scholarly journals Altered TGFB1 regulated pathways promote accelerated tendon healing in the superhealer MRL/MpJ mouse

2021 ◽  
Author(s):  
Jacob G. Kallenbach ◽  
Margaret A. T. Freeberg ◽  
David Abplanalp ◽  
Jacquelyn A. Myers ◽  
John M. Ashton ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Molecular Mechanisms ◽  
Cellular Proliferation ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Biomechanical Properties ◽  
Transcriptional Analysis ◽  
Flexor Tendon Repair ◽  
Cytokine Analysis ◽  
Mrl Mouse

AbstractTo better understand the molecular mechanisms of tendon healing, we investigated the Murphy Roth’s Large (MRL) mouse, which is considered a model of mammalian tissue regeneration. We show that compared to C57Bl/6J (C57) mice, injured MRL tendons have reduced fibrotic adhesions and cellular proliferation, with accelerated improvements in biomechanical properties. Transcriptional analysis of biological drivers showed positive enrichment of TGFB1 in both C57 and MRL healing tendons. However, only MRL tendons exhibited downstream transcriptional effects of cell cycle regulatory genes, with negative enrichment of the cell senescence-related regulators, compared to the positively-enriched inflammatory and ECM organization pathways in the C57 tendons. Serum cytokine analysis revealed decreased levels of circulating senescence-associated circulatory proteins (SASP) in response to injury in the MRL mice compared to the C57 mice. These data collectively demonstrate altered TGFB1 regulated inflammatory, fibrosis, and cell cycle pathways in flexor tendon repair.

Number of suture throws and its impact on the biomechanical properties of the four-strand cruciate locked flexor tendon repair with FiberWire

2012 ◽  
Vol 37 (9) ◽  
pp. 826-831 ◽  
Author(s):  
S. V. Le ◽  
S. Chiu ◽  
R. C. Meineke ◽  
P. Williams ◽  
M. D. Wongworawat
Keyword(s):  
Tensile Strength ◽  
Ultimate Tensile Strength ◽  
Flexor Tendon ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Early Mobilization ◽  
Biomechanical Properties ◽  
Optimal Number ◽  
Flexor Tendon Repair ◽  
Mode Of Failure

FiberWire is a popular suture in flexor tendon repair that allows for early mobilization, but its poor knot-holding properties have raised concerns over the potential effects on tendon healing and strength. We examined how the number of knot throws affects the 2 mm gap force, ultimate tensile strength, and mode of failure in a four-strand cruciate locked tendon repair in porcine flexor tendons in order to elucidate the optimal number of suture throws. There was no effect on the 2 mm gap force with increasing knot throws, but there was a significant increase in ultimate tensile strength. A minimum of six-knot throws prevents unravelling, whereas five out of 10 of repairs unravelled with less than six throws.

Gross and Histological Analysis of Healing After Dog Flexor Tendon Repair with the Teno Fix™ Device

2006 ◽  
Vol 31 (5) ◽  
pp. 524-529 ◽  
Author(s):  
B. W. SU ◽  
F. J. RAIA ◽  
H. M. QUITKIN ◽  
M. PARISIEN ◽  
R. J. STRAUCH ◽  
...  
Keyword(s):  
Flexor Tendon ◽  
Weight Bearing ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Full Weight Bearing ◽  
Cast Immobilization ◽  
Flexor Tendon Repair ◽  
The Common ◽  
Flexor Digitorum

The purpose of this study was to examine the in vivo characteristics of the stainless-steel Teno Fix™ device used for flexor tendon repair. The common flexor digitorum superficialis tendon was transected in 16 dogs and repaired with the device. The animals were euthanized at 3, 6, or 12 weeks postoperatively. Difficulties with cast immobilization led nine of 16 animals to be full weight bearing too early, leading to rupture of their repairs. The seven tendons with successful primary repairs (gap <2 mm) underwent histological examination. This in vivo study demonstrates that use of the Teno Fix™ in “suture” of dog flexor tendons did not lead to scarring at the tendon surface, does not cause an inflammatory reaction within the tendon and does not interfere with tendon healing.

Tendons

2011 ◽  
Author(s):  
David Warwick ◽  
Roderick Dunn ◽  
Erman Melikyan ◽  
Jane Vadher
Keyword(s):  
Tendon Rupture ◽  
Flexor Tendon ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Extensor Tendon ◽  
Suture Techniques ◽  
Flexor Tendon Repair ◽  
Tendon Suture ◽  
Anatomy And Physiology ◽  
Flexor Tendon Rupture

Anatomy and physiology 392Tendon healing 394Flexor tendon anatomy 396Flexor tendon zones of injury 400Flexor tendon suture techniques 402Flexor tendon repair 404Closed flexor tendon rupture 410Flexor tenolysis 412Flexor tendon reconstruction 414Extensor tendon anatomy 418Extensor tendon repair ...

Biomechanical Properties of a Novel Mesh Suture in a Cadaveric Flexor Tendon Repair Model

2019 ◽  
Vol 44 (3) ◽  
pp. 208-215 ◽  
Author(s):  
Stephen J. Wallace ◽  
Lauren M. Mioton ◽  
Robert M. Havey ◽  
Muturi G. Muriuki ◽  
Jason H. Ko
Keyword(s):  
Flexor Tendon ◽  
Tendon Repair ◽  
Biomechanical Properties ◽  
Flexor Tendon Repair

scholarly journals Early ultrasound therapy for rehabilitation after zone II flexor tendon repair

2014 ◽  
Vol 47 (01) ◽  
pp. 85-91 ◽  
Author(s):  
Krishnamurthy Geetha ◽  
Narayanan Chandramouli Hariharan ◽  
Jagannathan Mohan
Keyword(s):  
Flexor Tendon ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Early Mobilization ◽  
Ultrasound Therapy ◽  
Pulsed Ultrasound ◽  
Flexor Tendon Repair ◽  
Group 3 ◽  
Zone Ii ◽  
Ultrasound Group

ABSTRACT Background: In our Institute, most of the patients treated for hand injuries were industrial workers with poor compliance. For rehabilitation after zone II flexor tendon repair, we had tried various early mobilization protocols. As these protocols demanded a degree of commitment from the patients, our results were suboptimal. Hence, to improve the results, we implemented a new rehabilitation protocol by administering the pulsed ultrasound therapy during the early phase of tendon healing. Materials and Methods: This is a prospective study done over a period of five years from January 2008 to January 2013. A total of 100 patients and 139 digits with zone II flexor tendon injuries were studied. After randomization, we administered pulsed ultrasound therapy of different frequencies and intensities for a total of 72 patients and 99 digits and formulated three groups. The results of ultrasound treated cases were compared with each other and with the results of cases treated by immobilization protocol. The results were analyzed using ‘Original Strickland’ criteria. Results: 72% excellent-good results in ultrasound (Group 1) protocol, 75% excellent-good results in ultrasound (Group 2) protocol, and 77% excellent-good results in ultrasound (Group 3) protocol were achieved. There was no case of rupture in the first two groups. The rupture rate was 7% in ultrasound (Group 3) protocol. Only 25% excellent-good results were obtained in the immobilization protocol. Conclusion: After zone II flexor tendon repair, pulsed ultrasound therapy during the early rehabilitation phase is safe and effective. The results are comparable to early mobilization protocols.

Bone Marrow Derived Mesenchymal Stem Cell Augmentation of Rabbit Flexor Tendon Healing

Hand Surgery ◽  
2015 ◽  
Vol 20 (03) ◽  
pp. 421-429 ◽  
Author(s):  
Min He ◽  
Aaron Wei Tat Gan ◽  
Aymeric Yu Tang Lim ◽  
James Cho Hong Goh ◽  
James Hoi Po Hui ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Flexor Tendon ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Control Group ◽  
High Dose ◽  
Positive Staining ◽  
Flexor Tendon Repair

Background: This study investigated the effect of mesenchymal stem cell implantation on flexor tendon healing using a rabbit model of flexor tendon repair. Specifically, we compared the difference between autologous and allogeneic stem cells. The influence of cell number on the outcome of flexor tendon healing was also investigated. Methods: Repaired tendons on the rear paws of rabbits were randomly assigned into four groups: control group, 1 million autologous cells, 1 million allogeneic cells, and 4 million allogeneic cells. Rabbits were sacrificed at 3 or 8 weeks after surgery. Results: Implantation of 4 million stem cells resulted in a significant increase in range of motion compared with control group at three weeks after surgery. The positive staining of collagen I in healing tendons was enhanced in stem cell treated groups three weeks after surgery. However, stem cells did not improve biomechanical properties of flexor tendons. Conclusions: High dose stem cells attenuated adhesions in the early time point following flexor tendon repair. Further work is needed determine the value of stem cell therapy in flexor tendon healing in humans.

scholarly journals Defining the activation profile and fate trajectory of adult Scleraxis-lineage cells during tendon healing by combining lineage tracing and spatial transcriptomics

2021 ◽  
Author(s):  
Jessica E Ackerman ◽  
Katherine T Best ◽  
Samantha N Muscat ◽  
Chia-Lung Wu ◽  
Alayna E Loiselle
Keyword(s):  
Time Course ◽  
Molecular Mechanisms ◽  
Healing Process ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Cell Types ◽  
Healing Time ◽  
Lineage Tracing ◽  
Repair Site ◽  
Temporal And Spatial

The tendon healing process is regulated by the coordinated interaction of multiple cell types and molecular processes. However, these processes are not well-defined leading to a paucity of therapeutic approaches to enhance tendon healing. Scleraxis-lineage (ScxLin) cells are the major cellular component of adult tendon and make time-dependent contributions to the healing process. Prior work from our lab and others suggests heterogeneity within the broader ScxLin population over the course of tendon healing; therefore delineating the temporal and spatial contributions of these cells is critical to understanding and improving the healing process. In the present study we utilize lineage tracing of the adult ScxLin population to determine whether these cells undergo cellular activation and subsequent myofibroblast differentiation, which is associated with both proper healing and fibrotic progression in many tissues. We show that adult ScxLin cells undergo transient activation in the organized cellular bridge at the tendon repair site, contribute to the formation of an organized neo-tendon, and contribute to a persistent myofibroblast population in the native tendon stubs. The mechanisms dictating this highly specialized spatial response are unknown. We therefore utilized spatial transcriptomics to better define the spatio-molecular program of tendon healing. Integrated transcriptomic analyses across the healing time-course identifies five distinct molecular regions, including key interactions between the inflammatory bridging tissue and highly reactive tendon tissue at the repair site, with adult ScxLin cells being a central player in the transition from native tendon to reactive, remodeling tendon. Collectively, these data provide important insights into both the role of adult ScxLin cells during healing as well as the molecular mechanisms that underpin and coordinate the temporal and spatial healing phenotype, which can be leveraged to enhance the healing process.

The Effect of Seprafilm on Adhesion Formation and Tendon Healing After Flexor Tendon Repair in Chicken

Orthopedics ◽  
2010 ◽  
Vol 33 (3) ◽  
pp. 164-170 ◽  
Author(s):  
Erhan Yilmaz ◽  
Mustafa Avci ◽  
Mehmet Bulut ◽  
Halidun Kelestimur ◽  
Lokman Karakurt ◽  
...  
Keyword(s):  
Flexor Tendon ◽  
Adhesion Formation ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Flexor Tendon Repair
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