Bone Marrow Derived Mesenchymal Stem Cell Augmentation of Rabbit Flexor Tendon Healing

Hand Surgery ◽  
2015 ◽  
Vol 20 (03) ◽  
pp. 421-429 ◽  
Author(s):  
Min He ◽  
Aaron Wei Tat Gan ◽  
Aymeric Yu Tang Lim ◽  
James Cho Hong Goh ◽  
James Hoi Po Hui ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Flexor Tendon ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Control Group ◽  
High Dose ◽  
Positive Staining ◽  
Flexor Tendon Repair

Background: This study investigated the effect of mesenchymal stem cell implantation on flexor tendon healing using a rabbit model of flexor tendon repair. Specifically, we compared the difference between autologous and allogeneic stem cells. The influence of cell number on the outcome of flexor tendon healing was also investigated. Methods: Repaired tendons on the rear paws of rabbits were randomly assigned into four groups: control group, 1 million autologous cells, 1 million allogeneic cells, and 4 million allogeneic cells. Rabbits were sacrificed at 3 or 8 weeks after surgery. Results: Implantation of 4 million stem cells resulted in a significant increase in range of motion compared with control group at three weeks after surgery. The positive staining of collagen I in healing tendons was enhanced in stem cell treated groups three weeks after surgery. However, stem cells did not improve biomechanical properties of flexor tendons. Conclusions: High dose stem cells attenuated adhesions in the early time point following flexor tendon repair. Further work is needed determine the value of stem cell therapy in flexor tendon healing in humans.

Role of Platelets Rich Plasma (PRP) on the Outcome of Zone II Flexor Tendon Repair and Proximal Interphalangeal Joint (PIPJ) Range of Motion

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Ayman Gamal ◽  
Amr Magdy ◽  
Neven Mahmoud ◽  
Ahmad F El Sherif ◽  
Ahmed M Gad
Keyword(s):  
Range Of Motion ◽  
Statistical Difference ◽  
Flexor Tendon ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Control Group ◽  
Flexor Tendon Repair ◽  
Group I ◽  
Zone Ii ◽  
Group Ii

Abstract Background Flexor tendon injuries are a commonly occurring injury presenting to hand surgeons. Using PRP to augment tendon healing has been advocated only recently. Few studies discussed the effect of injection of PRP in tendon healing after tendon repair in large joints as regard functional outcome and range of motion (e.g. Achilles tendon and rotator cuff) Objective To study the effect of combination of PRP injection with physiotherapy on gliding and range of motion improvement after zone II flexor tendon repair. Patients and Methods prospective interventional controlled clinical study preformed on 40 patients with zone II flexor tendon injury. Patients were divided into two groups, group I (interventional group) PRP was injected around the tendon at the site of repair after one and six weeks of repair and patients received physiotherapy according to our unit protocol. In group II (control group) patients received physiotherapy only. Both groups were evaluated at six and twelve weeks regarding the range of motion according to Buck-Gramko II criteria. Results Based on the Buck-Gramcko II criteria, the overall results ranged between fair and good in both groups after six weeks of repair, the score ranged between 7-16 (mean= 11.5) in group I and 6-14 (mean= 10) in group II, There was no statistical difference between the two groups (P = 0.46).After 12 weeks of repair, the overall results ranged between good and excellent in both groups, the score ranged between 11-17 (mean= 14) in group I and 9-16 (mean=12.5) in group II. There was no statistical difference between the two groups (p = 0.207). Conclusion PRP injection had no statistical significant effect of on the gliding after flexor tendon repair at zone II.

Gross and Histological Analysis of Healing After Dog Flexor Tendon Repair with the Teno Fix™ Device

2006 ◽  
Vol 31 (5) ◽  
pp. 524-529 ◽  
Author(s):  
B. W. SU ◽  
F. J. RAIA ◽  
H. M. QUITKIN ◽  
M. PARISIEN ◽  
R. J. STRAUCH ◽  
...  
Keyword(s):  
Flexor Tendon ◽  
Weight Bearing ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Full Weight Bearing ◽  
Cast Immobilization ◽  
Flexor Tendon Repair ◽  
The Common ◽  
Flexor Digitorum

The purpose of this study was to examine the in vivo characteristics of the stainless-steel Teno Fix™ device used for flexor tendon repair. The common flexor digitorum superficialis tendon was transected in 16 dogs and repaired with the device. The animals were euthanized at 3, 6, or 12 weeks postoperatively. Difficulties with cast immobilization led nine of 16 animals to be full weight bearing too early, leading to rupture of their repairs. The seven tendons with successful primary repairs (gap <2 mm) underwent histological examination. This in vivo study demonstrates that use of the Teno Fix™ in “suture” of dog flexor tendons did not lead to scarring at the tendon surface, does not cause an inflammatory reaction within the tendon and does not interfere with tendon healing.

scholarly journals The The Influence of Mesenchymal Stem Cell Wharton Jelly toward Prostaglandin E2 Gene Expression on Synoviocyte Cell Osteoarthritis

2019 ◽  
Vol 7 (8) ◽  
pp. 1252-1258 ◽  
Author(s):  
Vivi Sofia ◽  
Moch Saiful Bachri ◽  
Rizki Rahmadian
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Prostaglandin E2 ◽  
Expression Analysis ◽  
Control Group ◽  
E2 Gene

BACKGROUND: Pharmacological therapy in the management of OA causes many new health problems due to side effects caused by long-term use of drugs, such as long-term use of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) will cause gastric ulcers and impaired kidney function. In OA pathogenesis, PGE2 gene is involved in the inflammation process. AIM: This study aims to identify the influence of Wharton Jelly Mesenchymal Stem Cell (MSC-WJ) on PGE2 expression gene in synoviocyte by in vitro. MATERIAL AND METHODS: The method used in this study is the co-culture method of primary cells and stem cells in the appropriate media. This research is pure experimental research. The sample used came from synovial tissue of osteoarthritis patients who underwent Total Knee Replacement (TKR) surgery. This study was divided into 6 groups treated with 4 replications. The expression analysis of the Prostaglandin E2 gene was done using qPCR (Real-Time Polymerase Chain Reaction). The expression analysis of the Prostaglandin E2 gene was carried out before and after the co-culture with Wharton's Jelly and continued with the analysis of statistical data processing using the SPSS.15 program. PGE2 gene expression data were processed using the Kruskal-Wallis test and continued with the Mann-Whitney test with a 95% confidence level. RESULTS: The results showed that Mesenchymal Stem Cells Wharton Jelly could reduce the expression of Prostaglandin E2 gene after co-culture for 24 hours and 48 hours in synoviocyte cells osteoarthritis significantly compared with the control group. The administration of Mesenchymal Stem Cells for 24 hours reduced the expression level of PGE2 gene by 0.61 times compared to the control group (p < 0.05) and the administration of Mesenchymal Stem Cells for 48 hours decreased the expression level of PGE2 gene by 0, 47 times compared to the control group (p < 0.05). CONCLUSION: This study concluded that MSC-WJ in OA synoviocyte significantly reduced the expression of the PGE2 gene (p < 0.05).

Tendons

2011 ◽  
Author(s):  
David Warwick ◽  
Roderick Dunn ◽  
Erman Melikyan ◽  
Jane Vadher
Keyword(s):  
Tendon Rupture ◽  
Flexor Tendon ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Extensor Tendon ◽  
Suture Techniques ◽  
Flexor Tendon Repair ◽  
Tendon Suture ◽  
Anatomy And Physiology ◽  
Flexor Tendon Rupture

Anatomy and physiology 392Tendon healing 394Flexor tendon anatomy 396Flexor tendon zones of injury 400Flexor tendon suture techniques 402Flexor tendon repair 404Closed flexor tendon rupture 410Flexor tenolysis 412Flexor tendon reconstruction 414Extensor tendon anatomy 418Extensor tendon repair ...

Growth Factor and Intense Pulse Light in Flexor Tendon Repair: A Biomechanical Study at Strength and Gap Resistance

2018 ◽  
Vol 23 (04) ◽  
pp. 463-468
Author(s):  
Rohit Singh ◽  
Jihad Alzyoud ◽  
Ryan Trickett ◽  
Peter Thomas ◽  
Peter Theobald ◽  
...  
Keyword(s):  
Growth Factor ◽  
Flexor Tendon ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Lead Author ◽  
Control Group ◽  
Intense Pulse Light ◽  
Vast Number ◽  
Collagen Formation ◽  
Pulse Light

Background: Flexor tendon injuries are extremely common and they are usually the result of incised traumatic glass or knife injury. The process of tendon healing is a complicated and exceptionally-regimented mechanism that is originated and monitored by a vast number of diverse molecules. One of the most pivotal groups of mediators that are crucial to the healing process are growth factors (GF). Intense pulse light (IPL) can lead to evidence of new collagen formation with associated clinical improvement in tissue healing. The biological benefit of Intense pulse light (IPL) relies on judicious photothermolysis, where heat driven radiation is dissipated and focused at the cellular level. The aims of this study is to set out the effect of growth factor and IPL on healing following a tendon repair. Methods: Bovine common digital extensor tendons (CDET) were used as an ex vivo model. 44 tendon repairs were performed by the lead author using 2.5 × magnification loupes and standard instruments. Clamped tendons were assigned into the following groups; control, IPL, GF, IPL and GF. After culturing, biomechanical testing was carried out using monotonic tensile testing with displacement-controlled uniaxial tension to failure. Results: The mean values for ultimate tensile stress (UTS) for the control group was 53.51 N, for IPL it was 51.15 N, for growth factor was 70.10 N and for combined growth factor and IPL it was 75.16 N. Conclusions: This study showed significant improvement in UTS when repaired tendons were cultured with growth factor compared to control and IPL. This would suggest a biomechanical advantage for tendon healing.

scholarly journals Early ultrasound therapy for rehabilitation after zone II flexor tendon repair

2014 ◽  
Vol 47 (01) ◽  
pp. 85-91 ◽  
Author(s):  
Krishnamurthy Geetha ◽  
Narayanan Chandramouli Hariharan ◽  
Jagannathan Mohan
Keyword(s):  
Flexor Tendon ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Early Mobilization ◽  
Ultrasound Therapy ◽  
Pulsed Ultrasound ◽  
Flexor Tendon Repair ◽  
Group 3 ◽  
Zone Ii ◽  
Ultrasound Group

ABSTRACT Background: In our Institute, most of the patients treated for hand injuries were industrial workers with poor compliance. For rehabilitation after zone II flexor tendon repair, we had tried various early mobilization protocols. As these protocols demanded a degree of commitment from the patients, our results were suboptimal. Hence, to improve the results, we implemented a new rehabilitation protocol by administering the pulsed ultrasound therapy during the early phase of tendon healing. Materials and Methods: This is a prospective study done over a period of five years from January 2008 to January 2013. A total of 100 patients and 139 digits with zone II flexor tendon injuries were studied. After randomization, we administered pulsed ultrasound therapy of different frequencies and intensities for a total of 72 patients and 99 digits and formulated three groups. The results of ultrasound treated cases were compared with each other and with the results of cases treated by immobilization protocol. The results were analyzed using ‘Original Strickland’ criteria. Results: 72% excellent-good results in ultrasound (Group 1) protocol, 75% excellent-good results in ultrasound (Group 2) protocol, and 77% excellent-good results in ultrasound (Group 3) protocol were achieved. There was no case of rupture in the first two groups. The rupture rate was 7% in ultrasound (Group 3) protocol. Only 25% excellent-good results were obtained in the immobilization protocol. Conclusion: After zone II flexor tendon repair, pulsed ultrasound therapy during the early rehabilitation phase is safe and effective. The results are comparable to early mobilization protocols.

Efficacy of Adcon-T/N After Primary Flexor Tendon Repair in Zone II: A Controlled Clinical Trial

2003 ◽  
Vol 28 (2) ◽  
pp. 113-115 ◽  
Author(s):  
A. GOLASH ◽  
A. KAY ◽  
J. G. WARNER ◽  
F. PECK ◽  
J. S. WATSON ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Flexor Tendon ◽  
Tendon Repair ◽  
Tendon Sheath ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Flexor Tendon Repair ◽  
Double Blind ◽  
Active Motion ◽  
Zone Ii

A prospective double-blind, randomized, controlled clinical trial was conducted to assess the use of ADCON-T/N after flexor tendon repair in Zone II. Forty-five patients with 82 flexor tendon repairs in 50 digits completed the study. ADCON-T/N was injected into the tendon sheath after tenorrhaphy in the experimental group while the control group was not treated with ADCON-T/N. ADCON-T/N had no statistically significant effect on total active motion at 3, 6 and 12 months but the time taken to achieve the final range of motion was significantly shorter in treated patients. ADCON-treated patients had a higher rupture rate but this was not significant.

The Effect of Seprafilm on Adhesion Formation and Tendon Healing After Flexor Tendon Repair in Chicken

Orthopedics ◽  
2010 ◽  
Vol 33 (3) ◽  
pp. 164-170 ◽  
Author(s):  
Erhan Yilmaz ◽  
Mustafa Avci ◽  
Mehmet Bulut ◽  
Halidun Kelestimur ◽  
Lokman Karakurt ◽  
...  
Keyword(s):  
Flexor Tendon ◽  
Adhesion Formation ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Flexor Tendon Repair

scholarly journals Stem Cells for Augmenting Tendon Repair

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Lawrence V. Gulotta ◽  
Salma Chaudhury ◽  
Daniel Wiznia
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Growth Factors ◽  
Adhesion Formation ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Scar Tissue ◽  
Mechanical Stimuli ◽  
Injury Site ◽  
Attractive Option

Tendon healing is fraught with complications such as reruptures and adhesion formation due to the formation of scar tissue at the injury site as opposed to the regeneration of native tissue. Stem cells are an attractive option in developing cell-based therapies to improve tendon healing. However, several questions remain to be answered before stem cells can be used clinically. Specifically, the type of stem cell, the amount of cells, and the proper combination of growth factors or mechanical stimuli to induce differentiation all remain to be seen. This paper outlines the current literature on the use of stem cells for tendon augmentation.

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