pastoralists and cattle in Nigeria evidenced by MIRU-VNTR. Using molecular tools will help mitigate disease burden through informed epidemiological insights.

Variable-number tandem repeats typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110 in Thailand

Tuberculosis ◽  
2010 ◽  
Vol 90 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Arunee Thong-On ◽  
Nat Smittipat ◽  
Tada Juthayothin ◽  
Hideki Yanai ◽  
Norio Yamada ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tandem Repeats ◽  
Variable Number ◽  
Copy Numbers ◽  
Variable Number Tandem Repeats

scholarly journals Polymorphism of Variable-Number Tandem Repeats at Multiple Loci in Mycobacterium tuberculosis

2005 ◽  
Vol 43 (10) ◽  
pp. 5034-5043 ◽  
Author(s):  
N. Smittipat ◽  
P. Billamas ◽  
M. Palittapongarnpim ◽  
A. Thong-On ◽  
M. M. Temu ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tandem Repeats ◽  
Variable Number ◽  
Multiple Loci ◽  
Variable Number Tandem Repeats

scholarly journals Characterisation of Neisseria meningitidis C strains causing two clusters in the north of Italy in 2007 and 2008

2009 ◽  
Vol 14 (16) ◽  
Author(s):  
C Fazio ◽  
A Neri ◽  
T Sofia ◽  
A Carannante ◽  
M G Caporali ◽  
...  
Keyword(s):  
Tandem Repeats ◽  
Clonal Complex ◽  
Fatal Outcome ◽  
Variable Number ◽  
The North ◽  
Surveillance Report ◽  
Laboratory Surveillance ◽  
Sporadic Cases ◽  
Variable Number Tandem Repeats

Two clusters of invasive meningococcal disease in the north of Italy both due to serogroup C/ST-11 clonal complex are here described. The objective of the investigation was to analyse the phenotype and the genotype of meningococci involved in the two clusters which were of national relevance due to the fatal outcome of the majority of cases (six of the total of 10 cases). All the strains were C:2a:P1.5 ST-11/ET-37 clonal complex. Two pulsed field gel electrophoresis (PFGE) and variable number tandem repeats (VNTR) profiles were identified, one for each cluster. VNTRs were different from those detected in Italy for C/ST-11 strains isolated from sporadic cases in the same period. This laboratory surveillance report highlights the importance and the crucial role of molecular characterisation to confirm the relatedness among meningococci responsible for clusters of cases.

scholarly journals Distribution of Mycobacterium tuberculosis lineages in South America

2021 ◽  
Vol 4 (3) ◽  
pp. 34-58
Author(s):  
Leslie Cabezas Vinueza ◽  
Patricia Jiménez Arias
Keyword(s):  
Mycobacterium Tuberculosis ◽  
South America ◽  
Latin American ◽  
Tandem Repeats ◽  
Control Program ◽  
Variable Number ◽  
Molecular Techniques ◽  
Complementary Oligonucleotide ◽  
Mdr Tb ◽  
The Impact

Molecular genotyping of Mycobacterium tuberculosis allows for the identification of circulating lineages and sublineages in the population and their relationship with migratory movements. The purpose of this review is to describe the phylogeography of Mycobacterium tuberculosis reported in South American countries that was analyzed using genotyping tools, analyze the Tuberculosis hotspots for the region and determine the impact of the COVID-19 pandemic on the Tuberculosis control program. The Latin American Mediterranean (LAM) sublineage belonging to the Euro-American lineage (Lineage 4) presents the highest prevalence in South America and is followed by the Beijing sublineage belonging to the East Asian lineage (Lineage 2). The Beijing sublineage is considered of worldwide interest because of its association with multidrug-resistant tuberculosis (MDR-TB), which is almost entirely distributed in South America, with Peru being the country with the highest prevalence for this sublineage. On the other hand, the Indo-Oceanic (Lineage 1), India-East Asia (Lineage 3) and West- African 2 (Lineage 6) sublineages have been reported with lower prevalence in South America. The molecular techniques used in the genotyping studies for Mycobacterium tuberculosis in South America were as follows: typing by complementary oligonucleotide spacer sequences (Spoligotyping), restriction-hybridization patterns (IS6110-RFLP, PGRS-RFLP), mycobacterial interspaced repeat units-variable number tandem repeats (MIRU-VNTR) and whole genome sequencing (WGS). At present, Brazil and Peru are the hotspots for tuberculosis and MDR-TB in South America, where the control of tuberculosis wholly affected by the COVID-19 pandemic. Thus, there have been significant impacts on containment programs and possible post-pandemic scenarios such that scientific contributions will need to be evaluated and implemented with new strategies for prevention, diagnosis, treatment and control of Tuberculosis.

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